FastQCFastQC Report
Wed 25 May 2016
SRR1295165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036852
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT163501.57688850482036No Hit
GTACATAAGCAGTGGTATCAACGCA119131.1489585784663576No Hit
GGTATCAACGCAGAGTACTTTTTTT115101.110090929081489No Hit
TATCAACGCAGAGTACTTTTTTTTT103741.00052852287501No Hit
GCTTATGTACTCTGCGTTGATACCA69670.6719377500356849No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55620.5364314289792564No Hit
CTTATGTACTCTGCGTTGATACCAC50750.4894623340650353No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46270.44625462457515636No Hit
GTGGTATCAACGCAGAGTACATGGG41400.3992855296609353No Hit
CCCATGTACTCTGCGTTGATACCAC38070.36716908488386No Hit
GAGTACATAAGCAGTGGTATCAACG33900.32695119457743244No Hit
CTTATACACATCTCCGAGCCCACGA33540.32347914649342435No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27290.2632005339238387No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA27150.26185029300227997No Hit
CATAAGCAGTGGTATCAACGCAGAG26030.2510483656298102No Hit
TATGTACTCTGCGTTGATACCACTG25600.24690119708502276No Hit
GCGTTGATACCACTGCTTATGTACT25560.24651541396457738No Hit
CATGTACTCTGCGTTGATACCACTG23710.22867294464398005No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21530.20764776457970857No Hit
CTGCTTATGTACTCTGCGTTGATAC21140.20388637915536642No Hit
ACTCTGCGTTGATACCACTGCTTAT20910.2016681262128057No Hit
ACGCAGAGTACATAAGCAGTGGTAT20260.19539915050556877No Hit
ATCAACGCAGAGTACTTTTTTTTTT16380.15797818782237003No Hit
GTACTGGTTCACTATCGGTCAGTCA15830.15267366991624648No Hit
GTACATGATAAGCAGTGGTATCAAC15750.15190210367535578No Hit
GGTATCAACGCAGAGTACATGGGGG15740.15180565789524444No Hit
GTATCAACGCAGAGTACATAAGCAG15630.15074475431401974No Hit
GTGGTATCAACGCAGAGTACTTTTT15630.15074475431401974No Hit
GTACATGGGAAGCAGTGGTATCAAC15420.14871939293168165No Hit
ATTCCATTCCATTCCATTCCATTCC15190.1465011399891209No Hit
ATCATGTACTCTGCGTTGATACCAC14840.14312553768522412No Hit
ATACACATCTCCGAGCCCACGAGAC14760.14235397144433343No Hit
GGTATCAACGCAGAGTACATAAGCA13470.12991246580997096No Hit
AAGCAGTGGTATCAACGCAGAGTAC13300.12827288754807822No Hit
TATCAACGCAGAGTACATAAGCAGT13220.12750132130718753No Hit
GGTATCAACGCAGAGTACATGGGGA13100.12634397194585148No Hit
GAATGGAATGGAATGGAATGGAATG13020.12557240570496078No Hit
ATACAGGGTGACAGCCCCGTACACA12980.12518662258451543No Hit
GGATACCACGTGTCCCGCCCTACTC12070.11641005659438378No Hit
GGTATCAACGCAGAGTACATGGGAA11660.11245577960981895No Hit
GTGGTATCAACGCAGAGTACATAAG11210.10811571950480878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG350.00216711816.2884431
TACCATA350.002169912716.2853017
CAAGCGG350.002169912716.28530117
CTTATAC4550.015.87079051
TATACAC4850.015.4745793
GGACGAT508.711368E-515.1996147
TTATACA5100.014.9023012
TATGGAC400.005276734514.2503263
CCGCTAA400.005276734514.25032613
CCGTCTG400.005276734514.25032614
TCCGCTA400.00527842314.24963912
CTAACAT759.661289E-713.9336513
ACGATCA551.9595832E-413.8178329
GAGTTAT551.9595832E-413.81783218
TACATCG500.001498702213.3003034
TCTATGG500.001498702213.3003033
ATTAACG500.00149924713.29966317
CACGACA801.9970594E-613.06279854
GGTGCGG801.9981399E-613.06216910
GTCCTAG905.388192E-712.6687891
Produced by FastQC (version 0.11.5)