FastQCFastQC Report
Wed 25 May 2016
SRR1295171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5955208
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT172490.28964563454374725No Hit
GGTATCAACGCAGAGTACTTTTTTT135340.22726326267697114No Hit
TATCAACGCAGAGTACTTTTTTTTT96210.1615560699139308No Hit
GTGGTATCAACGCAGAGTACATGGG91750.15406682688497195No Hit
GGTATCAACGCAGAGTACATGGGGG78570.13193493829266753No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61590.10342208030349234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20700.012.3930141
GGTATCA53550.011.9764411
TGGTATC20450.011.798862
TCGACCG1658.0035534E-1110.9402599
GCGCGAC803.778034E-410.68628116
CGTAGAC1951.6370905E-109.7434613
TAGACAG8100.09.2655435
AGCGTCA5600.09.1605933
CGAATTA1151.11307556E-49.08592115
TAGACCG950.00182483859.0001085
GTATTAA9400.08.9959211
GTCTACG1701.684275E-78.94240951
CGCGACT850.00744687868.94015717
GTACTAG3950.08.8999621
TAACCCG1107.1447884E-48.63697410
CTAGACT5300.08.603664
CCTACAC8200.08.4572053
GAACCGT1356.803856E-58.4445466
GTATTAG6550.08.4133741
TCTAGAC4750.08.3998893
Produced by FastQC (version 0.11.5)