FastQCFastQC Report
Wed 25 May 2016
SRR1295167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5562439
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC83590.1502758052717522No Hit
GTGGTATCAACGCAGAGTACATGGG69850.1255744107935386No Hit
GAATGGAATGGAATGGAATGGAATG68380.12293168518342404No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN65540.11782601121558367No Hit
GTATCAACGCAGAGTACTTTTTTTT56470.10152021442392446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17000.011.5184131
TGGTATC17150.010.7516882
GGTATCA37950.010.4948581
GTCGTAA1106.800123E-59.50548651
CGATAAC1106.8547335E-59.49853815
ACCGCGT1201.7054434E-59.4985378
GTACCGT1451.711991E-69.1710856
ACTGCGT1451.712202E-69.1710028
GACGATA1054.5021865E-49.0463913
TAGGACC3500.08.9619924
GTCGTAC850.0074046358.946341
TACGCCG850.00740682948.9460165
GTCCTAA6100.08.7263481
ATTAGAC4250.08.722133
TGGACTG9300.08.6874955
GTCTAGA4850.08.6235331
AAGACCG1554.1816784E-68.585295
ACACCGC1554.220534E-68.5794026
TAAACGG1000.00289263028.5546285
CGTTATA1000.00289381258.5542422
Produced by FastQC (version 0.11.5)