FastQCFastQC Report
Wed 25 May 2016
SRR1295171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5955208
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG81230.13640161687047705No Hit
GGTATCAACGCAGAGTACATGGGGG71140.1194584639193123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN68460.11495820129204554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18150.011.9954451
TCGACCG1157.107883E-811.5627959
TGGTATC17450.011.3306752
GGTATCA39700.011.0399711
GTACCGT1856.7338988E-99.2414636
TAGACAG7550.09.0649655
GTATTAG6300.08.9036681
CGCCTAA1651.0541753E-68.63484614
GTCTTAG5200.08.410311
TGCGTTA1252.750899E-48.35853213
AGGACCG2201.4517354E-88.2094025
CTAGACA4650.08.17715554
GTCGTAA1401.0308322E-48.149121
TCTAGAC4900.08.1478143
GTATCAA71500.08.1111291
ACAGTCG1909.2035407E-77.99845938
CCGCCTA1803.6593556E-67.91527613
GTGTAGA10150.07.86811641
AGGACTG12000.07.8421395
TAATACG1701.4415757E-57.8284244
Produced by FastQC (version 0.11.5)