##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295165_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1036852
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46412024088298	31.0	30.0	33.0	23.0	34.0
2	29.59239409288886	31.0	30.0	34.0	23.0	34.0
3	29.53973373249027	31.0	30.0	34.0	23.0	34.0
4	32.46038007353026	35.0	33.0	37.0	19.0	37.0
5	33.18385651954184	35.0	33.0	37.0	26.0	37.0
6	33.30693483737313	36.0	33.0	37.0	26.0	37.0
7	33.28697634763689	36.0	33.0	37.0	26.0	37.0
8	33.24933355965943	36.0	33.0	37.0	25.0	37.0
9	34.0130780477831	38.0	33.0	39.0	19.0	39.0
10	34.124708251515166	38.0	33.0	39.0	22.0	39.0
11	34.53203446586398	38.0	34.0	39.0	25.0	39.0
12	34.575672323533155	38.0	34.0	39.0	25.0	39.0
13	34.409293708263085	38.0	34.0	39.0	23.0	39.0
14	34.961284734947704	39.0	33.0	41.0	19.0	41.0
15	35.292898118535724	39.0	34.0	41.0	23.0	41.0
16	35.25011284156273	39.0	34.0	41.0	23.0	41.0
17	35.00754591783591	39.0	33.0	41.0	21.0	41.0
18	34.91249088587378	39.0	33.0	41.0	20.0	41.0
19	35.34064649535324	39.0	34.0	41.0	24.0	41.0
20	35.358963477911985	39.0	34.0	41.0	23.0	41.0
21	35.400490137454526	39.0	34.0	41.0	24.0	41.0
22	35.32561059823389	39.0	34.0	41.0	23.0	41.0
23	35.06231940527674	39.0	33.0	41.0	22.0	41.0
24	35.38429785543163	39.0	34.0	41.0	24.0	41.0
25	34.140993121486964	38.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.032992714789937594
1101	2	0.2433602409412856
1101	3	-0.07379334275358573
1101	4	-0.029033033899395377
1101	5	-0.20962451051719455
1101	6	-0.1803968958807829
1101	7	-0.14886255111752433
1101	8	-0.031444717975205094
1101	9	0.036157192208243316
1101	10	0.1522367831259004
1101	11	0.11618453189136346
1101	12	0.3261373537575665
1101	13	0.1120037365019968
1101	14	0.4396437063359784
1101	15	0.3232206520993941
1101	16	0.26760812454273264
1101	17	0.3150447555375351
1101	18	0.5741098591102087
1101	19	0.16687426623094126
1101	20	0.1464964837267857
1101	21	0.09135760822907457
1101	22	0.04164909701460573
1101	23	0.0690608661993295
1101	24	-0.17148825679038282
1101	25	0.05827507955017097
1102	1	-0.29207524163490817
1102	2	0.058613263633038315
1102	3	-0.03434568512940217
1102	4	-0.2281444638228507
1102	5	-0.4988361014456473
1102	6	-0.21645550198837782
1102	7	-0.38228794867245597
1102	8	-0.17169518133886896
1102	9	0.021713275140498922
1102	10	0.20567734335745058
1102	11	-0.10013453148762608
1102	12	-0.028372540282667558
1102	13	-0.08699637159829621
1102	14	0.06999368639827708
1102	15	0.10397894342752778
1102	16	-0.10834213786893088
1102	17	-0.18525950024923077
1102	18	0.12293110060267765
1102	19	-0.060915956205100485
1102	20	0.07568900318662486
1102	21	-0.10696347254475569
1102	22	-0.10579719468006488
1102	23	-0.07032228919806016
1102	24	-0.02034149883266423
1102	25	-0.4336070181109193
1103	1	-0.399686385051659
1103	2	-0.07867498878994539
1103	3	-0.5249269006508719
1103	4	-0.32002280168182295
1103	5	-0.47348138919356586
1103	6	-0.5251818377002735
1103	7	-0.5487334052244677
1103	8	-0.4226292135495058
1103	9	0.0012063642187598589
1103	10	0.051030941003872954
1103	11	-0.1653470288569352
1103	12	-0.23080142734158215
1103	13	-0.36792099565720093
1103	14	-0.5100232673404577
1103	15	-0.3994789006464714
1103	16	-0.2750655125428949
1103	17	-0.2084011336304954
1103	18	0.04911450749754209
1103	19	-0.03903632041716065
1103	20	-0.2610143012525086
1103	21	-0.19511998077853576
1103	22	-0.31574270366254353
1103	23	-2.6171023621020666
1103	24	-1.1521098867095674
1103	25	-0.7271387854721922
1104	1	-0.47319128847708214
1104	2	-0.2459495577436961
1104	3	-0.4478229058327976
1104	4	-0.9454992930527801
1104	5	-0.49699592019769057
1104	6	-0.7229761999967579
1104	7	-0.5534361638761922
1104	8	-0.6113581592152926
1104	9	-2.7286065213915904
1104	10	-1.0401403848944568
1104	11	-0.8024802679063967
1104	12	-0.6341560298775093
1104	13	-0.6648524908238613
1104	14	-0.6839434477044719
1104	15	-0.7309228677695785
1104	16	-0.3995198575318639
1104	17	-0.2532018353740071
1104	18	-0.1876623267037516
1104	19	-0.3828710919853364
1104	20	-0.6678743015033461
1104	21	-0.7070659246906956
1104	22	-0.4699691369360437
1104	23	-0.4194419006127532
1104	24	-0.45158881706712606
1104	25	-0.6636413996733452
1105	1	-0.4015261304094295
1105	2	-0.22712931942229986
1105	3	-0.25857381564837567
1105	4	-0.2245893657262812
1105	5	-0.28657652967169156
1105	6	-0.3262388712972424
1105	7	-0.2978578862120216
1105	8	-0.4874235839883454
1105	9	-2.08159783111628
1105	10	-0.6572225681538555
1105	11	-0.5961843813530407
1105	12	-0.49632260155831176
1105	13	-0.22145549833705758
1105	14	-0.43017995878057036
1105	15	-0.3085516481138413
1105	16	-0.4348072922058037
1105	17	-1.4270587456887256
1105	18	-0.5732516674273569
1105	19	-0.49502779258617124
1105	20	-0.42262489842430995
1105	21	-0.4976969660710111
1105	22	-0.3671691026666366
1105	23	-0.31142658367658527
1105	24	-0.35525787886754046
1105	25	-1.0119750496262299
1106	1	-0.48085207031819266
1106	2	-0.3946336453622088
1106	3	-0.4192628198956605
1106	4	-0.09070152555009514
1106	5	-0.12563190170252625
1106	6	-0.3783939759063344
1106	7	-0.4069974174374451
1106	8	-0.4553565712464902
1106	9	-2.8365136719216935
1106	10	-0.7344248648346792
1106	11	-0.5795609740854601
1106	12	-0.4776266758071728
1106	13	-0.6455037915571324
1106	14	-0.6475859679043694
1106	15	-0.7165389062544705
1106	16	-0.7141922222042325
1106	17	-0.6323363923774181
1106	18	-0.09669039638755805
1106	19	-0.5220845803791505
1106	20	-0.45110138104190156
1106	21	-0.3508588348770516
1106	22	-0.4894988179411328
1106	23	-0.39980946868524114
1106	24	-0.49454921125703066
1106	25	-0.7924571952924211
1107	1	-0.20810555713224943
1107	2	-0.07098269219819286
1107	3	-0.17419612886697422
1107	4	-1.3770724982037272
1107	5	-0.2553581335071442
1107	6	-0.23608323834618972
1107	7	-0.1451270233817752
1107	8	-0.24138799825178836
1107	9	-0.0018038844803172083
1107	10	0.4672728634069969
1107	11	0.10566193839398608
1107	12	-0.5706774097130136
1107	13	-0.34105315509936673
1107	14	0.11826152585285854
1107	15	-0.6760537975389624
1107	16	-0.8451959374122211
1107	17	-0.115109399259687
1107	18	-0.2851580253540007
1107	19	-0.020772076572583842
1107	20	-0.5227363770830706
1107	21	-0.26023212192254164
1107	22	-0.21385170179520685
1107	23	0.07753994358103711
1107	24	-0.09343312623358457
1107	25	-0.4307923557772284
1108	1	0.1978916105125208
1108	2	0.4437925968384562
1108	3	0.4475905753769176
1108	4	0.6291361116498848
1108	5	0.5861263075246015
1108	6	0.3629208667406587
1108	7	0.3861072872125533
1108	8	0.4571975117338809
1108	9	0.7424269848164755
1108	10	0.8527808098932042
1108	11	0.6467233543747071
1108	12	0.4453523286105536
1108	13	0.5095299106790563
1108	14	0.5046593297228128
1108	15	0.7016500539860004
1108	16	0.7255377044706321
1108	17	0.8005802173597942
1108	18	0.8076863399607603
1108	19	0.6100327398376351
1108	20	0.5973444448906022
1108	21	0.5298511892610378
1108	22	0.6851630211079112
1108	23	0.6081857036971456
1108	24	0.4770213584929337
1108	25	0.619189432206781
1109	1	0.2564876732715504
1109	2	0.2903929632493032
1109	3	0.28846577226689263
1109	4	0.6239694952530002
1109	5	0.46259076399655896
1109	6	0.34193742225525625
1109	7	0.3549976236849517
1109	8	0.3557736900016977
1109	9	0.6084639526549864
1109	10	0.3753477524851121
1109	11	0.4986139170611281
1109	12	0.27435687523298213
1109	13	0.17317061579652204
1109	14	0.6654915599137254
1109	15	0.4121893230633802
1109	16	0.4367179132577874
1109	17	0.7070836608398068
1109	18	0.31410725721669763
1109	19	0.37912792566825715
1109	20	0.10990490826627308
1109	21	0.2696509619299192
1109	22	0.2664820722378707
1109	23	0.4084512957076001
1109	24	0.34076162570475077
1109	25	0.20859807805058495
1110	1	0.32764865250003794
1110	2	0.649783534885831
1110	3	0.4083792709511762
1110	4	0.6804332990713746
1110	5	0.5025370855910936
1110	6	0.6048328368457021
1110	7	0.632232590932027
1110	8	0.6120138921180427
1110	9	1.0777220429074958
1110	10	1.0651509549933706
1110	11	0.7308076632119622
1110	12	0.784821014978796
1110	13	0.6405058713813148
1110	14	0.8689589609550978
1110	15	0.6120105116866341
1110	16	0.8435837802305244
1110	17	1.2903030354548903
1110	18	1.3429504770812102
1110	19	0.9369049958211022
1110	20	0.9206839799867836
1110	21	0.8204148469307597
1110	22	0.6626392022977754
1110	23	1.1356124289028315
1110	24	0.920038401921083
1110	25	0.7800585925794508
1111	1	0.28818436773254064
1111	2	0.09134282109370773
1111	3	0.4820291579299685
1111	4	0.7256753624021357
1111	5	0.6664600791774262
1111	6	0.4174498566557858
1111	7	0.3472763413628144
1111	8	0.33341192648568097
1111	9	0.5628143774361334
1111	10	0.3891770196814406
1111	11	0.2760891381608417
1111	12	0.25715211498710744
1111	13	0.15886656014794198
1111	14	0.2417720138002437
1111	15	0.37817979077214403
1111	16	-0.13940029782921926
1111	17	0.37649288634465705
1111	18	0.6000033010208483
1111	19	0.4828435667169515
1111	20	-0.17777896690650152
1111	21	-0.38081027117154775
1111	22	-0.07236025866439633
1111	23	0.3996002837620338
1111	24	0.37321510205522657
1111	25	0.2658013071739447
1112	1	0.1041252330801612
1112	2	-0.0027369455707741963
1112	3	0.33078318672929186
1112	4	0.46876050364877386
1112	5	0.5122008795513082
1112	6	0.20511111549349437
1112	7	0.19744988964144028
1112	8	0.3915149782566374
1112	9	-1.4587502073161218
1112	10	0.028220822811007906
1112	11	-0.03934198255068111
1112	12	0.13077106680109551
1112	13	0.05061938351341411
1112	14	0.28446838125524465
1112	15	0.12737901792660722
1112	16	0.13200149635790837
1112	17	0.34515409958675747
1112	18	0.44130494160778255
1112	19	0.3762193087142123
1112	20	0.22369925691663184
1112	21	0.23696781216012397
1112	22	0.3258336566018656
1112	23	0.03553895482107805
1112	24	0.3028621493122259
1112	25	0.13714653431130586
1113	1	0.20815853871316392
1113	2	0.15810638777201547
1113	3	0.40429743727455403
1113	4	0.513082691615864
1113	5	0.46554307131891903
1113	6	0.1977227842353244
1113	7	0.0715610918264602
1113	8	0.2043420289085418
1113	9	-0.09308807953344456
1113	10	0.34888148994470924
1113	11	0.20472040843243633
1113	12	-0.07924614900340998
1113	13	-3.4131226602340803E-4
1113	14	-0.39402327886450195
1113	15	-0.06362636236682562
1113	16	0.15823113590377602
1113	17	0.3385716069538205
1113	18	0.3082554185167652
1113	19	0.36337587894022505
1113	20	0.4550288303787653
1113	21	0.15036089974517353
1113	22	0.36626775297066416
1113	23	0.04645305164367386
1113	24	0.005024831574274913
1113	25	0.2484320330449421
1114	1	0.42497435232979086
1114	2	0.4355994115437092
1114	3	0.3947205944594998
1114	4	0.6645577443002537
1114	5	0.5353846839423113
1114	6	0.5371193212054735
1114	7	0.4728615092616266
1114	8	0.5443908312890215
1114	9	0.3568806660823114
1114	10	0.5560455383680676
1114	11	0.5263941302897166
1114	12	0.4664730287014578
1114	13	0.49049134713317244
1114	14	0.7943690411510076
1114	15	0.4675736664268726
1114	16	0.33784762147129754
1114	17	0.7960450774538685
1114	18	0.7325632495947119
1114	19	0.6477467035785267
1114	20	0.6071080365541377
1114	21	0.5270603339343154
1114	22	0.3046723764159296
1114	23	0.8974596191042963
1114	24	0.7015069321039462
1114	25	0.4992761952327882
1115	1	0.5665014431613926
1115	2	0.28975498722103765
1115	3	0.5063562366903476
1115	4	0.6976744905187005
1115	5	0.7852341498306004
1115	6	0.6475279397952605
1115	7	0.5099846620905311
1115	8	0.40358797258177503
1115	9	0.8727007774396327
1115	10	0.5540358890177544
1115	11	0.6677627242480284
1115	12	0.6491865558408207
1115	13	0.5834584844369672
1115	14	0.7080663561078069
1115	15	0.621731972193885
1115	16	0.5509538720116325
1115	17	0.9222237557567823
1115	18	1.0788925681794908
1115	19	0.6094033523327695
1115	20	0.8119239147557309
1115	21	0.722321656602098
1115	22	0.2228932983065519
1115	23	0.5881844427480871
1115	24	0.7339987911281796
1115	25	0.8971192727112225
1116	1	0.7405260129420199
1116	2	0.4302493613649041
1116	3	0.9697885596500129
1116	4	1.462527976253547
1116	5	1.1333205210331698
1116	6	0.9438786184194896
1116	7	0.9117800028608869
1116	8	0.9543587451228177
1116	9	1.3606004940137666
1116	10	1.0775352599581467
1116	11	0.8019370272794575
1116	12	0.780670097128386
1116	13	0.6213944242954597
1116	14	1.1270898632353408
1116	15	0.9258687178071412
1116	16	0.9476143489179307
1116	17	1.2659330821277948
1116	18	1.2557420887445474
1116	19	1.158124057624164
1116	20	1.0531128141418762
1116	21	1.0475276329010512
1116	22	1.1029794895561835
1116	23	1.341591525757579
1116	24	1.0002616496184658
1116	25	0.9709263476538581
1201	1	-0.3237826567225568
1201	2	0.09247293789935185
1201	3	-0.15515421194497847
1201	4	-0.26236154398383604
1201	5	-0.28441247979660034
1201	6	-0.417422880609962
1201	7	-0.28651632712724506
1201	8	-0.32166589539317414
1201	9	0.1307921316834637
1201	10	0.33381358008320205
1201	11	0.1538747269253733
1201	12	0.08588887954692126
1201	13	0.1675210788778756
1201	14	0.6969458605107803
1201	15	0.3328140242563862
1201	16	0.08805080092859896
1201	17	0.28474881077142555
1201	18	0.3935200806231549
1201	19	0.08348029277558311
1201	20	0.05541502045092983
1201	21	-0.027720717837929953
1201	22	0.1305992434945793
1201	23	-0.07857214187926331
1201	24	-0.3255522634003327
1201	25	0.03396310901533184
1202	1	-0.20118448241707299
1202	2	-0.1912673011276027
1202	3	-0.5705755789928517
1202	4	-0.35525319144906575
1202	5	-0.5647882044815802
1202	6	-0.5158498334220738
1202	7	-0.15106313216184475
1202	8	-0.27716679694893287
1202	9	0.2305214885266409
1202	10	0.2583242523156386
1202	11	-0.050737851100663534
1202	12	-0.015113241138045908
1202	13	-0.15720730760196489
1202	14	-0.0014705065194462463
1202	15	-0.0745190997137044
1202	16	0.21689176367017637
1202	17	0.5778969870176383
1202	18	0.33275839833069654
1202	19	0.004615864884165433
1202	20	0.047750914367284736
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2211	10	-0.1153586884774569
2211	11	0.25251613401389505
2211	12	0.014823637960041935
2211	13	0.018257637432796514
2211	14	0.3633434614556066
2211	15	0.06347982726696699
2211	16	0.09318631827713375
2211	17	0.3415839246276846
2211	18	0.5617335208457419
2211	19	0.07038654841188219
2211	20	0.2436921647588619
2211	21	0.005737214893983378
2211	22	0.04854719097077975
2211	23	-1.9753132693544018
2211	24	-0.5213886426214174
2211	25	-0.35560342468940576
2212	1	-0.14049762084609796
2212	2	-1.308713585030386
2212	3	-0.30817684704498305
2212	4	0.31490179270028307
2212	5	0.10514487054483368
2212	6	0.1798564011675694
2212	7	0.08111721303012587
2212	8	0.08785609843676667
2212	9	0.6735014110345645
2212	10	0.4589160099767895
2212	11	0.3391494331543541
2212	12	0.06887407834064163
2212	13	-0.05350529985275898
2212	14	0.4075573201800964
2212	15	0.24760136523874365
2212	16	0.23568711414569066
2212	17	0.6766078006844225
2212	18	0.5776685779714157
2212	19	0.3998007891532751
2212	20	0.43938881503652993
2212	21	0.35343980765689054
2212	22	0.3627334595852574
2212	23	0.6767365343313543
2212	24	0.38256240261928554
2212	25	0.40070268404392806
2213	1	0.41243934391594195
2213	2	0.1551934766009495
2213	3	0.3018680778462155
2213	4	0.6114846931635682
2213	5	0.5319762618125594
2213	6	0.3411550113037478
2213	7	0.3746775106575271
2213	8	0.45798104579041166
2213	9	0.6127489191070055
2213	10	0.6770600504091888
2213	11	0.44339202756682283
2213	12	0.37972905517624156
2213	13	0.3323265125858228
2213	14	-1.1283980357092815
2213	15	-0.1069051629095128
2213	16	0.14719630970935782
2213	17	0.6719075339428784
2213	18	0.46311010541771935
2213	19	0.3124696084709768
2213	20	0.43471803025483524
2213	21	0.4789557419542376
2213	22	0.46807109822066906
2213	23	0.7089139876515844
2213	24	0.25585315089950456
2213	25	0.1818145751010647
2214	1	0.33441817119686235
2214	2	0.4313725407062172
2214	3	0.44697674966340983
2214	4	0.6014051575316941
2214	5	0.6178845058180329
2214	6	0.5089261037373944
2214	7	0.3169671380623811
2214	8	0.1379519874147661
2214	9	0.5067933668210287
2214	10	0.43779672408815884
2214	11	0.2634044629671095
2214	12	0.2936050661714589
2214	13	0.2681826948471979
2214	14	0.5893792107620541
2214	15	0.2576540170048389
2214	16	0.4937379527383925
2214	17	0.4713516899413719
2214	18	0.7065956674794194
2214	19	0.35329881907063765
2214	20	0.5818812310900867
2214	21	0.44911608646716417
2214	22	0.506753822917311
2214	23	0.49636413640952526
2214	24	0.6215037883021921
2214	25	0.6698902428848896
2215	1	0.6947674967651594
2215	2	0.31298882447876153
2215	3	0.4020537211409021
2215	4	0.9827643826540964
2215	5	0.4961992545625904
2215	6	0.6411614027786854
2215	7	0.4794080010434456
2215	8	0.5162081087478967
2215	9	0.7230599660451276
2215	10	0.8139509174272916
2215	11	0.46525268760282046
2215	12	0.49346825685197615
2215	13	0.3710555823211692
2215	14	0.48957479180660357
2215	15	0.42867973694043116
2215	16	0.5368206217179221
2215	17	0.7273020114180184
2215	18	0.9102918281235759
2215	19	0.6681381204339303
2215	20	-0.007639218331412678
2215	21	0.3511520270546882
2215	22	-0.5610057549075975
2215	23	0.49693273210443323
2215	24	0.41984714000785317
2215	25	0.5682108291157135
2216	1	0.4831029280290693
2216	2	-0.11457391074446477
2216	3	0.5070959655791469
2216	4	0.8062609809427954
2216	5	0.6685587296428039
2216	6	0.4195967687694946
2216	7	0.5087696883849091
2216	8	0.44982121597549707
2216	9	0.7519927265294584
2216	10	0.6192973337770269
2216	11	0.5983690915517883
2216	12	0.25509514503619357
2216	13	0.5478463052460043
2216	14	0.6861652716479725
2216	15	0.6085626189851752
2216	16	0.6448836620097751
2216	17	0.6260831193570127
2216	18	1.0482026479529978
2216	19	0.7547204439396822
2216	20	-0.31155257809530923
2216	21	-0.9630914457284447
2216	22	-0.7447261872569015
2216	23	-1.3496347508309654
2216	24	-0.8690463071629679
2216	25	-0.5563980273271767
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2137.0
3	266.0
4	237.0
5	180.0
6	231.0
7	243.0
8	214.0
9	413.0
10	905.0
11	1738.0
12	3196.0
13	5243.0
14	7492.0
15	9795.0
16	11941.0
17	12520.0
18	12490.0
19	11929.0
20	11016.0
21	10497.0
22	9784.0
23	9632.0
24	9427.0
25	9626.0
26	10248.0
27	11014.0
28	12387.0
29	14747.0
30	18144.0
31	22724.0
32	29987.0
33	39299.0
34	53788.0
35	75611.0
36	111976.0
37	179538.0
38	316237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9959703980729	21.84281848053486	12.763754445408166	21.397456675984074
2	14.814169974696473	24.38985228002569	38.461263164411015	22.334714580866823
3	18.151156241108257	31.34267978499161	29.002518238355808	21.503645735544325
4	13.335534124198178	21.053243004098892	34.026711534403006	31.58451133729992
5	12.509233845006948	41.100412921089955	32.03473765230399	14.355615581599112
6	27.680689167311	39.99040027560499	17.929033650324232	14.399876906759774
7	23.48803765704144	37.39700733360043	21.461378113005964	17.65357689635217
8	23.489619780732447	37.6367034564596	21.138015441840476	17.735661320967473
9	25.326967855822513	20.431208518100576	19.942087445634396	34.299736180442515
10	16.51145410613054	31.418636349555467	28.217546699296804	23.85236284501719
11	29.35108652018514	30.013010190509814	20.970492501003573	19.665410788301468
12	21.262619224575747	31.28029000990957	27.659714480366652	19.797376285148026
13	26.369112232083946	26.629748090702055	26.572860976794317	20.42827870041969
14	22.413954924000894	27.115846265937364	25.459531165487338	25.010667644574404
15	25.574572458465454	30.26833385602391	23.222948826656445	20.934144858854182
16	22.261210953221262	32.02280889136559	24.983262902077335	20.73271725333581
17	19.920784949140334	32.40760905862437	26.469093643921376	21.20251234831392
18	20.016933572016065	30.153176254293868	29.808989307658813	20.020900866031255
19	21.49633648408928	31.360586549656098	27.20689211330334	19.936184852951282
20	23.158698188445754	28.509003596795196	27.54169980013505	20.790598414624
21	24.118128105992053	30.367130075439015	25.08281838989243	20.4319234286765
22	22.43640218928535	29.54645266512021	26.021269886105316	21.99587525948913
23	22.79609182773402	28.978872966992153	26.421848160814598	21.803187044459232
24	21.837634706147576	28.910759656561	26.422916831955227	22.828688805336192
25	23.254587741770703	29.22348939266116	26.064990750988752	21.456932114579384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12562.0
1	12562.0
2	9477.0
3	6392.0
4	6392.0
5	6392.0
6	7215.5
7	8039.0
8	8039.0
9	8039.0
10	8164.0
11	8289.0
12	8289.0
13	8289.0
14	9636.5
15	10984.0
16	10984.0
17	10984.0
18	14409.5
19	17835.0
20	17835.0
21	17835.0
22	23943.0
23	30051.0
24	30051.0
25	30051.0
26	38693.0
27	47335.0
28	47335.0
29	47335.0
30	57253.5
31	67172.0
32	67172.0
33	67172.0
34	75291.0
35	83410.0
36	83410.0
37	83410.0
38	94883.0
39	106356.0
40	106356.0
41	106356.0
42	132095.5
43	157835.0
44	157835.0
45	157835.0
46	148196.0
47	138557.0
48	138557.0
49	138557.0
50	128451.0
51	118345.0
52	118345.0
53	118345.0
54	102035.0
55	85725.0
56	85725.0
57	85725.0
58	73818.0
59	61911.0
60	61911.0
61	61911.0
62	51153.5
63	40396.0
64	40396.0
65	40396.0
66	30032.5
67	19669.0
68	19669.0
69	19669.0
70	14932.5
71	10196.0
72	10196.0
73	10196.0
74	7148.5
75	4101.0
76	4101.0
77	4101.0
78	2612.5
79	1124.0
80	1124.0
81	1124.0
82	765.5
83	407.0
84	407.0
85	407.0
86	259.5
87	112.0
88	112.0
89	112.0
90	73.0
91	34.0
92	34.0
93	34.0
94	22.0
95	10.0
96	10.0
97	10.0
98	7.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1460189110885642
2	0.28972312345445633
3	0.3462403505996999
4	0.375174084633101
5	0.3826004097016739
6	0.33649932680845485
7	0.3400678206725743
8	0.3554026997102769
9	0.34431143499747313
10	0.37228071122976086
11	0.29387029199924386
12	0.3383317966305702
13	0.3111340866391732
14	0.323382700713313
15	0.36032143449595505
16	0.31055541195850517
17	0.358296073113617
18	0.3281085439387685
19	0.34286474829580305
20	0.3041899904711569
21	0.3440220976571391
22	0.2980174605440314
23	0.2812358948046587
24	0.31884974904808017
25	0.31132697819939587
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1036852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.64456134845915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6466911229577	19.62283779736669
2	7.883944723771898	4.201284977208024
3	3.772060347433983	3.0151468001188473
4	2.4188649095038444	2.5779837789964115
5	1.7066235569634707	2.2736118031119386
6	1.3470125746654364	2.1534335509691482
7	1.0278622200107135	1.917085658518721
8	0.872537839497591	1.8598710394676456
9	0.7141109479278583	1.7124455665503124
>10	5.8958304059380975	31.10907044200993
>50	0.5071906704871673	9.154209531797019
>100	0.17907307938496547	8.503981854290398
>500	0.0146481046531665	2.5437302156083654
>1k	0.012084686338862363	6.193158582473885
>5k	0.0010986078489874877	2.0846488705973427
>10k+	3.6620261632916255E-4	1.0774995309153501
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	11043	1.0650507497694945	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9176	0.8849864783016285	No Hit
GCTTATGTACTCTGCGTTGATACCA	6606	0.6371208234154923	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5583	0.5384567903615944	No Hit
CTTATGTACTCTGCGTTGATACCAC	4647	0.4481835401773831	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4085	0.39398101175481165	No Hit
GTGGTATCAACGCAGAGTACATGGG	3901	0.37623498821432566	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3555	0.34286474829580305	No Hit
GAGTACATAAGCAGTGGTATCAACG	3121	0.3010072797274828	No Hit
CCCATGTACTCTGCGTTGATACCAC	3068	0.2958956533815819	No Hit
TATGTACTCTGCGTTGATACCACTG	2775	0.2676370398089602	No Hit
GCGTTGATACCACTGCTTATGTACT	2246	0.21661722213006293	No Hit
CATGTACTCTGCGTTGATACCACTG	2100	0.20253613823380773	No Hit
CATAAGCAGTGGTATCAACGCAGAG	2064	0.19906409014979956	No Hit
ACTCTGCGTTGATACCACTGCTTAT	1939	0.18700836763588247	No Hit
CTGCTTATGTACTCTGCGTTGATAC	1769	0.17061258501695517	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1743	0.1681049947340604	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	1735	0.1673334284931697	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1684	0.1624146937074915	No Hit
GTATCAACGCAGAGTACATAAGCAG	1588	0.15315589881680317	No Hit
GTACTGGTTCACTATCGGTCAGTCA	1524	0.1469833688896776	No Hit
GTACATGATAAGCAGTGGTATCAAC	1509	0.14553668218800755	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1488	0.14351132080566947	No Hit
ATCATGTACTCTGCGTTGATACCAC	1439	0.13878547758021395	No Hit
GGTATCAACGCAGAGTACATGGGGG	1351	0.1302982489304163	No Hit
ATTCCATTCCATTCCATTCCATTCC	1339	0.12914089956908026	No Hit
GGTATCAACGCAGAGTACATAAGCA	1303	0.12566885148507212	No Hit
TATCAACGCAGAGTACATAAGCAGT	1270	0.12248614074139799	No Hit
GAATGGAATGGAATGGAATGGAATG	1246	0.12017144201872591	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	1202	0.11592782769382709	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	1164	0.11226288804959626	No Hit
ATACAGGGTGACAGCCCCGTACACA	1143	0.11023752666725821	No Hit
GGTATCAACGCAGAGTACATGGGGA	1130	0.10898373152581083	No Hit
GGATACCACGTGTCCCGCCCTACTC	1127	0.10869439418547681	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1116	0.10763349060425209	No Hit
GGTATCAACGCAGAGTACATGGGAA	1078	0.1039685509600213	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.644578011133701E-5	0.0	0.0	3.8578312044534803E-4	0.0
2	9.644578011133701E-5	0.0	0.0	3.8578312044534803E-4	0.0
3	9.644578011133701E-5	0.0	0.0	3.8578312044534803E-4	0.0
4	9.644578011133701E-5	0.0	0.0	3.8578312044534803E-4	0.0
5	9.644578011133701E-5	0.0	0.0	3.8578312044534803E-4	0.0
6	1.9289156022267402E-4	0.0	0.0	3.8578312044534803E-4	0.0
7	1.9289156022267402E-4	0.0	0.0	3.8578312044534803E-4	0.0
8	1.9289156022267402E-4	0.0	0.0	3.8578312044534803E-4	0.0
9	1.9289156022267402E-4	0.0	0.0	3.8578312044534803E-4	0.0
10	2.8933734033401105E-4	0.0	0.0	4.8222890055668507E-4	0.0
11	2.8933734033401105E-4	0.0	0.0	5.786746806680221E-4	0.0
12	2.8933734033401105E-4	0.0	0.0	7.715662408906961E-4	0.0
13	2.8933734033401105E-4	0.0	0.0	7.715662408906961E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	190	0.0	16.009048	1
GTGCGGT	70	4.4374246E-7	14.926899	11
GACCGAA	45	6.7307043E-4	14.786134	1
GTCCTTA	40	0.005257762	14.258059	1
CATCTAG	60	2.5529715E-5	14.258058	1
CAGGAAC	40	0.0052645192	14.2553	3
AGTACGG	40	0.0052645192	14.2553	3
TCTAACC	70	7.2291023E-6	13.576475	3
GTCCGTG	70	7.26532E-6	13.569908	10
GAGCCAG	65	5.4547734E-5	13.152372	10
TCTGCAC	75	1.471812E-5	12.671377	3
TCCGTGA	75	1.479152E-5	12.665248	11
ATCGCCG	75	1.4813602E-5	12.663411	19
GTCCTAC	70	1.0844799E-4	12.221193	1
CAGTCTA	70	1.0913597E-4	12.212917	9
TTATCCG	55	0.0030587215	12.095405	4
TAGGACC	55	0.0030587215	12.095405	4
GCGTGAG	55	0.003069825	12.089555	9
GGGGCAT	55	0.0030709372	12.08897	7
GGTGCGG	80	2.8674707E-5	11.873671	10
>>END_MODULE