FastQCFastQC Report
Wed 25 May 2016
SRR1295167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5562439
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT164150.29510435979612537No Hit
GGTATCAACGCAGAGTACTTTTTTT128580.23115759112144868No Hit
TATCAACGCAGAGTACTTTTTTTTT95380.17147154332838527No Hit
ATTCCATTCCATTCCATTCCATTCC88300.1587433138592621No Hit
GAATGGAATGGAATGGAATGGAATG77240.13885994974506688No Hit
GTGGTATCAACGCAGAGTACATGGG76180.13695431086974616No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63150.11352933488349265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT18000.012.1415891
GGTATCA49400.012.0603751
TGGTATC17200.012.0417512
GGTCGAC658.028082E-411.692497
AGCGTCA4200.011.31051353
GTACGAC1303.26836E-810.9624983
GAACCGT1502.1391315E-910.7662546
TACGACC2001.8189894E-1210.4508214
ACGTCCG1054.1086787E-59.9525378
GTTAGCG1151.02747435E-59.9131989
TACGCTG1252.5696881E-69.8811325
TCTACGC1304.239313E-69.5008323
ATAACGC900.00111357269.5008323
GTCCTAA6500.09.2097411
TAACGCT950.00182369349.0007074
TCGCCGA950.00182712768.99884717
TATCGTC1606.749178E-78.90638813
GGGTACG1502.6970974E-68.8686391
TATACCG1651.0497297E-68.6373535
TAGGACC3750.08.614014
Produced by FastQC (version 0.11.5)