##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295171_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5955208
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.011623271596896	31.0	30.0	33.0	26.0	34.0
2	29.99905091476234	31.0	30.0	34.0	25.0	34.0
3	30.23249045205474	31.0	30.0	34.0	26.0	34.0
4	33.56199011016911	35.0	35.0	37.0	27.0	37.0
5	33.87062500587721	35.0	35.0	37.0	28.0	37.0
6	33.70910890098213	35.0	35.0	37.0	28.0	37.0
7	33.71750474542619	36.0	35.0	37.0	28.0	37.0
8	33.78826835267551	36.0	35.0	37.0	28.0	37.0
9	34.9104132047109	39.0	35.0	39.0	27.0	39.0
10	34.92165345022374	38.0	35.0	39.0	27.0	39.0
11	35.336027893568115	39.0	35.0	39.0	27.0	39.0
12	35.33555167174681	39.0	35.0	39.0	27.0	39.0
13	35.25848366673339	39.0	35.0	39.0	27.0	39.0
14	35.89209109068902	39.0	35.0	41.0	27.0	41.0
15	36.23611668979488	39.0	36.0	41.0	27.0	41.0
16	36.20929277365291	39.0	36.0	41.0	27.0	41.0
17	35.862656182622004	39.0	35.0	41.0	27.0	41.0
18	35.75420035706561	39.0	35.0	41.0	26.0	41.0
19	36.17036449440557	39.0	36.0	41.0	27.0	41.0
20	36.20409580991965	39.0	36.0	41.0	27.0	41.0
21	36.23774652371504	39.0	36.0	41.0	27.0	41.0
22	36.14760609537064	39.0	36.0	41.0	27.0	41.0
23	35.83900377619052	39.0	35.0	41.0	26.0	41.0
24	36.13733693264786	39.0	35.0	41.0	27.0	41.0
25	34.74991066642844	38.0	33.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1678014785713806
1101	2	0.02232540878632605
1101	3	-0.07060569610147382
1101	4	-0.03333839330262123
1101	5	-0.20457311113756305
1101	6	-0.43975894587300246
1101	7	-0.40995453175104757
1101	8	-0.2687093391201145
1101	9	-0.060806258723772544
1101	10	-0.04709916354187982
1101	11	-0.03747368218166258
1101	12	-0.0863846334072349
1101	13	-0.05490417433144046
1101	14	0.2360017628092308
1101	15	0.019448642493195223
1101	16	-0.01987431682141505
1101	17	-0.030331300206434264
1101	18	0.17851317496552355
1101	19	-0.06883142451047064
1101	20	-0.008009782128432619
1101	21	-0.03176670260757675
1101	22	-0.03617864483184974
1101	23	0.03637316403578694
1101	24	-0.12116758557700535
1101	25	-0.1682687284347253
1102	1	-0.20070591534757298
1102	2	-0.007043333447640521
1102	3	-0.13194156652069466
1102	4	-0.10865522230329816
1102	5	-0.4004543606992854
1102	6	-0.5620384198071875
1102	7	-0.5722643606582594
1102	8	-0.48626494563795575
1102	9	-0.28365065773258635
1102	10	0.10829695304396836
1102	11	-0.04987674524284813
1102	12	-0.15480111123738993
1102	13	-0.24246941974833902
1102	14	-0.00889189707091731
1102	15	-0.08840114641688501
1102	16	-0.10949366132118854
1102	17	-0.27333940020891845
1102	18	0.04995766915519084
1102	19	-0.16579803549338834
1102	20	-0.07600184820765321
1102	21	-0.0568316468021095
1102	22	-0.23849491528869748
1102	23	-0.12920463752262634
1102	24	-0.1694255138312215
1102	25	-0.370597297223604
1103	1	-0.4538758216455001
1103	2	-0.28352073552362356
1103	3	-0.46942246957764766
1103	4	-0.36505501543091157
1103	5	-0.5195918818231675
1103	6	-0.8139870627920374
1103	7	-0.6894192676190514
1103	8	-0.5290989744637216
1103	9	-0.051069115250712116
1103	10	0.03658463444341464
1103	11	-0.20002619446538006
1103	12	-0.3414904346504599
1103	13	-0.2561040935886538
1103	14	-0.3631817696168085
1103	15	-0.3413531100163425
1103	16	-0.3251884458707224
1103	17	-0.24371670705232873
1103	18	0.08669348483226713
1103	19	-0.21364189276155088
1103	20	-0.27110033979149506
1103	21	-0.29087979878526227
1103	22	-0.22923290607964475
1103	23	-2.5103735431792913
1103	24	-1.1956430510974627
1103	25	-0.6297103843678755
1104	1	-0.2713650769158953
1104	2	-0.13418166255255315
1104	3	-0.311190027123164
1104	4	-0.593509693681888
1104	5	-0.3805229704564823
1104	6	-0.5808206093837285
1104	7	-0.5494057482964081
1104	8	-0.4682281621037703
1104	9	-2.6325301702990203
1104	10	-0.7887854505770733
1104	11	-0.5432752440808315
1104	12	-0.4961700151838855
1104	13	-0.3244339283041242
1104	14	-0.12493483830527907
1104	15	-0.3055613536645936
1104	16	-0.22105324847136387
1104	17	0.057212454498184684
1104	18	0.23153454411696117
1104	19	-0.21545806064885653
1104	20	-0.27604782856088406
1104	21	-0.3753274326407734
1104	22	-0.2906278729753069
1104	23	-0.20288004774963042
1104	24	-0.24049274030375756
1104	25	-0.26969029849172443
1105	1	-0.2763521141490415
1105	2	-0.21227792041159788
1105	3	-0.31821902371479993
1105	4	-0.09941381235101687
1105	5	-0.19400377732498697
1105	6	-0.3650439219687911
1105	7	-0.4103720921686076
1105	8	-0.31523880294687956
1105	9	-2.2145363594808956
1105	10	-0.6163908490497789
1105	11	-0.5077720886962851
1105	12	-0.3144558706676648
1105	13	-0.20543972919131903
1105	14	-0.24125835445088484
1105	15	-0.25238373042025586
1105	16	-0.2808315675083932
1105	17	-1.6218771317522638
1105	18	-0.36306917958980023
1105	19	-0.38706587517735613
1105	20	-0.32656841798436886
1105	21	-0.24621450948782098
1105	22	-0.27199090496843326
1105	23	-0.1997543147943972
1105	24	-0.24951129608287914
1105	25	-0.8134155741221107
1106	1	-0.4142305823637109
1106	2	-0.6346089412570848
1106	3	-0.5361781601102145
1106	4	-0.48454348128053226
1106	5	-0.4007895019527723
1106	6	-0.6329013337198646
1106	7	-0.588025599579737
1106	8	-0.5131808128669917
1106	9	-3.3480340076931654
1106	10	-0.9523092873138026
1106	11	-0.8345002286535106
1106	12	-0.579706996919569
1106	13	-0.5970422956805166
1106	14	-0.9245544149478846
1106	15	-0.7178251508421312
1106	16	-0.7021927499798224
1106	17	-0.6435848978228123
1106	18	-0.28489980950487137
1106	19	-0.44805391560245766
1106	20	-0.5318983922119145
1106	21	-0.4210326337664583
1106	22	-0.40615946423472593
1106	23	-0.24986679519616928
1106	24	-0.5653252380266096
1106	25	-0.8245579596663788
1107	1	-0.3156809637292213
1107	2	-0.10668597505534194
1107	3	-0.16883802553622118
1107	4	-1.5567698657212503
1107	5	-0.49521494245522035
1107	6	-0.37016113906222614
1107	7	-0.24683084312939485
1107	8	-0.2078193778008881
1107	9	0.11042024588868316
1107	10	0.3305005129109304
1107	11	0.004684523570524846
1107	12	-0.656666340005053
1107	13	-0.215420676275194
1107	14	0.10745115120512594
1107	15	-0.7112320359908466
1107	16	-0.9793201134116885
1107	17	-0.15098595960340333
1107	18	-0.23182015692770364
1107	19	-0.0664972231559986
1107	20	-0.446231777841696
1107	21	-0.1697481219107857
1107	22	-0.13030391905368788
1107	23	0.034468905043588904
1107	24	-0.2217048644408024
1107	25	-0.39836098942105735
1108	1	0.2880820103949375
1108	2	0.3518940940143267
1108	3	0.35011687862670016
1108	4	0.7717161948158875
1108	5	0.5763772748552185
1108	6	0.48542574317183096
1108	7	0.4518056042343943
1108	8	0.4422049763520164
1108	9	0.8123205399099405
1108	10	0.8065474602083569
1108	11	0.6264091434515748
1108	12	0.5588900965926982
1108	13	0.44161151553015543
1108	14	0.4781626124941809
1108	15	0.5101246414756133
1108	16	0.5879209802513046
1108	17	0.6222199674344822
1108	18	0.6813062608018043
1108	19	0.5020266151484236
1108	20	0.4830029771378648
1108	21	0.44961560367465836
1108	22	0.5190872065884804
1108	23	0.6398699684468383
1108	24	0.4628751507403024
1108	25	0.548292407788999
1109	1	0.17394006710366128
1109	2	0.004915626436904574
1109	3	0.2106330896446167
1109	4	0.5019336259438916
1109	5	0.4043864544628235
1109	6	0.3234329362562818
1109	7	0.2992487876481036
1109	8	0.2426957149006057
1109	9	0.6937370491468755
1109	10	0.3512123107510803
1109	11	0.24241687107924292
1109	12	0.22578807148488522
1109	13	0.1728942209051425
1109	14	0.5111427948666503
1109	15	0.27144674688861414
1109	16	0.29928862729006767
1109	17	0.5649245978160167
1109	18	0.34756871795515565
1109	19	0.13663986795642558
1109	20	0.09264423042939995
1109	21	0.18194067798350488
1109	22	0.1700767810843331
1109	23	0.42414514130275904
1109	24	0.22795800144859157
1109	25	0.22992629521291974
1110	1	0.24883014666600545
1110	2	0.22220511131837029
1110	3	0.33876822148975805
1110	4	0.5133926801937747
1110	5	0.48116148558862903
1110	6	0.4857234316479335
1110	7	0.46265457000072985
1110	8	0.4854653499356516
1110	9	1.0547119278517627
1110	10	1.025651125631157
1110	11	0.6146486694577504
1110	12	0.5277843630160959
1110	13	0.5574651008379732
1110	14	0.678204115301547
1110	15	0.5017563081393348
1110	16	0.530699190717641
1110	17	0.9075717297095736
1110	18	0.9642801170382569
1110	19	0.5326640969026641
1110	20	0.5713426291007622
1110	21	0.5529207409695331
1110	22	0.5365038918127638
1110	23	0.7013265501323787
1110	24	0.5353026159434293
1110	25	0.5404901382029408
1111	1	0.18075493161386547
1111	2	0.1495506792625676
1111	3	0.2101356199115294
1111	4	0.500541902590399
1111	5	0.3742667347777484
1111	6	0.3693181563017731
1111	7	0.21887339736287004
1111	8	0.20528555635727486
1111	9	0.5966361858003211
1111	10	0.45697758952361767
1111	11	0.20268069828652813
1111	12	0.06348018332177219
1111	13	0.06788240678096003
1111	14	0.07191952723033523
1111	15	0.08219936963472207
1111	16	-0.032728612312972416
1111	17	0.28524206970440247
1111	18	0.41753194720770637
1111	19	0.24422974019833532
1111	20	-0.363873889055327
1111	21	-0.4157171348150399
1111	22	-0.17277169392844627
1111	23	0.05771467809881159
1111	24	0.16092434178841586
1111	25	-0.02237376559698845
1112	1	0.23828665072754163
1112	2	0.19522819352089726
1112	3	0.2768536360317846
1112	4	0.6893916821071571
1112	5	0.5494287826301445
1112	6	0.588681474800687
1112	7	0.48072735311749426
1112	8	0.535599430759973
1112	9	-1.186069554233626
1112	10	0.214677211476058
1112	11	0.19236258838797227
1112	12	0.2989977560002117
1112	13	0.26316498556919754
1112	14	0.6467046728251091
1112	15	0.39440261441280455
1112	16	0.3669653531835664
1112	17	0.41790917001902983
1112	18	0.5386888255653091
1112	19	0.307825656697581
1112	20	0.2727834296731544
1112	21	0.344773254817774
1112	22	0.40622006303219393
1112	23	0.09460718820678693
1112	24	0.27171442085365527
1112	25	0.25063118089388325
1113	1	0.30277861063756006
1113	2	0.2658166867290035
1113	3	0.3640445936382548
1113	4	0.5643372580184547
1113	5	0.6079485991205758
1113	6	0.6316615258737954
1113	7	0.5781759234790655
1113	8	0.5042374371043223
1113	9	0.30659557313314423
1113	10	0.6191517268675852
1113	11	0.4746161872652479
1113	12	0.2821803749719862
1113	13	0.3464979550249012
1113	14	0.06472685867657901
1113	15	0.3273335793694585
1113	16	0.4235707607142061
1113	17	0.7325322721758809
1113	18	0.5323264740341358
1113	19	0.42080359382924826
1113	20	0.5273775497648359
1113	21	0.4386483930837741
1113	22	0.5060975052962959
1113	23	0.10190142283664017
1113	24	0.2921260987955776
1113	25	0.33799095896244324
1114	1	0.34694587570100666
1114	2	0.41827978122163856
1114	3	0.39578423832327303
1114	4	0.6294403640866975
1114	5	0.5730531855458807
1114	6	0.6500471143621738
1114	7	0.4241616916420625
1114	8	0.4555630796891066
1114	9	0.16784058499339238
1114	10	0.3953686901944451
1114	11	0.4308048599848888
1114	12	0.26838479713485697
1114	13	0.3362923345736277
1114	14	0.8439209069914071
1114	15	0.4802698733131763
1114	16	0.2962086531140713
1114	17	0.763269895887511
1114	18	0.6220041707870863
1114	19	0.4426543160879106
1114	20	0.4800199779604313
1114	21	0.4208792806445416
1114	22	0.21235404106126055
1114	23	0.7232708986337357
1114	24	0.5001271907362295
1114	25	0.328273204250884
1115	1	0.5080330108735325
1115	2	0.48807838353036104
1115	3	0.5851342783636007
1115	4	0.8202307382895526
1115	5	0.729160388548344
1115	6	0.7631318804699632
1115	7	0.6658657392883427
1115	8	0.6422655653941476
1115	9	0.863609196433579
1115	10	0.5112480590672135
1115	11	0.5704820561714072
1115	12	0.5656677019658716
1115	13	0.5425806645661311
1115	14	0.6020126228440006
1115	15	0.6022906024214052
1115	16	0.5943753525590836
1115	17	0.8836959883228488
1115	18	1.0390198075191037
1115	19	0.8340926196863165
1115	20	0.7489402950542257
1115	21	0.6581714473284208
1115	22	0.026164905936376215
1115	23	0.47736491029295536
1115	24	0.6096996165941704
1115	25	0.6375348754737544
1116	1	0.7318271004521897
1116	2	0.6040659463909819
1116	3	0.8296339931290433
1116	4	1.1131909155748119
1116	5	0.9900688405091032
1116	6	1.0500577721237292
1116	7	0.9436490718525548
1116	8	0.8510458434295103
1116	9	1.1733127740563063
1116	10	0.9041712595857234
1116	11	0.6643812048331554
1116	12	0.6854436487731377
1116	13	0.6424399853856428
1116	14	1.1658631445054795
1116	15	0.8544790432315068
1116	16	0.8495135613688447
1116	17	1.2195388189297418
1116	18	1.2899894097990625
1116	19	0.8690163538406495
1116	20	0.8324277472503283
1116	21	0.9396788937450324
1116	22	0.9582916055893023
1116	23	1.2113766982632228
1116	24	0.9418182510264899
1116	25	0.9434331225430981
1201	1	-0.14992923809158754
1201	2	0.005001868699469725
1201	3	-0.15509186171104616
1201	4	-0.04151821607162276
1201	5	-0.31051341526289633
1201	6	-0.5991496824140796
1201	7	-0.5625431941538679
1201	8	-0.450416884109508
1201	9	0.0625240335494226
1201	10	0.17960062612315397
1201	11	0.014359073877557194
1201	12	0.03234503219504603
1201	13	-0.038408669255687755
1201	14	0.41574281997773
1201	15	0.04808171011200102
1201	16	0.010605754131475464
1201	17	0.0882875423631333
1201	18	0.22860072973723788
1201	19	0.05974598754597338
1201	20	0.08135392610001446
1201	21	-0.05474437455558245
1201	22	-0.028716469837661407
1201	23	-0.14629719690628917
1201	24	-0.21356231246632262
1201	25	-0.08476532735952702
1202	1	-0.39729257170921883
1202	2	-0.39979561919690454
1202	3	-0.5000596973864582
1202	4	-0.24346478485269074
1202	5	-0.547225328856392
1202	6	-0.7208796772411645
1202	7	-0.6333872199724695
1202	8	-0.6420053280232594
1202	9	0.11773281109812928
1202	10	0.08991364457865814
1202	11	-0.18380457994004473
1202	12	-0.1640556918167846
1202	13	-0.15531080751309645
1202	14	-0.1701770518202892
1202	15	-0.30673800421114805
1202	16	-0.2598717344358903
1202	17	0.15207323712768073
1202	18	0.25426743267638585
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2211	12	-0.21259931557803924
2211	13	-0.04282345576910984
2211	14	0.2056631870915453
2211	15	-0.06347545782564623
2211	16	-1.5459762283853706E-4
2211	17	0.21757630482180446
2211	18	0.34551164448112104
2211	19	0.01612162485694313
2211	20	0.14718812991593921
2211	21	0.026975092460212124
2211	22	0.041875140014710155
2211	23	-2.057584525166874
2211	24	-0.6392157494508481
2211	25	-0.5151651785726798
2212	1	0.031173704782450073
2212	2	-1.5078060097128088
2212	3	-0.34481314293530474
2212	4	0.2842654914022731
2212	5	0.23455016678999385
2212	6	0.4016513852813901
2212	7	0.2778925102883463
2212	8	0.24191201353809788
2212	9	0.7380146749073759
2212	10	0.5977784109271269
2212	11	0.3947773146792599
2212	12	0.2739170962749711
2212	13	0.20520478179844304
2212	14	0.4861370487028438
2212	15	0.3731091290060178
2212	16	0.36925885583726625
2212	17	0.6478322870200373
2212	18	0.8290258888801105
2212	19	0.3658859316585463
2212	20	0.4672694202061223
2212	21	0.3537232510664623
2212	22	0.4252797583318184
2212	23	0.5797741268055603
2212	24	0.3261926163904576
2212	25	0.35040079994087137
2213	1	0.19632262826254276
2213	2	0.2154232770264386
2213	3	0.24112238536345032
2213	4	0.42705374543233177
2213	5	0.3743351254876046
2213	6	0.49047264723198936
2213	7	0.33232910526943016
2213	8	0.34842173729261106
2213	9	0.6427192796168484
2213	10	0.6654178393378558
2213	11	0.35486505973033644
2213	12	0.34131689457878167
2213	13	0.17801056320634245
2213	14	-1.24595481783156
2213	15	-0.3072277850011531
2213	16	0.052317973009564867
2213	17	0.47779148423214224
2213	18	0.3089052008056328
2213	19	0.17111429747649964
2213	20	0.27460256502872227
2213	21	0.3400260920159397
2213	22	0.3187359120741675
2213	23	0.5300173365561989
2213	24	0.2532121792721753
2213	25	0.10730706581104954
2214	1	0.31264073290832073
2214	2	0.2983545176401954
2214	3	0.23998715481396005
2214	4	0.502331443587785
2214	5	0.38613974701208775
2214	6	0.4129315314905355
2214	7	0.288782433114946
2214	8	0.14637611737772005
2214	9	0.3077319456906338
2214	10	0.1384176041457721
2214	11	0.09272939309551731
2214	12	-0.01104433596580634
2214	13	0.009188581566029086
2214	14	0.27694425536462575
2214	15	0.02580941454708352
2214	16	0.11036938207965363
2214	17	0.073451577769994
2214	18	0.38789421782521316
2214	19	0.09105558520217016
2214	20	0.22601610621305923
2214	21	0.31348246020915127
2214	22	0.28842430705967814
2214	23	0.2638221565240073
2214	24	0.20699570945638612
2214	25	0.17228102318738792
2215	1	0.3627278804550329
2215	2	0.26582361293072765
2215	3	0.3304258494059944
2215	4	0.6877399865308291
2215	5	0.4662231513438684
2215	6	0.582494486532795
2215	7	0.48540379703504044
2215	8	0.4335352726260666
2215	9	0.6183751922975063
2215	10	0.7098033743115835
2215	11	0.37928458351619554
2215	12	0.3308228439028369
2215	13	0.20732823099874054
2215	14	0.39451843407378107
2215	15	0.3633762707788364
2215	16	0.4190886926951478
2215	17	0.5356077671490667
2215	18	0.6761819857803459
2215	19	0.371977547038874
2215	20	-0.5588831203840527
2215	21	-0.0248874114468407
2215	22	-0.694460440469129
2215	23	0.2898183578377669
2215	24	0.16531341126307808
2215	25	0.33042752433854616
2216	1	0.2516750427511312
2216	2	-0.15665456707792202
2216	3	0.27366640470880554
2216	4	0.5425365518953242
2216	5	0.4300205987548509
2216	6	0.6291212678733231
2216	7	0.49537808191052335
2216	8	0.44469299915696325
2216	9	0.7479106290473823
2216	10	0.3954698165132271
2216	11	0.3670039257835427
2216	12	-0.07065204854013984
2216	13	0.19499866680217082
2216	14	0.23421015320332117
2216	15	0.20162165672450527
2216	16	0.2939196841108327
2216	17	0.37059589223425604
2216	18	0.7399834653849098
2216	19	0.46623623783487034
2216	20	-0.7655481281099625
2216	21	-0.9828846271725382
2216	22	-1.0262152344789754
2216	23	-1.4220500364767403
2216	24	-1.1116583961440583
2216	25	-0.7953388748291559
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11920.0
3	1518.0
4	1415.0
5	1088.0
6	1182.0
7	1572.0
8	725.0
9	1338.0
10	3154.0
11	6785.0
12	12310.0
13	19121.0
14	26170.0
15	32245.0
16	36976.0
17	40269.0
18	41752.0
19	42239.0
20	43128.0
21	43196.0
22	44935.0
23	46582.0
24	49414.0
25	52805.0
26	57977.0
27	66006.0
28	75859.0
29	91393.0
30	111504.0
31	140772.0
32	182373.0
33	240444.0
34	326776.0
35	456775.0
36	666402.0
37	1064036.0
38	1913052.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.59272120835939	19.672231220031787	13.427340310087818	24.307707261521003
2	16.15137317202426	20.42589070902245	39.177607310875736	24.245128808077556
3	18.63388583987712	24.99829819986615	29.781923580075258	26.585892380181477
4	13.76722413573418	16.994328903378992	35.88871450424085	33.349732456645974
5	14.048529330956915	36.966835908252875	34.830521873359736	14.154112887430466
6	32.01315655795141	36.47462272700698	17.00649555346149	14.505725161580116
7	27.30752615269318	31.77050127174673	22.775706874171885	18.146265701388206
8	25.301412117286155	35.95747470369399	20.165020016622663	18.576093162397196
9	25.704405595277528	17.013200980915816	20.620833306654813	36.661560117151836
10	16.039989152216016	28.927513846683865	32.818574858052635	22.213922143047483
11	33.34486390501055	24.418281536141606	23.254086756829675	18.982767802018174
12	22.351050339959084	26.04273270693832	30.115114658054438	21.491102295048158
13	27.262988336996642	23.01216798861745	26.161066041055403	23.56377763333051
14	22.12062619940919	22.34175884597969	26.636351262479245	28.901263692131874
15	23.37773826079278	29.891221184938814	23.589151509064227	23.14188904520418
16	23.352498320663354	29.54141813494927	24.752226258020222	22.353857286367155
17	22.348617687459566	28.8721493822057	26.713665287424043	22.065567642910697
18	22.406185887273804	28.73006556450197	27.393808519577874	21.469940028646352
19	22.84716096872217	28.010685699410367	27.547891461465685	21.594261870401777
20	23.389270985883233	27.536870932996926	27.331405958804268	21.742452122315576
21	23.6318145576279	27.551413106407757	26.7829882652824	22.033784070681943
22	23.553828550178913	27.053523880291852	27.224081754602132	22.168565814927106
23	23.667868243990192	27.314344179411147	26.866695304250186	22.15109227234847
24	23.28150867828263	27.22854551592304	26.9621058319919	22.527839973802426
25	23.546114175707878	27.183075318222944	26.83120207748387	22.439608428585313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16666.0
1	16666.0
2	13920.0
3	11174.0
4	11174.0
5	11174.0
6	10812.0
7	10450.0
8	10450.0
9	10450.0
10	13646.5
11	16843.0
12	16843.0
13	16843.0
14	25767.0
15	34691.0
16	34691.0
17	34691.0
18	56845.0
19	78999.0
20	78999.0
21	78999.0
22	117395.0
23	155791.0
24	155791.0
25	155791.0
26	211087.5
27	266384.0
28	266384.0
29	266384.0
30	332882.0
31	399380.0
32	399380.0
33	399380.0
34	464340.0
35	529300.0
36	529300.0
37	529300.0
38	588436.5
39	647573.0
40	647573.0
41	647573.0
42	680607.0
43	713641.0
44	713641.0
45	713641.0
46	714153.5
47	714666.0
48	714666.0
49	714666.0
50	699324.0
51	683982.0
52	683982.0
53	683982.0
54	637685.0
55	591388.0
56	591388.0
57	591388.0
58	517750.5
59	444113.0
60	444113.0
61	444113.0
62	373247.0
63	302381.0
64	302381.0
65	302381.0
66	241342.5
67	180304.0
68	180304.0
69	180304.0
70	136653.0
71	93002.0
72	93002.0
73	93002.0
74	66894.0
75	40786.0
76	40786.0
77	40786.0
78	28494.5
79	16203.0
80	16203.0
81	16203.0
82	10917.5
83	5632.0
84	5632.0
85	5632.0
86	3554.0
87	1476.0
88	1476.0
89	1476.0
90	898.5
91	321.0
92	321.0
93	321.0
94	181.5
95	42.0
96	42.0
97	42.0
98	31.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14607382311415487
2	0.28339900134470536
3	0.3411467743863858
4	0.36882003113913064
5	0.37724962755289154
6	0.33444675651967154
7	0.3369151841547768
8	0.35498340276275825
9	0.34165053512824406
10	0.37276615695035337
11	0.29167746953590873
12	0.3372174405998917
13	0.30899004703110283
14	0.3182424526565655
15	0.35820747151065085
16	0.3079657335226578
17	0.35725032610112023
18	0.32532868709203777
19	0.338745514850195
20	0.3012992997054007
21	0.34154978297987243
22	0.29611056406426106
23	0.2791674111130963
24	0.315354224403245
25	0.30915796727838896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5955208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.196606624841564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.62895688545724	43.257196964246006
2	13.449343373311853	15.385136045714754
3	4.628925235004347	7.942764472870378
4	2.0570946553754466	4.706353351742938
5	1.1950419962501075	3.417617347984139
6	0.71134473170252	2.44119028763055
7	0.4661927887184171	1.8665251883365621
8	0.33203484398253585	1.5193013085607796
9	0.2408284952318423	1.2397115435245396
>10	1.1994757534261096	12.00483420353146
>50	0.0651204244938737	2.552222011333299
>100	0.024521002086977464	2.3471973776348904
>500	5.599141623912835E-4	0.21315203860922546
>1k	4.4202694446649653E-4	0.6387018859673558
>5k	1.1787385182255024E-4	0.46809597231304073
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	8123	0.13640161687047705	No Hit
GGTATCAACGCAGAGTACATGGGGG	7114	0.1194584639193123	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6846	0.11495820129204554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.6792024728607296E-5	0.0	0.0	0.0
6	1.6792024728607296E-5	1.6792024728607296E-5	0.0	0.0	0.0
7	1.6792024728607296E-5	1.6792024728607296E-5	0.0	0.0	0.0
8	1.6792024728607296E-5	1.6792024728607296E-5	0.0	0.0	0.0
9	1.6792024728607296E-5	1.6792024728607296E-5	0.0	0.0	0.0
10	1.6792024728607296E-5	1.6792024728607296E-5	0.0	0.0	0.0
11	1.6792024728607296E-5	1.6792024728607296E-5	0.0	0.0	0.0
12	1.6792024728607296E-5	3.358404945721459E-5	0.0	0.0	0.0
13	1.6792024728607296E-5	3.358404945721459E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	1815	0.0	11.995445	1
TCGACCG	115	7.107883E-8	11.562795	9
TGGTATC	1745	0.0	11.330675	2
GGTATCA	3970	0.0	11.039971	1
GTACCGT	185	6.7338988E-9	9.241463	6
TAGACAG	755	0.0	9.064965	5
GTATTAG	630	0.0	8.903668	1
CGCCTAA	165	1.0541753E-6	8.634846	14
GTCTTAG	520	0.0	8.41031	1
TGCGTTA	125	2.750899E-4	8.358532	13
AGGACCG	220	1.4517354E-8	8.209402	5
CTAGACA	465	0.0	8.1771555	4
GTCGTAA	140	1.0308322E-4	8.14912	1
TCTAGAC	490	0.0	8.147814	3
GTATCAA	7150	0.0	8.111129	1
ACAGTCG	190	9.2035407E-7	7.9984593	8
CCGCCTA	180	3.6593556E-6	7.915276	13
GTGTAGA	1015	0.0	7.8681164	1
AGGACTG	1200	0.0	7.842139	5
TAATACG	170	1.4415757E-5	7.828424	4
>>END_MODULE