FastQCFastQC Report
Wed 25 May 2016
SRR1295172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596687
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA171272.8703491110079487No Hit
GTACATAAGCAGTGGTATCAACGCA117341.9665251631089669No Hit
GCTTATGTACTCTGCGTTGATACCA71151.1924174651031445No Hit
GTATCAACGCAGAGTACTTTTTTTT53180.8912545438395675No Hit
CTTATGTACTCTGCGTTGATACCAC49360.8272343791636151No Hit
GGTATCAACGCAGAGTACTTTTTTT37460.6277998347542346No Hit
GAGTACATAAGCAGTGGTATCAACG33460.5607630131040227No Hit
TATCAACGCAGAGTACTTTTTTTTT33250.5572435799673866No Hit
TATGTACTCTGCGTTGATACCACTG30110.5046196749719702No Hit
GTGGTATCAACGCAGAGTACATGGG28470.47713457809538334No Hit
GTACTGGTTCACTATCGGTCAGTCA24750.4147903339606862No Hit
ATACAGGGTGACAGCCCCGTACACA24370.4084218359039161No Hit
GCGTTGATACCACTGCTTATGTACT24100.4038968504425268No Hit
CCCATGTACTCTGCGTTGATACCAC24000.4022209299012715No Hit
CATAAGCAGTGGTATCAACGCAGAG22310.373897872754057No Hit
GGATACCACGTGTCCCGCCCTACTC20230.33903872549594677No Hit
ACGCAGAGTACATAAGCAGTGGTAT19620.32881561019428945No Hit
CTGCTTATGTACTCTGCGTTGATAC18990.3182573107843811No Hit
CATGTACTCTGCGTTGATACCACTG18800.31507306175599603No Hit
ACTCTGCGTTGATACCACTGCTTAT17820.2986490404516941No Hit
GTTAATGATAGTGTGTCGAAACACA17070.2860796363922794No Hit
GTATCAACGCAGAGTACATAAGCAG16090.2696556150879775No Hit
ACCCTGTATCGCGCGCCTTTCCAGA16080.26948802303385194No Hit
ATCATTAACTGAATCCATAGGTTAA15650.26228156470645414No Hit
GGTATCAACGCAGAGTACATAAGCA15230.25524269843318187No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14490.24284088642789267No Hit
TATCAACGCAGAGTACATAAGCAGT14020.23496405988399277No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13560.22725482539421843No Hit
GGTATCAACGCAGAGTACATGGGGG12130.20328916165426764No Hit
CCCATATTCAGACAGGATACCACGT11950.2002725046800081No Hit
GGTTAATGAGGCGAACCGGGGGAAC11850.19859658413875278No Hit
AAGCAGTGGTATCAACGCAGAGTAC11620.19474196689386564No Hit
GTACATGGGAAGCAGTGGTATCAAC11190.18753550856646783No Hit
TCTAAGTACCCCGAGGAAAAGAAAT11120.18636236418758914No Hit
GTGGTATCAACGCAGAGTACATAAG11030.18485403570045938No Hit
GTATCAACGCAGAGTACATGGGGGT10990.18418366748395726No Hit
GTATTTAGCCTTGGAGGATGGTCCC10800.1809994184555722No Hit
ACTTAGATGTTTCAGTTCCCCCGGT10600.17764757737306158No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10360.17362536807404888No Hit
GCCTTGGAGGATGGTCCCCCCATAT10060.168597606450283No Hit
GTACATGATAAGCAGTGGTATCAAC10050.16843001439615746No Hit
ACATAAGCAGTGGTATCAACGCAGA9940.1665865018007766No Hit
ATCATGTACTCTGCGTTGATACCAC9790.16407262098889366No Hit
GCCCAGAGCCTGAATCAGTGTGTGT9750.16340225277239157No Hit
GTTCACTATCGGTCAGTCAGGAGTA9510.15938004347337886No Hit
TATTCAGACAGGATACCACGTGTCC9400.15753653087799802No Hit
CACACACACTGATTCAGGCTCTGGG9330.1563633864991193No Hit
GGGTTTCCCCATTCGGAAATCGCCG8870.14865415200934493No Hit
CATCTAAGTACCCCGAGGAAAAGAA8530.1429560221690769No Hit
GTACTCTGCGTTGATACCACTGCTT8530.1429560221690769No Hit
GCAGTGGTATCAACGCAGAGTACAT8510.14262083806082587No Hit
GTTATAACCGGCGATTTCCGAATGG8410.14094491751957058No Hit
GTACATGGGGGTTAAGCGACTAAGC8320.1394365890324408No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8170.13692270822055785No Hit
GATACCACTGCTTATGTACTCTGCG8100.13574956384167913No Hit
CCACTGCTTATGTACTCTGCGTTGA8030.1345764194628004No Hit
GTCAGGAGTATTTAGCCTTGGAGGA7880.1320625386509175No Hit
CTATCGGTCAGTCAGGAGTATTTAG7860.13172735454266643No Hit
TCATTAACCTATGGATTCAGTTAAT7780.1303866181096622No Hit
AGCGTACACGGTGGATGCCCTGGCA7670.12854310551428136No Hit
TTCCAGACGCTTCCACTAACACACA7670.12854310551428136No Hit
GTGGATGCCCTGGCAGTCAGAGGCG7650.1282079214060303No Hit
GTACTTAGATGTTTCAGTTCCCCCG7580.12703477702715157No Hit
GTGCTAATCTGCGATAAGCGTCGGT7520.1260292247023984No Hit
TCACGGTACTGGTTCACTATCGGTC7400.12401812005289205No Hit
ATACCACTGCTTATGTACTCTGCGT7390.12385052799876652No Hit
CGGTACTGGTTCACTATCGGTCAGT7340.12301256772813889No Hit
CCCCAGTAGCGGCGAGCGAACGGGG7320.12267738361988782No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7240.12133664718688357No Hit
GTTGATACCACTGCTTATGTACTCT7140.11966072664562827No Hit
CCTATGGATTCAGTTAATGATAGTG6910.1158061094007411No Hit
AGCTCACAGCATGTGCATTTTTGTG6890.11547092529249003No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN6850.11480055707598791No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC6600.11061075572284967No Hit
CTACTGGGGGAATCTCGGTTGATTT6590.11044316366872414No Hit
GAATCAGTGTGTGTGTTAGTGGAAG6530.10943761134397097No Hit
ACGCAGAGTACATGGGGGTTAAGCG6290.10541540204495824No Hit
GCCTGAATCAGTGTGTGTGTTAGTG6250.10474503382845612No Hit
GTCCCGCCCTACTCATCGAGCTCAC6240.1045774417743306No Hit
GCCCTACTCATCGAGCTCACAGCAT6230.10440984972020506No Hit
GATTCAGGCTCTGGGCTGCTCCCCG6170.10340429739545189No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6090.10206356096244765No Hit
ATGATAGTGTGTCGAAACACACTGG6090.10206356096244765No Hit
AAACAAAAAAAAAAAAAAAAAAAAA6080.10189596890832212No Hit
GGTATCAACGCAGAGTACATGGGGA6030.10105800863769447No Hit
TATCAACGCAGAGTACATGGGGGTT5980.10022004836706681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAA250.006030878518.99831811
GTCGCCT350.002169363216.28427111
TGTAGTT350.002169363216.28427115
GTACAAA755.7947545E-815.2063271
GGGTCCT456.758181E-414.7764697
CTCGTGT400.005274186414.24993614
CCAGCCG400.005274186414.24993614
TCTCGTG400.005274186414.24993613
TCGTGTC400.005277130814.24873815
GTTCCTT400.005277130814.2487386
CGGTCTC400.005277130814.24873810
TCAGCAA551.9584197E-413.81695810
CCTGACT551.9640164E-413.81231318
TGGGTCC500.001498648813.2988216
CGAATGG1500.013.29435119
GTCTTCG604.0711023E-412.671941
GGGCAAG604.0884773E-412.6655467
TCGGTCT604.0884773E-412.66554615
CGGTCTT604.0913795E-412.66448116
TACAGGG4650.012.4654552
Produced by FastQC (version 0.11.5)