##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295170_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81461
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.532917592467562	31.0	30.0	33.0	27.0	34.0
2	30.663532242422754	31.0	30.0	34.0	27.0	34.0
3	30.863910337462098	31.0	30.0	34.0	27.0	34.0
4	34.51025644173286	35.0	35.0	37.0	31.0	37.0
5	34.73678201838917	37.0	35.0	37.0	32.0	37.0
6	34.35733663961896	37.0	35.0	37.0	30.0	37.0
7	34.14145419280392	37.0	35.0	37.0	29.0	37.0
8	34.12623218472643	37.0	35.0	37.0	29.0	37.0
9	35.68391009194584	39.0	35.0	39.0	29.0	39.0
10	35.72802936374461	39.0	35.0	39.0	29.0	39.0
11	35.71592541216042	39.0	35.0	39.0	29.0	39.0
12	35.62947913725586	39.0	35.0	39.0	29.0	39.0
13	35.45668479395048	39.0	35.0	39.0	27.0	39.0
14	36.54365892881256	40.0	36.0	41.0	27.0	41.0
15	36.52750395894968	40.0	36.0	41.0	27.0	41.0
16	36.48628177901082	39.0	36.0	41.0	27.0	41.0
17	36.49485029646088	39.0	36.0	41.0	27.0	41.0
18	36.51144719559053	40.0	36.0	41.0	27.0	41.0
19	36.613778372472716	40.0	36.0	41.0	27.0	41.0
20	36.59191514958078	40.0	36.0	41.0	27.0	41.0
21	36.561336099483185	40.0	36.0	41.0	27.0	41.0
22	36.49584463731111	40.0	36.0	41.0	27.0	41.0
23	36.4576791348007	40.0	36.0	41.0	27.0	41.0
24	36.42421526865617	39.0	36.0	41.0	27.0	41.0
25	34.77745178674458	38.0	33.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.7865100697187088
1101	2	-0.09421359534843177
1101	3	-0.31006965600007064
1101	4	-0.1505967238787278
1101	5	-0.9416644524214774
1101	6	-0.566937459847189
1101	7	-0.41214528056433863
1101	8	-0.6585451012713932
1101	9	-0.917220417577127
1101	10	-0.9504602275461593
1101	11	-1.0936575587920316
1101	12	-0.9182774079770724
1101	13	-1.0888486548018363
1101	14	0.11365672790564219
1101	15	-0.799555183095805
1101	16	0.02696301230382403
1101	17	-0.2622927195155498
1101	18	-0.02318011422789823
1101	19	-0.3564907811567295
1101	20	0.09493514307612116
1101	21	-0.36069805581270487
1101	22	-0.40470485397126055
1101	23	-0.7633131130002226
1101	24	-0.3055390088017518
1101	25	-0.5944873589879194
1102	1	-0.08433615667523142
1102	2	-0.1956628707107484
1102	3	-0.06840298933340705
1102	4	-0.6303068688062652
1102	5	-0.5242731480736467
1102	6	-1.1013577497022666
1102	7	-0.7346090486802836
1102	8	-0.4618059708366147
1102	9	-1.0838870842437913
1102	10	-0.7653153000099238
1102	11	-0.6947445153137721
1102	12	-1.27262523406403
1102	13	-1.0218196692945867
1102	14	-1.217865011224795
1102	15	-0.6452073570088501
1102	16	-1.3368051036382056
1102	17	-0.8999738789358389
1102	18	-1.1778902591554328
1102	19	-1.1858386072436815
1102	20	-1.345282248228223
1102	21	-1.5045386355228487
1102	22	-0.6018063032466259
1102	23	-1.3698348521306585
1102	24	-1.7450317624249365
1102	25	-1.2636902575386415
1103	1	0.08549855406857176
1103	2	-0.02651686520110985
1103	3	0.04034356714042531
1103	4	0.1561945086042016
1103	5	-0.13749628856951546
1103	6	0.1193722778459474
1103	7	0.13798048300015608
1103	8	0.4499708886675222
1103	9	-0.006476615924228213
1103	10	0.11119985150522638
1103	11	0.4042224268349912
1103	12	0.5343995593243989
1103	13	0.13141724530596122
1103	14	0.5058953193537192
1103	15	0.45520586613164227
1103	16	-0.1976866463379281
1103	17	-0.03771492576779423
1103	18	0.4885697959409825
1103	19	0.31436800432656753
1103	20	0.007403702184994643
1103	21	0.560681750152078
1103	22	0.26651325598202646
1103	23	0.8603304371255405
1103	24	0.6292795323409024
1103	25	0.5248083650508875
1104	1	0.4063418094264648
1104	2	0.4659190501932038
1104	3	0.45433712366094525
1104	4	0.1719530181993818
1104	5	0.38589634345177615
1104	6	0.6239247361734428
1104	7	0.3148259795683046
1104	8	0.49031267323118755
1104	9	0.477694836660163
1104	10	0.30149260959458957
1104	11	0.5599165016353851
1104	12	0.5044934100037679
1104	13	0.8927283533042782
1104	14	0.8486604125040245
1104	15	0.6403858633301311
1104	16	0.25076551783072176
1104	17	0.8401391154144449
1104	18	0.46377640751123295
1104	19	0.576449528349535
1104	20	1.0076838534666877
1104	21	0.6159415904658516
1104	22	0.6682501939285217
1104	23	0.6242329444795089
1104	24	0.4279908364451188
1104	25	0.8395583300319771
1105	1	0.29472367238459896
1105	2	0.723995248947368
1105	3	0.7565970106665958
1105	4	0.7842230457236496
1105	5	0.532137108336606
1105	6	1.2052661819216652
1105	7	0.6606900966188647
1105	8	0.47857864454800136
1105	9	0.889617189260484
1105	10	1.0622488025541728
1105	11	0.9383751428058886
1105	12	1.13805852661973
1105	13	0.9044623819874644
1105	14	1.3571349887752078
1105	15	0.9321430703415743
1105	16	1.5392632724301762
1105	17	0.7666927877308325
1105	18	0.7372806810155055
1105	19	1.1767682303631517
1105	20	1.1502305722845918
1105	21	1.1734528174686005
1105	22	1.0887919873516694
1105	23	1.6681993359035303
1105	24	1.5295408871477107
1105	25	1.631822562974179
1106	1	0.1380776364282177
1106	2	0.1330727614731586
1106	3	-0.07386275944834964
1106	4	-0.3343298573120137
1106	5	-0.3277214239357207
1106	6	0.11559627328624344
1106	7	0.38320704327374244
1106	8	0.27698713261166574
1106	9	-0.22584110723229855
1106	10	0.7585352747027159
1106	11	0.5992210019276101
1106	12	0.5756506280049365
1106	13	0.1491573421996648
1106	14	0.5726522301545174
1106	15	0.34962022919804525
1106	16	0.16032133314340768
1106	17	0.5454284199147352
1106	18	0.7482591661319233
1106	19	0.0805407031011427
1106	20	0.3555798207372902
1106	21	-0.09563058954584136
1106	22	0.19761898410970247
1106	23	0.531889285800375
1106	24	0.7707728352762118
1106	25	1.2567120413119284
1107	1	0.6870378891262945
1107	2	0.30153814710095617
1107	3	0.6013807256793164
1107	4	0.437123156639025
1107	5	0.4742001343690987
1107	6	0.8359832172188533
1107	7	0.35254107854618155
1107	8	0.48914822763666876
1107	9	0.43545133814807
1107	10	0.8655371172928739
1107	11	1.3107898358312653
1107	12	1.1330999567756663
1107	13	0.8099233332499409
1107	14	0.7998830803782582
1107	15	1.138380429250688
1107	16	0.8324060922905474
1107	17	1.2654205230997846
1107	18	1.0744634304119955
1107	19	0.7971766599318926
1107	20	0.8869696601687238
1107	21	1.0207158174033566
1107	22	0.6251657069314973
1107	23	0.6404704913807961
1107	24	0.775642537829512
1107	25	0.6942613964053734
1108	1	0.02035134332476929
1108	2	-0.2758712040440834
1108	3	0.04305534399992794
1108	4	0.012922297860399112
1108	5	0.04916435192635049
1108	6	-0.05708691636893093
1108	7	0.20809928465305205
1108	8	0.5585065291633882
1108	9	0.23590458242287582
1108	10	0.5898930333234063
1108	11	0.038067984686229295
1108	12	0.3461247659359685
1108	13	0.3323469973720776
1108	14	0.4352599887752078
1108	15	-0.034269857008851545
1108	16	0.012153229695130108
1108	17	-0.3869530456025032
1108	18	0.46377640751123295
1108	19	0.47978639275631707
1108	20	0.3765927517717742
1108	21	0.660565531143817
1108	22	0.2695478634200441
1108	23	0.21454014786934295
1108	24	1.0466349042417278
1108	25	0.019643075794689935
1109	1	0.11920715041138408
1109	2	0.16864159123150557
1109	3	0.2946547534487465
1109	4	0.4339975931359916
1109	5	0.4883252771232023
1109	6	0.6620936938672912
1109	7	0.3290392452829707
1109	8	0.516737336250003
1109	9	0.3615197399031942
1109	10	0.18278181285096196
1109	11	0.2723814689381996
1109	12	0.40099681318006475
1109	13	0.22850841469491456
1109	14	0.34926097302717807
1109	15	0.5284146902352447
1109	16	0.039835316309300595
1109	17	0.03178465124788943
1109	18	0.6527527854639885
1109	19	0.5911298966933245
1109	20	0.7234185391733519
1109	21	0.6863406295690169
1109	22	0.1262776862546886
1109	23	0.6927635730661947
1109	24	0.5466349042417278
1109	25	0.8454304773694901
1110	1	-0.24966462382851518
1110	2	-0.5100181018543033
1110	3	0.07533180385394189
1110	4	-0.02568399776004071
1110	5	0.4071137132402214
1110	6	0.17631864932450014
1110	7	-0.28266257666081884
1110	8	-0.625857065727125
1110	9	-0.5274393956793162
1110	10	-0.6996826966036025
1110	11	-0.4277737123940639
1110	12	-0.22116538004943465
1110	13	-0.10789022890529765
1110	14	-0.23410588713720415
1110	15	-0.509805897154834
1110	16	-0.29179293818808105
1110	17	0.16328646169676375
1110	18	-0.5508221326347496
1110	19	-0.04769992111229726
1110	20	-0.37101217523552776
1110	21	-0.262507005352532
1110	22	0.16559029042734608
1110	23	-0.19136769884598692
1110	24	0.14517505022713095
1110	25	-0.02324013588414431
1111	1	-0.3144780006468615
1111	2	-0.3672940763845105
1111	3	-0.158296606354682
1111	4	0.13919667729302176
1111	5	0.24735805760010976
1111	6	0.03250749852468715
1111	7	0.026029249192056625
1111	8	-0.2644655453046951
1111	9	0.013276036323581764
1111	10	0.2859258347418461
1111	11	0.08876612298410436
1111	12	-0.07652594328389029
1111	13	-0.0971742792236654
1111	14	0.18692222281776338
1111	15	-0.6866967187109765
1111	16	-0.22687602562401565
1111	17	0.1851325040428904
1111	18	0.059521088362295416
1111	19	0.4455443714797198
1111	20	0.4502851276582973
1111	21	0.3800358325622568
1111	22	-0.2018063032466202
1111	23	0.31704458049345874
1111	24	-0.26896793263770746
1111	25	-0.2667044419358078
1112	1	0.13689672003709674
1112	2	0.0038805082846842254
1112	3	-0.51280938202747
1112	4	-0.09377718844097416
1112	5	-0.23797177821063542
1112	6	-0.009462772533311181
1112	7	0.33502565451606614
1112	8	0.19881046751955012
1112	9	0.22798506187493217
1112	10	0.3204152935973781
1112	11	-0.01426506325897492
1112	12	-0.12262523406403147
1112	13	-0.0075502629018942
1112	14	0.02836786548753878
1112	15	-0.3171251652280276
1112	16	-0.25689642783911637
1112	17	0.04294849549338409
1112	18	0.2514476403879442
1112	19	0.1473805708385072
1112	20	-0.39630964548850045
1112	21	-0.4073925168013872
1112	22	0.2936274867077131
1112	23	0.302082956088519
1112	24	-0.316378794388406
1112	25	0.21998554154811245
1113	1	0.5489971766581014
1113	2	0.14600379595591662
1113	3	0.2913192328888172
1113	4	0.6196931311937348
1113	5	0.0048935185930147895
1113	6	0.2250311391866262
1113	7	-0.08877571534694795
1113	8	0.28680514027450243
1113	9	-0.1894426397993456
1113	10	0.3416291444345205
1113	11	0.07192215135289359
1113	12	-0.3240141229529172
1113	13	-0.03570855818347951
1113	14	0.08630165544187207
1113	15	-0.21742957923107298
1113	16	-0.05902732586042703
1113	17	0.5375261210641611
1113	18	0.4151652964001187
1113	19	0.4988836149785385
1113	20	0.5797177517717742
1113	21	0.29685025336603843
1113	22	0.3606936967533798
1113	23	0.16766514786934295
1113	24	-0.5505873179804937
1113	25	-0.16785692420531007
1114	1	0.24188761954854598
1114	2	-0.06466053970842012
1114	3	0.18783244190202453
1114	4	0.3335625950631993
1114	5	0.15684573304523042
1114	6	-0.23917826252277763
1114	7	0.06550750143627226
1114	8	-0.934008768039412
1114	9	-0.09484279519950434
1114	10	-0.720967980662607
1114	11	-0.39107318664244417
1114	12	-0.14010775154655164
1114	13	-0.17246069493561578
1114	14	-0.16734053570031904
1114	15	0.13416327236177494
1114	16	-0.3845906514237498
1114	17	-0.44775942672138314
1114	18	0.21902116275598615
1114	19	-0.5979015443066231
1114	20	-0.019932597878572267
1114	21	0.21230285631864376
1114	22	0.038286936846617436
1114	23	0.05359172129591627
1114	24	-0.0967217391149191
1114	25	-0.7644478332962166
1115	1	0.9989971766581043
1115	2	0.8932260181781402
1115	3	0.7788192328888179
1115	4	0.9215449830455853
1115	5	0.8164675926670881
1115	6	1.0810496577051438
1115	7	1.2116872476160125
1115	8	1.2928236587930186
1115	9	1.5420388416821353
1115	10	1.1152402555456291
1115	11	1.2432184476491912
1115	12	1.081078469639671
1115	13	1.1642914418165233
1115	14	1.364542396182614
1115	15	1.2751630133615208
1115	16	1.3706023037692034
1115	17	1.0703964914345363
1115	18	0.5452578889927153
1115	19	0.6502725038674271
1115	20	1.566754788808808
1115	21	1.2686095126252965
1115	22	1.3981936967533741
1115	23	1.2875725552767534
1115	24	1.242931200538024
1115	25	1.1890875202391342
1116	1	0.011882330719728884
1116	2	0.08612984637608534
1116	3	0.48488832719320385
1116	4	0.2926623188687998
1116	5	0.24211340654819935
1116	6	0.05235373489157524
1116	7	-1.5211145267812753E-5
1116	8	-0.21243622293745545
1116	9	-0.17940529152670592
1116	10	0.24259786525617955
1116	11	-0.25755964136418896
1116	12	-0.45455800717327577
1116	13	-0.7322538429640559
1116	14	-0.15547005324160068
1116	15	-0.04142584440381114
1116	16	-0.37742135013679956
1116	17	-0.0876489489638459
1116	18	0.009994894906192542
1116	19	0.16269080452102003
1116	20	0.010390020679338363
1116	21	0.1014137454295323
1116	22	-0.047744678596764345
1116	23	-0.519834852130657
1116	24	-0.5373987092036501
1116	25	-2.502415747734723
1201	1	-0.378598451757199
1201	2	0.3365775664477191
1201	3	0.18009427842615722
1201	4	-0.16855823492648625
1201	5	-0.4455846234834837
1201	6	0.3282870590409033
1201	7	-0.3070134202649797
1201	8	0.035325176704368744
1201	9	0.5532713857015636
1201	10	0.3585918038152087
1201	11	0.18189482894852205
1201	12	1.065899356099905
1201	13	1.0526338826179824
1201	14	0.7997579395948833
1201	15	0.5949565774173777
1201	16	1.1522659346131618
1201	17	0.654670929807331
1201	18	0.8654157517735257
1201	19	0.1342433599694317
1201	20	0.2477505386570229
1201	21	0.7969640967175877
1201	22	0.5632210191577514
1201	23	0.42278809868901845
1201	24	0.7351594944056643
1201	25	0.7348070102209192
1202	1	0.35822582679584514
1202	2	0.46935090339393426
1202	3	0.2717485258181114
1202	4	0.1479300457942827
1202	5	0.03605743043874554
1202	6	0.21854583156495266
1202	7	0.6916994912646217
1202	8	0.7144336985848767
1202	9	0.9026142931666783
1202	10	0.4169849754721682
1202	11	0.3215777987358166
1202	12	0.641837575853323
1202	13	0.7760453444795132
1202	14	0.37366391439504554
1202	15	0.2651232215035435
1202	16	0.3147100478769502
1202	17	0.17716110729006118
1202	18	0.984437564536023
1202	19	1.3639547811860666
1202	20	1.5032714707800352
1202	21	1.1970453865157182
1202	22	0.4565959006101252
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2211	12	0.6855939440181587
2211	13	0.6910798740844086
2211	14	0.7475459476793205
2211	15	1.2102720950459442
2211	16	0.6540624762704752
2211	17	0.6319895913837996
2211	18	0.5596668184701343
2211	19	0.7090244064549438
2211	20	0.3434163819087601
2211	21	0.4487718667602536
2211	22	0.7730795415022342
2211	23	0.23358980540358942
2211	24	0.50553901383077
2211	25	0.8706704730549646
2212	1	0.2272532472982789
2212	2	0.16504353105525382
2212	3	0.20913564201317314
2212	4	0.2913266852776175
2212	5	0.22936923603231207
2212	6	-0.04203103888549009
2212	7	-0.3102161126979439
2212	8	-0.0639582887174086
2212	9	-0.18079657651752257
2212	10	0.49169353001214944
2212	11	0.15442767011668934
2212	12	0.3376396665982213
2212	13	0.34103905035220805
2212	14	0.204817107980503
2212	15	0.08095158338849728
2212	16	0.2581176336686397
2212	17	-0.4937928634833
2212	18	-0.29781299646227666
2212	19	-0.3363352959854069
2212	20	-0.7563749634600114
2212	21	-0.3347262734919454
2212	22	-0.7470601663812815
2212	23	-0.3807620044485347
2212	24	-0.6983982083410538
2212	25	-1.2195953348013333
2213	1	0.07350698057967264
2213	2	-0.17997659620094453
2213	3	-0.15189972135954832
2213	4	0.2226343076643218
2213	5	0.43258959702438915
2213	6	0.3978252568336842
2213	7	0.9598353957641663
2213	8	0.660579650078418
2213	9	0.923302458239867
2213	10	0.4433448307090302
2213	11	0.7508437199803453
2213	12	0.5277015633215925
2213	13	0.715925428744626
2213	14	0.44210230903664893
2213	15	0.24989068220683208
2213	16	0.8494694061657171
2213	17	0.9235555328288712
2213	18	1.0846914401909729
2213	19	0.9535404777236351
2213	20	1.427756967458052
2213	21	0.9949711683987204
2213	22	0.615187160805668
2213	23	0.34291024590855557
2213	24	0.8015368650260442
2213	25	0.9354927489973051
2214	1	0.20852098618191306
2214	2	0.006718081670200604
2214	3	-0.23864108457150124
2214	4	-0.320783059282455
2214	5	-0.21713029093078973
2214	6	0.05697558363107191
2214	7	-0.5512757153469465
2214	8	-1.2296631136937535
2214	9	-0.8315061318628381
2214	10	-0.3284105381051674
2214	11	-0.6126016581709166
2214	12	-0.27976809120688984
2214	13	-0.10872443119934871
2214	14	-1.007150725510506
2214	15	-0.49520735700885155
2214	16	-0.40347177030486847
2214	17	-0.573783402745363
2214	18	-0.871937878203056
2214	19	-0.7394100358151121
2214	20	-0.7917108196567995
2214	21	-0.34858625457047054
2214	22	-0.7065682080085267
2214	23	-1.1555491378449432
2214	24	-0.710507952901132
2214	25	-1.1410712099195948
2215	1	0.4378860655469907
2215	2	0.5140593515114737
2215	3	0.3954858995554851
2215	4	0.3349709089715134
2215	5	0.11600462970412906
2215	6	0.4125311391866262
2215	7	0.057057617986387754
2215	8	0.34236069583005246
2215	9	0.2688906935339901
2215	10	0.33468469999007056
2215	11	0.0927554846862293
2215	12	0.7662636548248543
2215	13	0.2351247751498562
2215	14	0.3154683221085435
2215	15	0.6228481985467056
2215	16	0.6631948963617944
2215	17	0.3430816766197182
2215	18	0.33877640751123295
2215	19	0.6308280594229814
2215	20	0.4963844184384385
2215	21	0.6510169200327027
2215	22	0.9440270300867084
2215	23	1.2787762589804572
2215	24	0.3799682375750635
2215	25	0.6168652980169114
2216	1	-0.00841023074930547
2216	2	-0.8401073151551941
2216	3	-0.04340298933340492
2216	4	-0.1006772391766404
2216	5	0.327578703778201
2216	6	-0.4596910830356009
2216	7	0.3524279883567587
2216	8	0.3298606958300567
2216	9	-0.5172204175771213
2216	10	-0.6403153000099238
2216	11	0.04321844764919547
2216	12	-0.5263289377677367
2216	13	-0.4579307804056967
2216	14	0.016394248034465875
2216	15	0.45294079113929797
2216	16	0.35578748895439105
2216	17	0.440766861804903
2216	18	-0.20289025915543135
2216	19	-0.03861638502146292
2216	20	-0.3369489148948901
2216	21	-0.5239830799672944
2216	22	-0.572176673616994
2216	23	-0.23835337064917894
2216	24	0.11700527461209731
2216	25	-0.8775791464275358
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	9.0
10	15.0
11	15.0
12	63.0
13	146.0
14	233.0
15	336.0
16	455.0
17	515.0
18	520.0
19	606.0
20	601.0
21	557.0
22	589.0
23	631.0
24	629.0
25	693.0
26	749.0
27	822.0
28	977.0
29	1067.0
30	1410.0
31	1710.0
32	2312.0
33	3073.0
34	4219.0
35	5814.0
36	8733.0
37	13800.0
38	30157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.71692553256799	20.666707594081895	11.427343606114555	29.18902326723556
2	14.675734400510674	19.962927044843546	40.947201728434464	24.414136826211315
3	16.684262214583846	26.090105573287502	32.074637859071935	25.150994353056717
4	11.685346362062827	21.120536207510344	32.47934594468519	34.71477148574164
5	12.422802558719168	37.18860117622503	36.25425123086179	14.134345034194014
6	29.765865756485653	38.28653513241415	15.822170929047624	16.125428182052573
7	24.720108028480237	32.31524674686963	19.52000982077093	23.444635403879204
8	23.435754532843937	35.98777329028615	21.537913848344605	19.03855832852531
9	23.59902284528793	14.873374989258664	18.877745178674456	42.64985698677895
10	14.95071261094266	30.523808939246997	31.127778937160112	23.397699512650224
11	30.946856700917024	22.617513104752085	25.15007549810334	21.28555469622755
12	21.01696579832552	25.69299516315156	26.445530211888336	26.844508826634584
13	28.56265884172918	20.770052425444145	28.248351729303	22.418937003523677
14	20.978454361303786	23.0004296851022	24.33490884537475	31.68620710821926
15	24.829059304452436	26.757589521366054	21.70363732338174	26.709713850799773
16	26.11924724714894	26.784596309890624	22.902984250131965	24.19317219282847
17	19.12326143798873	32.46093222523662	24.21895140005647	24.196854936718186
18	24.037859536699443	24.97943750843983	26.714052468112794	24.268650486747934
19	21.64839616503603	26.639741716895198	26.45928726629921	25.25257485176956
20	21.342990424748344	25.56469432850479	25.98821507488338	27.104100171863493
21	24.711514853916032	24.990179229069483	25.419837957279647	24.87846795973484
22	20.840955128598612	32.250936099686946	25.55153152047143	21.356577251243017
23	23.056902584248974	24.38278804247744	26.183782456571116	26.376526916702474
24	25.728000392850127	26.109801610685523	25.958799842859946	22.2033981536044
25	21.295455103493996	28.9365777002971	27.01033712279323	22.75763007341567
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	220.0
2	145.5
3	71.0
4	71.0
5	71.0
6	150.0
7	229.0
8	229.0
9	229.0
10	272.5
11	316.0
12	316.0
13	316.0
14	383.5
15	451.0
16	451.0
17	451.0
18	773.5
19	1096.0
20	1096.0
21	1096.0
22	1657.0
23	2218.0
24	2218.0
25	2218.0
26	2953.0
27	3688.0
28	3688.0
29	3688.0
30	4349.5
31	5011.0
32	5011.0
33	5011.0
34	5635.5
35	6260.0
36	6260.0
37	6260.0
38	6774.0
39	7288.0
40	7288.0
41	7288.0
42	7671.5
43	8055.0
44	8055.0
45	8055.0
46	8074.0
47	8093.0
48	8093.0
49	8093.0
50	9958.0
51	11823.0
52	11823.0
53	11823.0
54	10481.0
55	9139.0
56	9139.0
57	9139.0
58	7919.5
59	6700.0
60	6700.0
61	6700.0
62	5795.0
63	4890.0
64	4890.0
65	4890.0
66	4060.5
67	3231.0
68	3231.0
69	3231.0
70	2409.5
71	1588.0
72	1588.0
73	1588.0
74	1108.0
75	628.0
76	628.0
77	628.0
78	463.5
79	299.0
80	299.0
81	299.0
82	214.0
83	129.0
84	129.0
85	129.0
86	81.5
87	34.0
88	34.0
89	34.0
90	19.0
91	4.0
92	4.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019641300745141844
2	0.0
3	0.0012275812965713653
4	0.0
5	0.017186138151999115
6	0.014730975558856387
7	0.0012275812965713653
8	0.0
9	0.0
10	0.0
11	0.0024551625931427306
12	0.0036827438897140967
13	0.014730975558856387
14	0.007365487779428193
15	0.0
16	0.0
17	0.0
18	0.0024551625931427306
19	0.0
20	0.0012275812965713653
21	0.0012275812965713653
22	0.007365487779428193
23	0.007365487779428193
24	0.006137906482856828
25	0.008593069075999557
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	81461.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.51089478400708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.48564471151958	53.437841421048105
2	7.988688582537999	9.987601428904629
3	2.6412945288873177	4.953290531665459
4	1.1802364400455598	2.9511054369575627
5	0.6500137465142768	2.03164704582561
6	0.4241781548250266	1.5909453603564896
7	0.29456816307293504	1.2889603613999336
8	0.20227013864341542	1.0115269883748053
9	0.20227013864341542	1.1379678619216558
>10	0.8719217626958878	9.742085169590355
>50	0.029456816307293507	1.2386295282405078
>100	0.021601665292015237	2.6073826739175803
>500	0.003927575507639135	1.6105866611016313
>1k	0.003927575507639135	6.41042953069567
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGA	3609	4.430340899326058	No Hit
ATACACATCTCCGAGCCCACGAGAC	1613	1.9800886313696124	No Hit
ACACATCTCCGAGCCCACGAGACCT	721	0.8850861148279545	No Hit
GTATCAACGCAGAGTACTTTTTTTT	591	0.725500546273677	No Hit
GGTATCAACGCAGAGTACTTTTTTT	445	0.5462736769742577	No Hit
TATCAACGCAGAGTACTTTTTTTTT	358	0.43947410417254884	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	204	0.25042658450055855	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	195	0.23937835283141623	No Hit
GTACATAAGCAGTGGTATCAACGCA	163	0.20009575134113258	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	143	0.17554412540970527	No Hit
CACATCTCCGAGCCCACGAGACCTC	136	0.16695105633370572	No Hit
TATACACATCTCCGAGCCCACGAGA	129	0.15835798725770614	No Hit
TTATACACATCTCCGAGCCCACGAG	126	0.15467524336799204	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	122	0.14976491818170656	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	103	0.12644087354685066	No Hit
GAGATGTGTATAAGAGACAGACACA	97	0.11907538576742245	No Hit
GCTTATGTACTCTGCGTTGATACCA	85	0.10434441020856607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	360	0.0	18.731268	1
TTATACA	390	0.0	17.533928	2
TATACAC	435	0.0	15.720075	3
GGCATCT	40	0.00521015	14.255068	12
CATCTGA	40	0.0052314587	14.246317	14
TTCTGTA	40	0.0052314587	14.246317	2
ATCTGAC	40	0.0052314587	14.246317	15
GAGACCT	65	5.3428266E-5	13.150447	19
ATACACA	535	0.0	12.959266	4
TACACAT	540	0.0	12.839273	5
GGGCATC	55	0.0030176744	12.095209	11
ACACATC	605	0.0	11.309806	6
ATCTCCG	615	0.0	11.125906	10
CACATCT	625	0.0	10.947892	7
ACATCTC	630	0.0	10.861003	8
GGTATCA	175	1.8189894E-11	10.854338	1
CATCTCC	640	0.0	10.691301	9
TCTCCGA	640	0.0	10.691301	11
CTCCGAG	660	0.0	10.367322	12
CGAGCCC	660	0.0	10.360958	15
>>END_MODULE