FastQCFastQC Report
Wed 25 May 2016
SRR1295170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81461
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGA36094.430340899326058No Hit
ATACACATCTCCGAGCCCACGAGAC16131.9800886313696124No Hit
ACACATCTCCGAGCCCACGAGACCT7210.8850861148279545No Hit
GTATCAACGCAGAGTACTTTTTTTT5910.725500546273677No Hit
GGTATCAACGCAGAGTACTTTTTTT4450.5462736769742577No Hit
TATCAACGCAGAGTACTTTTTTTTT3580.43947410417254884No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2040.25042658450055855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1950.23937835283141623No Hit
GTACATAAGCAGTGGTATCAACGCA1630.20009575134113258No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1430.17554412540970527No Hit
CACATCTCCGAGCCCACGAGACCTC1360.16695105633370572No Hit
TATACACATCTCCGAGCCCACGAGA1290.15835798725770614No Hit
TTATACACATCTCCGAGCCCACGAG1260.15467524336799204No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1220.14976491818170656No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1030.12644087354685066No Hit
GAGATGTGTATAAGAGACAGACACA970.11907538576742245No Hit
GCTTATGTACTCTGCGTTGATACCA850.10434441020856607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC3600.018.7312681
TTATACA3900.017.5339282
TATACAC4350.015.7200753
GGCATCT400.0052101514.25506812
CATCTGA400.005231458714.24631714
TTCTGTA400.005231458714.2463172
ATCTGAC400.005231458714.24631715
GAGACCT655.3428266E-513.15044719
ATACACA5350.012.9592664
TACACAT5400.012.8392735
GGGCATC550.003017674412.09520911
ACACATC6050.011.3098066
ATCTCCG6150.011.12590610
CACATCT6250.010.9478927
ACATCTC6300.010.8610038
GGTATCA1751.8189894E-1110.8543381
CATCTCC6400.010.6913019
TCTCCGA6400.010.69130111
CTCCGAG6600.010.36732212
CGAGCCC6600.010.36095815
Produced by FastQC (version 0.11.5)