FastQCFastQC Report
Wed 25 May 2016
SRR1295163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438518
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31800.027.1770041
GTACATG62650.025.421391
TCAACGC32000.025.2123764
ATCAACG32400.024.5566843
CAACGCA31400.023.0463925
TACATGG66550.022.438442
GGTATCA23700.021.6187421
TATCAAC37100.021.4556262
AACGCAG33200.021.2492646
ACATGGG69000.020.8694233
ACGCAGA37400.018.9386967
CATGGGG51000.018.895554
CTATTAG10350.018.4893671
CGCAGAG38150.018.1149258
GTATAGA12500.017.2845751
TAGACCG2203.917694E-415.8796155
GTATAGC6703.6379788E-1215.6629941
CCTATAC9950.015.2043243
GTATTAA18700.015.1850731
TAGTGCG2305.5148476E-415.1811127
Produced by FastQC (version 0.11.5)