FastQCFastQC Report
Wed 25 May 2016
SRR1295164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739285
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA157302.127731524378284No Hit
GTACATAAGCAGTGGTATCAACGCA141631.9157699669275041No Hit
GCTTATGTACTCTGCGTTGATACCA86091.164503540583131No Hit
GTATCAACGCAGAGTACTTTTTTTT71410.9659333004186477No Hit
CTTATGTACTCTGCGTTGATACCAC59710.8076722779442299No Hit
GGTATCAACGCAGAGTACTTTTTTT51160.6920199922898477No Hit
TATCAACGCAGAGTACTTTTTTTTT43600.5897590239217623No Hit
GAGTACATAAGCAGTGGTATCAACG41250.5579715535957039No Hit
GTGGTATCAACGCAGAGTACATGGG37840.5118459051651257No Hit
TATGTACTCTGCGTTGATACCACTG34360.46477339591632455No Hit
GCGTTGATACCACTGCTTATGTACT29380.39741101199131595No Hit
CCCATGTACTCTGCGTTGATACCAC27920.3776622006398074No Hit
CATAAGCAGTGGTATCAACGCAGAG26200.3543964776777562No Hit
GTACTGGTTCACTATCGGTCAGTCA23050.3117877408577206No Hit
ACGCAGAGTACATAAGCAGTGGTAT23050.3117877408577206No Hit
CTGCTTATGTACTCTGCGTTGATAC22890.3096234875589252No Hit
ACTCTGCGTTGATACCACTGCTTAT22150.29961381605199616No Hit
GTATCAACGCAGAGTACATAAGCAG19500.26376837079069637No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19360.2618746491542504No Hit
ATACAGGGTGACAGCCCCGTACACA19110.2584930033748825No Hit
TATCAACGCAGAGTACATAAGCAGT17710.2395557870104222No Hit
GGTATCAACGCAGAGTACATAAGCA17220.23292776128286113No Hit
CATGTACTCTGCGTTGATACCACTG16920.22886978634761965No Hit
GGATACCACGTGTCCCGCCCTACTC16320.2207538364771367No Hit
GGTATCAACGCAGAGTACATGGGGG14600.19748811351508552No Hit
AAGCAGTGGTATCAACGCAGAGTAC13330.18030935295589656No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13070.17679244134535396No Hit
ACCCTGTATCGCGCGCCTTTCCAGA12500.16908228896839514No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA12490.1689470231372204No Hit
GTGGTATCAACGCAGAGTACATAAG12370.16732383316312383No Hit
ACATAAGCAGTGGTATCAACGCAGA11820.15988421244851445No Hit
GTACATGGGAAGCAGTGGTATCAAC11590.15677309833149597No Hit
GTACATGATAAGCAGTGGTATCAAC9760.13201945122652292No Hit
GATACCACTGCTTATGTACTCTGCG9710.13134312207064933No Hit
ATCATGTACTCTGCGTTGATACCAC9640.13039626125242634No Hit
CCCATATTCAGACAGGATACCACGT9570.12944940043420333No Hit
CCACTGCTTATGTACTCTGCGTTGA9460.12796147629128143No Hit
GTATTTAGCCTTGGAGGATGGTCCC9290.12566195716131126No Hit
GTTCACTATCGGTCAGTCAGGAGTA9250.1251208938366124No Hit
GGTATCAACGCAGAGTACATGGGCA9170.12403876718721468No Hit
GTACTCTGCGTTGATACCACTGCTT9010.12187451388841922No Hit
TCTAAGTACCCCGAGGAAAAGAAAT8940.1209276530701962No Hit
GCAGTGGTATCAACGCAGAGTACAT8900.12038658974549733No Hit
GCCTTGGAGGATGGTCCCCCCATAT8630.11673441230378001No Hit
GTTGATACCACTGCTTATGTACTCT8570.11592281731673171No Hit
ATACCACTGCTTATGTACTCTGCGT8240.11145904488796608No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN8170.11051218406974306No Hit
GGTATCAACGCAGAGTACATGGGGA8150.11024165240739363No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7710.10428995583570612No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7590.10266676586160953No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG7580.1025315000304348No Hit
TATTCAGACAGGATACCACGTGTCC7510.1015846392122118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATAA250.006033860618.99742110
GTGGTCT250.006033860618.9974219
CCTTACT607.680683E-817.41430513
CCCCTAT350.002160889316.294561
TGGACTG350.002160889316.294565
ACGCGCA350.00217071116.2835049
ACCCTTA651.9122308E-716.07474111
TTACAGC508.663795E-515.2082584
CTTACTT704.4370063E-714.92553214
GTTACAG602.5515415E-514.2577423
CTCTATA400.00525664114.257741
CTGGACT400.00525664114.257744
ACTATGG400.00528037114.2480668
ACGCGTT400.005282748514.24709919
AATCGCC400.00528750714.24516618
AACGGTT551.9602067E-413.8163077
TTAGCAC500.001492121813.3072263
AGGTTTA500.001499775213.2981959
AACGCGT500.001502077813.29548818
TCGAACG500.001502077813.29548818
Produced by FastQC (version 0.11.5)