FastQCFastQC Report
Wed 25 May 2016
SRR1295170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81461
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA9801.2030296706399382No Hit
GTATCAACGCAGAGTACTTTTTTTT1510.18536477578227617No Hit
GTACATAAGCAGTGGTATCAACGCA1190.1460821742919925No Hit
GGTATCAACGCAGAGTACTTTTTTT1090.13380636132627882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAAA400.00520321214.2578552
ATAAAAC550.003013119312.0975753
GTATCAA1304.1829022E-58.7740651
CATGGGG1050.00439710038.14734654
TCAACGC1450.00129609087.210874
AACGCAG1500.001868076.9662156
TATCAAC1550.00262237156.7456522
ATCAACG1550.00262237156.7456523
GTACATG1750.0012933516.5178771
CAACGCA1650.00504710256.33682445
Produced by FastQC (version 0.11.5)