FastQCFastQC Report
Wed 25 May 2016
SRR1295169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163948
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA48152.9369068241149634No Hit
GTACATAAGCAGTGGTATCAACGCA22601.3784858613706785No Hit
GTATCAACGCAGAGTACTTTTTTTT16070.9801888403640179No Hit
GCTTATGTACTCTGCGTTGATACCA13400.8173323248834996No Hit
GGTATCAACGCAGAGTACTTTTTTT12620.7697562641813258No Hit
TATCAACGCAGAGTACTTTTTTTTT10120.6172688901358967No Hit
ATACAGGGTGACAGCCCCGTACACA10010.6105594456778979No Hit
GTACTGGTTCACTATCGGTCAGTCA9930.6056798497084441No Hit
CTTATGTACTCTGCGTTGATACCAC9540.5818918193573572No Hit
GGATACCACGTGTCCCGCCCTACTC9210.5617634859833606No Hit
GTGGTATCAACGCAGAGTACATGGG8390.5117476272964598No Hit
ACCCTGTATCGCGCGCCTTTCCAGA6590.40195671798375093No Hit
GAGTACATAAGCAGTGGTATCAACG6450.3934174250372069No Hit
TATGTACTCTGCGTTGATACCACTG5800.3537707077853953No Hit
GGTATCAACGCAGAGTACATGGGGG5320.32449313196867297No Hit
GTTCACTATCGGTCAGTCAGGAGTA5070.30924439456413005No Hit
GTATTTAGCCTTGGAGGATGGTCCC5010.3055846975870398No Hit
CCCATATTCAGACAGGATACCACGT4740.28911606119013344No Hit
ATCATTAACTGAATCCATAGGTTAA4620.28179666723595287No Hit
GCGTTGATACCACTGCTTATGTACT4460.2720374752970454No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4190.25556883890013904No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4130.25190914192304875No Hit
CATAAGCAGTGGTATCAACGCAGAG4110.25068924293068534No Hit
TATTCAGACAGGATACCACGTGTCC3910.238490253007051No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG3880.2366604045185059No Hit
CTGCTTATGTACTCTGCGTTGATAC3830.23361065703759726No Hit
GCCTTGGAGGATGGTCCCCCCATAT3810.23239075804523385No Hit
GTTAATGATAGTGTGTCGAAACACA3750.22873106106814353No Hit
CTATCGGTCAGTCAGGAGTATTTAG3700.22568131358723498No Hit
GGTTAATGAGGCGAACCGGGGGAAC3650.2226315661063264No Hit
GCCCAGAGCCTGAATCAGTGTGTGT3650.2226315661063264No Hit
ACGCAGAGTACATAAGCAGTGGTAT3520.21470222265596411No Hit
GTATCAACGCAGAGTACATGGGGGT3450.21043257618269207No Hit
TTCCAGACGCTTCCACTAACACACA3370.20555298021323834No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3150.1921340912972406No Hit
CATCTAAGTACCCCGAGGAAAAGAA3100.189084343816332No Hit
ACTCTGCGTTGATACCACTGCTTAT3080.18786444482396858No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3070.18725449532778687No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3060.18664454583160514No Hit
GGTATCAACGCAGAGTACATAAGCA3050.18603459633542344No Hit
GGTATCAACGCAGAGTACATGGGGA3050.18603459633542344No Hit
GTCCCGCCCTACTCATCGAGCTCAC2990.18237489935833312No Hit
CACACACACTGATTCAGGCTCTGGG2970.1811550003659697No Hit
TCACGGTACTGGTTCACTATCGGTC2960.18054505086978798No Hit
GTACATGGGGGTTAAGCGACTAAGC2890.17627540439651596No Hit
GTATCAACGCAGAGTACATAAGCAG2850.1738356064117891No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2830.17261570741942567No Hit
CGGTACTGGTTCACTATCGGTCAGT2810.17139580842706223No Hit
GTGGATGCCCTGGCAGTCAGAGGCG2730.1665162124576085No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2680.16346646497669992No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2670.16285651548051822No Hit
GCCTGAATCAGTGTGTGTGTTAGTG2670.16285651548051822No Hit
AGCGTACACGGTGGATGCCCTGGCA2650.16163661648815478No Hit
TATCAACGCAGAGTACATAAGCAGT2650.16163661648815478No Hit
GGGTTTCCCCATTCGGAAATCGCCG2520.15370727303779247No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2470.1506575255568839No Hit
GTTATAACCGGCGATTTCCGAATGG2440.14882767706833874No Hit
AGCTCACAGCATGTGCATTTTTGTG2440.14882767706833874No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2420.14760777807597533No Hit
GGTATCAACGCAGAGTACATGGGCA2400.1463878790836119No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2390.14577792958743016No Hit
GGAGTATTTAGCCTTGGAGGATGGT2390.14577792958743016No Hit
GGTTCACTATCGGTCAGTCAGGAGT2330.14211823261033987No Hit
ACGCAGAGTACATGGGGGTTAAGCG2320.14150828311415817No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2260.13784858613706785No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2250.13723863664088615No Hit
TCATTAACCTATGGATTCAGTTAAT2250.13723863664088615No Hit
CTACTGGGGGAATCTCGGTTGATTT2240.13662868714470444No Hit
GTACTTAGATGTTTCAGTTCCCCCG2180.13296899016761413No Hit
CCCATGTACTCTGCGTTGATACCAC2180.13296899016761413No Hit
GTATCGCGCGCCTTTCCAGACGCTT2180.13296899016761413No Hit
GGGTGACAGCCCCGTACACAAAAAT2150.13113914167906898No Hit
GCCCTACTCATCGAGCTCACAGCAT2150.13113914167906898No Hit
TTTGTGTACGGGGCTGTCACCCTGT2130.12991924268670554No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2110.1286993436943421No Hit
GTGGTATCAACGCAGAGTACATAAG2080.12686949520579696No Hit
CCTATGGATTCAGTTAATGATAGTG2030.12381974772488838No Hit
ACATAAGCAGTGGTATCAACGCAGA2020.12320979822870667No Hit
GTATCAACGCAGAGTACATGGGGGG2010.12259984873252494No Hit
GTCCCCCCATATTCAGACAGGATAC1990.12137994974016152No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1970.12016005074779808No Hit
GTACATGGGCATCTAAGTACCCCGA1940.11833020225925295No Hit
GTGCTAATCTGCGATAAGCGTCGGT1870.11406055578598091No Hit
GGTATCAACGCAGAGTACATGGGTA1850.11284065679361749No Hit
AAGCAGTGGTATCAACGCAGAGTAC1850.11284065679361749No Hit
GTATCAACGCAGAGTACATGGGGAA1800.10979090931270892No Hit
CTCCTATACTGGATGCTATTTCCTG1790.10918095981652719No Hit
CACTATCATTAACTGAATCCATAGG1790.10918095981652719No Hit
CACTGATTCAGGCTCTGGGCTGCTC1780.10857101032034548No Hit
GTGGTATCAACGCAGAGTACTTTTT1720.10491131334325518No Hit
AACACACACACTGATTCAGGCTCTG1700.10369141435089176No Hit
GGGCTGTCACCCTGTATCGCGCGCC1690.10308146485471002No Hit
GATACCACTGCTTATGTACTCTGCG1680.10247151535852832No Hit
GTACGGGGCTGTCACCCTGTATCGC1660.10125161636616489No Hit
ATGATAGTGTGTCGAAACACACTGG1650.10064166686998316No Hit
GATATGAACCGTTATAACCGGCGAT1650.10064166686998316No Hit
GTATCAACGCAGAGTACATGGGCAT1650.10064166686998316No Hit
CTGTCACCCTGTATCGCGCGCCTTT1640.10003171737380145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA250.005978636419.0168971
GAATCTT250.006010557518.9993866
CTGTCCT402.7428055E-416.62446410
GGTTATA350.002146211516.3001981
CCTCCCT350.002163906616.28019114
TCCCTTT350.002163906616.28019116
ATAAGGA551.1195896E-515.54018316
GATAAGG551.1195896E-515.54018315
GGATAAG508.6629436E-515.19484414
TAAGGAA508.6629436E-515.19484417
TCCTCCC400.005264150414.24516713
TGTCCTC400.005264150414.24516711
GTCCTCC400.005264150414.24516712
CCCTTTT400.005264150414.24516717
AGGAATT602.5560808E-514.24079719
AATGGAT551.9488625E-413.81349611
TGGATAA551.9488625E-413.81349613
TGCTCTG500.001479567513.3118285
GGGAATG604.0583027E-412.6662578
GGAATGG604.0583027E-412.6662579
Produced by FastQC (version 0.11.5)