FastQCFastQC Report
Wed 25 May 2016
SRR1295168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218553
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT175690.33666420557576016No Hit
GGTATCAACGCAGAGTACTTTTTTT148090.2837759815795681No Hit
TATCAACGCAGAGTACTTTTTTTTT101020.19357856478606233No Hit
GTGGTATCAACGCAGAGTACATGGG78330.15009907918919285No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68740.131722337590516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19900.012.1746572
GTGGTAT21300.012.091381
AGCGTCA4950.011.9003653
GGTATCA52600.011.6897351
TACCGTT856.600788E-410.0584447
TAGGACG1803.947207E-1010.0286474
TATATCG1356.442988E-79.8528965
CCGTTCA1554.0738087E-89.8059869
TCGACCG1304.246416E-69.499559
GGACCGT1554.2672946E-79.1934646
CGGTCGT1151.1123142E-49.08643910
AGGACCG2106.675691E-109.0485775
GTATTAG6200.09.0437071
TAGATCG850.00743319658.9421235
CTAGTAC4050.08.914613
ATACCGT1606.7511974E-78.9061696
GACCGTG2054.150934E-98.8045447
ACTGTCC7250.08.7788948
CAGCGTC6750.08.72685052
TAGAACG1107.1432744E-48.6371144
Produced by FastQC (version 0.11.5)