FastQCFastQC Report
Wed 25 May 2016
SRR1295164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739285
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT227343.075133405925996No Hit
GGTATCAACGCAGAGTACTTTTTTT168062.273277558722279No Hit
GTACATAAGCAGTGGTATCAACGCA155342.10121942146804No Hit
TATCAACGCAGAGTACTTTTTTTTT144621.9562144504487444No Hit
GCTTATGTACTCTGCGTTGATACCA91251.2343007094692846No Hit
ACGCAGAGTACTTTTTTTTTTTTTT79531.0757691553325173No Hit
CTTATGTACTCTGCGTTGATACCAC68410.9253535510662328No Hit
GTACTTTTTTTTTTTTTTTTTTTTT64200.8684066361416773No Hit
GAGTACATAAGCAGTGGTATCAACG45340.6132952785461628No Hit
GTGGTATCAACGCAGAGTACATGGG42770.5785319599342609No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40380.5462034262835037No Hit
CATAAGCAGTGGTATCAACGCAGAG33510.4532758002664737No Hit
TATGTACTCTGCGTTGATACCACTG33090.4475946353571356No Hit
CCCATGTACTCTGCGTTGATACCAC32120.43447384973318814No Hit
GCGTTGATACCACTGCTTATGTACT31610.4275752923432776No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31090.42054146912219237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28180.38117911225035No Hit
CTGCTTATGTACTCTGCGTTGATAC26570.3594013134312207No Hit
ACGCAGAGTACATAAGCAGTGGTAT26400.35710179430125055No Hit
GTACTGGTTCACTATCGGTCAGTCA24910.3369471854562178No Hit
ACTCTGCGTTGATACCACTGCTTAT23340.3157104499617874No Hit
GTGGTATCAACGCAGAGTACTTTTT22590.3055655126236837No Hit
ATCAACGCAGAGTACTTTTTTTTTT22580.30543024679250896No Hit
GTATCAACGCAGAGTACATAAGCAG21280.2878456887397959No Hit
ATACAGGGTGACAGCCCCGTACACA20400.27594229559642086No Hit
CATGTACTCTGCGTTGATACCACTG20040.2710727256741311No Hit
GGTATCAACGCAGAGTACATAAGCA19310.26119831999837684No Hit
TATCAACGCAGAGTACATAAGCAGT18390.24875386353030293No Hit
GGATACCACGTGTCCCGCCCTACTC18090.24469588859506144No Hit
GGTATCAACGCAGAGTACATGGGGG15460.20912097499611113No Hit
AAGCAGTGGTATCAACGCAGAGTAC15420.20857991167141224No Hit
GTGGTATCAACGCAGAGTACATAAG15060.20371034174912245No Hit
ACATAAGCAGTGGTATCAACGCAGA14280.1931596069174946No Hit
ACCCTGTATCGCGCGCCTTTCCAGA13410.1813914796052943No Hit
CCCATATTCAGACAGGATACCACGT11200.15149773091568203No Hit
GTACATGGGAAGCAGTGGTATCAAC11190.15136246508450732No Hit
GATACCACTGCTTATGTACTCTGCG10850.14676342682456697No Hit
CCACTGCTTATGTACTCTGCGTTGA10590.14324651521402437No Hit
GGTATCAACGCAGAGTACATGGGCA10400.14067646442170476No Hit
CAACGCAGAGTACTTTTTTTTTTTT10180.137700616135861No Hit
GTACATGATAAGCAGTGGTATCAAC10140.13715955281116216No Hit
ATCATGTACTCTGCGTTGATACCAC9900.13391317286296894No Hit
TCTAAGTACCCCGAGGAAAAGAAAT9880.13364264120061953No Hit
GCCTTGGAGGATGGTCCCCCCATAT9630.13026099542125164No Hit
GTACTCTGCGTTGATACCACTGCTT9450.12782621046010673No Hit
GTTCACTATCGGTCAGTCAGGAGTA9210.12457983051191354No Hit
GGTATCAACGCAGAGTACATGGGGA9200.12444456468073883No Hit
GCAGTGGTATCAACGCAGAGTACAT9180.12417403301838939No Hit
AACGCAGAGTACTTTTTTTTTTTTT9170.12403876718721468No Hit
ATACCACTGCTTATGTACTCTGCGT9040.12228031138194337No Hit
CTATCGGTCAGTCAGGAGTATTTAG8930.12079238723902148No Hit
GTATTTAGCCTTGGAGGATGGTCCC8720.11795180478435245No Hit
GTCAGGAGTATTTAGCCTTGGAGGA8610.11646388064143057No Hit
GTATCAACGCAGAGTACATGGGGGT8220.11118851322561665No Hit
GTTGATACCACTGCTTATGTACTCT7950.1075363357838993No Hit
GCCCAGAGCCTGAATCAGTGTGTGT7540.10199043670573595No Hit
GTTAATGATAGTGTGTCGAAACACA7510.1015846392122118No Hit
TCAACGCAGAGTACTTTTTTTTTTT7480.10117884171868764No Hit
TATTCAGACAGGATACCACGTGTCC7440.1006377783939888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTT307.714351E-419.001156
TAACGTT250.00602704319.001155
ACTCCTA359.926051E-519.001157
ATCGTAA359.931207E-518.99986512
TGAGGTT359.936365E-518.9985816
CTTATAC659.986252E-917.5407091
ACGCACA453.535054E-516.888774
GTCCAGA402.7559642E-416.627131
AGGGAAT402.757387E-416.6260056
GATCGTA402.7588106E-416.62488211
TCGTAAA402.7588106E-416.62488213
GTCTCGG350.002166895216.2878041
GCGTATC350.002166895216.2878041
CGTAAAA456.7532965E-414.77867214
GTTCTCG456.756762E-414.77767313
TTATGCC456.756762E-414.7776734
GGATCGT456.756762E-414.77767310
TCCTACG456.756762E-414.7776739
TTATGCT653.3665947E-614.615284
TGTAAGA851.711851E-814.5293082
Produced by FastQC (version 0.11.5)