##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295167_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5562439
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.49922291282655	31.0	30.0	33.0	27.0	34.0
2	30.33089405564717	31.0	30.0	34.0	26.0	34.0
3	30.559410359376525	31.0	31.0	34.0	27.0	34.0
4	33.94559976298167	35.0	35.0	37.0	28.0	37.0
5	34.22183218548554	37.0	35.0	37.0	30.0	37.0
6	34.34592055751083	37.0	35.0	37.0	30.0	37.0
7	34.15190350851488	37.0	35.0	37.0	30.0	37.0
8	34.291555916388475	37.0	35.0	37.0	30.0	37.0
9	35.28878554892916	39.0	35.0	39.0	28.0	39.0
10	35.31574674346991	39.0	35.0	39.0	28.0	39.0
11	35.7971364719685	39.0	35.0	39.0	30.0	39.0
12	35.79653655527728	39.0	35.0	39.0	30.0	39.0
13	35.74387548339856	39.0	35.0	39.0	30.0	39.0
14	36.40880646061916	40.0	36.0	41.0	27.0	41.0
15	36.78979311054018	40.0	36.0	41.0	29.0	41.0
16	36.75585871593378	40.0	36.0	41.0	29.0	41.0
17	36.39887880118775	40.0	36.0	41.0	27.0	41.0
18	36.292526713551375	39.0	36.0	41.0	27.0	41.0
19	36.7276184062423	40.0	36.0	41.0	29.0	41.0
20	36.77421523184344	40.0	36.0	41.0	29.0	41.0
21	36.82167390959253	40.0	36.0	41.0	29.0	41.0
22	36.71028895777554	40.0	36.0	41.0	28.0	41.0
23	36.415083203609065	39.0	36.0	41.0	27.0	41.0
24	36.712981481684565	40.0	36.0	41.0	29.0	41.0
25	35.44566690978544	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.16137886796972367
1101	2	0.06819564256226229
1101	3	-0.046382377413412
1101	4	0.02622008157268141
1101	5	-0.17935303227571353
1101	6	-0.28535202398637693
1101	7	-0.41956728210974603
1101	8	-0.28970249207777243
1101	9	-0.07911653589535916
1101	10	-0.09139957322957315
1101	11	-0.00838660650716605
1101	12	-0.0978890423270542
1101	13	-0.06666680751290954
1101	14	0.27333966421515754
1101	15	0.14312939633981614
1101	16	-0.019524729913413807
1101	17	0.09023217666099725
1101	18	0.30197029216813576
1101	19	0.13553585106875232
1101	20	0.08701761206618386
1101	21	-0.03477698448099886
1101	22	-0.08285950646345697
1101	23	0.022147556325855078
1101	24	-0.06679385840166674
1101	25	-0.1376929753387799
1102	1	-0.2059693081957299
1102	2	-0.02365888024680629
1102	3	-0.08051868345421553
1102	4	-0.08494487688688679
1102	5	-0.3780225134707038
1102	6	-0.34013580164010904
1102	7	-0.44460640960105735
1102	8	-0.3653008923181389
1102	9	-0.19851963546034312
1102	10	0.07932144039337174
1102	11	-0.05090460294395882
1102	12	-0.02922471349116762
1102	13	-0.1952817556873967
1102	14	0.03184515605558147
1102	15	-0.01809176186338135
1102	16	-0.10698695862906504
1102	17	-0.20513903375309894
1102	18	0.1304293072255902
1102	19	-0.04082268783118792
1102	20	-0.061350447529349594
1102	21	-0.05380264121654932
1102	22	-0.10744762456858581
1102	23	-0.05553705842054768
1102	24	-0.01138254353631396
1102	25	-0.16523080062594175
1103	1	-0.4336719144638259
1103	2	-0.2741028175599247
1103	3	-0.36878701257987956
1103	4	-0.23045430698300606
1103	5	-0.43223238944364084
1103	6	-0.46787789367822796
1103	7	-0.5443173062522746
1103	8	-0.37844823242519965
1103	9	-0.0885795999394361
1103	10	0.09437659314571079
1103	11	-0.1368931951541157
1103	12	-0.2727617501662962
1103	13	-0.16787482632709327
1103	14	-0.28105373609245277
1103	15	-0.24452133328509973
1103	16	-0.37760964937035624
1103	17	-0.18143254434748712
1103	18	0.10431880510635949
1103	19	-0.12410857013605181
1103	20	-0.17768171463318794
1103	21	-0.17518474088236502
1103	22	-0.22028572610105357
1103	23	-2.500553086652502
1103	24	-1.1538434856398396
1103	25	-0.5969144963637305
1104	1	-0.2988559301423095
1104	2	-0.13587664936861188
1104	3	-0.2720949314629806
1104	4	-0.5262357051017545
1104	5	-0.3730683790259235
1104	6	-0.5188070100006499
1104	7	-0.4677351952390296
1104	8	-0.4331182877523574
1104	9	-2.759170506738407
1104	10	-0.9005702341714397
1104	11	-0.6330799199837429
1104	12	-0.42858986131180643
1104	13	-0.2749431005659204
1104	14	-0.143070895333544
1104	15	-0.27420574445247325
1104	16	-0.25211244364582086
1104	17	0.09044599906419393
1104	18	0.1650208055040494
1104	19	-0.24770360028526994
1104	20	-0.3228105806248962
1104	21	-0.2488002132042908
1104	22	-0.25528824886731627
1104	23	-0.12772882622780912
1104	24	-0.2545924210949053
1104	25	-0.26290814845297916
1105	1	-0.20970842421464297
1105	2	-0.2422893486664428
1105	3	-0.29027167955231903
1105	4	-0.07848386460456425
1105	5	-0.07492796699394688
1105	6	-0.2311404195234772
1105	7	-0.33861733682974915
1105	8	-0.2785999519229492
1105	9	-2.23234857435628
1105	10	-0.5846647756332288
1105	11	-0.40177484090190063
1105	12	-0.21387727903532294
1105	13	-0.1963656488369736
1105	14	-0.12692702035621295
1105	15	-0.14425283997817218
1105	16	-0.15231885998607453
1105	17	-1.4970015159735297
1105	18	-0.2905347900526962
1105	19	-0.31083852126062794
1105	20	-0.20684741058628475
1105	21	-0.17672135013132362
1105	22	-0.09559627860695485
1105	23	-0.0547318720714145
1105	24	-0.0800188984357817
1105	25	-0.7724380143267879
1106	1	-0.36031008318117586
1106	2	-0.4978783917871965
1106	3	-0.5152666521205909
1106	4	-0.3462558357703074
1106	5	-0.3990153377416803
1106	6	-0.40409481341286835
1106	7	-0.46410289220197143
1106	8	-0.5235508205944015
1106	9	-3.2575181749805395
1106	10	-0.9725493956453306
1106	11	-0.7844646629737895
1106	12	-0.5835265018029716
1106	13	-0.5899647829155725
1106	14	-0.7988845217999909
1106	15	-0.8212429294922359
1106	16	-0.7822302740608222
1106	17	-0.6339822725117727
1106	18	-0.22868556315997068
1106	19	-0.4448788747793131
1106	20	-0.4967695154731189
1106	21	-0.4118328473950257
1106	22	-0.39597952359498834
1106	23	-0.2664286431711602
1106	24	-0.4430630642086868
1106	25	-0.7992123110019733
1107	1	-0.26520783754178723
1107	2	-0.10104705277701953
1107	3	-0.1458474087028705
1107	4	-1.5117421715053823
1107	5	-0.5318361204572497
1107	6	-0.319959305231869
1107	7	-0.252025068017538
1107	8	-0.1931717613251891
1107	9	0.10024617975687988
1107	10	0.33251240923963366
1107	11	-0.12602570479380404
1107	12	-0.5373923036626849
1107	13	-0.2304035537057132
1107	14	0.00207412075472746
1107	15	-0.6813800448365939
1107	16	-0.9964508420377598
1107	17	-0.14695606494459668
1107	18	-0.2882666708871895
1107	19	-0.10400007549240797
1107	20	-0.42399050718744746
1107	21	-0.17790194866657316
1107	22	-0.12700946714168992
1107	23	0.009659906127566842
1107	24	-0.24124272380068135
1107	25	-0.2985385094528894
1108	1	0.2961007501859285
1108	2	0.2852228225122708
1108	3	0.2721667153194858
1108	4	0.6707788659692611
1108	5	0.449709486137543
1108	6	0.3439500549430079
1108	7	0.34302184420217685
1108	8	0.2842699920713869
1108	9	0.5916851660595341
1108	10	0.6993569790331762
1108	11	0.4341185194058781
1108	12	0.328234555784654
1108	13	0.30399113969984626
1108	14	0.4167929250614151
1108	15	0.45257215424846464
1108	16	0.4509481738506622
1108	17	0.5219827864835196
1108	18	0.5943592290804034
1108	19	0.4383680167160975
1108	20	0.35778071645344056
1108	21	0.37811828960258964
1108	22	0.40372884642251705
1108	23	0.46354868858261256
1108	24	0.42301823313121645
1108	25	0.46427106453258915
1109	1	0.2185483280910212
1109	2	0.11639944140821612
1109	3	0.19171874095712127
1109	4	0.5891126165109384
1109	5	0.3600913841259512
1109	6	0.3526115018643168
1109	7	0.3657222547381025
1109	8	0.3050456069264129
1109	9	0.6705081175629104
1109	10	0.4408450783412974
1109	11	0.2788630598446957
1109	12	0.2754967602707836
1109	13	0.2007062934115993
1109	14	0.5600583221997795
1109	15	0.2979202496133979
1109	16	0.34409912420364464
1109	17	0.5708920940396425
1109	18	0.38177595324989966
1109	19	0.17002308392162746
1109	20	0.26671978308960576
1109	21	0.2276964693680199
1109	22	0.3531953057145216
1109	23	0.5070784869536169
1109	24	0.2393980976116481
1109	25	0.21736490618265947
1110	1	0.27981960076668955
1110	2	0.20963973105902411
1110	3	0.2846683530914511
1110	4	0.4136136805814772
1110	5	0.40790118346573934
1110	6	0.3707204558069108
1110	7	0.39529959914701607
1110	8	0.4104205742033571
1110	9	0.919427786284615
1110	10	0.8584056825204982
1110	11	0.4706506875990044
1110	12	0.3944268681184582
1110	13	0.42448900907872655
1110	14	0.6345455444838208
1110	15	0.45008730895676763
1110	16	0.4401533281020278
1110	17	0.8235791476420289
1110	18	0.9093485231831764
1110	19	0.47239570051382174
1110	20	0.5231199295168878
1110	21	0.34397836124727377
1110	22	0.4203208477615732
1110	23	0.6572769217297747
1110	24	0.3780230518531198
1110	25	0.39297256060996233
1111	1	0.2083967126204911
1111	2	0.19055024095076334
1111	3	0.2533569727564675
1111	4	0.4750818876821512
1111	5	0.43464401705421807
1111	6	0.2925089312453011
1111	7	0.24560294199022792
1111	8	0.14944546514220747
1111	9	0.5476710512475833
1111	10	0.4216666464835441
1111	11	0.26569462867802685
1111	12	0.09080577627501185
1111	13	0.1550441990626794
1111	14	0.1622913119962206
1111	15	0.222695144891496
1111	16	0.14405074814555974
1111	17	0.381580750634285
1111	18	0.5163786565129413
1111	19	0.354651758662861
1111	20	-0.18544454391471277
1111	21	-0.346800542240004
1111	22	-0.1568367071976482
1111	23	0.22434762503493744
1111	24	0.17494168176480684
1111	25	0.10005073601039527
1112	1	0.1715822529738027
1112	2	0.07386251583055525
1112	3	0.22711873695923046
1112	4	0.43929597526651065
1112	5	0.3829142500087528
1112	6	0.32427515724762657
1112	7	0.30369342954235634
1112	8	0.31383997931974505
1112	9	-1.3289951146930292
1112	10	0.08311020665086488
1112	11	0.10609159260059187
1112	12	0.17973324902928312
1112	13	0.08634402543528807
1112	14	0.5231286377937536
1112	15	0.2910774718544218
1112	16	0.2152741362715389
1112	17	0.3245219620914028
1112	18	0.3903331239933294
1112	19	0.2067476784572264
1112	20	0.23711930310508933
1112	21	0.153982865274493
1112	22	0.26734552709283577
1112	23	-0.03974167500850001
1112	24	0.13013215210924756
1112	25	0.15314599825232733
1113	1	0.25654676459230785
1113	2	0.19416362974383006
1113	3	0.294649623198314
1113	4	0.428635049045603
1113	5	0.4984242514712349
1113	6	0.39104672399754037
1113	7	0.40864117987100457
1113	8	0.3783776185845724
1113	9	0.3174195672769997
1113	10	0.5799712634529612
1113	11	0.44076885692113166
1113	12	0.21789064468664066
1113	13	0.2441754496300561
1113	14	-0.006915378815385509
1113	15	0.21874261421835683
1113	16	0.3111294566489775
1113	17	0.692446840281761
1113	18	0.4722557248412187
1113	19	0.29760973656313183
1113	20	0.34439218703715824
1113	21	0.32519561430621025
1113	22	0.3611704173002792
1113	23	0.13232917126570243
1113	24	0.22620789689761267
1113	25	0.2735067612493154
1114	1	0.31874204651059657
1114	2	0.3391612566235871
1114	3	0.3325456905612185
1114	4	0.5650457934122315
1114	5	0.4533144863736922
1114	6	0.42210977603563293
1114	7	0.2774870816742947
1114	8	0.25248431801905014
1114	9	0.06583218357047116
1114	10	0.31140384957993206
1114	11	0.3294279042198056
1114	12	0.21013413350511456
1114	13	0.3193891605966499
1114	14	0.6225813308352528
1114	15	0.2920896041459997
1114	16	0.21939609280305916
1114	17	0.5182940675346117
1114	18	0.44351036115034503
1114	19	0.35628049375026194
1114	20	0.3053127818790955
1114	21	0.2743781039380693
1114	22	0.07563501908808945
1114	23	0.5351674291348871
1114	24	0.2972784495060026
1114	25	0.20656123236191348
1115	1	0.3063480738095343
1115	2	0.2822415993483496
1115	3	0.3351001744117532
1115	4	0.5449571862976228
1115	5	0.4843688101184611
1115	6	0.3168047653263457
1115	7	0.4044293943111512
1115	8	0.3644970863075869
1115	9	0.680825486747139
1115	10	0.38906120346463524
1115	11	0.38038523630485344
1115	12	0.30089950713188784
1115	13	0.2713043775538395
1115	14	0.2782544592793599
1115	15	0.2226984219871042
1115	16	0.2800318562816173
1115	17	0.5171480366127952
1115	18	0.6100280992783667
1115	19	0.3759201486475092
1115	20	0.3814676482826016
1115	21	0.21076316222835345
1115	22	-0.23217749059327986
1115	23	0.2460025543691131
1115	24	0.31962024112988985
1115	25	0.36735355405598114
1116	1	0.6024991228627634
1116	2	0.618917948198149
1116	3	0.680929665853558
1116	4	0.8988200912066091
1116	5	0.869244105738737
1116	6	0.7011445688652245
1116	7	0.727547145594805
1116	8	0.6248083016717487
1116	9	0.9228137289461742
1116	10	0.7855778139269276
1116	11	0.4859047602535256
1116	12	0.5127452698140544
1116	13	0.45297369695001066
1116	14	0.9932430820618592
1116	15	0.6141355780179154
1116	16	0.7004641787906749
1116	17	1.0420280100290142
1116	18	0.9954014967268137
1116	19	0.6797499485589
1116	20	0.661190039415672
1116	21	0.7562378104180425
1116	22	0.7542964849446108
1116	23	0.967820088270301
1116	24	0.7717743082417812
1116	25	0.7643990330195365
1201	1	-0.17999255112564683
1201	2	-0.00714352200080981
1201	3	-0.16206034797602697
1201	4	-0.019069873706584417
1201	5	-0.21878910039807664
1201	6	-0.3087254367544361
1201	7	-0.4354474106674715
1201	8	-0.3373254016492879
1201	9	0.10764329205855461
1201	10	0.1728709642553028
1201	11	0.05480407881735516
1201	12	0.1256175405649742
1201	13	0.0060565401006229536
1201	14	0.4508258006875323
1201	15	0.1743621996885878
1201	16	0.12105537262213062
1201	17	0.22636980709235388
1201	18	0.352580116462093
1201	19	0.08236596986041889
1201	20	0.1012987826008569
1201	21	0.0882501141281864
1201	22	0.004471595560190167
1201	23	-0.02044299149358153
1201	24	-0.1011383367648051
1201	25	-0.02638014110524267
1202	1	-0.3315117959741336
1202	2	-0.30383703478224433
1202	3	-0.3803562204461244
1202	4	-0.2058380067326766
1202	5	-0.6291890842768737
1202	6	-0.5311268472698103
1202	7	-0.6183991809770859
1202	8	-0.437914292517263
1202	9	0.13678756057007035
1202	10	0.16625175708538364
1202	11	-0.08401263935966341
1202	12	-0.07306285306306393
1202	13	-0.06363654949922903
1202	14	-0.15824782219502254
1202	15	-0.19544409042229915
1202	16	-0.148428028968695
1202	17	0.1989702168296077
1202	18	0.21712003453112771
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2211	8	0.2601354602342312
2211	9	-1.387732660224124
2211	10	0.14409005031853894
2211	11	0.07911483003147879
2211	12	0.13333633999755534
2211	13	0.11690180148067242
2211	14	0.38621396182821144
2211	15	0.1939195186418914
2211	16	0.28697928574456455
2211	17	0.5748587706979009
2211	18	0.6321916757207973
2211	19	0.25331061639733576
2211	20	0.4100384111843596
2211	21	0.24211639490084025
2211	22	0.27476876042461384
2211	23	-1.7195151901864776
2211	24	-0.4880002517540447
2211	25	-0.17415102972823604
2212	1	0.05890296016563923
2212	2	-1.510707106138117
2212	3	-0.3279469209244965
2212	4	0.2778139257636383
2212	5	0.30944998451354166
2212	6	0.379279584501397
2212	7	0.3749430210472937
2212	8	0.2761294404413377
2212	9	0.7701661952076577
2212	10	0.7066586592760586
2212	11	0.4089773054303265
2212	12	0.3231319438994049
2212	13	0.20507121415855778
2212	14	0.571935782351197
2212	15	0.3030663593926022
2212	16	0.3418311419381652
2212	17	0.6250023782123719
2212	18	0.8115669989717489
2212	19	0.46501593263933216
2212	20	0.4050126184056353
2212	21	0.3444622053718547
2212	22	0.3729585160042035
2212	23	0.600096518564257
2212	24	0.3644729171191514
2212	25	0.29234311510739275
2213	1	0.2578104263774712
2213	2	0.27421224558529644
2213	3	0.26381477085452687
2213	4	0.5398321678209754
2213	5	0.4972073199388092
2213	6	0.43105195338409175
2213	7	0.3852611719893062
2213	8	0.3575716389343384
2213	9	0.5624717202607883
2213	10	0.669813155763066
2213	11	0.2897183181016132
2213	12	0.37236507022506515
2213	13	0.22200605215677882
2213	14	-1.1397622833161734
2213	15	-0.18868762136584394
2213	16	0.07024318029797882
2213	17	0.545652247714159
2213	18	0.31097164454595116
2213	19	0.21078751172658627
2213	20	0.29452970219410446
2213	21	0.3232764621987556
2213	22	0.25025808179569253
2213	23	0.48195985511739536
2213	24	0.3053925788743115
2213	25	0.26459109735305475
2214	1	0.39947702595242873
2214	2	0.41066741437360577
2214	3	0.40748429846357226
2214	4	0.6140504595323861
2214	5	0.4434503285396616
2214	6	0.3772776447287143
2214	7	0.3843689028935273
2214	8	0.23960150188445795
2214	9	0.48010287455161915
2214	10	0.3537640644810267
2214	11	0.2538036198677105
2214	12	0.11076072365450784
2214	13	0.04787673671044956
2214	14	0.3003261116562115
2214	15	0.15316263733219415
2214	16	0.3482387656128907
2214	17	0.3786602242347996
2214	18	0.5684201534314468
2214	19	0.26239891026870765
2214	20	0.3608509706152674
2214	21	0.3618845001298254
2214	22	0.40470542409561716
2214	23	0.5036158688865413
2214	24	0.3451061615832245
2214	25	0.45021015913550144
2215	1	0.34067984931514417
2215	2	0.3767366084858459
2215	3	0.3710230899774274
2215	4	0.6014625665179096
2215	5	0.5076512794764625
2215	6	0.5056259217705232
2215	7	0.4943634683998539
2215	8	0.4118678267004867
2215	9	0.7435619795654347
2215	10	0.7775990305372744
2215	11	0.42910465052165847
2215	12	0.3544947769701565
2215	13	0.2603789219223458
2215	14	0.48327588188912785
2215	15	0.36343119508621413
2215	16	0.43895451462844903
2215	17	0.6183780074700493
2215	18	0.8006670783154703
2215	19	0.41728764870163815
2215	20	-0.4440646491797722
2215	21	0.13018699995094352
2215	22	-0.625891142965223
2215	23	0.2652516530125766
2215	24	0.2579345543075888
2215	25	0.3807331376057448
2216	1	0.23132997557293322
2216	2	-0.07914882832697501
2216	3	0.3066680426926318
2216	4	0.49206829709763866
2216	5	0.4377863433642588
2216	6	0.43932207615051766
2216	7	0.5030115359030418
2216	8	0.3697690885266809
2216	9	0.7633371820295878
2216	10	0.48937861003410177
2216	11	0.3589852955867272
2216	12	-0.03043739653641353
2216	13	0.19171280239809363
2216	14	0.25609497385929814
2216	15	0.18579041260592533
2216	16	0.30273709890366973
2216	17	0.38531491866586975
2216	18	0.7444157150242887
2216	19	0.40939364132382394
2216	20	-0.7133537849429246
2216	21	-0.9283800690970381
2216	22	-0.9668388482151329
2216	23	-1.425986428760396
2216	24	-1.0476257571344192
2216	25	-0.7439095611524777
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11336.0
3	1560.0
4	1369.0
5	1015.0
6	1125.0
7	1488.0
8	592.0
9	951.0
10	1970.0
11	4190.0
12	7730.0
13	12637.0
14	18174.0
15	22959.0
16	26949.0
17	29206.0
18	30446.0
19	31622.0
20	32204.0
21	32689.0
22	33668.0
23	36007.0
24	38186.0
25	41601.0
26	46527.0
27	52606.0
28	61461.0
29	75496.0
30	94362.0
31	119289.0
32	156465.0
33	212344.0
34	294228.0
35	420699.0
36	623339.0
37	1018457.0
38	1967492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91329140908122	20.5512856706632	13.020566265476733	23.514856654778846
2	15.916006008843068	21.020511703613742	39.779305550246896	23.284176737296296
3	18.028318470399935	26.215187324917338	29.978282894613407	25.77821131006932
4	13.067573086113155	16.68734393922524	37.41158323063872	32.83349974402289
5	13.918012944260777	37.543192803362054	34.395809673202805	14.14298457917437
6	32.18595275540551	37.709481787274854	16.834616498915274	13.269948958404365
7	26.99877922962119	32.08325989528471	23.494913877689523	17.42304699740458
8	23.949395912122174	37.2391668350703	20.823728312018243	17.987708940789286
9	25.001547005979653	16.640678770297125	21.419474956092994	36.93829926763023
10	15.63524404894351	29.15317937382147	33.0673140206308	22.14426255660422
11	33.12505082644934	25.36536891752781	23.102697106097498	18.406883149925356
12	21.317237001166653	26.878997346122073	31.27219269560285	20.53157295710842
13	26.52976460997973	23.99003397753587	26.70003101983105	22.78017039265335
14	21.17957543884078	22.265832844239437	27.600804146121416	28.953787570798372
15	23.226754146729267	30.241681054913993	23.601104194607895	22.93046060374884
16	23.136072051405517	30.486624718436484	24.761773367743146	21.615529862414853
17	21.17839359731167	29.598648327767524	27.897600435352782	21.325357639568026
18	21.214454110741734	29.70111239580872	28.312791198959573	20.77164229448997
19	21.843743195360425	27.804765317846915	28.66581888487168	21.685672601920984
20	23.060597218730738	27.72492361914854	27.63381842222101	21.580660739899713
21	23.487051534971997	28.503743955142895	26.71359126527733	21.295613244607775
22	22.32914066686911	27.80258643136955	28.52646335191638	21.341809549844957
23	22.46238234312582	28.436404664465048	27.782211980785824	21.3190010116233
24	22.125536215949477	27.210335170138993	28.014641058161665	22.649487555749864
25	23.13467641344802	27.39146870470874	27.329499259468736	22.144355622374505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13726.0
1	13726.0
2	11558.5
3	9391.0
4	9391.0
5	9391.0
6	9608.0
7	9825.0
8	9825.0
9	9825.0
10	13496.0
11	17167.0
12	17167.0
13	17167.0
14	27522.0
15	37877.0
16	37877.0
17	37877.0
18	61206.5
19	84536.0
20	84536.0
21	84536.0
22	124346.0
23	164156.0
24	164156.0
25	164156.0
26	217297.0
27	270438.0
28	270438.0
29	270438.0
30	336030.0
31	401622.0
32	401622.0
33	401622.0
34	469676.0
35	537730.0
36	537730.0
37	537730.0
38	606594.5
39	675459.0
40	675459.0
41	675459.0
42	688880.5
43	702302.0
44	702302.0
45	702302.0
46	681023.5
47	659745.0
48	659745.0
49	659745.0
50	634583.0
51	609421.0
52	609421.0
53	609421.0
54	557355.0
55	505289.0
56	505289.0
57	505289.0
58	438366.5
59	371444.0
60	371444.0
61	371444.0
62	308280.5
63	245117.0
64	245117.0
65	245117.0
66	190896.5
67	136676.0
68	136676.0
69	136676.0
70	101718.5
71	66761.0
72	66761.0
73	66761.0
74	47244.0
75	27727.0
76	27727.0
77	27727.0
78	19301.0
79	10875.0
80	10875.0
81	10875.0
82	7330.5
83	3786.0
84	3786.0
85	3786.0
86	2415.5
87	1045.0
88	1045.0
89	1045.0
90	650.5
91	256.0
92	256.0
93	256.0
94	154.0
95	52.0
96	52.0
97	52.0
98	34.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1491252308564642
2	0.28669078438433215
3	0.34774313929555
4	0.37623783379916614
5	0.382853636687072
6	0.34368017339156437
7	0.3454959236406907
8	0.3631320721000266
9	0.3500982213018426
10	0.3795457352431191
11	0.29853810531675046
12	0.3457116563435572
13	0.31638998647895283
14	0.3267092007660668
15	0.36494782234915296
16	0.31477199120745414
17	0.3643545574162701
18	0.33346882545588363
19	0.3477970724712667
20	0.30923485183388083
21	0.35063755305900884
22	0.3035179352079187
23	0.285989653100016
24	0.32415636378214663
25	0.31948215522003925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5562439.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.13982652386458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.44412526081325	43.86308354908392
2	14.100465602231122	16.395972480388743
3	4.786660174302934	8.34886776487992
4	2.100982219654757	4.88602967121806
5	1.0950008492687477	3.1831579709984696
6	0.6192686312854043	2.1602502476762675
7	0.3846579864206238	1.5654764021059888
8	0.25768808840854285	1.1985552605871166
9	0.1963971697818066	1.0276647642802994
>10	0.9221541016770416	9.4733134239934
>50	0.056419320007338	2.2569158175961843
>100	0.0337901646140795	3.720644762909367
>500	0.0016453695323966444	0.6159321043942764
>1k	5.898407515636313E-4	0.6835544980578717
>5k	1.5522125039594504E-4	0.6205812818302178
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCATTCCATTCCATTCCATTCC	8359	0.1502758052717522	No Hit
GTGGTATCAACGCAGAGTACATGGG	6985	0.1255744107935386	No Hit
GAATGGAATGGAATGGAATGGAATG	6838	0.12293168518342404	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	6554	0.11782601121558367	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5647	0.10152021442392446	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.797772523887453E-5	0.0
6	0.0	0.0	0.0	1.797772523887453E-5	0.0
7	0.0	0.0	0.0	1.797772523887453E-5	0.0
8	0.0	0.0	0.0	1.797772523887453E-5	0.0
9	0.0	0.0	0.0	3.595545047774906E-5	0.0
10	0.0	0.0	0.0	3.595545047774906E-5	3.595545047774906E-5
11	0.0	0.0	0.0	5.393317571662359E-5	3.595545047774906E-5
12	0.0	0.0	0.0	5.393317571662359E-5	3.595545047774906E-5
13	0.0	0.0	0.0	5.393317571662359E-5	3.595545047774906E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	1700	0.0	11.518413	1
TGGTATC	1715	0.0	10.751688	2
GGTATCA	3795	0.0	10.494858	1
GTCGTAA	110	6.800123E-5	9.5054865	1
CGATAAC	110	6.8547335E-5	9.498538	15
ACCGCGT	120	1.7054434E-5	9.498537	8
GTACCGT	145	1.711991E-6	9.171085	6
ACTGCGT	145	1.712202E-6	9.171002	8
GACGATA	105	4.5021865E-4	9.04639	13
TAGGACC	350	0.0	8.961992	4
GTCGTAC	85	0.007404635	8.94634	1
TACGCCG	85	0.0074068294	8.946016	5
GTCCTAA	610	0.0	8.726348	1
ATTAGAC	425	0.0	8.72213	3
TGGACTG	930	0.0	8.687495	5
GTCTAGA	485	0.0	8.623533	1
AAGACCG	155	4.1816784E-6	8.58529	5
ACACCGC	155	4.220534E-6	8.579402	6
TAAACGG	100	0.0028926302	8.554628	5
CGTTATA	100	0.0028938125	8.554242	2
>>END_MODULE