FastQCFastQC Report
Wed 25 May 2016
SRR1295168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218553
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG70590.13526738159026075No Hit
GTATCAACGCAGAGTACTTTTTTTT61750.1183278199914804No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN56580.1084208591921937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16500.011.579141
GGTATCA37350.011.4012271
TGGTATC16100.010.684912
ATAAGAC4100.09.0406343
CGTTACC850.00744870638.93985114
ATACCGT1606.7610017E-78.9052726
CGTCCTA1502.7087517E-68.86577810
GCCTACG1304.344645E-58.7740891
CCTAGTA3250.08.7731612
TAGGACC3800.08.7544274
GTATTAG6950.08.7530481
CTACGAC1752.6112866E-78.6896073
TTACACT9950.08.5973124
CTGGTCG2159.751602E-98.3945079
GCGACCT1252.7496286E-48.35884113
TAGACCG1150.00110610878.2648115
GCTAGAC1752.4272103E-68.1465063
TAGACAG5500.08.1220545
CTAGACT4600.08.0584234
GTCCTGG9400.07.98846631
Produced by FastQC (version 0.11.5)