FastQCFastQC Report
Wed 25 May 2016
SRR1295169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163948
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52463.199795056969283No Hit
GGTATCAACGCAGAGTACTTTTTTT37772.3037792470783423No Hit
TATCAACGCAGAGTACTTTTTTTTT32972.0110034889111184No Hit
GTACATAAGCAGTGGTATCAACGCA23741.4480201039353942No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17841.0881499011881817No Hit
GCTTATGTACTCTGCGTTGATACCA14550.8874765169443971No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13900.8478297996925855No Hit
ATACAGGGTGACAGCCCCGTACACA11030.6727742942884329No Hit
CTTATGTACTCTGCGTTGATACCAC10960.6685046478151608No Hit
GTACTGGTTCACTATCGGTCAGTCA10670.6508161124258911No Hit
GTGGTATCAACGCAGAGTACATGGG9620.586771415326811No Hit
GGATACCACGTGTCCCGCCCTACTC9600.5855515163344476No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8900.5428550516017274No Hit
ACCCTGTATCGCGCGCCTTTCCAGA7480.45624222314392365No Hit
GAGTACATAAGCAGTGGTATCAACG7290.4446531827164711No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7220.4403835362431991No Hit
GGTATCAACGCAGAGTACATGGGGG5790.35316075828921367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5720.3488911118159416No Hit
GTGGTATCAACGCAGAGTACTTTTT5670.34584136433503304No Hit
CCCATATTCAGACAGGATACCACGT5520.3366921218923073No Hit
GCGTTGATACCACTGCTTATGTACT5290.32266328348012785No Hit
ATCATTAACTGAATCCATAGGTTAA5140.3135140410374021No Hit
ATCAACGCAGAGTACTTTTTTTTTT5080.3098543440603118No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4950.3019250006099495No Hit
CATAAGCAGTGGTATCAACGCAGAG4910.2994852026252226No Hit
TATGTACTCTGCGTTGATACCACTG4800.2927757581672238No Hit
GTTCACTATCGGTCAGTCAGGAGTA4520.2756971722741357No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4510.27508722277795394No Hit
GTATTTAGCCTTGGAGGATGGTCCC4440.270817576304682No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4380.26715787932759166No Hit
CTGCTTATGTACTCTGCGTTGATAC4280.26105838436577455No Hit
CTATCGGTCAGTCAGGAGTATTTAG4280.26105838436577455No Hit
GTTAATGATAGTGTGTCGAAACACA4250.2592285358772294No Hit
ACGCAGAGTACATAAGCAGTGGTAT4190.25556883890013904No Hit
GCCTTGGAGGATGGTCCCCCCATAT4050.24702954595359505No Hit
GTATCAACGCAGAGTACATGGGGGT4020.24519969746504988No Hit
CACACACACTGATTCAGGCTCTGGG3800.23178080854905214No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3710.22629126308341668No Hit
TATTCAGACAGGATACCACGTGTCC3700.22568131358723498No Hit
GTCAGGAGTATTTAGCCTTGGAGGA3570.21775197013687267No Hit
CAGTAGCGGCGAGCGAACGGGGAGC3490.21287237416741894No Hit
GTATCAACGCAGAGTACATAAGCAG3460.21104252567887377No Hit
CATCTAAGTACCCCGAGGAAAAGAA3400.2073828287017835No Hit
ACTCTGCGTTGATACCACTGCTTAT3360.20494303071705663No Hit
GGTTAATGAGGCGAACCGGGGGAAC3350.2043330812208749No Hit
GCCTGAATCAGTGTGTGTGTTAGTG3330.2031131822285115No Hit
CTTATACACATCTCCGAGCCCACGA3290.2006733842437846No Hit
TCACGGTACTGGTTCACTATCGGTC3180.19396393978578572No Hit
AGCGTACACGGTGGATGCCCTGGCA3150.1921340912972406No Hit
GGTATCAACGCAGAGTACATGGGGA3140.19152414180105887No Hit
CGGTACTGGTTCACTATCGGTCAGT3100.189084343816332No Hit
GGTATCAACGCAGAGTACATAAGCA3000.18298484885451483No Hit
GTGGATGCCCTGGCAGTCAGAGGCG2990.18237489935833312No Hit
TATCAACGCAGAGTACATAAGCAGT2890.17627540439651596No Hit
TTCCAGACGCTTCCACTAACACACA2840.17322565691560737No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2840.17322565691560737No Hit
GTACATGGGGGTTAAGCGACTAAGC2810.17139580842706223No Hit
GAATCAGTGTGTGTGTTAGTGGAAG2800.17078585893088052No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2740.1671261619537902No Hit
GTCCCGCCCTACTCATCGAGCTCAC2710.16529631346524506No Hit
GGTATCAACGCAGAGTACATGGGCA2660.16224656598433648No Hit
GATTCAGGCTCTGGGCTGCTCCCCG2560.15614707102251935No Hit
GGGTGACAGCCCCGTACACAAAAAT2550.1555371215263376No Hit
AGCTCACAGCATGTGCATTTTTGTG2490.15187742454924733No Hit
CTACTGGGGGAATCTCGGTTGATTT2480.15126747505306562No Hit
GGGTTTCCCCATTCGGAAATCGCCG2460.15004757606070218No Hit
GGAGTATTTAGCCTTGGAGGATGGT2430.14821772757215704No Hit
ACATAAGCAGTGGTATCAACGCAGA2420.14760777807597533No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2410.1469978285797936No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC2350.14333813160270328No Hit
CAACGCAGAGTACTTTTTTTTTTTT2350.14333813160270328No Hit
TCATTAACCTATGGATTCAGTTAAT2330.14211823261033987No Hit
CCCATGTACTCTGCGTTGATACCAC2310.14089833361797643No Hit
GGTTCACTATCGGTCAGTCAGGAGT2310.14089833361797643No Hit
GTATCAACGCAGAGTACATGGGGGG2300.14028838412179473No Hit
ACGCAGAGTACATGGGGGTTAAGCG2270.13845853563324956No Hit
CACTGATTCAGGCTCTGGGCTGCTC2260.13784858613706785No Hit
CCTATGGATTCAGTTAATGATAGTG2260.13784858613706785No Hit
GTGCTAATCTGCGATAAGCGTCGGT2250.13723863664088615No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2250.13723863664088615No Hit
GTACTTAGATGTTTCAGTTCCCCCG2230.1360187376485227No Hit
GTGGTATCAACGCAGAGTACATAAG2180.13296899016761413No Hit
GCCCTACTCATCGAGCTCACAGCAT2150.13113914167906898No Hit
TCCCCCAGTAGCGGCGAGCGAACGG2130.12991924268670554No Hit
GTTATAACCGGCGATTTCCGAATGG2100.1280893941981604No Hit
GTATCGCGCGCCTTTCCAGACGCTT2060.12564959621343352No Hit
AAGCAGTGGTATCAACGCAGAGTAC2040.1244296972210701No Hit
GTCCCCCCATATTCAGACAGGATAC2010.12259984873252494No Hit
GTACATGGGCATCTAAGTACCCCGA2000.12198989923634324No Hit
TATCAACGCAGAGTACATGGGGGTT1980.12077000024397981No Hit
GATACCACTGCTTATGTACTCTGCG1980.12077000024397981No Hit
AAGTACCCCGAGGAAAAGAAATCAA1970.12016005074779808No Hit
GGTATCAACGCAGAGTACATGGGTA1960.11955010125161637No Hit
AACGCAGAGTACTTTTTTTTTTTTT1950.11894015175543465No Hit
CTATCATTAACTGAATCCATAGGTT1910.11650035377070778No Hit
CTCCTATACTGGATGCTATTTCCTG1900.11589040427452607No Hit
TAAGTACCCCGAGGAAAAGAAATCA1870.11406055578598091No Hit
GTACGGGGCTGTCACCCTGTATCGC1830.11162075780125405No Hit
GGGCTGTCACCCTGTATCGCGCGCC1790.10918095981652719No Hit
TTTGTGTACGGGGCTGTCACCCTGT1750.10674116183180032No Hit
ATCCATAGGTTAATGAGGCGAACCG1720.10491131334325518No Hit
CTACTCATCGAGCTCACAGCATGTG1710.10430136384707346No Hit
CTGTCACCCTGTATCGCGCGCCTTT1710.10430136384707346No Hit
CCACTGCTTATGTACTCTGCGTTGA1690.10308146485471002No Hit
CTAACACACACACTGATTCAGGCTC1680.10247151535852832No Hit
CCACGTGTCCCGCCCTACTCATCGA1670.1018615658623466No Hit
GATATGAACCGTTATAACCGGCGAT1650.10064166686998316No Hit
ATACCACTGCTTATGTACTCTGCGT1640.10003171737380145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAT250.006001780719.0042741
CTCCTAT401.2713181E-519.0042721
TACACAT451.6463564E-619.0042725
ACTGCGT250.006012374518.9984768
GAAGTTT250.006012374518.9984769
TTGAAGC250.006012374518.99847617
TACTGCG307.6866726E-418.9984747
TATACAC504.5026154E-617.1038463
CTTATAC504.5026154E-617.1038461
CACATCT504.515736E-617.0986297
ACATCTC504.515736E-617.0986298
ACACATC504.515736E-617.0986296
GAAATCG453.5102436E-516.88753316
ATCGCCG453.5102436E-516.88753319
AATCGCC453.5102436E-516.88753318
ATACTGC350.00216023916.2844096
ATCTCCG551.116925E-515.54420710
CATCTCC551.116925E-515.5442079
CGAGCCC551.116925E-515.54420715
CCGAGCC551.116925E-515.54420714
Produced by FastQC (version 0.11.5)