FastQCFastQC Report
Wed 25 May 2016
SRR1295165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036852
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA110431.0650507497694945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA91760.8849864783016285No Hit
GCTTATGTACTCTGCGTTGATACCA66060.6371208234154923No Hit
GTATCAACGCAGAGTACTTTTTTTT55830.5384567903615944No Hit
CTTATGTACTCTGCGTTGATACCAC46470.4481835401773831No Hit
GGTATCAACGCAGAGTACTTTTTTT40850.39398101175481165No Hit
GTGGTATCAACGCAGAGTACATGGG39010.37623498821432566No Hit
TATCAACGCAGAGTACTTTTTTTTT35550.34286474829580305No Hit
GAGTACATAAGCAGTGGTATCAACG31210.3010072797274828No Hit
CCCATGTACTCTGCGTTGATACCAC30680.2958956533815819No Hit
TATGTACTCTGCGTTGATACCACTG27750.2676370398089602No Hit
GCGTTGATACCACTGCTTATGTACT22460.21661722213006293No Hit
CATGTACTCTGCGTTGATACCACTG21000.20253613823380773No Hit
CATAAGCAGTGGTATCAACGCAGAG20640.19906409014979956No Hit
ACTCTGCGTTGATACCACTGCTTAT19390.18700836763588247No Hit
CTGCTTATGTACTCTGCGTTGATAC17690.17061258501695517No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17430.1681049947340604No Hit
ACGCAGAGTACATAAGCAGTGGTAT17350.1673334284931697No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16840.1624146937074915No Hit
GTATCAACGCAGAGTACATAAGCAG15880.15315589881680317No Hit
GTACTGGTTCACTATCGGTCAGTCA15240.1469833688896776No Hit
GTACATGATAAGCAGTGGTATCAAC15090.14553668218800755No Hit
GTACATGGGAAGCAGTGGTATCAAC14880.14351132080566947No Hit
ATCATGTACTCTGCGTTGATACCAC14390.13878547758021395No Hit
GGTATCAACGCAGAGTACATGGGGG13510.1302982489304163No Hit
ATTCCATTCCATTCCATTCCATTCC13390.12914089956908026No Hit
GGTATCAACGCAGAGTACATAAGCA13030.12566885148507212No Hit
TATCAACGCAGAGTACATAAGCAGT12700.12248614074139799No Hit
GAATGGAATGGAATGGAATGGAATG12460.12017144201872591No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA12020.11592782769382709No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN11640.11226288804959626No Hit
ATACAGGGTGACAGCCCCGTACACA11430.11023752666725821No Hit
GGTATCAACGCAGAGTACATGGGGA11300.10898373152581083No Hit
GGATACCACGTGTCCCGCCCTACTC11270.10869439418547681No Hit
AAGCAGTGGTATCAACGCAGAGTAC11160.10763349060425209No Hit
GGTATCAACGCAGAGTACATGGGAA10780.1039685509600213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA1900.016.0090481
GTGCGGT704.4374246E-714.92689911
GACCGAA456.7307043E-414.7861341
GTCCTTA400.00525776214.2580591
CATCTAG602.5529715E-514.2580581
CAGGAAC400.005264519214.25533
AGTACGG400.005264519214.25533
TCTAACC707.2291023E-613.5764753
GTCCGTG707.26532E-613.56990810
GAGCCAG655.4547734E-513.15237210
TCTGCAC751.471812E-512.6713773
TCCGTGA751.479152E-512.66524811
ATCGCCG751.4813602E-512.66341119
GTCCTAC701.0844799E-412.2211931
CAGTCTA701.0913597E-412.2129179
TTATCCG550.003058721512.0954054
TAGGACC550.003058721512.0954054
GCGTGAG550.00306982512.0895559
GGGGCAT550.003070937212.088977
GGTGCGG802.8674707E-511.87367110
Produced by FastQC (version 0.11.5)