##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295172_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	596687
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.852518992369532	31.0	28.0	33.0	16.0	34.0
2	28.821852998305644	31.0	28.0	33.0	16.0	34.0
3	29.246579865155432	31.0	29.0	34.0	20.0	34.0
4	32.00894606384922	35.0	32.0	37.0	17.0	37.0
5	33.02368075724794	35.0	33.0	37.0	25.0	37.0
6	33.04665092418638	36.0	33.0	37.0	23.0	37.0
7	32.94286284098698	36.0	33.0	37.0	23.0	37.0
8	32.96530006519331	36.0	33.0	37.0	23.0	37.0
9	33.659645676879165	38.0	33.0	39.0	17.0	39.0
10	33.817840844529876	38.0	33.0	39.0	17.0	39.0
11	34.20941297531201	38.0	34.0	39.0	21.0	39.0
12	34.30552199059138	39.0	34.0	39.0	22.0	39.0
13	34.08557920651866	38.0	34.0	39.0	19.0	39.0
14	34.64355181192149	39.0	33.0	41.0	17.0	41.0
15	34.933814545984745	39.0	33.0	41.0	18.0	41.0
16	34.899344212292206	39.0	33.0	41.0	19.0	41.0
17	34.73681176228072	39.0	33.0	41.0	18.0	41.0
18	34.605422943687394	38.0	33.0	41.0	18.0	41.0
19	34.99503424743626	39.0	34.0	41.0	21.0	41.0
20	35.02075962774453	39.0	34.0	41.0	21.0	41.0
21	35.09614923737236	39.0	34.0	41.0	21.0	41.0
22	35.0411388215262	39.0	33.0	41.0	22.0	41.0
23	34.80583454977233	39.0	33.0	41.0	21.0	41.0
24	35.13985054140613	39.0	33.0	41.0	24.0	41.0
25	34.065196660895914	38.0	31.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5260337892392499
1101	2	0.5697493932899782
1101	3	0.555176177485766
1101	4	0.5897115585847956
1101	5	0.5491874548235884
1101	6	0.5207268522892576
1101	7	0.4623107483740938
1101	8	0.5424367308960711
1101	9	0.8291015467691096
1101	10	1.0641628072604163
1101	11	0.8694581969293651
1101	12	0.9119242235455758
1101	13	0.8282834274524475
1101	14	1.0965196221182154
1101	15	1.0274098384813968
1101	16	0.7938953912400493
1101	17	1.0808844848501735
1101	18	0.8761909819531297
1101	19	0.5449432206255338
1101	20	0.7757149564559356
1101	21	0.9210759356434153
1101	22	0.8565989511526695
1101	23	0.989870294219017
1101	24	0.7191681762982753
1101	25	0.7890329607073454
1102	1	-0.3097348831712097
1102	2	0.03079715776416947
1102	3	-0.18684350847809128
1102	4	-0.15115478469617116
1102	5	-0.3433207476613518
1102	6	-0.2315769140427406
1102	7	-0.4541335311629595
1102	8	-0.342710317052628
1102	9	-0.04874402466742822
1102	10	0.30047610650808565
1102	11	0.21429598297845587
1102	12	-0.02051765849172682
1102	13	-0.15813463267446082
1102	14	0.1953529167473178
1102	15	-0.028199701180483316
1102	16	0.15240074215331845
1102	17	-0.1725174868598529
1102	18	0.2110703220698653
1102	19	0.08946237791994349
1102	20	0.16250898813459003
1102	21	0.16956744384978606
1102	22	0.09688473104714035
1102	23	-0.0952824620624213
1102	24	-0.04225724628051353
1102	25	-0.29887147142382275
1103	1	-0.21901627889021924
1103	2	0.040412818374402804
1103	3	-4.471240708561197E-4
1103	4	-0.4002773719742727
1103	5	-0.08698967668290436
1103	6	-0.4613063969367772
1103	7	-0.5874467720711394
1103	8	-0.27338362204780964
1103	9	0.3739340607593036
1103	10	0.4638718829237547
1103	11	-0.012103898032634675
1103	12	-0.008361741272516099
1103	13	-0.18229773097860402
1103	14	-0.008265546364810916
1103	15	-0.045254777910066935
1103	16	-0.3246038686668413
1103	17	-0.07318653852347978
1103	18	0.18515109361899107
1103	19	0.014137963535851839
1103	20	-0.006684888111728071
1103	21	-0.035859337526360946
1103	22	0.12008104447853896
1103	23	-2.213202817776633
1103	24	-0.9619475619925311
1103	25	-0.18822483455657135
1104	1	-0.30167964726787133
1104	2	-0.24773998894216476
1104	3	-0.44240779599668656
1104	4	-0.9873275652962441
1104	5	-0.22983173590127137
1104	6	-0.30359689776386745
1104	7	-0.4050302725814703
1104	8	-0.5089372670091237
1104	9	-2.424365007574611
1104	10	-0.3345403127009874
1104	11	-0.6043629746122718
1104	12	-0.29974244728737176
1104	13	-0.29034434947818966
1104	14	-0.19809062643379605
1104	15	-0.24501746199330654
1104	16	-0.3163606600752047
1104	17	0.1736471543032465
1104	18	0.23372041048099135
1104	19	0.014467289649154225
1104	20	-0.14975068797195235
1104	21	-0.23075252022442072
1104	22	-0.23893721175226545
1104	23	-0.010870887221706482
1104	24	0.02430370126221959
1104	25	-0.16459882835216888
1105	1	0.14159185011861908
1105	2	0.05340215540948279
1105	3	0.2169574627168842
1105	4	0.4239843883480461
1105	5	0.14670718424861917
1105	6	0.2028012604065026
1105	7	0.08012360068525481
1105	8	0.18167010180926013
1105	9	-1.6669525682252484
1105	10	-0.13425883873732403
1105	11	-0.051624102957902096
1105	12	0.11485545240690698
1105	13	0.4269305525933689
1105	14	0.2791577280934092
1105	15	0.22132190161556053
1105	16	0.437638345016822
1105	17	-1.0047975895579455
1105	18	0.18847959525639624
1105	19	0.3172092860144886
1105	20	0.17932262274680255
1105	21	0.09356748017554395
1105	22	0.1452122544221126
1105	23	0.3337259875673766
1105	24	0.5162369474369513
1105	25	-0.424044829597058
1106	1	-0.5436694376447591
1106	2	-0.40247114524688854
1106	3	-0.5423118986545248
1106	4	-0.42068553523957775
1106	5	-0.3624998807645383
1106	6	-0.13940846213561997
1106	7	-0.334417244848062
1106	8	-0.4641767851912064
1106	9	-2.986402905568447
1106	10	-0.8337654515799429
1106	11	-0.620837714139725
1106	12	-0.1823675443105799
1106	13	-0.6667294965163606
1106	14	-0.7042034197220417
1106	15	-0.47784932110369027
1106	16	-0.41537996405123323
1106	17	-0.6872235511780005
1106	18	-0.636320147207087
1106	19	-0.5371384610124608
1106	20	-0.6660611834327241
1106	21	-0.660966011020129
1106	22	-0.4278549758077119
1106	23	-0.14765043194774563
1106	24	-0.4044170228951671
1106	25	-0.7407325760044046
1107	1	-0.06947576092864693
1107	2	0.14364709454100932
1107	3	0.15595578797115195
1107	4	-0.6583033893987853
1107	5	0.003056868250858713
1107	6	-0.09264665233718716
1107	7	0.21676614116432802
1107	8	5.154112497152141E-4
1107	9	0.39690816268077356
1107	10	0.7000575852748696
1107	11	0.44833290826883143
1107	12	0.06282813818803845
1107	13	0.38137335148946505
1107	14	0.6212248462834751
1107	15	-0.027591572276932652
1107	16	-0.5086353760066231
1107	17	0.24504952783289013
1107	18	0.27358646776865214
1107	19	0.3216328913315323
1107	20	0.18653625999663603
1107	21	0.12853975544416585
1107	22	0.220033306084936
1107	23	0.3956407196383367
1107	24	0.12254939468488146
1107	25	-0.13225156805699356
1108	1	0.3729716273834036
1108	2	0.538372002047872
1108	3	0.17665038159807622
1108	4	0.6597377451836977
1108	5	0.48829377002911656
1108	6	0.43287349724311497
1108	7	0.3559394713151036
1108	8	0.47431101136110954
1108	9	0.9650905233443368
1108	10	0.7247940295601865
1108	11	0.6489694201835405
1108	12	0.39110915819838254
1108	13	0.45352993342048364
1108	14	0.44834821024894467
1108	15	0.7015328260203972
1108	16	0.45272039437170264
1108	17	0.2558384047871769
1108	18	0.7830346915025856
1108	19	0.53772072997576
1108	20	0.5095660567106464
1108	21	0.4599078582408396
1108	22	0.41433652444830216
1108	23	0.6363058745892047
1108	24	0.5219526647648394
1108	25	0.40905407700805085
1109	1	0.4189208531491424
1109	2	0.13146942450309496
1109	3	0.43583197472462487
1109	4	0.8922604382568551
1109	5	0.47032376857287517
1109	6	0.37442973237732957
1109	7	0.5441245108762871
1109	8	0.4038095793917833
1109	9	0.9506154978601344
1109	10	0.6121587205946
1109	11	0.4069811671861103
1109	12	0.39552641949637746
1109	13	0.17104850048033882
1109	14	0.8617650346749386
1109	15	0.5449384859398165
1109	16	0.5405899152687468
1109	17	0.8298590833950144
1109	18	0.5958107703337134
1109	19	0.20529026543520246
1109	20	0.31928403156623375
1109	21	0.42700654277071237
1109	22	0.3697949973379693
1109	23	0.6197144469886027
1109	24	0.4887795178302241
1109	25	0.29025401875845347
1110	1	0.4198021065697972
1110	2	0.2832449697722055
1110	3	0.40110028289327815
1110	4	0.922646674624481
1110	5	0.39348259311480405
1110	6	0.6126660299173494
1110	7	0.5299316926278479
1110	8	0.5223077586500651
1110	9	0.8272183741476127
1110	10	0.7479995548890699
1110	11	0.37054056521272116
1110	12	0.3166980433423774
1110	13	0.5186622620513006
1110	14	0.5231915932824194
1110	15	0.31137334688214935
1110	16	0.46015776095972427
1110	17	0.7837855054339755
1110	18	1.2239589322246047
1110	19	0.5118520474842256
1110	20	0.6715328769181141
1110	21	0.6484217223866509
1110	22	0.8903900443845316
1110	23	1.0036760470149915
1110	24	0.5464475048309652
1110	25	0.30491168530246426
1111	1	-0.23354392903073418
1111	2	-0.14331124596397515
1111	3	-0.48033793096832866
1111	4	0.18197391934685214
1111	5	0.0610971442523649
1111	6	-0.302157679978734
1111	7	-0.09535108322089059
1111	8	0.020692629142317287
1111	9	0.2841349227132781
1111	10	0.04797678925410054
1111	11	-0.06883003676399113
1111	12	-0.5159601829480351
1111	13	-0.4763969798735701
1111	14	-0.5027437974353575
1111	15	-0.44339564345168725
1111	16	-0.28113130722847757
1111	17	0.15860732844859626
1111	18	0.025728703973477707
1111	19	-0.09606013869354513
1111	20	-0.4663561129234921
1111	21	-0.4061706804543377
1111	22	-0.5246849078794185
1111	23	-0.1470591189177739
1111	24	-0.2942873944772444
1111	25	-0.30261081883534047
1112	1	0.37646238196835213
1112	2	0.17897430069013254
1112	3	0.26760230946722885
1112	4	0.8210947798042909
1112	5	0.6273510548523333
1112	6	0.3357878292725758
1112	7	0.40300072313372937
1112	8	0.3090937076090725
1112	9	-0.748811580472136
1112	10	0.16785103246495225
1112	11	-0.06599491209763642
1112	12	0.08138649547857568
1112	13	0.01849149685968854
1112	14	0.4814796698314012
1112	15	0.287122765558955
1112	16	0.24369195584754522
1112	17	0.21334221823771315
1112	18	0.17106020512277098
1112	19	0.2758507044520755
1112	20	0.3164519149761418
1112	21	0.4591869524771113
1112	22	0.3456269558338789
1112	23	0.11301387251508999
1112	24	0.055424943622469414
1112	25	0.2598960566797146
1113	1	0.11051739534303096
1113	2	0.11777221898418233
1113	3	0.24065881461517336
1113	4	0.34233446262161493
1113	5	0.3269450983820761
1113	6	0.5500445662909925
1113	7	0.29747768215071346
1113	8	0.07817105179477579
1113	9	0.20033809044945627
1113	10	0.3855107341652442
1113	11	0.32272878383702874
1113	12	0.09983906230846173
1113	13	0.31732143012824565
1113	14	-0.20455471329883324
1113	15	0.42465894953150496
1113	16	0.3040945789508953
1113	17	0.545827252427614
1113	18	0.470911210947925
1113	19	0.5314448542099939
1113	20	0.4838290588850995
1113	21	0.41146486003845695
1113	22	0.3883673131085388
1113	23	0.006962437931044008
1113	24	0.23275823177829835
1113	25	-0.0024802126783072254
1114	1	0.28205241014839544
1114	2	0.3533336485884675
1114	3	0.3221504955192458
1114	4	0.3462469474823777
1114	5	0.19762771094200104
1114	6	0.33416713537885556
1114	7	0.12279094342973451
1114	8	0.1364005787138325
1114	9	-0.23342090674604066
1114	10	0.055960329147538346
1114	11	-0.017246624979875946
1114	12	0.025488034682524585
1114	13	-0.06787965114699546
1114	14	0.2600491798446498
1114	15	0.036835729744353785
1114	16	0.26271507805059713
1114	17	0.5709747304498194
1114	18	0.503856949166952
1114	19	0.1129618631116287
1114	20	0.5077939496753814
1114	21	0.44694616106861673
1114	22	0.36208974588573284
1114	23	0.6502447622123029
1114	24	0.26989828639467106
1114	25	0.1457290978936001
1115	1	-0.12996247555070184
1115	2	-0.10137612860934908
1115	3	-0.028098404968890378
1115	4	0.40943384033434427
1115	5	0.14163235064042112
1115	6	-0.033915605909626834
1115	7	0.15340328696073868
1115	8	-0.07173822559722254
1115	9	0.13974617072725692
1115	10	-0.28896258419642606
1115	11	-0.20921414709093966
1115	12	-0.2238280931025045
1115	13	-0.06846927948782167
1115	14	-0.5073472727192652
1115	15	-0.43940031491274567
1115	16	-0.45288107486612716
1115	17	-0.061464494333897335
1115	18	0.1405492308353118
1115	19	-0.15691392829526052
1115	20	-0.22291022122017523
1115	21	-0.3188843932786867
1115	22	-0.7002465355893008
1115	23	-0.24625129887706265
1115	24	-0.17333790325299958
1115	25	0.11872218667615897
1116	1	0.7922100695726648
1116	2	0.6895620955716204
1116	3	0.6735875171080181
1116	4	1.1037974820110392
1116	5	0.4539578498687007
1116	6	0.7218838190403218
1116	7	0.5197687917500176
1116	8	0.21901005673517915
1116	9	0.5675120383984478
1116	10	0.5045657561014423
1116	11	0.2456713432113986
1116	12	0.3089990828385467
1116	13	0.4136040176990079
1116	14	0.762494123623398
1116	15	0.5719669741787499
1116	16	0.6583991686136415
1116	17	0.9513948787428816
1116	18	0.9600052975956643
1116	19	0.7253743706670122
1116	20	0.6535354841902006
1116	21	0.7209865702356524
1116	22	0.5772005042843134
1116	23	0.8320251094598348
1116	24	0.7079072651842466
1116	25	0.682226078048906
1201	1	-0.02387307527657967
1201	2	0.18120923576309877
1201	3	0.16165336796936458
1201	4	0.1126984759095393
1201	5	0.2387924035380138
1201	6	0.011825311086603563
1201	7	-0.02659719468792332
1201	8	0.007536955989557725
1201	9	0.4791409477710431
1201	10	0.5739539241020637
1201	11	0.4668635156146905
1201	12	0.35618369681910167
1201	13	0.3041498317028726
1201	14	0.8481261931512094
1201	15	0.29972411064269266
1201	16	0.38792880688912135
1201	17	0.7812478996379539
1201	18	0.7895383445284736
1201	19	0.5411342428533601
1201	20	0.3867145849424105
1201	21	0.2733748844786703
1201	22	0.2223713714797242
1201	23	0.3259923357693921
1201	24	-0.0921068092718258
1201	25	0.1027356393327139
1202	1	-0.2142670598552847
1202	2	-0.22357715990128924
1202	3	-0.2200529151052173
1202	4	0.14497103041698978
1202	5	-0.4009614192260713
1202	6	-0.3271627627931437
1202	7	-0.4126770973014615
1202	8	0.07179500884510048
1202	9	0.5397602980141087
1202	10	0.7090949138610725
1202	11	0.04740801862213573
1202	12	0.42193018901941315
1202	13	0.32137320126375357
1202	14	0.45055341245414127
1202	15	0.25407287856045
1202	16	0.17408791573922855
1202	17	0.7217169706657458
1202	18	0.6646715731394721
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2211	3	-0.16310598606526838
2211	4	-0.05409751142203234
2211	5	-0.11915643620774574
2211	6	-0.3661004008207698
2211	7	-0.565113807877708
2211	8	-0.35130194812445126
2211	9	-1.9998797047199268
2211	10	-0.3925464511776653
2211	11	-0.49295791810376244
2211	12	-0.49826656737552355
2211	13	-0.4651597804158385
2211	14	-0.5536537049986734
2211	15	-0.5440361576679038
2211	16	-0.3396400145711098
2211	17	-0.19194755826673315
2211	18	0.10767986245716088
2211	19	-0.5498264156098074
2211	20	-0.27386651640848214
2211	21	-0.43145694293185244
2211	22	-0.16825505167209087
2211	23	-2.2086596456038166
2211	24	-0.9105797954723727
2211	25	-0.6374543409867144
2212	1	0.22506092922879972
2212	2	-1.2338153769687743
2212	3	0.032492425732804975
2212	4	0.5213198848146234
2212	5	0.21473589159318607
2212	6	0.37968469868846455
2212	7	0.2541715012544472
2212	8	0.29217138044099045
2212	9	0.657231444753549
2212	10	0.7301160065894834
2212	11	0.43067028481123515
2212	12	0.35475866136007994
2212	13	0.49382416493423165
2212	14	0.7611111474021257
2212	15	0.5966676821308639
2212	16	0.5020428875966374
2212	17	0.8326715445471464
2212	18	0.8205053328756691
2212	19	0.5272832541724313
2212	20	0.6544621723384694
2212	21	0.4912873910837874
2212	22	0.5149430543231261
2212	23	0.8295282258603365
2212	24	0.6377405083575667
2212	25	0.6153568257498279
2213	1	0.35539745284720325
2213	2	0.11356249369406157
2213	3	0.16829502387563267
2213	4	0.47653398223310006
2213	5	0.2463933357236172
2213	6	0.2873219086535528
2213	7	0.2479112594894204
2213	8	0.22257250139475104
2213	9	0.42288561151917037
2213	10	0.5634662808906654
2213	11	0.20739149506130872
2213	12	0.08853784296884015
2213	13	-0.0993579663028541
2213	14	-1.2629988580601506
2213	15	-0.6449121027030174
2213	16	-0.08491890022961712
2213	17	0.21909090474854054
2213	18	0.09617256160248644
2213	19	-0.035615076271433566
2213	20	0.25687032300225354
2213	21	0.4268923973772729
2213	22	0.47981825322116833
2213	23	0.569318759500824
2213	24	0.3178383543214167
2213	25	0.3190801971607513
2214	1	0.013397116575493584
2214	2	0.024227849763303055
2214	3	-0.05830037289726775
2214	4	0.15563746707962878
2214	5	-0.041500513275607886
2214	6	-0.3209039994186327
2214	7	-0.05249300561832371
2214	8	-0.19258356008754163
2214	9	-0.4193918717642475
2214	10	-0.24300011318651826
2214	11	-0.11577762558817284
2214	12	-0.27088395445886704
2214	13	-0.43916606971195193
2214	14	-0.24651138233360825
2214	15	-0.6661267826259305
2214	16	-0.3117535672559555
2214	17	0.10436405160504592
2214	18	0.11092140174223886
2214	19	-0.09501322382080701
2214	20	-0.2281175471968666
2214	21	-0.1609308715162996
2214	22	-0.19811289976583168
2214	23	0.1446048214080733
2214	24	0.01599200092941544
2214	25	-0.06728398509851274
2215	1	0.5301000499868955
2215	2	0.49467417170846417
2215	3	0.6241350386782187
2215	4	0.6559320042189754
2215	5	0.23612037636936378
2215	6	0.3916554212178909
2215	7	0.5475335919778956
2215	8	0.46142544739370095
2215	9	0.8371089199158845
2215	10	0.6042118696553516
2215	11	0.3743776936633054
2215	12	0.3051191230079695
2215	13	0.11681294496313654
2215	14	0.45211910922209597
2215	15	0.37846792534495677
2215	16	0.3135336064549037
2215	17	0.6682321989307383
2215	18	0.8309424299089159
2215	19	0.4089059052060904
2215	20	-0.33256487949366687
2215	21	0.10413827330850722
2215	22	-0.6105416530168952
2215	23	0.37471419286582375
2215	24	0.5528385970618714
2215	25	0.6602807639826196
2216	1	0.6509754481093388
2216	2	0.032244837886683086
2216	3	0.40890350969241496
2216	4	0.8299970944600616
2216	5	0.5993778760092496
2216	6	0.4211462690697658
2216	7	0.48849765737045203
2216	8	0.32252317167156264
2216	9	0.7970165892968666
2216	10	0.4465971721572828
2216	11	0.2032877406644289
2216	12	-0.1747733594799783
2216	13	0.21899637204382572
2216	14	0.25585963742010165
2216	15	0.12295289583365587
2216	16	0.2398869560184167
2216	17	0.317614142177284
2216	18	0.7489732198229717
2216	19	0.37502081699229706
2216	20	-0.7027142765526762
2216	21	-0.656827321443302
2216	22	-0.8373322926269395
2216	23	-1.2644032601463948
2216	24	-1.1991233396759995
2216	25	-0.9366806125829683
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1218.0
3	156.0
4	148.0
5	88.0
6	112.0
7	183.0
8	94.0
9	195.0
10	401.0
11	935.0
12	1891.0
13	3349.0
14	4916.0
15	6677.0
16	8385.0
17	9129.0
18	9035.0
19	8343.0
20	7684.0
21	7160.0
22	6578.0
23	6312.0
24	5924.0
25	5709.0
26	5688.0
27	5991.0
28	6631.0
29	7470.0
30	9167.0
31	11601.0
32	15216.0
33	20525.0
34	28603.0
35	41409.0
36	64881.0
37	111602.0
38	173281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4332776719559	22.649593448180376	11.98039824456882	19.936730635294907
2	15.55204856454264	26.613187854830738	35.63013233568963	22.204631244936994
3	19.903136298663078	32.78903556714033	27.265113932565377	20.042714201631213
4	12.943042605765884	24.423504239718618	30.999651806485147	31.63380134803035
5	11.53975548542938	44.544680765167776	29.74798312486122	14.167580624541621
6	26.08759259103559	39.915585562104305	19.125251183399612	14.871570663460487
7	22.964928565663087	40.71101380256679	18.10981515860844	18.214242473161676
8	22.183874988016488	38.79966429351108	20.396692046480887	18.619768671991547
9	25.48587976315201	24.303321488691534	17.407419241460843	32.80337950669561
10	16.28563402362733	33.501323002753665	25.80873064067649	24.404312332942517
11	28.134481014019926	33.00187078002955	17.996971118047398	20.866677087903128
12	22.509908907001734	33.825485523830984	23.27134345776712	20.393262111400162
13	27.059050981381077	28.082545286428783	25.302904215525572	19.555499516664565
14	23.579406458641927	31.237001255949785	23.09568925078476	22.087903034623526
15	27.252605305471644	30.814422863447682	22.464326320817552	19.468645510263123
16	21.486161170584754	32.58429044058314	24.395139598687397	21.53440879014471
17	19.628573158507294	34.99508890428745	23.39783911788648	21.97849881931877
18	19.90029340305509	29.621602535540443	30.049348880612698	20.428755180791768
19	21.196802830567847	34.54177324178384	24.197554531985986	20.06386939566233
20	21.849763648222485	30.044580719343188	27.58036969049012	20.525285941944205
21	23.842949564187897	32.488728417505136	23.446741457393333	20.221580560913633
22	22.032234422382547	31.089676781667936	23.319526905724064	23.55856189022545
23	24.023697478991597	29.19109243697479	23.954621848739496	22.830588235294115
24	21.351329540410767	30.69358700184266	23.460806466798477	24.494276990948098
25	22.841618065631152	32.03112259565676	24.493913172127524	20.63334616658457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18915.0
1	18915.0
2	12129.5
3	5344.0
4	5344.0
5	5344.0
6	5764.0
7	6184.0
8	6184.0
9	6184.0
10	6112.5
11	6041.0
12	6041.0
13	6041.0
14	6498.5
15	6956.0
16	6956.0
17	6956.0
18	8706.0
19	10456.0
20	10456.0
21	10456.0
22	13084.0
23	15712.0
24	15712.0
25	15712.0
26	19725.5
27	23739.0
28	23739.0
29	23739.0
30	26404.0
31	29069.0
32	29069.0
33	29069.0
34	31936.0
35	34803.0
36	34803.0
37	34803.0
38	38437.5
39	42072.0
40	42072.0
41	42072.0
42	71342.5
43	100613.0
44	100613.0
45	100613.0
46	94703.5
47	88794.0
48	88794.0
49	88794.0
50	80504.5
51	72215.0
52	72215.0
53	72215.0
54	59515.0
55	46815.0
56	46815.0
57	46815.0
58	40183.0
59	33551.0
60	33551.0
61	33551.0
62	31539.5
63	29528.0
64	29528.0
65	29528.0
66	20898.5
67	12269.0
68	12269.0
69	12269.0
70	10344.5
71	8420.0
72	8420.0
73	8420.0
74	5921.5
75	3423.0
76	3423.0
77	3423.0
78	2343.0
79	1263.0
80	1263.0
81	1263.0
82	806.5
83	350.0
84	350.0
85	350.0
86	228.5
87	107.0
88	107.0
89	107.0
90	70.5
91	34.0
92	34.0
93	34.0
94	21.5
95	9.0
96	9.0
97	9.0
98	7.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1377606684911855
2	0.2823926112015177
3	0.3413850142537042
4	0.36702659853491026
5	0.3692052952385421
6	0.33551929235931066
7	0.33769798906294257
8	0.35529515474612317
9	0.34255815863258293
10	0.36970807140091877
11	0.2929509106114261
12	0.3385359493335702
13	0.31205640478173646
14	0.3214415598127662
15	0.357138667341504
16	0.30820178753684935
17	0.3544571944754955
18	0.3254637691117789
19	0.3412174221995787
20	0.30350921002133446
21	0.34356371095733607
22	0.300827737155326
23	0.28272779530976877
24	0.31758694256787895
25	0.31473787764774497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	596687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.023166419145344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70141711275225	19.943818242541184
2	7.367355251618651	3.6870911306043586
3	3.0123834483829106	2.2613811704149347
4	1.749769707899239	1.7513911438376792
5	1.17355874590171	1.4683077900670995
6	0.859746591466873	1.2908149221941116
7	0.6874527871160458	1.2041571848117076
8	0.5422073120606935	1.0854195042697763
9	0.4512327735325732	1.0162145507290288
>10	3.681702481105452	18.650552032761517
>50	0.4081012951437619	7.038027157148612
>100	0.2938060395021811	14.463167678751947
>500	0.04370112715707499	7.532980446251523
>1k	0.02487602622787346	11.659497874911013
>5k	0.0013446500663715382	2.0916907693461937
>10k+	0.0013446500663715382	4.8554884013593265
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	17127	2.8703491110079487	No Hit
GTACATAAGCAGTGGTATCAACGCA	11734	1.9665251631089669	No Hit
GCTTATGTACTCTGCGTTGATACCA	7115	1.1924174651031445	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5318	0.8912545438395675	No Hit
CTTATGTACTCTGCGTTGATACCAC	4936	0.8272343791636151	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3746	0.6277998347542346	No Hit
GAGTACATAAGCAGTGGTATCAACG	3346	0.5607630131040227	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3325	0.5572435799673866	No Hit
TATGTACTCTGCGTTGATACCACTG	3011	0.5046196749719702	No Hit
GTGGTATCAACGCAGAGTACATGGG	2847	0.47713457809538334	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2475	0.4147903339606862	No Hit
ATACAGGGTGACAGCCCCGTACACA	2437	0.4084218359039161	No Hit
GCGTTGATACCACTGCTTATGTACT	2410	0.4038968504425268	No Hit
CCCATGTACTCTGCGTTGATACCAC	2400	0.4022209299012715	No Hit
CATAAGCAGTGGTATCAACGCAGAG	2231	0.373897872754057	No Hit
GGATACCACGTGTCCCGCCCTACTC	2023	0.33903872549594677	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1962	0.32881561019428945	No Hit
CTGCTTATGTACTCTGCGTTGATAC	1899	0.3182573107843811	No Hit
CATGTACTCTGCGTTGATACCACTG	1880	0.31507306175599603	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1782	0.2986490404516941	No Hit
GTTAATGATAGTGTGTCGAAACACA	1707	0.2860796363922794	No Hit
GTATCAACGCAGAGTACATAAGCAG	1609	0.2696556150879775	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1608	0.26948802303385194	No Hit
ATCATTAACTGAATCCATAGGTTAA	1565	0.26228156470645414	No Hit
GGTATCAACGCAGAGTACATAAGCA	1523	0.25524269843318187	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1449	0.24284088642789267	No Hit
TATCAACGCAGAGTACATAAGCAGT	1402	0.23496405988399277	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1356	0.22725482539421843	No Hit
GGTATCAACGCAGAGTACATGGGGG	1213	0.20328916165426764	No Hit
CCCATATTCAGACAGGATACCACGT	1195	0.2002725046800081	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1185	0.19859658413875278	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1162	0.19474196689386564	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1119	0.18753550856646783	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	1112	0.18636236418758914	No Hit
GTGGTATCAACGCAGAGTACATAAG	1103	0.18485403570045938	No Hit
GTATCAACGCAGAGTACATGGGGGT	1099	0.18418366748395726	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1080	0.1809994184555722	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1060	0.17764757737306158	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	1036	0.17362536807404888	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1006	0.168597606450283	No Hit
GTACATGATAAGCAGTGGTATCAAC	1005	0.16843001439615746	No Hit
ACATAAGCAGTGGTATCAACGCAGA	994	0.1665865018007766	No Hit
ATCATGTACTCTGCGTTGATACCAC	979	0.16407262098889366	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	975	0.16340225277239157	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	951	0.15938004347337886	No Hit
TATTCAGACAGGATACCACGTGTCC	940	0.15753653087799802	No Hit
CACACACACTGATTCAGGCTCTGGG	933	0.1563633864991193	No Hit
GGGTTTCCCCATTCGGAAATCGCCG	887	0.14865415200934493	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	853	0.1429560221690769	No Hit
GTACTCTGCGTTGATACCACTGCTT	853	0.1429560221690769	No Hit
GCAGTGGTATCAACGCAGAGTACAT	851	0.14262083806082587	No Hit
GTTATAACCGGCGATTTCCGAATGG	841	0.14094491751957058	No Hit
GTACATGGGGGTTAAGCGACTAAGC	832	0.1394365890324408	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	817	0.13692270822055785	No Hit
GATACCACTGCTTATGTACTCTGCG	810	0.13574956384167913	No Hit
CCACTGCTTATGTACTCTGCGTTGA	803	0.1345764194628004	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	788	0.1320625386509175	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	786	0.13172735454266643	No Hit
TCATTAACCTATGGATTCAGTTAAT	778	0.1303866181096622	No Hit
AGCGTACACGGTGGATGCCCTGGCA	767	0.12854310551428136	No Hit
TTCCAGACGCTTCCACTAACACACA	767	0.12854310551428136	No Hit
GTGGATGCCCTGGCAGTCAGAGGCG	765	0.1282079214060303	No Hit
GTACTTAGATGTTTCAGTTCCCCCG	758	0.12703477702715157	No Hit
GTGCTAATCTGCGATAAGCGTCGGT	752	0.1260292247023984	No Hit
TCACGGTACTGGTTCACTATCGGTC	740	0.12401812005289205	No Hit
ATACCACTGCTTATGTACTCTGCGT	739	0.12385052799876652	No Hit
CGGTACTGGTTCACTATCGGTCAGT	734	0.12301256772813889	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	732	0.12267738361988782	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	724	0.12133664718688357	No Hit
GTTGATACCACTGCTTATGTACTCT	714	0.11966072664562827	No Hit
CCTATGGATTCAGTTAATGATAGTG	691	0.1158061094007411	No Hit
AGCTCACAGCATGTGCATTTTTGTG	689	0.11547092529249003	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	685	0.11480055707598791	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC	660	0.11061075572284967	No Hit
CTACTGGGGGAATCTCGGTTGATTT	659	0.11044316366872414	No Hit
GAATCAGTGTGTGTGTTAGTGGAAG	653	0.10943761134397097	No Hit
ACGCAGAGTACATGGGGGTTAAGCG	629	0.10541540204495824	No Hit
GCCTGAATCAGTGTGTGTGTTAGTG	625	0.10474503382845612	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	624	0.1045774417743306	No Hit
GCCCTACTCATCGAGCTCACAGCAT	623	0.10440984972020506	No Hit
GATTCAGGCTCTGGGCTGCTCCCCG	617	0.10340429739545189	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	609	0.10206356096244765	No Hit
ATGATAGTGTGTCGAAACACACTGG	609	0.10206356096244765	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	608	0.10189596890832212	No Hit
GGTATCAACGCAGAGTACATGGGGA	603	0.10105800863769447	No Hit
TATCAACGCAGAGTACATGGGGGTT	598	0.10022004836706681	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6759205412552981E-4	0.0
2	0.0	0.0	0.0	1.6759205412552981E-4	0.0
3	0.0	0.0	0.0	1.6759205412552981E-4	0.0
4	0.0	0.0	0.0	1.6759205412552981E-4	0.0
5	6.703682165021193E-4	0.0	0.0	1.6759205412552981E-4	0.0
6	6.703682165021193E-4	0.0	0.0	1.6759205412552981E-4	0.0
7	6.703682165021193E-4	0.0	0.0	1.6759205412552981E-4	0.0
8	8.37960270627649E-4	0.0	0.0	1.6759205412552981E-4	0.0
9	8.37960270627649E-4	0.0	0.0	1.6759205412552981E-4	0.0
10	8.37960270627649E-4	0.0	0.0	1.6759205412552981E-4	0.0
11	8.37960270627649E-4	0.0	0.0	1.6759205412552981E-4	0.0
12	8.37960270627649E-4	0.0	0.0	1.6759205412552981E-4	0.0
13	8.37960270627649E-4	0.0	0.0	3.3518410825105963E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCCAA	25	0.0060308785	18.998318	11
GTCGCCT	35	0.0021693632	16.284271	11
TGTAGTT	35	0.0021693632	16.284271	15
GTACAAA	75	5.7947545E-8	15.206327	1
GGGTCCT	45	6.758181E-4	14.776469	7
CTCGTGT	40	0.0052741864	14.249936	14
CCAGCCG	40	0.0052741864	14.249936	14
TCTCGTG	40	0.0052741864	14.249936	13
TCGTGTC	40	0.0052771308	14.248738	15
GTTCCTT	40	0.0052771308	14.248738	6
CGGTCTC	40	0.0052771308	14.248738	10
TCAGCAA	55	1.9584197E-4	13.816958	10
CCTGACT	55	1.9640164E-4	13.812313	18
TGGGTCC	50	0.0014986488	13.298821	6
CGAATGG	150	0.0	13.294351	19
GTCTTCG	60	4.0711023E-4	12.67194	1
GGGCAAG	60	4.0884773E-4	12.665546	7
TCGGTCT	60	4.0884773E-4	12.665546	15
CGGTCTT	60	4.0913795E-4	12.664481	16
TACAGGG	465	0.0	12.465455	2
>>END_MODULE