FastQCFastQC Report
Wed 25 May 2016
SRR1295166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993832
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA197021.9824276135201924No Hit
GTACATAAGCAGTGGTATCAACGCA118731.1946687166442618No Hit
GTATCAACGCAGAGTACTTTTTTTT74240.7470075425222774No Hit
GCTTATGTACTCTGCGTTGATACCA71880.7232610743063214No Hit
GGTATCAACGCAGAGTACTTTTTTT51610.5193030612819873No Hit
CTTATGTACTCTGCGTTGATACCAC48250.4854945302626601No Hit
TATCAACGCAGAGTACTTTTTTTTT46390.46677909344838964No Hit
GTGGTATCAACGCAGAGTACATGGG39090.39332603498377994No Hit
GAGTACATAAGCAGTGGTATCAACG33770.3397958608698452No Hit
TATGTACTCTGCGTTGATACCACTG28750.2892843055969218No Hit
GTACTGGTTCACTATCGGTCAGTCA27290.27459369390399985No Hit
CCCATGTACTCTGCGTTGATACCAC26230.26392790733242644No Hit
GCGTTGATACCACTGCTTATGTACT24480.2463192974265268No Hit
CATAAGCAGTGGTATCAACGCAGAG21990.22126476104613255No Hit
ATACAGGGTGACAGCCCCGTACACA21600.21734055655281778No Hit
GGATACCACGTGTCCCGCCCTACTC20580.20707725249337916No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19740.19862511973854735No Hit
CTGCTTATGTACTCTGCGTTGATAC18840.1895692632155133No Hit
ACGCAGAGTACATAAGCAGTGGTAT18350.1846388524418614No Hit
ACTCTGCGTTGATACCACTGCTTAT18260.18373326678955798No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA17140.17246375644978226No Hit
GTATCAACGCAGAGTACATAAGCAG15960.16059052234180424No Hit
CATGTACTCTGCGTTGATACCACTG14720.14811356446562396No Hit
TATCAACGCAGAGTACATAAGCAGT14280.14368625683214065No Hit
GGTATCAACGCAGAGTACATAAGCA14190.14278067117983725No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13560.1364415716137134No Hit
GTACATGGGAAGCAGTGGTATCAAC13440.13523412407730884No Hit
GGTATCAACGCAGAGTACATGGGGG13070.13151116084006148No Hit
ACCCTGTATCGCGCGCCTTTCCAGA12450.12527268190197136No Hit
AAGCAGTGGTATCAACGCAGAGTAC11790.11863172045174636No Hit
CCCATATTCAGACAGGATACCACGT11780.11853109982371265No Hit
GTATTTAGCCTTGGAGGATGGTCCC11320.1139025509341619No Hit
GGTATCAACGCAGAGTACATGGGGA11270.11339944779399334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN11220.1128963446538248No Hit
GCCCAGAGCCTGAATCAGTGTGTGT11210.11279572402579108No Hit
ATCATTAACTGAATCCATAGGTTAA11160.11229262088562253No Hit
GTGGTATCAACGCAGAGTACATAAG10530.10595352131949867No Hit
GGTATCAACGCAGAGTACATGGGAA10320.10384048813079072No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG10270.10333738499062216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCATC307.6979585E-419.0080854
CGATGCT307.7241665E-418.9984849
AGCGAAG250.006047440718.99080818
CTGGCGA402.7604628E-416.62451414
CAGTCGA350.002169875416.28523815
ATCTCCC350.00217060816.28441411
TAAGACC508.677287E-515.206474
CACCGAT508.707263E-515.2003237
ATAGCTT508.7110166E-515.1995556
TCGACCG508.7448614E-515.19264818
CCAATAG456.7400653E-414.783323
TTACACC602.5561236E-514.2560644
TTGGCCG400.005264198414.2553445
CTAGGTC400.005264198414.2553443
CGTTGTA400.005265963714.2546242
TCTAGCC602.5585616E-514.2546242
TCCTAAG400.005265963714.2546242
GACCCGT400.005278336314.2495826
GACCCTT400.00528010614.24886211
GGACCCT400.00528187614.24814310
Produced by FastQC (version 0.11.5)