##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295169_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163948
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.723272013077317	31.0	30.0	33.0	27.0	34.0
2	30.68075243369849	31.0	30.0	33.0	27.0	34.0
3	30.845450996657476	31.0	30.0	34.0	27.0	34.0
4	34.43872447361358	35.0	35.0	37.0	31.0	37.0
5	34.86901944518994	37.0	35.0	37.0	32.0	37.0
6	34.561775684973284	37.0	35.0	37.0	31.0	37.0
7	34.48630663381072	37.0	35.0	37.0	30.0	37.0
8	34.48317149340035	37.0	35.0	37.0	30.0	37.0
9	35.910435015980674	39.0	37.0	39.0	30.0	39.0
10	35.98849025300705	39.0	37.0	39.0	30.0	39.0
11	36.039689413716545	39.0	37.0	39.0	30.0	39.0
12	36.008209920218604	39.0	37.0	39.0	30.0	39.0
13	35.793129528875006	39.0	35.0	39.0	30.0	39.0
14	36.73883182472491	40.0	36.0	41.0	29.0	41.0
15	36.71230512113597	40.0	36.0	41.0	29.0	41.0
16	36.66783370336936	39.0	36.0	41.0	29.0	41.0
17	36.845713274940834	40.0	37.0	41.0	30.0	41.0
18	36.90445141142313	40.0	37.0	41.0	30.0	41.0
19	37.06081806426428	40.0	37.0	41.0	30.0	41.0
20	37.0731939395418	40.0	37.0	41.0	30.0	41.0
21	37.0449044819089	40.0	37.0	41.0	30.0	41.0
22	37.01473637982775	40.0	37.0	41.0	30.0	41.0
23	36.956541098397054	40.0	36.0	41.0	30.0	41.0
24	36.94217068826701	40.0	36.0	41.0	30.0	41.0
25	35.66257593871227	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1288860304073296
1101	2	0.3617861972553804
1101	3	0.42366629580777015
1101	4	-0.05529007231481131
1101	5	0.23188618747006728
1101	6	0.34212047862985173
1101	7	0.2828392595378091
1101	8	-0.12954023598776132
1101	9	0.15512663553261063
1101	10	0.45049267422675854
1101	11	0.13654068838231836
1101	12	0.1432050254556998
1101	13	0.24338364242421306
1101	14	0.25092703926267745
1101	15	0.2778226063406066
1101	16	0.5319971950811464
1101	17	0.20890017020569474
1101	18	0.5220146089727749
1101	19	-0.15422742576183168
1101	20	0.3195279451949986
1101	21	-0.23957272292991405
1101	22	-0.17673713277667957
1101	23	-0.21308729089480494
1101	24	0.0576361033314825
1101	25	-0.10817517598915316
1102	1	-0.47493570481521274
1102	2	-0.04735055143360967
1102	3	-0.5331567319107826
1102	4	-0.6180025715944737
1102	5	-0.7242165037072539
1102	6	-0.39730498094485966
1102	7	-0.7818469618648294
1102	8	-0.1961281458592552
1102	9	-0.4358662763558243
1102	10	-0.29524293344915264
1102	11	-0.3378153307498195
1102	12	-0.5871441936994941
1102	13	-0.6066855098837465
1102	14	-0.500441600165658
1102	15	-0.7672416476788726
1102	16	-0.8491760890772113
1102	17	-0.725592424734657
1102	18	-0.5097954526880315
1102	19	-0.34409373129977894
1102	20	-0.3032750283542427
1102	21	-0.22314904978982497
1102	22	-0.8267400141228407
1102	23	-0.7148449120554119
1102	24	-1.0790549587327192
1102	25	-0.4333927176246064
1103	1	-0.07998655889330308
1103	2	0.1323739201196581
1103	3	-0.032878799726233865
1103	4	0.13529881205710126
1103	5	-0.023166370750509202
1103	6	-0.23705078567120808
1103	7	0.19192224925846801
1103	8	0.17927560697640388
1103	9	0.22095508085464388
1103	10	0.3200989231378415
1103	11	0.305394861098506
1103	12	0.042139342198829866
1103	13	0.28927771292772775
1103	14	0.38813012887299436
1103	15	0.7026440245414278
1103	16	0.7170543993322624
1103	17	0.24375991815853837
1103	18	0.446516612573447
1103	19	0.2526341806032306
1103	20	0.34876889995427973
1103	21	0.3163248320965124
1103	22	0.32959308384450026
1103	23	0.185309828566254
1103	24	-0.002646143024293224
1103	25	0.64991182093285
1104	1	0.34413795472537245
1104	2	0.1323739201196581
1104	3	0.2550589434644266
1104	4	0.4232365552477617
1104	5	0.4554328510393759
1104	6	0.3971904594649729
1104	7	0.351455323188425
1104	8	0.2298592645639559
1104	9	0.5594764816328492
1104	10	0.37846468189270865
1104	11	0.6322431101257422
1104	12	0.33396813597315145
1104	13	0.3632076740172181
1104	14	0.6254842144761099
1104	15	0.6870798222067975
1104	16	0.4096614032233177
1104	17	0.6095186730223503
1104	18	0.8900963791104104
1104	19	0.47053301328805475
1104	20	0.3954615069581706
1104	21	0.3669084896840573
1104	22	0.5669471694476087
1104	23	0.3176055484106115
1104	24	0.7327624172869918
1104	25	1.1868768014775952
1105	1	-0.48905885051730635
1105	2	-0.720892514449254
1105	3	-0.9316529725122855
1105	4	-0.9623431527771587
1105	5	-0.7151736262433275
1105	6	-0.591472833007451
1105	7	-0.584122922035526
1105	8	-0.3039314378730751
1105	9	-1.1381625575994718
1105	10	-0.44937827179501255
1105	11	-1.0767911486730029
1105	12	-0.833537203250394
1105	13	-0.9710423479247936
1105	14	-1.0954426162193087
1105	15	-0.8731144377621902
1105	16	-1.1887412181160286
1105	17	-1.1144361556836557
1105	18	-1.0349935831493227
1105	19	-0.6476905612124
1105	20	-1.2450551543448967
1105	21	-1.192159413537027
1105	22	-1.114791223226682
1105	23	-1.1366087811877037
1105	24	-1.2396930404234325
1105	25	-1.4538153959420228
1106	1	-0.633159020296592
1106	2	-0.8475903073185265
1106	3	-0.7341935982702275
1106	4	-0.44545617727388986
1106	5	-0.3857714918751469
1106	6	-0.2198077832863703
1106	7	-0.6208680105632993
1106	8	-0.7298652239511725
1106	9	-0.7809590649969991
1106	10	-0.7457037629422416
1106	11	-0.5669378864852774
1106	12	-0.48636887921450267
1106	13	-0.6936048024481991
1106	14	-1.1415862009876818
1106	15	-0.5346473021813054
1106	16	-0.6057615916304542
1106	17	-0.45672332522040193
1106	18	-0.5410571291110031
1106	19	-0.7897594430945247
1106	20	-1.1110811063216133
1106	21	-0.4407041625062291
1106	22	-0.6252205222807703
1106	23	-0.8645677916169987
1106	24	-0.3371039062977914
1106	25	-0.27915495451587446
1107	1	0.18387348030976014
1107	2	0.18512252314473088
1107	3	0.395414696660648
1107	4	0.20307038105742237
1107	5	0.46151810307998176
1107	6	0.47243694142864
1107	7	0.40459888247575293
1107	8	0.5306550282923439
1107	9	0.4370254123253403
1107	10	0.1671630815884413
1107	11	0.3893742648608338
1107	12	0.47097407494509014
1107	13	0.8184313362857267
1107	14	0.6038055040814427
1107	15	0.5329005998317982
1107	16	0.33005577436517797
1107	17	0.10360895709830231
1107	18	0.38605904842962246
1107	19	0.44629440119361163
1107	20	0.19855386821149779
1107	21	0.1988180300719904
1107	22	0.437840355720688
1107	23	0.06639873760929049
1107	24	0.4670738768698044
1107	25	0.45971843864444395
1108	1	0.4200844456793895
1108	2	0.5770329561615775
1108	3	0.5022968655866116
1108	4	0.5791199941412302
1108	5	0.519308564635331
1108	6	0.42973895558812814
1108	7	0.3369845912732927
1108	8	0.4148545782621582
1108	9	0.35966961407058307
1108	10	0.5347883491368748
1108	11	0.8016458196602372
1108	12	0.5608703484756319
1108	13	0.5357629865793498
1108	14	0.7815948680021947
1108	15	0.34515020193925494
1108	16	0.9194032305969984
1108	17	0.6834344331973057
1108	18	0.40702386924077416
1108	19	0.7683403920832461
1108	20	0.6682042715922094
1108	21	0.549347638765262
1108	22	0.5771576270010783
1108	23	0.9802344217100512
1108	24	0.8432881351432258
1108	25	1.0663536397859872
1109	1	0.30246783854577686
1109	2	0.03890591780158559
1109	3	0.3300651525877001
1109	4	0.0012093454897836864
1109	5	-0.07156993616707297
1109	6	-0.050252761552215475
1109	7	0.12072430240856136
1109	8	0.08931230979396787
1109	9	0.10939783273807535
1109	10	0.11205189937759741
1109	11	-9.632811318098788E-4
1109	12	0.04637534407536492
1109	13	0.2620403588421212
1109	14	-0.018788214256957758
1109	15	-0.030529251392010792
1109	16	-0.12397824685809411
1109	17	0.17007832199991668
1109	18	0.2707708278782448
1109	19	-0.2222119711201458
1109	20	-0.08028151812668938
1109	21	0.2062301774617481
1109	22	0.6553788828896714
1109	23	0.3457673719611236
1109	24	0.5066675418834024
1109	25	0.7945981912173679
1110	1	0.3027728793449853
1110	2	-3.680850963014848E-4
1110	3	0.23489242323514858
1110	4	0.44656891233184837
1110	5	0.4238163217449227
1110	6	0.34157214502904054
1110	7	0.18020725825390826
1110	8	0.07345018823520633
1110	9	0.1518366339277364
1110	10	0.21935000049862197
1110	11	-0.06917802985444865
1110	12	0.2649752068070086
1110	13	0.018312760275811968
1110	14	-0.09051093217047423
1110	15	-0.27120644196624966
1110	16	-0.33022395722185394
1110	17	-0.32500289511287406
1110	18	-0.04643347319701974
1110	19	-0.5827701957827003
1110	20	0.17431315891136734
1110	21	-0.031205954620922682
1110	22	0.10596227341815734
1110	23	0.1618659001392686
1110	24	-0.16282075217593217
1110	25	-0.3978825530821837
1111	1	0.061743831864323084
1111	2	0.17658767250461693
1111	3	0.13093593218188104
1111	4	0.35110939751749726
1111	5	0.5074437279529747
1111	6	0.266787683147804
1111	7	0.28379010377223324
1111	8	0.32510737537626966
1111	9	-0.08065392114346537
1111	10	0.1024404642342489
1111	11	0.30697231403782865
1111	12	0.1464465398912438
1111	13	-0.16653106972930942
1111	14	0.48797538825132847
1111	15	0.05901317857595245
1111	16	0.11993588003475253
1111	17	0.525372826230587
1111	18	0.25854431565173286
1111	19	-0.10857249577854589
1111	20	0.0017442766650646035
1111	21	0.5027196760789039
1111	22	0.5284723410895111
1111	23	0.1847689875584848
1111	24	0.35337747369971595
1111	25	-0.1669834512153372
1112	1	0.02317653499444461
1112	2	-0.3172218987957294
1112	3	0.029837469459803145
1112	4	0.23590616054303837
1112	5	0.360583108767635
1112	6	0.2295881219630047
1112	7	0.1939754604520587
1112	8	0.3984379542418992
1112	9	0.36185131471970067
1112	10	0.1997491593289169
1112	11	0.5078299982903474
1112	12	0.40785502270193774
1112	13	4.773891592435575E-5
1112	14	-0.0878128406734291
1112	15	0.46684061592368664
1112	16	0.4234164824083493
1112	17	0.3639230264044073
1112	18	0.251705637226344
1112	19	0.44131423861003327
1112	20	0.6729178223773573
1112	21	0.5891434746798225
1112	22	0.33906944319953425
1112	23	0.1484812501637407
1112	24	0.22787347734701058
1112	25	-0.029169833696926162
1113	1	0.22390599402648448
1113	2	0.04531354123048459
1113	3	0.36645716770311765
1113	4	0.258445304853403
1113	5	-0.03695781644857021
1113	6	0.3864788028389228
1113	7	-0.1293373093551793
1113	8	-0.21157216130326617
1113	9	-0.3161751566647055
1113	10	-0.6272892654954845
1113	11	-0.4961164890509764
1113	12	-0.3245959311512294
1113	13	-0.4020523052505425
1113	14	-0.0564261561502164
1113	15	-0.30778218821941294
1113	16	-0.30863554853667097
1113	17	-0.2892989884412245
1113	18	-0.5252394681320496
1113	19	-0.09312848033452781
1113	20	-0.5967113217905364
1113	21	-0.8061606328014079
1113	22	-0.6336898210726503
1113	23	-0.22835842556892771
1113	24	-0.5423754220531833
1113	25	-0.8349371191690054
1114	1	-0.16864904738351782
1114	2	-0.19538026053201918
1114	3	0.2574648602375298
1114	4	0.4071971100937546
1114	5	0.0954215640224163
1114	6	0.23522176608570078
1114	7	0.13842772940222403
1114	8	-0.16070122493614747
1114	9	-0.005349609197935479
1114	10	-2.592911372616413E-4
1114	11	-0.028492922190764602
1114	12	0.11862373344248311
1114	13	0.08646783975814998
1114	14	0.06618282142058973
1114	15	-0.02922991730073221
1114	16	0.12303057778593995
1114	17	0.15156281119919868
1114	18	0.023951489455853903
1114	19	-0.08469911312653977
1114	20	-0.5374847014952095
1114	21	0.0994952957552826
1114	22	-0.22088661437631174
1114	23	7.671012665255716E-4
1114	24	-0.6227786169601899
1114	25	-0.748712945158573
1115	1	-0.1912855711650785
1115	2	-0.02271437679743471
1115	3	-0.31117870993275787
1115	4	0.36738501379311117
1115	5	0.2254567959660463
1115	6	-0.0438152274551058
1115	7	-0.23426751428538495
1115	8	0.006797307066200631
1115	9	0.09720470671516068
1115	10	-0.13590227410848854
1115	11	-0.26724806018254554
1115	12	-0.2039247104800097
1115	13	0.01973266653443062
1115	14	-0.3242164739405311
1115	15	-0.6552428356800633
1115	16	-0.4097938496949709
1115	17	-0.1681377172799543
1115	18	0.10025800736542578
1115	19	-0.11593810314415265
1115	20	-0.26784289292210417
1115	21	-0.11861979526057098
1115	22	0.11907228168945494
1115	23	-0.05780600501650213
1115	24	-0.13989546957322574
1115	25	-0.21879515302703822
1116	1	-0.1379009557804629
1116	2	-0.34994514602820104
1116	3	0.13312898237493442
1116	4	0.3227696291777278
1116	5	0.09084141135065948
1116	6	-0.06996907360895221
1116	7	-0.17919059120846015
1116	8	0.14048961475850774
1116	9	-0.05341067790022436
1116	10	-0.12667150487772005
1116	11	-0.40232498325946864
1116	12	-0.2940785566338562
1116	13	-0.38995964115787984
1116	14	0.018091218367160877
1116	15	0.1884494720122447
1116	16	0.11820615030502779
1116	17	-0.1633684865107199
1116	18	0.288104161211578
1116	19	-0.03163041083646334
1116	20	0.11308018400097097
1116	21	-0.2335428721836479
1116	22	-0.2915431029259281
1116	23	-0.35272908193958585
1116	24	-0.5495877772655362
1116	25	-2.274025922257813
1201	1	-0.33619507342752186
1201	2	0.03084897161885536
1201	3	-0.03210631174271583
1201	4	-0.38414213552815824
1201	5	-0.3716585886493391
1201	6	-0.19217495596189593
1201	7	-0.12074941473787248
1201	8	-0.13403979700619573
1201	9	0.06700108680566075
1201	10	-0.245347975465954
1201	11	-0.03444263031829564
1201	12	-0.008902086045623037
1201	13	-0.35928317056964687
1201	14	0.22250298307304206
1201	15	0.021567119071072227
1201	16	-0.01788208498908972
1201	17	-0.15133907474601926
1201	18	0.08204533768216749
1201	19	-0.18171864613057664
1201	20	0.045109595765673305
1201	21	-0.056572283948348456
1201	22	0.029309838250540565
1201	23	0.027035623942772702
1201	24	-0.1071466007949482
1201	25	-0.04243768696369443
1202	1	-0.17590095578046316
1202	2	-0.1727143767974333
1202	3	-0.1727171714712199
1202	4	-0.38261498620688883
1202	5	-0.12069705018780041
1202	6	-0.2168921505320327
1202	7	0.09650171648384998
1202	8	0.30295115322004307
1202	9	0.17028162979208616
1202	10	0.24102080281458882
1202	11	0.036598093663606335
1202	12	0.1999214433661436
1202	13	0.5466557434575066
1202	14	0.3719373722133099
1202	15	0.6293725489353221
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2211	3	-0.14096839590222032
2211	4	-0.15505808617808015
2211	5	0.20207736640684004
2211	6	0.19916950317007576
2211	7	0.31914648744322704
2211	8	-0.17708341894935842
2211	9	-0.23066910486651437
2211	10	-0.2786565236043117
2211	11	-0.05774071359655153
2211	12	0.04243080666202559
2211	13	0.23207781202564348
2211	14	-0.5920211411834018
2211	15	0.011625501974791064
2211	16	-0.18282755756014524
2211	17	0.044092187646583625
2211	18	0.3812577192640134
2211	19	0.2422871921597931
2211	20	0.39971688812082107
2211	21	0.224120049164668
2211	22	0.07322094201789042
2211	23	0.05909114277952199
2211	24	0.07244967591798712
2211	25	-0.020048394167922368
2212	1	-0.07307076710121763
2212	2	-0.09854891961310841
2212	3	-0.083021961021295
2212	4	-0.37572093686001296
2212	5	-0.061988777328586764
2212	6	-0.35034643209952065
2212	7	-0.30930379875562863
2212	8	-0.25437830976979114
2212	9	-0.19967482884361942
2212	10	-0.4251620709154551
2212	11	-0.4129664926934353
2212	12	-0.16517289625649312
2212	13	-0.19833699964844698
2212	14	-0.5083238759724651
2212	15	-0.5720788298745489
2212	16	-0.19469951007233277
2212	17	-0.19491565632204555
2212	18	0.009764538570074421
2212	19	0.18769034388051864
2212	20	-0.20790094807450288
2212	21	0.12049486366541373
2212	22	0.17502293480991682
2212	23	0.3416860124000394
2212	24	0.2118461849986133
2212	25	0.7166910588742681
2213	1	-0.02440841846703279
2213	2	-0.07104962364014256
2213	3	-0.21812474896835
2213	4	-0.24185723649391377
2213	5	-0.12259142447023663
2213	6	0.03615032937612028
2213	7	0.03657060282139213
2213	8	-0.27879396733104755
2213	9	-0.4083062002882798
2213	10	-0.3032386690568245
2213	11	-0.5985339384833495
2213	12	-0.3829143775293744
2213	13	-0.17452680533698128
2213	14	0.03824047209850079
2213	15	-0.2856102294802909
2213	16	-0.13591325268004795
2213	17	-0.3134878894957964
2213	18	0.10783550449516355
2213	19	0.32338451453667716
2213	20	0.46131898997111875
2213	21	0.17460638154769725
2213	22	0.10684495677556072
2213	23	0.38765897776190883
2213	24	0.06509878989864148
2213	25	0.08212333147353235
2214	1	0.3200760557137876
2214	2	0.2699322486078124
2214	3	0.6064163386967714
2214	4	0.43334434182140313
2214	5	0.4935234036878242
2214	6	0.5943221141305131
2214	7	0.5773151559179794
2214	8	0.3269417220381996
2214	9	0.7871103948967146
2214	10	0.8268150851605895
2214	11	0.2625485799589242
2214	12	0.05758427861518811
2214	13	0.37698288757775344
2214	14	0.19931727200700777
2214	15	0.42141498925362697
2214	16	0.5894398667801255
2214	17	0.34673113034751424
2214	18	0.3684719773035354
2214	19	0.8077872136846125
2214	20	0.6677008736561447
2214	21	0.22648777915735252
2214	22	0.3220047897943772
2214	23	0.6171866268726802
2214	24	0.40126279744711013
2214	25	0.07461775590311248
2215	1	0.22409904421953541
2215	2	0.12728562320256742
2215	3	-0.07656332531737675
2215	4	0.06738501379310691
2215	5	0.37545679596604487
2215	6	0.5831078494679716
2215	7	0.4888094087915391
2215	8	0.41448961475850865
2215	9	0.32412778363823946
2215	10	0.09871311050689258
2215	11	0.0481365552020705
2215	12	0.142229135673837
2215	13	-0.13026733346556796
2215	14	0.12193737221330991
2215	15	0.11783408739686507
2215	16	0.08635999645887438
2215	17	0.8087853596431245
2215	18	0.36179646890388995
2215	19	0.3956003583943115
2215	20	0.3821571070778944
2215	21	0.4544571278163545
2215	22	0.31522612784330306
2215	23	0.1383478411373389
2215	24	0.4639506842729233
2215	25	0.20428177004988157
2216	1	0.32589187290479416
2216	2	-0.012463074315054001
2216	3	0.12535208994465208
2216	4	0.20428357340083636
2216	5	0.09123981772516032
2216	6	-0.0318017429316626
2216	7	-0.024736407941524874
2216	8	-0.5869048075522514
2216	9	-0.47486087710340996
2216	10	-0.719261146311986
2216	11	-0.5042692063670415
2216	12	-0.40027775982110825
2216	13	-0.6542943025124615
2216	14	-1.0165701362145185
2216	15	-1.0499409662347645
2216	16	-0.773140463240793
2216	17	-1.0383645024469743
2216	18	-0.8998799025334421
2216	19	-0.916060689720922
2216	20	-0.8478919275528156
2216	21	-0.7387699638171128
2216	22	-0.49323234595382104
2216	23	-0.522179281142769
2216	24	-0.5708945501739038
2216	25	-1.701770942178129
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	39.0
10	80.0
11	145.0
12	178.0
13	231.0
14	395.0
15	542.0
16	741.0
17	987.0
18	1089.0
19	1221.0
20	1099.0
21	1064.0
22	1117.0
23	1030.0
24	1028.0
25	1158.0
26	1171.0
27	1257.0
28	1391.0
29	1781.0
30	2180.0
31	2957.0
32	3889.0
33	5590.0
34	7849.0
35	11239.0
36	17457.0
37	30856.0
38	64178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.46652024038315	18.060461852902595	13.244257344193283	21.228760562520975
2	15.7381088895124	22.073121637612385	39.02504483183487	23.163724641040343
3	19.849949678245753	30.100338528164933	29.566622952819543	20.48308884076977
4	11.969038963573816	20.424768829140945	33.26359577426989	34.34259643301535
5	11.84024303945731	39.568946585002806	31.492032893292016	17.098777482247872
6	27.333723710535306	36.44903137656761	18.795442346166986	17.4218025667301
7	23.640566768528853	36.93205731120423	19.59657694254851	19.83079897771841
8	25.278297945017474	32.96145611584514	21.30862555888327	20.451620380254113
9	27.824203977064784	16.72075149444919	18.898377455166525	36.55666707331951
10	18.92879362716992	28.21416807973357	26.22082880948605	26.636209483610457
11	29.93583174742595	27.999194847020938	19.593275752696044	22.471697652857074
12	25.536016005758167	28.36142270694945	25.235298064547614	20.867263222744768
13	30.344789722191724	23.023193392140357	27.7527664921977	18.879250393470222
14	25.575976430257597	25.645514490145725	26.70505492896748	22.0734541506292
15	28.712152633761924	24.531558786932443	26.913411569522044	19.842877009783592
16	20.98165271915485	27.6288823285432	27.208017176177812	24.181447776124138
17	19.971331849095733	28.235688798072523	26.737625423160203	25.055353929671536
18	19.542529506846808	22.660038427521425	35.18985025465857	22.607581810973194
19	21.423299561432938	26.1359130917453	32.39235587978749	20.048431467034273
20	21.688422593631817	22.516164450408684	35.24704160058558	20.548371355373916
21	24.117528653251437	24.506688300201898	31.467034274107462	19.908748772439203
22	22.469929489838243	24.145460755849417	31.66796789225852	21.716641862053823
23	24.604902803954936	21.985763691924827	31.90177313400062	21.50756037011961
24	22.164954741747383	22.573620904189134	32.00709981213555	23.25432454192793
25	22.985897623578783	25.565436978480456	32.51232128043722	18.936344117503538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	791.0
1	791.0
2	675.0
3	559.0
4	559.0
5	559.0
6	1250.5
7	1942.0
8	1942.0
9	1942.0
10	1780.0
11	1618.0
12	1618.0
13	1618.0
14	1357.5
15	1097.0
16	1097.0
17	1097.0
18	1725.0
19	2353.0
20	2353.0
21	2353.0
22	3329.5
23	4306.0
24	4306.0
25	4306.0
26	6344.5
27	8383.0
28	8383.0
29	8383.0
30	9478.5
31	10574.0
32	10574.0
33	10574.0
34	10982.0
35	11390.0
36	11390.0
37	11390.0
38	10685.5
39	9981.0
40	9981.0
41	9981.0
42	16085.5
43	22190.0
44	22190.0
45	22190.0
46	22242.5
47	22295.0
48	22295.0
49	22295.0
50	21763.5
51	21232.0
52	21232.0
53	21232.0
54	18183.0
55	15134.0
56	15134.0
57	15134.0
58	12958.0
59	10782.0
60	10782.0
61	10782.0
62	10544.5
63	10307.0
64	10307.0
65	10307.0
66	7201.0
67	4095.0
68	4095.0
69	4095.0
70	3605.0
71	3115.0
72	3115.0
73	3115.0
74	2167.5
75	1220.0
76	1220.0
77	1220.0
78	839.0
79	458.0
80	458.0
81	458.0
82	279.0
83	100.0
84	100.0
85	100.0
86	61.5
87	23.0
88	23.0
89	23.0
90	13.0
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026227828335813795
2	0.0012198989923634324
3	0.0018298484885451486
4	0.0
5	0.014638787908361189
6	0.0024397979847268648
7	6.099494961817162E-4
8	0.0030497474809085805
9	0.0048795959694537296
10	0.0012198989923634324
11	0.0024397979847268648
12	0.004269646473272014
13	0.013418888915997757
14	0.005489545465635445
15	0.0
16	0.0
17	0.0018298484885451486
18	0.0018298484885451486
19	0.0030497474809085805
20	0.0048795959694537296
21	0.0030497474809085805
22	0.0
23	6.099494961817162E-4
24	0.0
25	0.0024397979847268648
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	163948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.001219898992368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7899680402059	20.19847756605753
2	6.528580838761619	3.264449703564545
3	2.7251213740271782	2.0439407617049308
4	1.6248261728756497	1.624905457828092
5	1.1246919905340456	1.4059335886988558
6	0.8294908390055868	1.244296972210701
7	0.7197052867842592	1.259545709615244
8	0.5733245504891556	1.1467050528216263
9	0.3952279879967796	0.8893063654329422
>10	3.749786528092903	18.857808573450118
>50	0.48061675083559	8.364847390636056
>100	0.4025470248115348	20.534559738453655
>500	0.03171582619727244	5.387073950276917
>1k	0.021957110444265536	10.578354112279504
>5k	0.002439678938251726	3.199795056969283
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5246	3.199795056969283	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3777	2.3037792470783423	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3297	2.0110034889111184	No Hit
GTACATAAGCAGTGGTATCAACGCA	2374	1.4480201039353942	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1784	1.0881499011881817	No Hit
GCTTATGTACTCTGCGTTGATACCA	1455	0.8874765169443971	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1390	0.8478297996925855	No Hit
ATACAGGGTGACAGCCCCGTACACA	1103	0.6727742942884329	No Hit
CTTATGTACTCTGCGTTGATACCAC	1096	0.6685046478151608	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1067	0.6508161124258911	No Hit
GTGGTATCAACGCAGAGTACATGGG	962	0.586771415326811	No Hit
GGATACCACGTGTCCCGCCCTACTC	960	0.5855515163344476	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	890	0.5428550516017274	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	748	0.45624222314392365	No Hit
GAGTACATAAGCAGTGGTATCAACG	729	0.4446531827164711	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	722	0.4403835362431991	No Hit
GGTATCAACGCAGAGTACATGGGGG	579	0.35316075828921367	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	572	0.3488911118159416	No Hit
GTGGTATCAACGCAGAGTACTTTTT	567	0.34584136433503304	No Hit
CCCATATTCAGACAGGATACCACGT	552	0.3366921218923073	No Hit
GCGTTGATACCACTGCTTATGTACT	529	0.32266328348012785	No Hit
ATCATTAACTGAATCCATAGGTTAA	514	0.3135140410374021	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	508	0.3098543440603118	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	495	0.3019250006099495	No Hit
CATAAGCAGTGGTATCAACGCAGAG	491	0.2994852026252226	No Hit
TATGTACTCTGCGTTGATACCACTG	480	0.2927757581672238	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	452	0.2756971722741357	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	451	0.27508722277795394	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	444	0.270817576304682	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	438	0.26715787932759166	No Hit
CTGCTTATGTACTCTGCGTTGATAC	428	0.26105838436577455	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	428	0.26105838436577455	No Hit
GTTAATGATAGTGTGTCGAAACACA	425	0.2592285358772294	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	419	0.25556883890013904	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	405	0.24702954595359505	No Hit
GTATCAACGCAGAGTACATGGGGGT	402	0.24519969746504988	No Hit
CACACACACTGATTCAGGCTCTGGG	380	0.23178080854905214	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	371	0.22629126308341668	No Hit
TATTCAGACAGGATACCACGTGTCC	370	0.22568131358723498	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	357	0.21775197013687267	No Hit
CAGTAGCGGCGAGCGAACGGGGAGC	349	0.21287237416741894	No Hit
GTATCAACGCAGAGTACATAAGCAG	346	0.21104252567887377	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	340	0.2073828287017835	No Hit
ACTCTGCGTTGATACCACTGCTTAT	336	0.20494303071705663	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	335	0.2043330812208749	No Hit
GCCTGAATCAGTGTGTGTGTTAGTG	333	0.2031131822285115	No Hit
CTTATACACATCTCCGAGCCCACGA	329	0.2006733842437846	No Hit
TCACGGTACTGGTTCACTATCGGTC	318	0.19396393978578572	No Hit
AGCGTACACGGTGGATGCCCTGGCA	315	0.1921340912972406	No Hit
GGTATCAACGCAGAGTACATGGGGA	314	0.19152414180105887	No Hit
CGGTACTGGTTCACTATCGGTCAGT	310	0.189084343816332	No Hit
GGTATCAACGCAGAGTACATAAGCA	300	0.18298484885451483	No Hit
GTGGATGCCCTGGCAGTCAGAGGCG	299	0.18237489935833312	No Hit
TATCAACGCAGAGTACATAAGCAGT	289	0.17627540439651596	No Hit
TTCCAGACGCTTCCACTAACACACA	284	0.17322565691560737	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG	284	0.17322565691560737	No Hit
GTACATGGGGGTTAAGCGACTAAGC	281	0.17139580842706223	No Hit
GAATCAGTGTGTGTGTTAGTGGAAG	280	0.17078585893088052	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	274	0.1671261619537902	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	271	0.16529631346524506	No Hit
GGTATCAACGCAGAGTACATGGGCA	266	0.16224656598433648	No Hit
GATTCAGGCTCTGGGCTGCTCCCCG	256	0.15614707102251935	No Hit
GGGTGACAGCCCCGTACACAAAAAT	255	0.1555371215263376	No Hit
AGCTCACAGCATGTGCATTTTTGTG	249	0.15187742454924733	No Hit
CTACTGGGGGAATCTCGGTTGATTT	248	0.15126747505306562	No Hit
GGGTTTCCCCATTCGGAAATCGCCG	246	0.15004757606070218	No Hit
GGAGTATTTAGCCTTGGAGGATGGT	243	0.14821772757215704	No Hit
ACATAAGCAGTGGTATCAACGCAGA	242	0.14760777807597533	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA	241	0.1469978285797936	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC	235	0.14333813160270328	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	235	0.14333813160270328	No Hit
TCATTAACCTATGGATTCAGTTAAT	233	0.14211823261033987	No Hit
CCCATGTACTCTGCGTTGATACCAC	231	0.14089833361797643	No Hit
GGTTCACTATCGGTCAGTCAGGAGT	231	0.14089833361797643	No Hit
GTATCAACGCAGAGTACATGGGGGG	230	0.14028838412179473	No Hit
ACGCAGAGTACATGGGGGTTAAGCG	227	0.13845853563324956	No Hit
CACTGATTCAGGCTCTGGGCTGCTC	226	0.13784858613706785	No Hit
CCTATGGATTCAGTTAATGATAGTG	226	0.13784858613706785	No Hit
GTGCTAATCTGCGATAAGCGTCGGT	225	0.13723863664088615	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA	225	0.13723863664088615	No Hit
GTACTTAGATGTTTCAGTTCCCCCG	223	0.1360187376485227	No Hit
GTGGTATCAACGCAGAGTACATAAG	218	0.13296899016761413	No Hit
GCCCTACTCATCGAGCTCACAGCAT	215	0.13113914167906898	No Hit
TCCCCCAGTAGCGGCGAGCGAACGG	213	0.12991924268670554	No Hit
GTTATAACCGGCGATTTCCGAATGG	210	0.1280893941981604	No Hit
GTATCGCGCGCCTTTCCAGACGCTT	206	0.12564959621343352	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	204	0.1244296972210701	No Hit
GTCCCCCCATATTCAGACAGGATAC	201	0.12259984873252494	No Hit
GTACATGGGCATCTAAGTACCCCGA	200	0.12198989923634324	No Hit
TATCAACGCAGAGTACATGGGGGTT	198	0.12077000024397981	No Hit
GATACCACTGCTTATGTACTCTGCG	198	0.12077000024397981	No Hit
AAGTACCCCGAGGAAAAGAAATCAA	197	0.12016005074779808	No Hit
GGTATCAACGCAGAGTACATGGGTA	196	0.11955010125161637	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	195	0.11894015175543465	No Hit
CTATCATTAACTGAATCCATAGGTT	191	0.11650035377070778	No Hit
CTCCTATACTGGATGCTATTTCCTG	190	0.11589040427452607	No Hit
TAAGTACCCCGAGGAAAAGAAATCA	187	0.11406055578598091	No Hit
GTACGGGGCTGTCACCCTGTATCGC	183	0.11162075780125405	No Hit
GGGCTGTCACCCTGTATCGCGCGCC	179	0.10918095981652719	No Hit
TTTGTGTACGGGGCTGTCACCCTGT	175	0.10674116183180032	No Hit
ATCCATAGGTTAATGAGGCGAACCG	172	0.10491131334325518	No Hit
CTACTCATCGAGCTCACAGCATGTG	171	0.10430136384707346	No Hit
CTGTCACCCTGTATCGCGCGCCTTT	171	0.10430136384707346	No Hit
CCACTGCTTATGTACTCTGCGTTGA	169	0.10308146485471002	No Hit
CTAACACACACACTGATTCAGGCTC	168	0.10247151535852832	No Hit
CCACGTGTCCCGCCCTACTCATCGA	167	0.1018615658623466	No Hit
GATATGAACCGTTATAACCGGCGAT	165	0.10064166686998316	No Hit
ATACCACTGCTTATGTACTCTGCGT	164	0.10003171737380145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.099494961817162E-4
7	0.0	0.0	0.0	0.0	6.099494961817162E-4
8	0.0	0.0	0.0	0.0	6.099494961817162E-4
9	0.0	0.0	0.0	0.0	6.099494961817162E-4
10	0.0	0.0	0.0	0.0	6.099494961817162E-4
11	0.0	0.0	0.0	0.0	6.099494961817162E-4
12	0.0	0.0	0.0	0.0	6.099494961817162E-4
13	0.0	0.0	0.0	0.0	6.099494961817162E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCCAT	25	0.0060017807	19.004274	1
CTCCTAT	40	1.2713181E-5	19.004272	1
TACACAT	45	1.6463564E-6	19.004272	5
ACTGCGT	25	0.0060123745	18.998476	8
GAAGTTT	25	0.0060123745	18.998476	9
TTGAAGC	25	0.0060123745	18.998476	17
TACTGCG	30	7.6866726E-4	18.998474	7
TATACAC	50	4.5026154E-6	17.103846	3
CTTATAC	50	4.5026154E-6	17.103846	1
CACATCT	50	4.515736E-6	17.098629	7
ACATCTC	50	4.515736E-6	17.098629	8
ACACATC	50	4.515736E-6	17.098629	6
GAAATCG	45	3.5102436E-5	16.887533	16
ATCGCCG	45	3.5102436E-5	16.887533	19
AATCGCC	45	3.5102436E-5	16.887533	18
ATACTGC	35	0.002160239	16.284409	6
ATCTCCG	55	1.116925E-5	15.544207	10
CATCTCC	55	1.116925E-5	15.544207	9
CGAGCCC	55	1.116925E-5	15.544207	15
CCGAGCC	55	1.116925E-5	15.544207	14
>>END_MODULE