##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739285 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.628595196710336 31.0 28.0 31.0 16.0 34.0 2 28.692076803938942 31.0 28.0 33.0 16.0 34.0 3 28.83156563436293 31.0 28.0 34.0 16.0 34.0 4 31.73868129341188 35.0 32.0 37.0 17.0 37.0 5 32.41111614600594 35.0 32.0 37.0 20.0 37.0 6 32.52715529193748 35.0 32.0 37.0 19.0 37.0 7 32.52094523762825 35.0 32.0 37.0 19.0 37.0 8 32.65044333376167 35.0 32.0 37.0 21.0 37.0 9 33.12741770764996 37.0 32.0 39.0 17.0 39.0 10 33.35878179592444 37.0 32.0 39.0 17.0 39.0 11 33.68759815226875 38.0 32.0 39.0 17.0 39.0 12 33.7622892389268 38.0 32.0 39.0 18.0 39.0 13 33.49664743637433 38.0 32.0 39.0 17.0 39.0 14 33.96069716009387 38.0 32.0 41.0 17.0 41.0 15 34.277315243782844 38.0 32.0 41.0 17.0 41.0 16 34.224146303523 38.0 32.0 40.0 17.0 41.0 17 34.00683092447432 38.0 32.0 40.0 17.0 41.0 18 33.925191232068826 38.0 32.0 40.0 17.0 41.0 19 34.35102835848151 39.0 32.0 40.0 18.0 41.0 20 34.39383728873168 39.0 32.0 41.0 18.0 41.0 21 34.46261455325077 39.0 32.0 41.0 18.0 41.0 22 34.38365718227747 39.0 32.0 41.0 18.0 41.0 23 34.15572343548158 38.0 32.0 40.0 18.0 41.0 24 34.485358150104496 38.0 32.0 41.0 21.0 41.0 25 33.282132060030975 38.0 30.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1550008458100116 1101 2 0.48087539373225496 1101 3 0.17264830152484478 1101 4 0.20146921968315823 1101 5 -0.04252533710272388 1101 6 -0.038341544026152974 1101 7 -0.19703036202982105 1101 8 0.10459959813128705 1101 9 0.5310307744886344 1101 10 0.39727396155212347 1101 11 0.31535867073036883 1101 12 0.5372723295798636 1101 13 0.24610772000487913 1101 14 0.7048958598749806 1101 15 0.23326166139893445 1101 16 0.4724809412434041 1101 17 0.6288277954759565 1101 18 0.5210821960473737 1101 19 0.051912838951558626 1101 20 0.26588111126967107 1101 21 0.23231354762570078 1101 22 0.035370866284580416 1101 23 0.02456520000825435 1101 24 -0.11561533052325501 1101 25 -0.0015209399353537378 1102 1 -0.05624885582254535 1102 2 0.10918370385042309 1102 3 -0.1269007175698782 1102 4 -0.2592751406765146 1102 5 -0.4661071897008817 1102 6 -0.31075954551540974 1102 7 -0.3903875330385773 1102 8 -0.3251890903128327 1102 9 -0.22416121554490331 1102 10 0.29945860599725904 1102 11 -0.03908678611479388 1102 12 -0.21478888392117312 1102 13 -0.3555683715045177 1102 14 0.008616868670181077 1102 15 -0.18108249722073566 1102 16 -0.3163034562605418 1102 17 -0.47065683176101203 1102 18 -0.0013419512164816183 1102 19 -0.4831795340232148 1102 20 -0.1700430427347186 1102 21 -0.34917823408589754 1102 22 -0.5758323653619541 1102 23 -0.5594871889534616 1102 24 -0.5881318230067762 1102 25 -0.646764625845826 1103 1 -0.21774290633155857 1103 2 -0.08934284510355184 1103 3 -0.2774714685040678 1103 4 -0.0019835047099761027 1103 5 -0.3682263904789451 1103 6 -0.2528072686387759 1103 7 -0.29772640196053857 1103 8 -0.24310534915252902 1103 9 0.36952756193591085 1103 10 0.3098126282440532 1103 11 -0.09455642439877465 1103 12 -0.13087942228379745 1103 13 -0.24806957753482095 1103 14 0.002694523061450127 1103 15 -0.09170750788499049 1103 16 -0.1963379541563839 1103 17 -0.33131728653138026 1103 18 0.11784564195032488 1103 19 -0.3752177174856257 1103 20 -0.30277230095240526 1103 21 -0.3186862202557208 1103 22 -0.2760233136973227 1103 23 -2.4845861144618944 1103 24 -0.9591579337568064 1103 25 -0.5156478030027571 1104 1 -0.1415500708232038 1104 2 0.16673974348660892 1104 3 -0.12127171529762037 1104 4 -0.34065624150091267 1104 5 -0.5941110456535093 1104 6 -0.35238161208067 1104 7 -0.5157850216616424 1104 8 -0.3902700277600175 1104 9 -2.6396025531097678 1104 10 -0.921980115028326 1104 11 -0.693231778697097 1104 12 -0.47852023794895615 1104 13 -0.47199889237110426 1104 14 -0.1538479989692405 1104 15 -0.3182356336250578 1104 16 -0.08892822700037328 1104 17 -0.06601889068947742 1104 18 0.29951311879784726 1104 19 -0.043339593615208116 1104 20 -0.43684632655411804 1104 21 -0.2868554608046452 1104 22 -0.5564907880519812 1104 23 -0.2520735566586083 1104 24 0.04948139865416579 1104 25 -0.42000127867864023 1105 1 -0.310072529452043 1105 2 -0.06034772696256141 1105 3 -0.003914741302349256 1105 4 0.27985676086315436 1105 5 0.20534049098952067 1105 6 -0.02482132839272566 1105 7 -0.045602988564716895 1105 8 0.16951484221634416 1105 9 -1.321039905355633 1105 10 -0.14663259729783107 1105 11 0.13780131822962005 1105 12 0.35322680696830133 1105 13 0.47382380266435575 1105 14 0.3824687859007341 1105 15 0.08866507559480397 1105 16 0.026067044867239986 1105 17 -0.812976337497993 1105 18 0.3328653646607336 1105 19 0.05515213215311121 1105 20 0.005666675809948174 1105 21 0.018359669218995123 1105 22 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6 0.24479099926339387 1110 7 0.0962039862933537 1110 8 0.232171776522911 1110 9 0.6147330062420977 1110 10 0.5619545884280441 1110 11 0.19895946908120266 1110 12 0.12160961313212937 1110 13 0.0918546218046643 1110 14 0.3121103106371521 1110 15 0.14266020941474977 1110 16 0.2047142468137153 1110 17 0.5588387476601611 1110 18 0.8889334911814117 1110 19 0.433455457376148 1110 20 0.3417834641311188 1110 21 0.07661108852150988 1110 22 0.3468391803696136 1110 23 0.5660184521074214 1110 24 0.35626859818480483 1110 25 0.1547105468291221 1111 1 0.25880541252934464 1111 2 0.372160209545374 1111 3 0.22454283943109488 1111 4 0.5999727980826393 1111 5 0.8176382468734786 1111 6 0.3790144679947929 1111 7 0.43853056370677024 1111 8 0.34968123521032624 1111 9 0.6473519939328227 1111 10 0.674096466741581 1111 11 0.4378297801891122 1111 12 0.44064005272637985 1111 13 0.46025401612905625 1111 14 -0.0025273826816416545 1111 15 0.018464747187579178 1111 16 -0.0036165895060449316 1111 17 0.599673908807425 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2213 19 -0.026398557222393038 2213 20 0.10739579178724057 2213 21 0.19826558973804964 2213 22 0.2259957091611824 2213 23 0.39651318041237005 2213 24 0.34734726809558936 2213 25 0.09145020619651234 2214 1 0.29956422268470817 2214 2 0.10018218756735209 2214 3 0.1326835492959404 2214 4 0.29389094889734224 2214 5 0.1997037388527687 2214 6 0.2636337998124034 2214 7 -0.003117945829671953 2214 8 -0.1341154996639986 2214 9 0.07408402626830224 2214 10 0.014207146291916217 2214 11 0.08602354045184057 2214 12 -0.3139505434590504 2214 13 -0.3317915819510233 2214 14 0.16726152094346958 2214 15 -0.2168990269693012 2214 16 -0.12721927991908188 2214 17 0.23613263686097952 2214 18 0.4310427150880827 2214 19 0.16328888428986232 2214 20 0.39641881256208933 2214 21 0.5002592418685694 2214 22 0.5733680499185567 2214 23 0.30386274994259566 2214 24 0.41808819079818704 2214 25 0.32427040708240895 2215 1 0.3413052483257353 2215 2 0.10774116192632732 2215 3 0.19599636980876056 2215 4 0.49310120530759605 2215 5 0.3819627132117418 2215 6 0.35597867160727503 2215 7 0.13848590032417718 2215 8 -0.12372960222810292 2215 9 0.1586045390888131 2215 10 0.1624340693688353 2215 11 0.020823540451843314 2215 12 -0.4313954152539168 2215 13 -0.06724286400230994 2215 14 0.2862910081229586 2215 15 -0.042534924405195795 2215 16 -0.12095517735497907 2215 17 0.02462366250200887 2215 18 0.3433542535496201 2215 19 0.2681299099308916 2215 20 -0.5064222130789418 2215 21 -0.059595886336559545 2215 22 -1.084892206491702 2215 23 -0.12320263467279347 2215 24 -0.05847462971463102 2215 25 0.2570242532362528 2216 1 0.242849281247544 2216 2 -0.09768106029589418 2216 3 0.5414679747470323 2216 4 0.7323999707396993 2216 5 0.5432499560100936 2216 6 0.45366426831509443 2216 7 0.368421702793313 2216 8 0.09369591217519257 2216 9 0.5372646213933407 2216 10 0.27331801998612093 2216 11 0.38293712069875596 2216 12 -0.06020529179713208 2216 13 -0.09625027140971554 2216 14 0.5668638476291292 2216 15 0.35325766818739623 2216 16 0.36233041935283694 2216 17 0.32697427978595783 2216 18 0.8632028132204042 2216 19 0.25765418976628496 2216 20 -0.6129670690460145 2216 21 -0.9120765447727734 2216 22 -0.762337473981411 2216 23 -1.3778462560719689 2216 24 -1.036143765517103 2216 25 -0.7346168990271167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1468.0 3 191.0 4 199.0 5 143.0 6 160.0 7 199.0 8 73.0 9 164.0 10 410.0 11 1047.0 12 2410.0 13 4445.0 14 6986.0 15 9838.0 16 12066.0 17 13210.0 18 12692.0 19 11878.0 20 11015.0 21 9974.0 22 9366.0 23 8558.0 24 8156.0 25 8138.0 26 8258.0 27 8623.0 28 9469.0 29 10584.0 30 13117.0 31 16414.0 32 21325.0 33 27779.0 34 37618.0 35 53139.0 36 79607.0 37 127475.0 38 193091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.10441331852294 21.062014033756874 12.525669854514915 20.307902793205276 2 15.330303852414543 26.085594139989148 35.298290830168206 23.285811177428105 3 20.180601062486936 32.12828566076969 27.229010182392688 20.462103094350688 4 12.740555298359846 23.527886425994836 30.531634846051396 33.199923429593916 5 11.487046083412308 44.92012588692443 28.51056434597724 15.08226368368602 6 26.71730963419798 39.43392436399961 18.127096928305413 15.721669073496997 7 22.44028762917661 41.36243827889389 17.558517987681565 18.638756104247932 8 22.14796624135773 39.62961605449601 19.34049285880096 18.881924845345292 9 25.336824416953746 24.95079635255058 16.88343418384383 32.82894504665184 10 16.161780739521767 34.37134675622502 24.710150535707253 24.756721968545953 11 26.77057359991535 33.6115278904514 17.948432713726405 21.669465795906852 12 23.089034300132607 34.79025084118187 21.872628980089882 20.24808587859564 13 27.003919982957775 28.526186815885634 24.550436706997218 19.919456494159373 14 23.555123714711826 31.49612496726137 22.381418298622467 22.56733301940434 15 26.963334437499658 31.602190593588958 21.83045435539313 19.604020613518255 16 21.041048755403473 33.29923232016238 23.4142350473389 22.245483877095246 17 19.10152018267171 34.93854378500778 23.19982297569174 22.76011305662877 18 18.48942606387382 30.234006777416035 29.897578776943757 21.37898838176639 19 20.980708595689464 33.63936081699023 24.989514803468733 20.390415783851576 20 22.815060436302755 30.399412799824166 27.203052138437055 19.582474625436024 21 24.4724690322423 32.160861314938636 22.843387165516997 20.523282487302065 22 22.41589359410231 30.693174079028413 23.413720217826025 23.47721210904325 23 24.02089516605987 28.748894471543835 24.13782345185322 23.092386910543077 24 22.435797401363775 29.538012687858327 23.823319876513892 24.202870034264002 25 23.25573821655619 31.263120042879727 24.597900793139242 20.88324094742484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18577.0 1 18577.0 2 12893.5 3 7210.0 4 7210.0 5 7210.0 6 7831.0 7 8452.0 8 8452.0 9 8452.0 10 8546.0 11 8640.0 12 8640.0 13 8640.0 14 9212.0 15 9784.0 16 9784.0 17 9784.0 18 12258.5 19 14733.0 20 14733.0 21 14733.0 22 17745.5 23 20758.0 24 20758.0 25 20758.0 26 25414.0 27 30070.0 28 30070.0 29 30070.0 30 33135.0 31 36200.0 32 36200.0 33 36200.0 34 38756.0 35 41312.0 36 41312.0 37 41312.0 38 45105.0 39 48898.0 40 48898.0 41 48898.0 42 83163.0 43 117428.0 44 117428.0 45 117428.0 46 108731.0 47 100034.0 48 100034.0 49 100034.0 50 94381.0 51 88728.0 52 88728.0 53 88728.0 54 77700.5 55 66673.0 56 66673.0 57 66673.0 58 58715.5 59 50758.0 60 50758.0 61 50758.0 62 43706.5 63 36655.0 64 36655.0 65 36655.0 66 27089.0 67 17523.0 68 17523.0 69 17523.0 70 14063.0 71 10603.0 72 10603.0 73 10603.0 74 7401.0 75 4199.0 76 4199.0 77 4199.0 78 2799.5 79 1400.0 80 1400.0 81 1400.0 82 926.5 83 453.0 84 453.0 85 453.0 86 295.5 87 138.0 88 138.0 89 138.0 90 91.5 91 45.0 92 45.0 93 45.0 94 28.0 95 11.0 96 11.0 97 11.0 98 7.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14405811020107265 2 0.2820292579992831 3 0.34127569205380875 4 0.36629987082113125 5 0.37211630156164405 6 0.33721771711856724 7 0.33857037543031443 8 0.3576428576259494 9 0.3450631353267008 10 0.3763095423280602 11 0.29095680285681436 12 0.34141095788498343 13 0.31002928505244937 14 0.3230148048452221 15 0.3626476933794139 16 0.3055655126236837 17 0.360212908418269 18 0.3281549064298613 19 0.3424930845343812 20 0.3013722718572675 21 0.3450631353267008 22 0.2952853094544053 23 0.28148819467458425 24 0.31922736157233 25 0.3165220449488357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 739285.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.616206075978855 #Duplication Level Percentage of deduplicated Percentage of total 1 79.94825760346046 21.27919299789147 2 7.0894636344954565 3.773892501277782 3 2.8609384495695207 2.284419820333014 4 1.694207504574032 1.8037350430884929 5 1.1243111743310301 1.4962448954760244 6 0.8448935617677672 1.3492716691367195 7 0.6302031525659945 1.1741531884899636 8 0.5342908829682804 1.1376636996480365 9 0.44423479206775474 1.064146029461548 >10 4.006346089571089 21.979504378513624 >50 0.523540958097032 9.591542017886448 >100 0.2574289198837988 12.901197035042603 >500 0.025538583321805433 4.845052173195255 >1k 0.013280063327338824 7.607652280142722 >5k 0.002043086665744435 3.6484375311327777 >10k+ 0.0010215433328722174 4.063894739283531 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 15730 2.127731524378284 No Hit GTACATAAGCAGTGGTATCAACGCA 14163 1.9157699669275041 No Hit GCTTATGTACTCTGCGTTGATACCA 8609 1.164503540583131 No Hit GTATCAACGCAGAGTACTTTTTTTT 7141 0.9659333004186477 No Hit CTTATGTACTCTGCGTTGATACCAC 5971 0.8076722779442299 No Hit GGTATCAACGCAGAGTACTTTTTTT 5116 0.6920199922898477 No Hit TATCAACGCAGAGTACTTTTTTTTT 4360 0.5897590239217623 No Hit GAGTACATAAGCAGTGGTATCAACG 4125 0.5579715535957039 No Hit GTGGTATCAACGCAGAGTACATGGG 3784 0.5118459051651257 No Hit TATGTACTCTGCGTTGATACCACTG 3436 0.46477339591632455 No Hit GCGTTGATACCACTGCTTATGTACT 2938 0.39741101199131595 No Hit CCCATGTACTCTGCGTTGATACCAC 2792 0.3776622006398074 No Hit CATAAGCAGTGGTATCAACGCAGAG 2620 0.3543964776777562 No Hit GTACTGGTTCACTATCGGTCAGTCA 2305 0.3117877408577206 No Hit ACGCAGAGTACATAAGCAGTGGTAT 2305 0.3117877408577206 No Hit CTGCTTATGTACTCTGCGTTGATAC 2289 0.3096234875589252 No Hit ACTCTGCGTTGATACCACTGCTTAT 2215 0.29961381605199616 No Hit GTATCAACGCAGAGTACATAAGCAG 1950 0.26376837079069637 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1936 0.2618746491542504 No Hit ATACAGGGTGACAGCCCCGTACACA 1911 0.2584930033748825 No Hit TATCAACGCAGAGTACATAAGCAGT 1771 0.2395557870104222 No Hit GGTATCAACGCAGAGTACATAAGCA 1722 0.23292776128286113 No Hit CATGTACTCTGCGTTGATACCACTG 1692 0.22886978634761965 No Hit GGATACCACGTGTCCCGCCCTACTC 1632 0.2207538364771367 No Hit GGTATCAACGCAGAGTACATGGGGG 1460 0.19748811351508552 No Hit AAGCAGTGGTATCAACGCAGAGTAC 1333 0.18030935295589656 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1307 0.17679244134535396 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1250 0.16908228896839514 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 1249 0.1689470231372204 No Hit GTGGTATCAACGCAGAGTACATAAG 1237 0.16732383316312383 No Hit ACATAAGCAGTGGTATCAACGCAGA 1182 0.15988421244851445 No Hit GTACATGGGAAGCAGTGGTATCAAC 1159 0.15677309833149597 No Hit GTACATGATAAGCAGTGGTATCAAC 976 0.13201945122652292 No Hit GATACCACTGCTTATGTACTCTGCG 971 0.13134312207064933 No Hit ATCATGTACTCTGCGTTGATACCAC 964 0.13039626125242634 No Hit CCCATATTCAGACAGGATACCACGT 957 0.12944940043420333 No Hit CCACTGCTTATGTACTCTGCGTTGA 946 0.12796147629128143 No Hit GTATTTAGCCTTGGAGGATGGTCCC 929 0.12566195716131126 No Hit GTTCACTATCGGTCAGTCAGGAGTA 925 0.1251208938366124 No Hit GGTATCAACGCAGAGTACATGGGCA 917 0.12403876718721468 No Hit GTACTCTGCGTTGATACCACTGCTT 901 0.12187451388841922 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 894 0.1209276530701962 No Hit GCAGTGGTATCAACGCAGAGTACAT 890 0.12038658974549733 No Hit GCCTTGGAGGATGGTCCCCCCATAT 863 0.11673441230378001 No Hit GTTGATACCACTGCTTATGTACTCT 857 0.11592281731673171 No Hit ATACCACTGCTTATGTACTCTGCGT 824 0.11145904488796608 No Hit NNNNNNNNNNNNNNNNNNNNNNNNN 817 0.11051218406974306 No Hit GGTATCAACGCAGAGTACATGGGGA 815 0.11024165240739363 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 771 0.10428995583570612 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 759 0.10266676586160953 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 758 0.1025315000304348 No Hit TATTCAGACAGGATACCACGTGTCC 751 0.1015846392122118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.705316623494322E-4 0.0 0.0 1.352658311747161E-4 0.0 2 2.705316623494322E-4 0.0 0.0 1.352658311747161E-4 0.0 3 2.705316623494322E-4 0.0 0.0 1.352658311747161E-4 0.0 4 2.705316623494322E-4 0.0 0.0 1.352658311747161E-4 0.0 5 6.763291558735806E-4 0.0 0.0 1.352658311747161E-4 0.0 6 0.0013526583117471611 0.0 0.0 1.352658311747161E-4 0.0 7 0.0013526583117471611 0.0 0.0 1.352658311747161E-4 0.0 8 0.0014879241429218773 0.0 0.0 2.705316623494322E-4 0.0 9 0.0014879241429218773 0.0 0.0 2.705316623494322E-4 0.0 10 0.0016231899740965932 0.0 0.0 4.057974935241483E-4 0.0 11 0.0016231899740965932 0.0 0.0 4.057974935241483E-4 0.0 12 0.0016231899740965932 0.0 0.0 4.057974935241483E-4 0.0 13 0.0017584558052713094 0.0 0.0 5.410633246988644E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTATAA 25 0.0060338606 18.997421 10 GTGGTCT 25 0.0060338606 18.997421 9 CCTTACT 60 7.680683E-8 17.414305 13 CCCCTAT 35 0.0021608893 16.29456 1 TGGACTG 35 0.0021608893 16.29456 5 ACGCGCA 35 0.002170711 16.283504 9 ACCCTTA 65 1.9122308E-7 16.074741 11 TTACAGC 50 8.663795E-5 15.208258 4 CTTACTT 70 4.4370063E-7 14.925532 14 GTTACAG 60 2.5515415E-5 14.257742 3 CTCTATA 40 0.005256641 14.25774 1 CTGGACT 40 0.005256641 14.25774 4 ACTATGG 40 0.005280371 14.248066 8 ACGCGTT 40 0.0052827485 14.247099 19 AATCGCC 40 0.005287507 14.245166 18 AACGGTT 55 1.9602067E-4 13.816307 7 TTAGCAC 50 0.0014921218 13.307226 3 AGGTTTA 50 0.0014997752 13.298195 9 AACGCGT 50 0.0015020778 13.295488 18 TCGAACG 50 0.0015020778 13.295488 18 >>END_MODULE