##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295170_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81461
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.517008138863996	30.0	24.0	31.0	15.0	34.0
2	26.536514405666516	31.0	24.0	31.0	10.0	34.0
3	26.410932839027264	31.0	23.0	31.0	10.0	34.0
4	28.982126416321922	35.0	23.0	37.0	10.0	37.0
5	29.454462871803685	35.0	26.0	37.0	10.0	37.0
6	29.66661347147715	35.0	26.0	37.0	11.0	37.0
7	29.53867494874848	35.0	26.0	37.0	10.0	37.0
8	29.560022587495858	35.0	26.0	37.0	10.0	37.0
9	30.215121346411166	35.0	25.0	39.0	10.0	39.0
10	30.160764046598985	35.0	25.0	39.0	10.0	39.0
11	30.63441401406808	35.0	27.0	39.0	10.0	39.0
12	30.558819557825217	35.0	26.0	39.0	10.0	39.0
13	30.431531653183733	35.0	25.0	39.0	10.0	39.0
14	30.83128122659923	36.0	24.0	40.0	10.0	41.0
15	31.15207277101926	37.0	25.0	40.0	10.0	41.0
16	31.173592271148156	37.0	25.0	40.0	10.0	41.0
17	30.860313524263145	36.0	25.0	40.0	10.0	41.0
18	30.731810314138055	36.0	24.0	40.0	10.0	41.0
19	31.01067995728017	36.0	25.0	40.0	10.0	41.0
20	30.9527749475209	36.0	25.0	40.0	10.0	41.0
21	30.934078884374117	36.0	25.0	40.0	10.0	41.0
22	30.874295675231092	36.0	24.0	40.0	10.0	41.0
23	30.665741888756582	36.0	24.0	40.0	10.0	41.0
24	30.80955303764992	36.0	24.0	40.0	10.0	41.0
25	29.45221639803096	34.0	18.0	40.0	9.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.04834154349690323
1101	2	0.42724326861683437
1101	3	-0.2651405427375906
1101	4	-0.7229488528219221
1101	5	-0.7351806822389513
1101	6	-1.0468727404413372
1101	7	-0.3054132001210945
1101	8	-0.9344262017282468
1101	9	-0.8039133654612449
1101	10	-0.48251519704876955
1101	11	-0.3136086491871879
1101	12	-0.3595948224636665
1101	13	0.2554705554960961
1101	14	-0.3848352184854278
1101	15	0.25882369438647146
1101	16	-0.9380202981585946
1101	17	0.44015558523706844
1101	18	-0.5225781957461528
1101	19	-0.40321370550909563
1101	20	0.09804376591901587
1101	21	-0.5962642040267845
1101	22	-0.23511003667915276
1101	23	-0.0786544850842752
1101	24	0.18253654142523246
1101	25	0.3719186958504288
1102	1	-0.2588487898737135
1102	2	0.30296790629799375
1102	3	-0.21187967317237266
1102	4	-0.14432566441612593
1102	5	-0.7109053199201085
1102	6	-0.6244089723253943
1102	7	-0.5901958088167483
1102	8	-0.4058030133224513
1102	9	-0.18108727850472306
1102	10	-0.5796166463241335
1102	11	0.10813048124759561
1102	12	-1.059232503623086
1102	13	-0.7162685749386881
1102	14	0.022773477166747824
1102	15	-0.01907485633816819
1102	16	-0.0054116025064203654
1102	17	0.23182225190373273
1102	18	0.7027841230944283
1102	19	-0.35285138666851523
1102	20	0.27739159200597285
1102	21	0.2182285495964038
1102	22	-0.19924047146175994
1102	23	-0.04713274595383865
1102	24	0.4405075559179856
1102	25	-0.07228420270029545
1103	1	-0.8823336383585634
1103	2	-1.656191873316331
1103	3	-1.3389457888748524
1103	4	-1.5665708434794858
1103	5	-1.6699273584876018
1103	6	-1.5193125535926129
1103	7	-2.211270188982038
1103	8	-2.0127589361874634
1103	9	-0.6461699231328204
1103	10	0.43828968976402294
1103	11	-0.07451414685158042
1103	12	-0.438502476074877
1103	13	-0.436516508822983
1103	14	-0.47495379556052697
1103	15	-0.7181795395337573
1103	16	-0.7785520983741883
1103	17	-0.953232844515
1103	18	-0.5766924609276103
1103	19	-0.6507164004426222
1103	20	-1.032319151795683
1103	21	-0.6203389435165434
1103	22	-0.9758933640237437
1103	23	-3.0213062996728475
1103	24	-1.2492996066164466
1103	25	-0.3831657454000208
1104	1	-2.480176473489532
1104	2	-1.5943484778827965
1104	3	-1.739422046053729
1104	4	-2.8037889412522823
1104	5	-2.4220635120105065
1104	6	-1.3738440005739818
1104	7	-2.5876534359353904
1104	8	-1.745774764734879
1104	9	-3.6340533801996386
1104	10	-2.0061703186405175
1104	11	-3.0088186712947795
1104	12	-2.4593737465609387
1104	13	-3.112031286803095
1104	14	-1.2107010991044405
1104	15	-2.4836228789370374
1104	16	-2.1316827889470993
1104	17	-1.8801833978137807
1104	18	-0.8087977978095253
1104	19	-1.809772290623318
1104	20	-1.756930441892333
1104	21	-2.578946591646531
1104	22	-1.7335625053600658
1104	23	-2.3119632544284165
1104	24	-2.2295489412571534
1104	25	-3.256747479536454
1105	1	1.3251255691006456
1105	2	0.911301239631328
1105	3	0.6866246003318999
1105	4	0.9264008313103709
1105	5	0.6491374151226239
1105	6	1.1008047028882793
1105	7	0.439291370670432
1105	8	0.4687696362501974
1105	9	-0.5330103554277983
1105	10	0.9887594220519347
1105	11	1.3158227889399008
1105	12	1.0234598040692227
1105	13	1.1850134763433644
1105	14	1.12255980195307
1105	15	1.1328482205849113
1105	16	1.0514260043311872
1105	17	1.2675060125874928
1105	18	1.433126003436307
1105	19	1.5200118611947318
1105	20	0.9220497116640907
1105	21	0.2985704299382874
1105	22	1.022340725119438
1105	23	1.7274399036188086
1105	24	1.3242682396786734
1105	25	2.2768610964450033
1106	1	-0.9505154565403799
1106	2	-0.29846887531120103
1106	3	-0.40584519041375344
1106	4	-1.3911647448758941
1106	5	-0.666365090035054
1106	6	-0.7870526504863165
1106	7	-0.32640270536846927
1106	8	-0.2095386455063597
1106	9	-2.490570037125412
1106	10	-1.1198465313816044
1106	11	-0.9142833118558542
1106	12	-0.5198646875311326
1106	13	0.3061452181647617
1106	14	0.15122175302881402
1106	15	-0.4210863505910396
1106	16	-0.5726529818167663
1106	17	0.21084524040947983
1106	18	-0.39836530219292854
1106	19	-0.36865598436966707
1106	20	0.3368743506266618
1106	21	0.2170791243090484
1106	22	-0.5190680576686582
1106	23	0.10372932301167737
1106	24	0.5428064064927014
1106	25	0.5869111995985534
1107	1	0.6277288182687819
1107	2	0.8705887714379443
1107	3	1.115410403163505
1107	4	-0.7245419494034024
1107	5	-0.050281910250898676
1107	6	0.6037080760206592
1107	7	0.7636209850763827
1107	8	0.8090188696292024
1107	9	1.2843707367624546
1107	10	2.04506528751811
1107	11	1.5882831530033208
1107	12	1.1712382342903993
1107	13	1.1642657762063493
1107	14	1.293956683273617
1107	15	1.0075790876821884
1107	16	0.9713059547454854
1107	17	1.748552531801952
1107	18	0.534972418259823
1107	19	1.1245659161304644
1107	20	0.6420862484945218
1107	21	0.48438631041065605
1107	22	1.8028587651794616
1107	23	1.8564932082446362
1107	24	1.309718751846738
1107	25	1.3110491306330374
1108	1	1.26823454345962
1108	2	0.5727595729646602
1108	3	1.1865578268276273
1108	4	1.639007668917209
1108	5	1.5911780134132236
1108	6	0.9516326943412707
1108	7	1.1426166911832532
1108	8	0.4494053200108823
1108	9	1.8954752214952784
1108	10	1.7433000203425344
1108	11	0.7893804812475942
1108	12	1.0491008297102482
1108	13	1.1024814250613133
1108	14	1.2352734771667464
1108	15	0.9048834769951668
1108	16	0.9445883974935789
1108	17	1.222968085237067
1108	18	2.378304956427762
1108	19	2.519544446664817
1108	20	1.2945790920059714
1108	21	1.8843743829297388
1108	22	1.9820095285382386
1108	23	2.1169297540461613
1108	24	2.0597783892513206
1108	25	1.6079241306330374
1109	1	0.5120829686564718
1109	2	0.205264494224501
1109	3	0.27138804336306066
1109	4	0.4569210547439795
1109	5	0.4127823441218865
1109	6	0.7536750171759152
1109	7	0.06295379748246432
1109	8	-0.012824010697780608
1109	9	0.6777709892118153
1109	10	0.3429555321535567
1109	11	0.5041934733735793
1109	12	0.25382523915906674
1109	13	0.6612904801794244
1109	14	0.6402931622061168
1109	15	0.11366792581406315
1109	16	-0.7365139647111469
1109	17	0.2023603096465152
1109	18	0.05147178713642475
1109	19	0.03855281280655021
1109	20	0.9756199384626676
1109	21	0.16888471757540913
1109	22	-0.024634172249161423
1109	23	0.8495207973532466
1109	24	0.6195101805899021
1109	25	-0.09052567251656995
1110	1	0.18999549236473
1110	2	0.10594844157779804
1110	3	-0.02483587755193639
1110	4	0.10707336234786524
1110	5	0.21823093312125152
1110	6	-0.15591748814048145
1110	7	-0.39713011538609067
1110	8	-0.38031639531758543
1110	9	0.7044966631011178
1110	10	0.6089478305615117
1110	11	0.47528376591912647
1110	12	0.16588915087813305
1110	13	0.00234456374744596
1110	14	-0.2752192235631803
1110	15	-1.0924933478223515
1110	16	0.006632193114015195
1110	17	-0.3058298162227864
1110	18	-0.06278765306128875
1110	19	-0.3507224328972285
1110	20	-0.29249891894293256
1110	21	-0.1462483360483624
1110	22	0.015540550436050182
1110	23	0.45013002776878963
1110	24	-0.06265546111364273
1110	25	-0.754644300023898
1111	1	-0.14732396717867857
1111	2	-0.7128122355459787
1111	3	0.09397139065741555
1111	4	0.2037239809739475
1111	5	-0.18519610006195464
1111	6	-0.3403664191339075
1111	7	-0.045160347823838265
1111	8	-0.37229237502457835
1111	9	-0.3426121011997587
1111	10	-0.5335173555439887
1111	11	-0.9067631357736836
1111	12	-0.8870693830557101
1111	13	-0.667687014654998
1111	14	-0.9610917710602038
1111	15	-0.7809542889622811
1111	16	-1.1015108932865623
1111	17	0.45008466325124985
1111	18	-0.7734570116573458
1111	19	-1.8200499682288012
1111	20	-1.1976084079940286
1111	21	-1.028579961041892
1111	22	-0.8196305423837487
1111	23	-0.8015653700673155
1111	24	-1.2531094653586088
1111	25	-0.4620005147570332
1112	1	-0.9402414839376405
1112	2	-0.22111885169287504
1112	3	0.24188745011529988
1112	4	-0.1811978105348473
1112	5	-0.2662706167237623
1112	6	-0.2707560042888666
1112	7	-0.2333464937482539
1112	8	-0.03925050190692758
1112	9	-1.0314297442581477
1112	10	-0.5636349111643142
1112	11	-0.7041982858756946
1112	12	-0.26596766344043843
1112	13	-0.5169535064455353
1112	14	-0.024144331052433188
1112	15	-0.1586867284842839
1112	16	-0.4458225614105302
1112	17	-0.48861153805060553
1112	18	0.5368023879346104
1112	19	-0.2027372314173732
1112	20	-0.5537727915556729
1112	21	-0.2004929115908105
1112	22	-0.6742404714617614
1112	23	-0.29199575965246893
1112	24	-0.9371180148582674
1112	25	-0.01771799265463514
1113	1	1.653651210126288
1113	2	1.3696345729646602
1113	3	1.2811758823831845
1113	4	2.2501187800283198
1113	5	2.594650235635445
1113	6	2.2242021387857136
1113	7	1.9959153022943639
1113	8	1.8955858755664394
1113	9	1.3911349437174998
1113	10	2.0870500203425344
1113	11	1.890074925692037
1113	12	1.2991008297102482
1113	13	1.0226203139502026
1113	14	1.7352734771667464
1113	15	1.000369588106281
1113	16	1.507088397493579
1113	17	2.0762666963481813
1113	18	2.0805619008722047
1113	19	2.1888152799981526
1113	20	1.9204471475615286
1113	21	1.7515618829297388
1113	22	2.2146484174271244
1113	23	1.1820339207128256
1113	24	1.4668964448068778
1113	25	2.581882463966373
1114	1	1.1736104175854933
1114	2	0.6502289785590669
1114	3	1.098435012142314
1114	4	1.0926789975885391
1114	5	1.331344097329307
1114	6	0.8824674845510607
1114	7	0.4765874079664698
1114	8	1.208474375954939
1114	9	1.2851714627540183
1114	10	0.8457912790837945
1114	11	1.0011374742545875
1114	12	1.6015483821578016
1114	13	1.2526125439424334
1114	14	1.9795916589849263
1114	15	1.2144333021699936
1114	16	1.6333995863047654
1114	17	1.3786171236986071
1114	18	1.2567468270571354
1114	19	1.38170572288859
1114	20	1.550643340257718
1114	21	1.0172961486640055
1114	22	0.7628224656011753
1114	23	1.0988462750251813
1114	24	1.0157989312093605
1114	25	1.4229372425211508
1115	1	1.3365215804966581
1115	2	1.2964864248165107
1115	3	1.2501573638646661
1115	4	1.3640076689172105
1115	5	2.099279865265075
1115	6	2.210776212859791
1115	7	1.1338782652573265
1115	8	1.2710488385293992
1115	9	2.3365053140878693
1115	10	1.9796426129351268
1115	11	1.726648999766116
1115	12	2.2046563852658068
1115	13	1.4448425361724233
1115	14	1.7533290327223021
1115	15	1.3225918103284968
1115	16	2.6001439530491375
1115	17	1.840155585237067
1115	18	2.54537671568702
1115	19	1.6128893540722231
1115	20	2.032021221635599
1115	21	2.1145248458926993
1115	22	1.492426195204903
1115	23	1.8852746614535718
1115	24	1.8775445929550258
1115	25	2.8221602417441503
1116	1	1.14822403925794
1116	2	0.8832900351495354
1116	3	1.4551371335503163
1116	4	1.8396379210180491
1116	5	2.1045708705560813
1116	6	1.9409971901395906
1116	7	2.2934176365614043
1116	8	1.362614353624327
1116	9	1.6111396122515806
1116	10	1.3433525413509386
1116	11	2.2770380442728033
1116	12	2.040697468365707
1116	13	2.209361677162157
1116	14	1.9238238973348167
1116	15	1.6245525946422248
1116	16	2.1168573050566017
1116	17	1.7141051650689967
1116	18	1.1970418261756635
1116	19	1.3869945517068345
1116	20	1.4494504155353845
1116	21	1.3061837316692326
1116	22	1.7165158310592439
1116	23	1.5232454053066675
1116	24	1.2091350068983786
1116	25	1.7900407272716912
1201	1	-0.6759252926059531
1201	2	0.515126376243348
1201	3	-0.5131091813690922
1201	4	-0.5638611835418068
1201	5	-1.416506412816286
1201	6	-0.07167673188823898
1201	7	-1.0024908907839603
1201	8	-0.3638631226120701
1201	9	-0.6200217047342313
1201	10	-0.23262211080500705
1201	11	-0.8279350925228961
1201	12	-0.39352212110942375
1201	13	-0.202334148709177
1201	14	-0.21042324414472802
1201	15	-0.16811857218516124
1201	16	-0.3668870123424881
1201	17	-0.3155821196809647
1201	18	-0.007325166523056481
1201	19	-0.2059934631712501
1201	20	-0.5500674243874712
1201	21	-0.42876598592271975
1201	22	0.047071003948076395
1201	23	-0.5122966803800679
1201	24	-0.3048476353388416
1201	25	-1.0987869349407333
1202	1	0.11766636164143662
1202	2	-0.22643980720063084
1202	3	-0.24803669796576244
1202	4	-0.4839262153803112
1202	5	-0.27323314361157003
1202	6	-0.006915859377738087
1202	7	0.8961678275468898
1202	8	0.17732370844063539
1202	9	0.9819292504208974
1202	10	0.3391161360450141
1202	11	0.8345767622393296
1202	12	-0.06660164962859483
1202	13	0.31554960687949674
1202	14	0.0787652127039351
1202	15	-0.21404730812880146
1202	16	0.5107040999729158
1202	17	0.28643657697260494
1202	18	0.7621505142789999
1202	19	-0.08873292936824129
1202	20	0.025532087873738618
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2211	14	-1.3460621392716092
2211	15	-1.329919605196615
2211	16	-0.7540417394927239
2211	17	-0.43381701750265833
2211	18	-0.8947044613804565
2211	19	-0.35342216292422535
2211	20	-1.6770604627885497
2211	21	-2.1319997609058774
2211	22	-1.6536925262562825
2211	23	-1.9426806911593175
2211	24	-1.259035823077447
2211	25	-0.16840292416148372
2212	1	-1.3031644631628971
2212	2	-1.5235773475651406
2212	3	-1.0769458983379359
2212	4	-0.22936981452649974
2212	5	-0.6532342382424048
2212	6	-0.007080054003100855
2212	7	0.18695650906404637
2212	8	-0.3176683554858073
2212	9	0.053846496329715166
2212	10	0.0042685633888908114
2212	11	0.4981967064131574
2212	12	0.2543988429552826
2212	13	-0.1245467206340507
2212	14	0.9236012917362864
2212	15	0.5150862915647068
2212	16	-1.005742728334237
2212	17	-0.2658046796635958
2212	18	0.23711083391120624
2212	19	0.0690581056052153
2212	20	-0.24396602388806699
2212	21	0.4270585716714592
2212	22	0.25622310469717746
2212	23	0.11097983682761736
2212	24	0.1658938693837726
2212	25	-0.4968978892345106
2213	1	1.038046634962889
2213	2	0.7479025468208711
2213	3	0.5307673856511563
2213	4	1.6006089761067521
2213	5	0.927166575504728
2213	6	0.34715965512558355
2213	7	1.3643793545819456
2213	8	0.8780205160893146
2213	9	1.3609061855475666
2213	10	0.9530630922379579
2213	11	0.7989801544502093
2213	12	0.761519130363844
2213	13	0.820005934865236
2213	14	-0.6466709672776965
2213	15	-0.11400949686104411
2213	16	0.7909936262517512
2213	17	1.8270836898122305
2213	18	0.9841566721140396
2213	19	0.866919855161548
2213	20	0.933110546254337
2213	21	0.19600632737418167
2213	22	1.139485018734316
2213	23	1.6268868618892967
2213	24	0.9673049415389023
2213	25	1.3894805031820567
2214	1	1.0137702577453354
2214	2	1.3946345729646588
2214	3	1.505977469684769
2214	4	1.4354362403457799
2214	5	1.2974280134132243
2214	6	1.235114837198413
2214	7	1.488375619754681
2214	8	1.0239588914394524
2214	9	1.1081984357809915
2214	10	0.2727643060568212
2214	11	0.6081304812475956
2214	12	0.7562436868531037
2214	13	-0.10912571779583047
2214	14	0.9549163343096048
2214	15	1.3464013341380223
2214	16	1.9088741117792942
2214	17	1.5758698709513546
2214	18	1.0004031707134757
2214	19	1.6126248038076767
2214	20	1.3595344491488284
2214	21	0.7801333115011708
2214	22	0.9329023856810927
2214	23	1.3350101111890211
2214	24	1.1524123178227512
2214	25	0.003906273490180467
2215	1	0.6605956545707308
2215	2	0.4946345729646602
2215	3	1.086731437938738
2215	4	1.6528965578060983
2215	5	0.4557613467465558
2215	6	0.7172576943412707
2215	7	1.4681375245165853
2215	8	0.7705858755664394
2215	9	1.155023832606389
2215	10	1.1564944647869773
2215	11	0.7234082590253728
2215	12	1.5282674963769125
2215	13	1.154564758394649
2215	14	0.9574956993889678
2215	15	0.36842514366183465
2215	16	0.8126439530491361
2215	17	1.5971000296815099
2215	18	2.0944507897610976
2215	19	1.5221486133314812
2215	20	0.9898915920059714
2215	21	0.7307285495964067
2215	22	0.4854817507604601
2215	23	1.2514783651572756
2215	24	1.4182853336957635
2215	25	1.651326908410816
2216	1	0.9735586175336941
2216	2	0.48907901740910376
2216	3	0.5983055120128142
2216	4	1.9121558170653579
2216	5	2.0548354208206305
2216	6	1.2626280647116417
2216	7	1.5412856726647348
2216	8	1.6710488385294013
2216	9	1.4698386474212057
2216	10	1.2240870573795704
2216	11	1.0155378886550004
2216	12	0.8416934223028392
2216	13	-0.2144167230868348
2216	14	1.301477180870453
2216	15	1.8855547732914602
2216	16	1.0445883974935803
2216	17	0.5734889185704013
2216	18	0.9009322712425742
2216	19	0.9758523170351907
2216	20	1.5283175179318995
2216	21	-0.0632529318850743
2216	22	-0.7001663973876866
2216	23	-0.06287348669457771
2216	24	-0.21875170334127247
2216	25	-0.31117309158918616
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155.0
3	32.0
4	14.0
5	18.0
6	19.0
7	40.0
8	103.0
9	238.0
10	459.0
11	792.0
12	1203.0
13	1575.0
14	2013.0
15	2215.0
16	2424.0
17	2358.0
18	2188.0
19	2013.0
20	1791.0
21	1704.0
22	1516.0
23	1323.0
24	1277.0
25	1120.0
26	1211.0
27	1122.0
28	1206.0
29	1342.0
30	1489.0
31	1710.0
32	2254.0
33	2663.0
34	3326.0
35	4495.0
36	6667.0
37	10257.0
38	17129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91824919797927	20.923829541403936	11.771574664749192	24.386346595867593
2	18.824615384615385	22.484923076923078	34.574769230769235	24.115692307692306
3	20.58718488688563	26.82971884582702	26.79646802379281	25.786628243494537
4	17.031944906432102	19.04497911815796	33.00686205664584	30.91621391876409
5	15.824227267687533	38.560851514666204	31.384820075640913	14.230101142005347
6	34.660196566248736	36.24898391506762	15.61814912431953	13.472670394364117
7	28.66151041923247	33.714715010591654	19.64136164343071	17.98241292674516
8	27.549977213100618	34.8922857107665	19.200118245531918	18.35761883060096
9	28.837982634398667	17.92351745797155	19.086150625038485	34.152349282591295
10	18.296168535173095	30.156461746950843	29.61438955279044	21.93298016508562
11	33.961985424463265	26.148562142997832	20.9757238526689	18.91372857987
12	24.165239989654026	28.16198839772881	26.663053786749764	21.009717825867398
13	29.37055187529244	24.499470534636885	23.97493043071392	22.155047159356762
14	24.879021831480184	24.547794072377574	24.484996244443625	26.08818785169862
15	25.83482170351666	30.616493194555645	22.177742193755005	21.37094290817269
16	25.15761993892227	30.258102649985226	22.979263126785536	21.605014284306964
17	24.195018600182305	29.613707472099726	24.331748417136804	21.85952551058116
18	23.578166949924874	29.12017537377768	26.06098672381093	21.240670952486514
19	23.797970343366668	29.028523572589783	26.309177792009457	20.86432829203409
20	24.800837283753	28.317429046358434	25.88930616265468	20.992427507233884
21	25.86005148607536	28.47992905268085	24.675132718292335	20.98488674295146
22	24.94429055093875	28.74238227146814	25.028008618036317	21.28531855955679
23	25.35544148602238	28.59288255351625	24.966456171448968	21.085219789012395
24	25.84192185980077	26.899634295002034	25.07603462499846	22.182409220198736
25	24.82270376754494	28.091603053435115	25.43831568579168	21.647377493228266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1170.0
1	1170.0
2	763.0
3	356.0
4	356.0
5	356.0
6	318.5
7	281.0
8	281.0
9	281.0
10	321.5
11	362.0
12	362.0
13	362.0
14	443.5
15	525.0
16	525.0
17	525.0
18	815.5
19	1106.0
20	1106.0
21	1106.0
22	1668.5
23	2231.0
24	2231.0
25	2231.0
26	2804.5
27	3378.0
28	3378.0
29	3378.0
30	4078.0
31	4778.0
32	4778.0
33	4778.0
34	5381.0
35	5984.0
36	5984.0
37	5984.0
38	6678.0
39	7372.0
40	7372.0
41	7372.0
42	7983.5
43	8595.0
44	8595.0
45	8595.0
46	8645.5
47	8696.0
48	8696.0
49	8696.0
50	8635.0
51	8574.0
52	8574.0
53	8574.0
54	8352.5
55	8131.0
56	8131.0
57	8131.0
58	7302.0
59	6473.0
60	6473.0
61	6473.0
62	5845.5
63	5218.0
64	5218.0
65	5218.0
66	4584.0
67	3950.0
68	3950.0
69	3950.0
70	3063.0
71	2176.0
72	2176.0
73	2176.0
74	1653.0
75	1130.0
76	1130.0
77	1130.0
78	848.0
79	566.0
80	566.0
81	566.0
82	427.0
83	288.0
84	288.0
85	288.0
86	189.0
87	90.0
88	90.0
89	90.0
90	57.0
91	24.0
92	24.0
93	24.0
94	15.5
95	7.0
96	7.0
97	7.0
98	3.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.127668454843422
2	0.2590196535765581
3	0.319171137108555
4	0.355998576005696
5	0.35354341341255324
6	0.3277642061845546
7	0.32530904359141183
8	0.33635727526055414
9	0.3265366248879832
10	0.3572261573022673
11	0.2811161169148427
12	0.33144695007426866
13	0.30321258025312725
14	0.3044401615496986
15	0.33881243785369686
16	0.31057806803255544
17	0.3424951817434109
18	0.3228538809982691
19	0.32530904359141183
20	0.3019849989565559
21	0.33635727526055414
22	0.2897091859908422
23	0.27497821043198584
24	0.3044401615496986
25	0.2958470924736991
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	81461.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.04988890389266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.53043534406224	68.08779661433078
2	5.928879275556488	9.01781220461325
3	1.8207938532065666	4.154135107597501
4	0.8894126002808672	2.7055891776432897
5	0.43098577908346924	1.6388210309227726
6	0.3083081790447289	1.406808165870785
7	0.2066149053284047	1.0999128417279433
8	0.14366192636115638	0.8740378831588121
9	0.11944924214298397	0.8175691435165293
>10	0.6020887475585543	7.904395968623022
>50	0.012913431583025294	0.624838879954825
>100	0.004842536843634485	0.4652533114005475
>500	0.0016141789478781618	1.2030296706399382
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	980	1.2030296706399382	No Hit
GTATCAACGCAGAGTACTTTTTTTT	151	0.18536477578227617	No Hit
GTACATAAGCAGTGGTATCAACGCA	119	0.1460821742919925	No Hit
GGTATCAACGCAGAGTACTTTTTTT	109	0.13380636132627882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0012275812965713655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAAAA	40	0.005203212	14.257855	2
ATAAAAC	55	0.0030131193	12.097575	3
GTATCAA	130	4.1829022E-5	8.774065	1
CATGGGG	105	0.0043971003	8.1473465	4
TCAACGC	145	0.0012960908	7.21087	4
AACGCAG	150	0.00186807	6.966215	6
TATCAAC	155	0.0026223715	6.745652	2
ATCAACG	155	0.0026223715	6.745652	3
GTACATG	175	0.001293351	6.517877	1
CAACGCA	165	0.0050471025	6.3368244	5
>>END_MODULE