FastQCFastQC Report
Wed 25 May 2016
SRR1295166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993832
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT227262.2867043926941375No Hit
GGTATCAACGCAGAGTACTTTTTTT165451.6647682908177641No Hit
TATCAACGCAGAGTACTTTTTTTTT143621.4451134598201707No Hit
GTACATAAGCAGTGGTATCAACGCA131851.326682980624492No Hit
GCTTATGTACTCTGCGTTGATACCA76340.7681378744093569No Hit
ACGCAGAGTACTTTTTTTTTTTTTT75620.7608931891909296No Hit
GTACTTTTTTTTTTTTTTTTTTTTT61390.6177100354989575No Hit
CTTATGTACTCTGCGTTGATACCAC57230.5758518542369334No Hit
GTGGTATCAACGCAGAGTACATGGG42210.4247196709302981No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40970.4122427130541178No Hit
GAGTACATAAGCAGTGGTATCAACG37930.3816540421318694No Hit
CCCATGTACTCTGCGTTGATACCAC32010.32208663033591195No Hit
GTACTGGTTCACTATCGGTCAGTCA30110.3029687110095066No Hit
GCAGAGTACTTTTTTTTTTTTTTTT30080.3026668491254055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28490.28666816926804534No Hit
TATGTACTCTGCGTTGATACCACTG27970.2814358966102923No Hit
GCGTTGATACCACTGCTTATGTACT27490.27660610646467415No Hit
CATAAGCAGTGGTATCAACGCAGAG27180.273486866995629No Hit
ATACAGGGTGACAGCCCCGTACACA25470.2562807396018643No Hit
ATCAACGCAGAGTACTTTTTTTTTT23740.23887337095203215No Hit
GTGGTATCAACGCAGAGTACTTTTT23260.23404358080641396No Hit
ACGCAGAGTACATAAGCAGTGGTAT21820.21955421036955947No Hit
GGATACCACGTGTCCCGCCCTACTC21760.21895048660135719No Hit
CTGCTTATGTACTCTGCGTTGATAC21740.21874924534528972No Hit
ACTCTGCGTTGATACCACTGCTTAT21220.21351697268753672No Hit
CATGTACTCTGCGTTGATACCACTG18650.18765747128287277No Hit
GTATCAACGCAGAGTACATAAGCAG17350.17457678963849022No Hit
GGTATCAACGCAGAGTACATAAGCA16030.16129486673804025No Hit
TATCAACGCAGAGTACATAAGCAGT15780.15877935103719742No Hit
GGTATCAACGCAGAGTACATGGGGG14900.1499247357702308No Hit
ACCCTGTATCGCGCGCCTTTCCAGA14280.14368625683214065No Hit
CCCATATTCAGACAGGATACCACGT13750.1383533635463539No Hit
GTACATGGGAAGCAGTGGTATCAAC13410.1349322621932077No Hit
GGTATCAACGCAGAGTACATGGGGA13340.1342279177969717No Hit
AAGCAGTGGTATCAACGCAGAGTAC12880.12959936890742096No Hit
GCCCAGAGCCTGAATCAGTGTGTGT12860.12939812765135353No Hit
GTGGTATCAACGCAGAGTACATAAG12340.12416585499360053No Hit
ATCATTAACTGAATCCATAGGTTAA11880.11953730610404978No Hit
ACATAAGCAGTGGTATCAACGCAGA11660.11732365228730812No Hit
GCCTTGGAGGATGGTCCCCCCATAT11280.11350006842202706No Hit
CTTATACACATCTCCGAGCCCACGA11250.11319820653792592No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG11120.11189013837348769No Hit
GTATTTAGCCTTGGAGGATGGTCCC11050.11118579397725169No Hit
TCTAAGTACCCCGAGGAAAAGAAAT10580.10645662445966722No Hit
GGTATCAACGCAGAGTACATGGGAA10540.10605414194753238No Hit
CAACGCAGAGTACTTTTTTTTTTTT10380.10444421189899299No Hit
GTTAATGATAGTGTGTCGAAACACA10170.10233117871028503No Hit
CGGTACTGGTTCACTATCGGTCAGT10150.10212993745421761No Hit
ATCATGTACTCTGCGTTGATACCAC10090.10152621368601535No Hit
GTATCAACGCAGAGTACATGGGGAG10030.10092248991781308No Hit
GTTCACTATCGGTCAGTCAGGAGTA10020.10082186928977936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCCC359.930808E-519.0010576
TTTAGGC307.719778E-419.00013
AACCACG359.942321E-518.99818815
GCAATTA250.006033903518.99818818
GACCTAT250.006033903518.99818817
TATCCCG504.558735E-617.100094
TACTGCG402.7586892E-416.6259257
CTTATAC1200.016.6259231
TAATACG402.7597483E-416.6250885
TACGGTC402.7597483E-416.6250884
ACCGTTC350.002168653516.286628
GGTTATC350.002168653516.286627
GTTATCG350.002168653516.286628
CAGTCGA350.002170841416.2841615
CACCGTT508.704773E-515.2008457
TAGTAGC508.70851E-515.200084
GTCCAAT456.7564426E-414.7786011
GCTATGG456.7590206E-414.7778573
AAGACCG456.7590206E-414.7778575
TATACAC1350.014.7778573
Produced by FastQC (version 0.11.5)