FastQCFastQC Report
Wed 25 May 2016
SRR1295172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596687
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT164622.758900395014472No Hit
GTACATAAGCAGTGGTATCAACGCA125802.108308040899165No Hit
GGTATCAACGCAGAGTACTTTTTTT114641.9212753084950736No Hit
TATCAACGCAGAGTACTTTTTTTTT99811.672736292226913No Hit
GCTTATGTACTCTGCGTTGATACCA74921.2555996695084692No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56740.9509173151082561No Hit
CTTATGTACTCTGCGTTGATACCAC56070.9396886474818456No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51630.8652777754501104No Hit
GAGTACATAAGCAGTGGTATCAACG36110.6051749074472881No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30460.5104853968663637No Hit
GTGGTATCAACGCAGAGTACATGGG29890.5009326497812085No Hit
CCCATGTACTCTGCGTTGATACCAC26950.4516605858683028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA26900.45082262559767516No Hit
GCGTTGATACCACTGCTTATGTACT26670.446968008352788No Hit
TATGTACTCTGCGTTGATACCACTG26550.44495690370328167No Hit
CATAAGCAGTGGTATCAACGCAGAG26270.44026432618776673No Hit
ATACAGGGTGACAGCCCCGTACACA26240.43976155002539025No Hit
GTACTGGTTCACTATCGGTCAGTCA26130.43791803743000934No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22480.376746937674191No Hit
ACGCAGAGTACATAAGCAGTGGTAT22290.37356268864580594No Hit
GGATACCACGTGTCCCGCCCTACTC21580.3616636528028933No Hit
CTGCTTATGTACTCTGCGTTGATAC21290.35680348323325295No Hit
ACTCTGCGTTGATACCACTGCTTAT20220.3388711334418213No Hit
CATGTACTCTGCGTTGATACCACTG19590.3283128340319129No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18900.31674898229725135No Hit
GTTAATGATAGTGTGTCGAAACACA16940.28390093968864744No Hit
ATCATTAACTGAATCCATAGGTTAA16700.2798787303896348No Hit
GTATCAACGCAGAGTACATAAGCAG16700.2798787303896348No Hit
GTGGTATCAACGCAGAGTACTTTTT16290.27300745617048805No Hit
ATCAACGCAGAGTACTTTTTTTTTT16080.26948802303385194No Hit
TATCAACGCAGAGTACATAAGCAGT15690.26295193292295627No Hit
GGTATCAACGCAGAGTACATAAGCA14690.2461927275104033No Hit
CCCATATTCAGACAGGATACCACGT14080.23596961220874596No Hit
GGTATCAACGCAGAGTACATGGGGG13840.23194740290973323No Hit
AAGCAGTGGTATCAACGCAGAGTAC12760.21384746106417601No Hit
ACTTAGATGTTTCAGTTCCCCCGGT11730.19658547948924648No Hit
CACACACACTGATTCAGGCTCTGGG11380.1907197575948529No Hit
TCTAAGTACCCCGAGGAAAAGAAAT11380.1907197575948529No Hit
GGTTAATGAGGCGAACCGGGGGAAC11290.18921142910772315No Hit
GTGGTATCAACGCAGAGTACATAAG11250.18854106089122102No Hit
GTACATGGGAAGCAGTGGTATCAAC11200.18770310062059337No Hit
GCCCAGAGCCTGAATCAGTGTGTGT11020.18468644364633385No Hit
ACATAAGCAGTGGTATCAACGCAGA10980.18401607542983173No Hit
GTATCAACGCAGAGTACATGGGGGT10910.182842931050953No Hit
ATCATGTACTCTGCGTTGATACCAC10890.18250774694270194No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10790.18083182640144665No Hit
GTACATGATAAGCAGTGGTATCAAC10420.17463092039880207No Hit
GTATTTAGCCTTGGAGGATGGTCCC10220.17127907931629147No Hit
GCCTTGGAGGATGGTCCCCCCATAT9910.16608372563840001No Hit
AGCGTACACGGTGGATGCCCTGGCA9620.16122355606875965No Hit
GTCAGGAGTATTTAGCCTTGGAGGA9420.15787171498624908No Hit
GTTCACTATCGGTCAGTCAGGAGTA9400.15753653087799802No Hit
GTTATAACCGGCGATTTCCGAATGG9300.15586061033674273No Hit
CTTATACACATCTCCGAGCCCACGA9240.15485505801198954No Hit
CATCTAAGTACCCCGAGGAAAAGAA9240.15485505801198954No Hit
GATACCACTGCTTATGTACTCTGCG9200.1541846897954874No Hit
CTATCGGTCAGTCAGGAGTATTTAG9140.15317913747073425No Hit
CGGTACTGGTTCACTATCGGTCAGT9110.15267636130835766No Hit
CCCCAGTAGCGGCGAGCGAACGGGG8980.15049766460472577No Hit
TATTCAGACAGGATACCACGTGTCC8890.148989336117596No Hit
GGGTTTCCCCATTCGGAAATCGCCG8770.14697823146808964No Hit
TCACGGTACTGGTTCACTATCGGTC8610.14429675860208116No Hit
GCAGTGGTATCAACGCAGAGTACAT8610.14429675860208116No Hit
CCACTGCTTATGTACTCTGCGTTGA8440.14144769368194715No Hit
TCATTAACCTATGGATTCAGTTAAT8230.13792826054531104No Hit
GTACTCTGCGTTGATACCACTGCTT8230.13792826054531104No Hit
GTACATGGGGGTTAAGCGACTAAGC8190.13725789232880892No Hit
GTGGATGCCCTGGCAGTCAGAGGCG8040.13474401151692597No Hit
GCCTGAATCAGTGTGTGTGTTAGTG7900.13239772275916853No Hit
CTACTGGGGGAATCTCGGTTGATTT7810.13088939427203877No Hit
GTACTTAGATGTTTCAGTTCCCCCG7750.1298838419472856No Hit
ATACCACTGCTTATGTACTCTGCGT7540.12636440881064948No Hit
ACGCAGAGTACATGGGGGTTAAGCG7380.123682935944641No Hit
TTCCAGACGCTTCCACTAACACACA7230.12116905513275805No Hit
CCTATGGATTCAGTTAATGATAGTG7110.11915795048325169No Hit
GATTCAGGCTCTGGGCTGCTCCCCG7020.11764962199612192No Hit
GCCCTACTCATCGAGCTCACAGCAT6990.11714684583374532No Hit
CAACGCAGAGTACTTTTTTTTTTTT6970.11681166172549427No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC6900.11563851734661557No Hit
GGTATCAACGCAGAGTACATGGGGA6890.11547092529249003No Hit
AGCTCACAGCATGTGCATTTTTGTG6850.11480055707598791No Hit
GTCCCGCCCTACTCATCGAGCTCAC6820.11429778091361133No Hit
GTGCTAATCTGCGATAAGCGTCGGT6810.1141301888594858No Hit
GAATCAGTGTGTGTGTTAGTGGAAG6810.1141301888594858No Hit
CACTGATTCAGGCTCTGGGCTGCTC6800.11396259680536025No Hit
GTTGATACCACTGCTTATGTACTCT6750.11312463653473262No Hit
AACGCAGAGTACTTTTTTTTTTTTT6430.10776169080271567No Hit
CAGTAGCGGCGAGCGAACGGGGAGC6420.10759409874859013No Hit
TATCAACGCAGAGTACATGGGGGTT6330.10608577026146035No Hit
GGGTGACAGCCCCGTACACAAAAAT6070.10172837685419658No Hit
ATGATAGTGTGTCGAAACACACTGG6050.10139319274594552No Hit
GGTTCACTATCGGTCAGTCAGGAGT6030.10105800863769447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCG250.00602566719.0011759
CTTATCT250.00602566719.0011759
GTGAGGT250.00602566719.0011759
CTATAGT307.716253E-418.9995813
TCTATAG307.716253E-418.9995812
GTACAAA504.5428624E-617.1039241
CTTATAC1050.016.2894521
TCCAAAA350.002167197216.286722
CGAACTT350.002170840716.28262515
TGGGGTC508.695021E-515.2009396
CTGACCG456.7634124E-414.77497616
TCGCCTT400.00526603514.253275
ATATTAC400.00526603514.253271
GTCGCCT400.005271896714.2508811
ATGTCTA400.005271896714.250889
TTATACA1203.6379788E-1214.250882
CGACCTC400.0052748314.24968613
GCATAAT400.005280714.24729816
AACTTCT400.005280714.24729817
ACTTCTG400.005280714.24729818
Produced by FastQC (version 0.11.5)