##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295166_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	993832
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.89482326992892	31.0	28.0	31.0	20.0	34.0
2	29.016202939732267	31.0	28.0	33.0	20.0	34.0
3	29.29477014223732	31.0	30.0	34.0	22.0	34.0
4	32.23185810076552	35.0	32.0	37.0	19.0	37.0
5	32.833344066200326	35.0	33.0	37.0	25.0	37.0
6	32.88224871004355	35.0	33.0	37.0	24.0	37.0
7	32.880309750541336	35.0	33.0	37.0	24.0	37.0
8	32.877556770158336	35.0	33.0	37.0	24.0	37.0
9	33.63837751249708	37.0	32.0	39.0	18.0	39.0
10	33.76470771719969	37.0	32.0	39.0	19.0	39.0
11	34.1415852981188	38.0	33.0	39.0	23.0	39.0
12	34.20874252388734	38.0	33.0	39.0	23.0	39.0
13	33.988304864403645	38.0	33.0	39.0	21.0	39.0
14	34.464177044007435	38.0	32.0	40.0	18.0	41.0
15	34.79065073372562	38.0	33.0	41.0	20.0	41.0
16	34.74647525939998	38.0	33.0	40.0	20.0	41.0
17	34.49020357565464	38.0	32.0	40.0	18.0	41.0
18	34.37187572949955	38.0	32.0	40.0	18.0	41.0
19	34.78638542530327	39.0	33.0	40.0	21.0	41.0
20	34.79737319788455	39.0	33.0	41.0	21.0	41.0
21	34.83727229551876	39.0	33.0	41.0	21.0	41.0
22	34.762282760064075	39.0	33.0	41.0	21.0	41.0
23	34.484669441112786	38.0	32.0	40.0	20.0	41.0
24	34.81055450015697	38.0	33.0	41.0	23.0	41.0
25	33.4471590771881	38.0	30.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07000654542802565
1101	2	0.2188865702722076
1101	3	0.12918108674966788
1101	4	0.16308570889398766
1101	5	0.21564477163543927
1101	6	-0.013724824118781953
1101	7	0.16405573709795362
1101	8	0.01687901548984172
1101	9	0.23279178240472476
1101	10	0.37351326224354153
1101	11	0.3058668492972103
1101	12	0.2752742042791425
1101	13	0.26690406436507175
1101	14	0.7869966681461378
1101	15	0.5688021490497803
1101	16	0.28798408589219804
1101	17	0.46372867839362186
1101	18	0.7556284446902168
1101	19	0.5491748021499063
1101	20	0.4802732906307554
1101	21	0.44237224418917975
1101	22	0.2634500484365745
1101	23	0.3944042970295456
1101	24	0.09208984963537148
1101	25	0.07658876062826891
1102	1	-0.21409759048645682
1102	2	-0.28565456792694377
1102	3	-0.2914942547758912
1102	4	-0.37119095266428914
1102	5	-0.6576189246442112
1102	6	-0.344237888117803
1102	7	-0.5160770183293195
1102	8	-0.2656930739038259
1102	9	-0.22372611419270783
1102	10	0.13905514319614554
1102	11	-0.25732219555946045
1102	12	-0.2290882970136252
1102	13	-0.02332684734128776
1102	14	-0.04429910756112321
1102	15	-0.06202588002102516
1102	16	-0.3914665586109578
1102	17	-0.38262383803626676
1102	18	-0.0925663823610492
1102	19	-0.08297514002814665
1102	20	-0.15447111756397902
1102	21	-0.27319408181377014
1102	22	-0.38452477316157285
1102	23	-0.27860272622145743
1102	24	-0.19123063437663035
1102	25	-0.28194397223433754
1103	1	-0.1978742981399222
1103	2	0.11603321551314139
1103	3	-0.1466978641077894
1103	4	0.06562236259121335
1103	5	-0.30579321984267693
1103	6	-0.3692180180911535
1103	7	-0.24685452078385595
1103	8	0.10847355380199986
1103	9	0.21865548382496058
1103	10	0.3504254737398824
1103	11	0.3895874308840419
1103	12	0.2966726081245312
1103	13	0.2170981374639851
1103	14	0.06628343486722343
1103	15	0.20519091365796527
1103	16	0.0019810078951891796
1103	17	0.045890819030447005
1103	18	0.47100601466545555
1103	19	0.3126534541090322
1103	20	0.20249975531460507
1103	21	0.23968403310511377
1103	22	0.29272287749998327
1103	23	-1.9161126612347843
1103	24	-0.6041975800028823
1103	25	0.1421946036691608
1104	1	-0.1444382808376119
1104	2	0.09686052299234049
1104	3	0.03398463662555429
1104	4	-0.1043984504683948
1104	5	0.02267411017236043
1104	6	-0.13967790755378218
1104	7	0.05356360287458983
1104	8	0.13920797078024094
1104	9	-2.142070691740411
1104	10	-0.33516788470570447
1104	11	0.057048284172921626
1104	12	0.09511884571002582
1104	13	0.2844952012860418
1104	14	0.6566586752417649
1104	15	0.25636915632799884
1104	16	0.4632952571583502
1104	17	0.9009564942442836
1104	18	0.9520177062969282
1104	19	0.5540747020544927
1104	20	0.46309695509669524
1104	21	0.2669421569498027
1104	22	0.437986393184282
1104	23	0.4736875427052638
1104	24	0.4727557978123471
1104	25	0.39898073202102324
1105	1	0.040801597878889595
1105	2	0.09398662344989717
1105	3	-0.02821613218600305
1105	4	0.22797904990650153
1105	5	0.023559230998664304
1105	6	-0.10537307486722369
1105	7	-0.0074777308049931435
1105	8	-0.15792745636370853
1105	9	-1.830448726113822
1105	10	-0.41107339622873695
1105	11	-0.14966491839173557
1105	12	0.045398463425613045
1105	13	0.15189075154225407
1105	14	-0.06961953798457188
1105	15	0.08638206738798715
1105	16	-0.04309900930690702
1105	17	-1.4338614890700967
1105	18	-0.04480847718642167
1105	19	-0.14449410877470115
1105	20	-0.1520857930937467
1105	21	-0.03315037840931723
1105	22	-0.09412666819994797
1105	23	-0.007887679763634026
1105	24	-0.17191299509497782
1105	25	-0.5460704550284206
1106	1	-0.20275951918358004
1106	2	-0.5205875331116196
1106	3	-0.5500410722020241
1106	4	-0.6483388375919077
1106	5	-0.36814518926402684
1106	6	-0.5752683478823286
1106	7	-0.5109611778906498
1106	8	-0.3877455350878023
1106	9	-3.086400506796032
1106	10	-0.6868546150118533
1106	11	-0.528699447472313
1106	12	-0.33870164369883327
1106	13	-0.3414748586579748
1106	14	-0.38399742658656777
1106	15	-0.6028495478316742
1106	16	-0.5317696006985173
1106	17	-0.5271905666011705
1106	18	-0.14016207346617904
1106	19	-0.30602558870932484
1106	20	-0.20061430665173674
1106	21	-0.248451478004597
1106	22	-0.05401493912013677
1106	23	-0.19222163402549342
1106	24	-0.18515180648936536
1106	25	-0.722329167213779
1107	1	-0.3023555333346195
1107	2	-0.07908813088040034
1107	3	-0.0073882453640834456
1107	4	-1.6150999078490784
1107	5	-0.15700864150426952
1107	6	-0.27195644194756596
1107	7	-0.20673418588216208
1107	8	0.1128662562689513
1107	9	0.17164743321991693
1107	10	0.5159412149458831
1107	11	-0.04651739012540901
1107	12	-0.5616402234653393
1107	13	-0.22227347594165536
1107	14	0.06626472770608416
1107	15	-0.5648284678310773
1107	16	-1.0306412357895098
1107	17	-0.014827405442431996
1107	18	-0.1446940790878699
1107	19	0.0730416929603237
1107	20	-0.27698305693728287
1107	21	-0.1408879274706365
1107	22	-0.1333723071462174
1107	23	0.20848790824600627
1107	24	-0.2711065696639139
1107	25	-0.3679135715777804
1108	1	0.07307706704779093
1108	2	0.10574098001826115
1108	3	0.22072321097753544
1108	4	0.34886041719872196
1108	5	0.38606258220509915
1108	6	0.33783402969041987
1108	7	0.24111476249260022
1108	8	0.20575384390438956
1108	9	0.5328321049853741
1108	10	0.4380619925112086
1108	11	0.40032335238574746
1108	12	0.25760690846582435
1108	13	0.23431870060391446
1108	14	0.15826082394572438
1108	15	0.3193696679241782
1108	16	0.3717954276904152
1108	17	0.5202956825116729
1108	18	0.6222709464060685
1108	19	0.3174529421636336
1108	20	0.4773696358606827
1108	21	0.2663435894191011
1108	22	0.32591187067404803
1108	23	0.6272363148744304
1108	24	0.4169542971302249
1108	25	0.4575337674916895
1109	1	0.2647131078136944
1109	2	0.2454317613647703
1109	3	0.24836334377555858
1109	4	0.7144309854507327
1109	5	0.28537311339719196
1109	6	0.4108273897892474
1109	7	0.26185287243701083
1109	8	0.32263347639358386
1109	9	0.5225564718785165
1109	10	0.3792710721900292
1109	11	0.3733008848131689
1109	12	0.2543108924065294
1109	13	0.32014366972065744
1109	14	0.5650331371019917
1109	15	0.3381154214757558
1109	16	0.13363645610301234
1109	17	0.6592614792998148
1109	18	0.21166293219977206
1109	19	-0.1300725365269102
1109	20	0.1922874091775455
1109	21	0.21615288528074927
1109	22	0.18727034812926746
1109	23	0.33024511660389777
1109	24	0.12174583511663428
1109	25	-0.18451109044531222
1110	1	0.20096680373240972
1110	2	0.15428788307032448
1110	3	0.39333354909841844
1110	4	0.3471271736319963
1110	5	0.4457446588059426
1110	6	0.2766199363331481
1110	7	0.24092026817781687
1110	8	0.29439275772844553
1110	9	0.7890551750033268
1110	10	0.7780425430797351
1110	11	0.3611660364072904
1110	12	0.4340573872210598
1110	13	0.3928274528480813
1110	14	0.5970800190384864
1110	15	0.49221938366325446
1110	16	0.5849002302038784
1110	17	0.5925177800580528
1110	18	0.8226558207328196
1110	19	0.4001766106256355
1110	20	0.4372765030061032
1110	21	0.4652043015675176
1110	22	0.5470620053239585
1110	23	0.7500168355207535
1110	24	0.6134040278303985
1110	25	0.42070065857487293
1111	1	0.1779977619118398
1111	2	0.21430404648351598
1111	3	0.10363673061499767
1111	4	0.5250802057183606
1111	5	0.4798805580359158
1111	6	0.10093448286262685
1111	7	0.0621872700454702
1111	8	-0.11281677543095725
1111	9	0.46356850981921127
1111	10	0.27660428858371944
1111	11	0.029192310089669604
1111	12	0.13668168187972185
1111	13	0.10336892719001867
1111	14	0.06756407167986112
1111	15	0.16723787034109705
1111	16	0.07719006515264937
1111	17	0.11235195139384757
1111	18	0.2927562183093926
1111	19	-0.05382726931672721
1111	20	-0.6248452432934002
1111	21	-0.5654653229676043
1111	22	-0.2869147909573755
1111	23	0.12373555958742344
1111	24	-0.04250944909334464
1111	25	-0.09332914791616531
1112	1	0.10401217593537027
1112	2	0.023580517981766747
1112	3	0.07581407324945744
1112	4	0.5932151847090239
1112	5	0.42684171699026763
1112	6	0.4862014517033657
1112	7	0.4921889237639334
1112	8	0.30991989746530635
1112	9	-1.264540614261243
1112	10	0.06632420557772178
1112	11	-0.1316362562075426
1112	12	0.01779746173244945
1112	13	0.04885703197530944
1112	14	0.09376075037303622
1112	15	0.10300673384531223
1112	16	0.11857036883225902
1112	17	0.22785049416558678
1112	18	0.24509356265092208
1112	19	-0.028658741179505398
1112	20	0.03905408259405618
1112	21	0.052359628265485014
1112	22	0.09011986066816036
1112	23	-0.17178001826270872
1112	24	0.062308917495144556
1112	25	-0.07855841908865102
1113	1	0.07405900177972669
1113	2	0.08986831102059156
1113	3	0.07650555363487754
1113	4	0.43981904190734866
1113	5	0.3998067546992772
1113	6	0.22865862176500684
1113	7	0.1690285154063531
1113	8	0.14298432175986875
1113	9	-0.043563582660311795
1113	10	0.3638020857513027
1113	11	0.1028160680034631
1113	12	-0.08382374421482552
1113	13	-0.12449394508372791
1113	14	-0.2355722180123152
1113	15	-0.17018598475647195
1113	16	0.05032543934542133
1113	17	0.30643641677740874
1113	18	0.2390410513011787
1113	19	0.10799781395850516
1113	20	0.16748181534786255
1113	21	0.056154195479713565
1113	22	0.1324867541239314
1113	23	0.04730333119145058
1113	24	0.04337038104630864
1113	25	0.13203405886697794
1114	1	0.24366002029750433
1114	2	0.21354100852453328
1114	3	0.28002837061265495
1114	4	0.4918492997528858
1114	5	0.4530822515323507
1114	6	0.4951929236470889
1114	7	0.0999802128916869
1114	8	0.1742508507458993
1114	9	-0.09223488475237218
1114	10	0.22780258544165832
1114	11	0.17910257131391205
1114	12	0.29377138965168825
1114	13	0.37695196742261317
1114	14	0.5107757897381973
1114	15	0.17434187430958303
1114	16	0.05377875152165501
1114	17	0.5254203974489613
1114	18	0.4066659007960354
1114	19	0.36733179051027065
1114	20	0.23457388329511275
1114	21	0.5723854936380306
1114	22	0.10422501206515022
1114	23	0.5549315828333832
1114	24	0.5332855400036536
1114	25	0.29533379599796206
1115	1	0.5065936587238333
1115	2	0.4239390958787794
1115	3	0.5954905056906945
1115	4	0.6356953440828619
1115	5	0.6586961592579712
1115	6	0.5550317237286606
1115	7	0.4940112754284769
1115	8	0.5981997100461243
1115	9	0.4368267619032551
1115	10	0.42298044020525083
1115	11	0.22015673258822233
1115	12	0.3115453224140836
1115	13	0.13649178834295128
1115	14	0.3241023593787986
1115	15	0.4012235768105654
1115	16	0.5007064231909766
1115	17	0.6756577466286586
1115	18	1.0030998271709777
1115	19	0.6850218398014292
1115	20	0.6834389553207458
1115	21	0.3851329594978452
1115	22	-0.046801149573418854
1115	23	0.4190628053131249
1115	24	0.47604597317072006
1115	25	0.5698488687290322
1116	1	0.6104203698525978
1116	2	0.543496775667947
1116	3	0.5249186173885256
1116	4	0.9078408121615169
1116	5	0.8815770355536969
1116	6	0.6128290211042682
1116	7	0.5192916371348417
1116	8	0.46507160291984917
1116	9	0.8439962435085562
1116	10	0.3667268465467046
1116	11	0.23751317780074288
1116	12	0.20237150491688283
1116	13	0.18948219516602194
1116	14	0.8277080906887093
1116	15	0.58853359179367
1116	16	0.47069675854330484
1116	17	0.988312640153346
1116	18	0.8304825233951973
1116	19	0.5475938980880812
1116	20	0.9351898276179753
1116	21	0.9344954211306131
1116	22	0.8615154053048428
1116	23	1.1245753246054022
1116	24	0.7692711259796781
1116	25	0.8264916238168212
1201	1	-0.07836000778214114
1201	2	0.05784557185499395
1201	3	0.05901827900474643
1201	4	0.17361381855926794
1201	5	-0.08493231575408089
1201	6	-0.27225950772454866
1201	7	-0.07130020349379862
1201	8	-0.281129659496969
1201	9	0.42197326144795966
1201	10	0.5004939653003291
1201	11	0.35998746803200277
1201	12	0.43246235064269456
1201	13	0.3116698910801077
1201	14	0.7053654157824596
1201	15	0.4120865046588733
1201	16	0.17574525762238835
1201	17	0.7184291859130383
1201	18	0.7008126130727419
1201	19	0.23756348638132607
1201	20	0.2298313705545567
1201	21	0.28512760302454865
1201	22	0.19257428564003476
1201	23	0.02214277745946447
1201	24	-0.23068175729154916
1201	25	0.046441080416862235
1202	1	-0.09789174685813862
1202	2	-0.11042509019305413
1202	3	0.12035127846219851
1202	4	0.2247900354673007
1202	5	-0.22258516830614639
1202	6	-0.03110513186377517
1202	7	0.189972976534861
1202	8	-0.04141512337577069
1202	9	0.8528414778551721
1202	10	0.7879444055991485
1202	11	0.7263258234150598
1202	12	0.5918630434351542
1202	13	0.3872468703380676
1202	14	0.4452747128346104
1202	15	0.12751852613822479
1202	16	0.263448887131446
1202	17	0.7425864970992677
1202	18	0.7909284958266554
1202	19	0.5204565208957419
1202	20	0.6647158355880123
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2211	7	0.0775605456251256
2211	8	0.17597221739836755
2211	9	-1.7492913889905317
2211	10	0.11453789612566823
2211	11	0.028764617445986573
2211	12	-0.25584188671489727
2211	13	-4.930463840366883E-4
2211	14	0.2708436552710225
2211	15	0.08309707756273355
2211	16	-0.018558487972235582
2211	17	0.374580321065892
2211	18	0.36908570544221675
2211	19	-0.3180139253062464
2211	20	0.2289584912823699
2211	21	0.05732250508175696
2211	22	-0.019637828121133794
2211	23	-2.1563630827159272
2211	24	-0.9375336546770043
2211	25	-0.27500388311072044
2212	1	-0.2191747165431721
2212	2	-1.4828105954870914
2212	3	-0.5586607069772818
2212	4	0.08502718295378031
2212	5	0.13397132180082139
2212	6	0.15620353028494804
2212	7	0.0816914569040108
2212	8	0.11637735757858536
2212	9	0.32511658774399166
2212	10	0.3163408272317838
2212	11	0.25521262111345067
2212	12	0.15417200953598353
2212	13	-0.18629738144127117
2212	14	0.34830021157949176
2212	15	-0.052285355857030424
2212	16	0.19592369760717787
2212	17	0.4223490416079869
2212	18	0.5705312020541129
2212	19	0.08400317997576678
2212	20	0.20351187723998976
2212	21	0.07643128264145815
2212	22	0.165802625727558
2212	23	0.402567765528417
2212	24	0.1595137501623256
2212	25	0.16259693633830352
2213	1	0.14871123127066355
2213	2	-0.03218973845681461
2213	3	-0.009260293421291976
2213	4	0.23035968406089324
2213	5	0.33229791944850007
2213	6	0.19347552529158918
2213	7	0.15379804695007238
2213	8	0.2994396943442723
2213	9	0.6967214011730576
2213	10	0.3866910247692772
2213	11	0.307139701359354
2213	12	0.22002992899368223
2213	13	0.11679487362444263
2213	14	-1.4471332376378498
2213	15	-0.4437516663866745
2213	16	-0.10487795940635891
2213	17	0.3944580725409992
2213	18	-0.08256959611592407
2213	19	0.07256657852727244
2213	20	0.061455046417862036
2213	21	0.24584139000561578
2213	22	0.2408257269790326
2213	23	0.3286402738187135
2213	24	0.16077394522406507
2213	25	-0.035147684121213274
2214	1	0.24255659415275233
2214	2	0.17726275049115614
2214	3	0.15657024096600125
2214	4	0.4108428278100291
2214	5	0.24173011392367272
2214	6	0.3713014460687347
2214	7	0.01213552373827298
2214	8	7.689823126924011E-4
2214	9	0.027876799333697022
2214	10	-0.11813466262142924
2214	11	-0.0418673915588883
2214	12	-0.21920537527120132
2214	13	-0.20718000181823015
2214	14	-0.18294520258253044
2214	15	-0.1596621948209176
2214	16	-0.08214848811120135
2214	17	-0.050089986461799185
2214	18	0.20161999484897564
2214	19	-0.11327802669449483
2214	20	0.12323108338086541
2214	21	0.23008205539372995
2214	22	0.31881772419192345
2214	23	0.4018052306760467
2214	24	0.3963055082028859
2214	25	0.19504169713413688
2215	1	0.5674112057629692
2215	2	0.5154037855896085
2215	3	0.5092888022225601
2215	4	0.6593400931509663
2215	5	0.5365792109714462
2215	6	0.6465328642555122
2215	7	0.462305780116246
2215	8	0.6040145744331014
2215	9	0.7721378184589369
2215	10	0.6372476093389565
2215	11	0.48640244562053425
2215	12	0.3970263229058517
2215	13	0.3247752810473514
2215	14	0.45462345896562084
2215	15	0.43886192204583807
2215	16	0.6792332474743787
2215	17	0.5903628371449869
2215	18	0.8427872198568949
2215	19	0.478386000719631
2215	20	-0.5402884795458007
2215	21	0.1494966593451963
2215	22	-0.5616510201730236
2215	23	0.4280869402297469
2215	24	0.22594093726666387
2215	25	0.5161846344615597
2216	1	-0.13391556333769472
2216	2	-0.6436283510475
2216	3	-0.06377083664151328
2216	4	-0.23250863042032321
2216	5	0.047607366785598515
2216	6	-0.18314386146604278
2216	7	-0.05950882027384097
2216	8	-0.3269346481591029
2216	9	-0.03959136440238353
2216	10	-0.3541545607767702
2216	11	-0.40758622811311795
2216	12	-0.6947485337110422
2216	13	-0.43685618601740117
2216	14	-0.5632286885259319
2216	15	-0.4766890055452109
2216	16	-0.10588739543884174
2216	17	-0.1222737619327674
2216	18	0.3030801867688808
2216	19	-0.2297834523941873
2216	20	-1.369148221282174
2216	21	-1.530065140739623
2216	22	-1.1761331973531668
2216	23	-1.8340930502049346
2216	24	-1.4430400339355387
2216	25	-1.208094066975434
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1999.0
3	284.0
4	208.0
5	223.0
6	221.0
7	251.0
8	109.0
9	231.0
10	592.0
11	1340.0
12	2863.0
13	4953.0
14	7612.0
15	10541.0
16	12423.0
17	13795.0
18	13281.0
19	12635.0
20	11969.0
21	11412.0
22	10910.0
23	10460.0
24	10936.0
25	11075.0
26	11493.0
27	12718.0
28	14056.0
29	16467.0
30	19890.0
31	24559.0
32	31790.0
33	41311.0
34	55874.0
35	76149.0
36	111124.0
37	172290.0
38	255788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.851033299979946	21.623775824942086	12.604959880209954	20.92023099486801
2	15.312744390681999	24.649717200563074	37.32841566724017	22.709122741514758
3	19.06998533978975	30.915677539992487	27.63358763211353	22.38074948810423
4	12.703553783444224	22.289518015343038	33.00075947628455	32.006168724928195
5	12.910514317688971	42.079996444692235	29.97659759067297	15.03289164694582
6	28.214450501132305	38.899114247261615	17.717780782002652	15.168654469603426
7	23.736842902361083	38.464614033227825	19.19732238864741	18.60122067576368
8	23.02399841653269	37.824671390138874	20.44349203015051	18.70783816317792
9	25.435862329398535	22.022368898003116	18.418849890901896	34.12291888169646
10	16.57810184769006	32.286952033590325	26.499954042585856	24.63499207613376
11	29.290661263026813	30.965944353677376	19.47419462589448	20.269199757401328
12	22.00797227876321	32.139006009498885	24.84713741074602	21.00588430099189
13	27.1942910782958	27.304310962623518	24.6074106971627	20.893987261917978
14	23.221083425784133	28.803765733881004	23.836760322102286	24.138390518232576
15	26.572637004839507	30.68449904260218	21.927237765910174	20.815626186648135
16	21.80538172715895	32.13825749929347	23.626811740482054	22.429549033065527
17	20.020399816199678	33.18848117309042	24.12217672098202	22.668942289727884
18	19.526491022268168	29.711113489110225	29.10923294049237	21.65316254812924
19	22.00605815831987	31.67316235864297	25.37166801292407	20.949111470113085
20	22.94895499852646	29.40830349248899	27.055283948922703	20.587457560061846
21	24.340021385881887	30.20326684996461	24.608809004284243	20.84790275986926
22	23.06137340052015	29.43264952279262	24.835876387292366	22.670100689394864
23	23.795301380243515	28.49523286753436	24.81259907351444	22.896866678707685
24	22.39286128874331	29.264981249779186	24.740197142294747	23.601960319182762
25	23.291245544848273	30.184504601259505	24.83125687009378	21.69299298379844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23554.0
1	23554.0
2	16235.0
3	8916.0
4	8916.0
5	8916.0
6	9300.0
7	9684.0
8	9684.0
9	9684.0
10	10019.5
11	10355.0
12	10355.0
13	10355.0
14	11460.0
15	12565.0
16	12565.0
17	12565.0
18	15942.0
19	19319.0
20	19319.0
21	19319.0
22	24069.5
23	28820.0
24	28820.0
25	28820.0
26	35993.0
27	43166.0
28	43166.0
29	43166.0
30	48512.5
31	53859.0
32	53859.0
33	53859.0
34	57976.5
35	62094.0
36	62094.0
37	62094.0
38	66853.5
39	71613.0
40	71613.0
41	71613.0
42	100377.5
43	129142.0
44	129142.0
45	129142.0
46	125712.5
47	122283.0
48	122283.0
49	122283.0
50	118768.0
51	115253.0
52	115253.0
53	115253.0
54	105607.5
55	95962.0
56	95962.0
57	95962.0
58	83509.5
59	71057.0
60	71057.0
61	71057.0
62	64273.0
63	57489.0
64	57489.0
65	57489.0
66	43824.0
67	30159.0
68	30159.0
69	30159.0
70	24502.0
71	18845.0
72	18845.0
73	18845.0
74	13001.5
75	7158.0
76	7158.0
77	7158.0
78	4591.0
79	2024.0
80	2024.0
81	2024.0
82	1204.5
83	385.0
84	385.0
85	385.0
86	245.5
87	106.0
88	106.0
89	106.0
90	61.0
91	16.0
92	16.0
93	16.0
94	10.5
95	5.0
96	5.0
97	5.0
98	4.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14378687746017435
2	0.2864669280119779
3	0.3417076528024857
4	0.3698814286519251
5	0.378635423290858
6	0.3395946196137778
7	0.34180827343051945
8	0.36223426092136296
9	0.34603433980793535
10	0.3808490771075997
11	0.29129671815759606
12	0.34211013531462053
13	0.31212518816057444
14	0.32349531912838386
15	0.36706405106698115
16	0.3091065693195631
17	0.3649510178782732
18	0.32902945367023806
19	0.3453299954116994
20	0.30387429666181004
21	0.3480467523686096
22	0.29813892086388843
23	0.2792222427935506
24	0.31967173526310283
25	0.31443946260534983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	993832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.496216472985886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.44301559394701	20.78443121971027
2	7.054442498556678	3.738320710759782
3	2.9620259871582255	2.3544744526306216
4	1.7989458984615143	1.9066103979530549
5	1.3012545735826715	1.7239161434054702
6	0.9677715641156652	1.5385370915525283
7	0.7661026053528739	1.4209174330363754
8	0.6013779435467419	1.2747392139434843
9	0.5046878732317813	1.2035087226396122
>10	4.758894755768301	26.153887464314995
>50	0.5529511698693265	10.02308555479987
>100	0.2514110151470283	11.891432054070304
>500	0.022194579723786363	4.156049520781891
>1k	0.013010615700150625	6.623824326498175
>5k	0.001147995502954467	2.0048198871534604
>10k+	7.65330335302978E-4	3.2014458067501397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	19702	1.9824276135201924	No Hit
GTACATAAGCAGTGGTATCAACGCA	11873	1.1946687166442618	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7424	0.7470075425222774	No Hit
GCTTATGTACTCTGCGTTGATACCA	7188	0.7232610743063214	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5161	0.5193030612819873	No Hit
CTTATGTACTCTGCGTTGATACCAC	4825	0.4854945302626601	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4639	0.46677909344838964	No Hit
GTGGTATCAACGCAGAGTACATGGG	3909	0.39332603498377994	No Hit
GAGTACATAAGCAGTGGTATCAACG	3377	0.3397958608698452	No Hit
TATGTACTCTGCGTTGATACCACTG	2875	0.2892843055969218	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2729	0.27459369390399985	No Hit
CCCATGTACTCTGCGTTGATACCAC	2623	0.26392790733242644	No Hit
GCGTTGATACCACTGCTTATGTACT	2448	0.2463192974265268	No Hit
CATAAGCAGTGGTATCAACGCAGAG	2199	0.22126476104613255	No Hit
ATACAGGGTGACAGCCCCGTACACA	2160	0.21734055655281778	No Hit
GGATACCACGTGTCCCGCCCTACTC	2058	0.20707725249337916	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1974	0.19862511973854735	No Hit
CTGCTTATGTACTCTGCGTTGATAC	1884	0.1895692632155133	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1835	0.1846388524418614	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1826	0.18373326678955798	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1714	0.17246375644978226	No Hit
GTATCAACGCAGAGTACATAAGCAG	1596	0.16059052234180424	No Hit
CATGTACTCTGCGTTGATACCACTG	1472	0.14811356446562396	No Hit
TATCAACGCAGAGTACATAAGCAGT	1428	0.14368625683214065	No Hit
GGTATCAACGCAGAGTACATAAGCA	1419	0.14278067117983725	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1356	0.1364415716137134	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1344	0.13523412407730884	No Hit
GGTATCAACGCAGAGTACATGGGGG	1307	0.13151116084006148	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1245	0.12527268190197136	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1179	0.11863172045174636	No Hit
CCCATATTCAGACAGGATACCACGT	1178	0.11853109982371265	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1132	0.1139025509341619	No Hit
GGTATCAACGCAGAGTACATGGGGA	1127	0.11339944779399334	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	1122	0.1128963446538248	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	1121	0.11279572402579108	No Hit
ATCATTAACTGAATCCATAGGTTAA	1116	0.11229262088562253	No Hit
GTGGTATCAACGCAGAGTACATAAG	1053	0.10595352131949867	No Hit
GGTATCAACGCAGAGTACATGGGAA	1032	0.10384048813079072	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	1027	0.10333738499062216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.0062062803371193E-4	0.0	0.0	0.0	0.0
9	1.0062062803371193E-4	0.0	0.0	0.0	0.0
10	1.0062062803371193E-4	0.0	0.0	0.0	0.0
11	1.0062062803371193E-4	0.0	0.0	0.0	0.0
12	1.0062062803371193E-4	0.0	0.0	0.0	0.0
13	1.0062062803371193E-4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCATC	30	7.6979585E-4	19.008085	4
CGATGCT	30	7.7241665E-4	18.998484	9
AGCGAAG	25	0.0060474407	18.990808	18
CTGGCGA	40	2.7604628E-4	16.624514	14
CAGTCGA	35	0.0021698754	16.285238	15
ATCTCCC	35	0.002170608	16.284414	11
TAAGACC	50	8.677287E-5	15.20647	4
CACCGAT	50	8.707263E-5	15.200323	7
ATAGCTT	50	8.7110166E-5	15.199555	6
TCGACCG	50	8.7448614E-5	15.192648	18
CCAATAG	45	6.7400653E-4	14.78332	3
TTACACC	60	2.5561236E-5	14.256064	4
TTGGCCG	40	0.0052641984	14.255344	5
CTAGGTC	40	0.0052641984	14.255344	3
CGTTGTA	40	0.0052659637	14.254624	2
TCTAGCC	60	2.5585616E-5	14.254624	2
TCCTAAG	40	0.0052659637	14.254624	2
GACCCGT	40	0.0052783363	14.249582	6
GACCCTT	40	0.005280106	14.248862	11
GGACCCT	40	0.005281876	14.248143	10
>>END_MODULE