##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295163_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3438518 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.67954479226225 33.0 27.0 33.0 15.0 33.0 2 22.901543339310713 33.0 6.0 33.0 2.0 33.0 3 25.191322249876254 33.0 15.0 33.0 15.0 33.0 4 25.48022607414008 33.0 15.0 37.0 2.0 37.0 5 27.215119129811157 33.0 15.0 37.0 2.0 37.0 6 28.11406803745102 33.0 15.0 37.0 6.0 37.0 7 28.00165274691015 33.0 15.0 37.0 6.0 37.0 8 28.670891645761344 33.0 27.0 37.0 6.0 37.0 9 29.734797084092623 37.0 27.0 37.0 15.0 37.0 10-11 29.243542857707887 35.0 24.5 37.0 10.5 37.0 12-13 28.04440139618289 33.0 18.5 37.0 4.0 37.0 14-15 29.908924571574147 35.0 27.0 37.0 10.5 40.0 16-17 30.341119051870603 37.0 27.0 40.0 10.5 40.0 18-19 30.463151567041383 37.0 27.0 40.0 10.5 40.0 20-21 29.989443417193105 35.0 27.0 40.0 6.0 40.0 22-23 30.715606258277546 37.0 27.0 40.0 15.0 40.0 24-25 30.839348085425172 37.0 27.0 40.0 15.0 40.0 26-27 29.43066126162492 35.0 24.5 40.0 4.0 40.0 28-29 30.11073884155907 35.0 27.0 40.0 10.5 40.0 30-31 30.776003353770435 37.0 27.0 40.0 15.0 40.0 32-33 29.579325453582037 33.0 27.0 40.0 6.0 40.0 34-35 29.213662688402387 33.0 22.0 37.0 6.0 40.0 36-37 29.52566759865733 33.0 27.0 37.0 6.0 40.0 38-39 29.65523039286111 33.0 24.5 37.0 6.0 40.0 40-41 28.732863693021237 33.0 22.0 37.0 6.0 40.0 42-43 29.37698014086301 33.0 22.0 37.0 6.0 40.0 44-45 29.291784861966697 33.0 22.0 37.0 6.0 40.0 46-47 28.95633351344969 33.0 22.0 37.0 6.0 40.0 48-49 28.874864112969597 33.0 22.0 37.0 6.0 40.0 50-51 28.812084450335874 33.0 22.0 37.0 6.0 40.0 52-53 29.004366125173696 33.0 22.0 37.0 6.0 40.0 54-55 28.99998080568431 33.0 22.0 37.0 6.0 40.0 56-57 28.983762772217567 33.0 22.0 37.0 6.0 40.0 58-59 28.826231097234334 33.0 22.0 37.0 10.5 40.0 60-61 28.760853949288617 33.0 22.0 37.0 10.5 40.0 62-63 28.688048164936173 33.0 22.0 37.0 10.5 40.0 64-65 28.27447144380224 33.0 22.0 37.0 6.0 40.0 66-67 28.472314526199952 33.0 22.0 37.0 10.5 40.0 68-69 28.036486940013113 33.0 22.0 37.0 10.5 38.5 70-71 28.169859660470003 33.0 22.0 37.0 10.5 37.0 72-73 28.189930661988683 33.0 22.0 37.0 10.5 37.0 74-75 28.275548506653156 33.0 22.0 37.0 15.0 37.0 76-77 28.051570327681866 33.0 22.0 37.0 15.0 37.0 78-79 27.591955458717973 33.0 22.0 37.0 10.5 37.0 80-81 27.405344977109323 33.0 22.0 33.0 10.5 37.0 82-83 27.484103325909594 33.0 22.0 33.0 15.0 37.0 84-85 27.447022816224894 30.0 22.0 33.0 15.0 37.0 86-87 27.241505788249473 27.0 22.0 33.0 15.0 37.0 88-89 27.186962668219273 27.0 22.0 33.0 15.0 37.0 90-91 27.335132751958838 27.0 22.0 33.0 15.0 37.0 92-93 27.27836759906448 27.0 22.0 33.0 15.0 37.0 94-95 27.14891793499409 27.0 22.0 33.0 15.0 37.0 96-97 27.2402929110739 27.0 22.0 33.0 15.0 37.0 98-99 27.31258931900313 27.0 22.0 33.0 15.0 37.0 100 27.010192181631737 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.428826920790954 1101 2 11.355950207522689 1101 3 8.493519103467275 1101 4 13.118352082192065 1101 5 11.024444750454837 1101 6 10.095225771832915 1101 7 9.68049427532851 1101 8 9.102171592562069 1101 9 7.547528602783448 1101 10-11 7.910745328090844 1101 12-13 9.37856320767029 1101 14-15 8.287668651611561 1101 16-17 8.38323015209362 1101 18-19 8.394212540533449 1101 20-21 7.378625104879525 1101 22-23 7.566581737310273 1101 24-25 7.529153299001003 1101 26-27 8.76719940172536 1101 28-29 8.37775679770402 1101 30-31 5.7767053615899435 1101 32-33 7.148503797604633 1101 34-35 8.99433310582037 1101 36-37 8.390143859309859 1101 38-39 8.346082463761135 1101 40-41 9.260524915797763 1101 42-43 8.78987266281905 1101 44-45 8.724504192119152 1101 46-47 7.466080739780697 1101 48-49 5.654683267698655 1101 50-51 7.02726165802348 1101 52-53 6.043382646865513 1101 54-55 4.049312427460631 1101 56-57 6.33154023058394 1101 58-59 6.3430179207317146 1101 60-61 6.879094808674335 1101 62-63 6.396147698947882 1101 64-65 6.739187321408213 1101 66-67 5.85501731200036 1101 68-69 6.905311536711341 1101 70-71 6.311688979710148 1101 72-73 5.612811801788098 1101 74-75 4.974304436072462 1101 76-77 5.216295942718599 1101 78-79 5.228373101536452 1101 80-81 5.069642107518998 1101 82-83 4.629783043614793 1101 84-85 4.451586381654625 1101 86-87 4.301940979548032 1101 88-89 4.222661048763541 1101 90-91 3.9502790146304356 1101 92-93 3.937528670096924 1101 94-95 3.802933203761434 1101 96-97 3.9120826098536234 1101 98-99 3.716725740463634 1101 100 3.928672947743866 1102 1 6.866452123064718 1102 2 10.703946901989031 1102 3 8.31592965351361 1102 4 12.829231229668661 1102 5 10.801800876756655 1102 6 9.717389098389212 1102 7 6.858578379722516 1102 8 8.001503363028025 1102 9 6.620317076290771 1102 10-11 7.61218966650004 1102 12-13 9.212983217904998 1102 14-15 6.30728924135196 1102 16-17 7.428483322778096 1102 18-19 7.586906481310454 1102 20-21 7.082107809608239 1102 22-23 6.761095242973937 1102 24-25 7.100601821192335 1102 26-27 8.686188551553169 1102 28-29 7.822380914269036 1102 30-31 4.329006303459575 1102 32-33 4.668583289465271 1102 34-35 5.326981439914391 1102 36-37 5.385864991213104 1102 38-39 4.72441583167118 1102 40-41 6.5318744794396615 1102 42-43 7.302677041613848 1102 44-45 6.640039197582272 1102 46-47 5.602171047236819 1102 48-49 3.8891102970058746 1102 50-51 5.107471953585218 1102 52-53 6.044682558349134 1102 54-55 5.600244926803672 1102 56-57 6.550791921385148 1102 58-59 6.301528943787471 1102 60-61 6.375824059189522 1102 62-63 4.449425467505204 1102 64-65 6.279044208397696 1102 66-67 3.944379648279625 1102 68-69 6.326855974701466 1102 70-71 5.529439211374118 1102 72-73 5.226384475121154 1102 74-75 4.567524911018175 1102 76-77 3.5525277380797853 1102 78-79 4.546678635193423 1102 80-81 4.7074323444386295 1102 82-83 4.097587699023279 1102 84-85 3.17741736377155 1102 86-87 3.5425594186169533 1102 88-89 3.5973270723033686 1102 90-91 3.5222467407275566 1102 92-93 3.4623994223478682 1102 94-95 3.306480359481114 1102 96-97 3.4758475297048044 1102 98-99 3.325579799078074 1102 100 3.502274942128608 1103 1 6.788319394844095 1103 2 11.087637940763937 1103 3 8.111352275828725 1103 4 12.294865501287479 1103 5 10.529391489503343 1103 6 9.252150396674942 1103 7 8.254602266136455 1103 8 8.213308030695405 1103 9 7.26734321926428 1103 10-11 7.620544230093149 1103 12-13 8.828289006233987 1103 14-15 8.797151046662854 1103 16-17 8.230305208229279 1103 18-19 7.419906501721403 1103 20-21 8.65680285640321 1103 22-23 7.073476361784188 1103 24-25 5.4210805969696025 1103 26-27 6.157782038146845 1103 28-29 7.348682944037105 1103 30-31 6.398872005177157 1103 32-33 8.228869761779478 1103 34-35 7.49188646756188 1103 36-37 4.517684205992467 1103 38-39 6.720877290340951 1103 40-41 7.164416944271384 1103 42-43 7.8611213810329446 1103 44-45 5.863163325535336 1103 46-47 6.608285464466189 1103 48-49 5.9290714547579135 1103 50-51 6.695243859382661 1103 52-53 6.3916742560526565 1103 54-55 5.229059698680437 1103 56-57 6.209326119280213 1103 58-59 5.815467994615187 1103 60-61 5.239195353291006 1103 62-63 2.9898565262253456 1103 64-65 5.318091023995279 1103 66-67 4.466510774672116 1103 68-69 5.490191964692055 1103 70-71 3.868070425231828 1103 72-73 4.587760653044818 1103 74-75 3.585532523007796 1103 76-77 3.8062390357977414 1103 78-79 3.180633111738153 1103 80-81 4.372130280029442 1103 82-83 3.7338727607541777 1103 84-85 3.645169028593326 1103 86-87 3.0428691942675847 1103 88-89 3.3551457856490075 1103 90-91 2.997827836438944 1103 92-93 2.5426859726405127 1103 94-95 3.110620055309134 1103 96-97 3.3228126626325327 1103 98-99 3.032357965574196 1103 100 3.167153297196009 1104 1 6.578287012653334 1104 2 9.96673017112439 1104 3 7.5902036391413255 1104 4 12.501374503050524 1104 5 9.685177668632942 1104 6 8.462766905627625 1104 7 4.243925000942262 1104 8 6.76604640221381 1104 9 6.857015683862517 1104 10-11 7.440218109837222 1104 12-13 7.68030537398824 1104 14-15 7.558743639540694 1104 16-17 7.530281642071703 1104 18-19 7.166404052548604 1104 20-21 7.832954645691437 1104 22-23 6.789421485741059 1104 24-25 6.19300235747351 1104 26-27 8.561830776070753 1104 28-29 7.554013343827112 1104 30-31 6.615514307648649 1104 32-33 7.952564808090813 1104 34-35 8.202859593631889 1104 36-37 6.954031235041853 1104 38-39 7.576076706886937 1104 40-41 7.369968356056265 1104 42-43 7.5258066382811535 1104 44-45 7.07806868768078 1104 46-47 6.928658581386184 1104 48-49 5.73954999296576 1104 50-51 5.987958549311411 1104 52-53 6.2665008299217355 1104 54-55 5.0204062335592035 1104 56-57 6.369548042600336 1104 58-59 4.153722735459407 1104 60-61 4.525625703899227 1104 62-63 4.141137113727577 1104 64-65 3.0117859620430814 1104 66-67 3.4722859537494557 1104 68-69 4.310881343453694 1104 70-71 2.723094028426921 1104 72-73 2.605806923920497 1104 74-75 3.408515682404772 1104 76-77 4.024829796853023 1104 78-79 4.516252343362307 1104 80-81 3.1821857173886308 1104 82-83 3.2556450891002875 1104 84-85 3.2844543573395697 1104 86-87 0.9500221452841089 1104 88-89 0.5055961260789807 1104 90-91 2.4677659223842667 1104 92-93 2.6895783030188163 1104 94-95 2.6562616055788766 1104 96-97 3.053687106405686 1104 98-99 2.6961616286936128 1104 100 0.9406518268247481 1105 1 5.873286527796893 1105 2 -2.8146065980139703 1105 3 0.37766852911549265 1105 4 8.780837963219447 1105 5 8.749681206182832 1105 6 8.303779479741983 1105 7 5.38550011826344 1105 8 5.969241578713444 1105 9 4.993438373914813 1105 10-11 5.0416570177978635 1105 12-13 5.3191134900497445 1105 14-15 6.110075103607052 1105 16-17 6.257560237801631 1105 18-19 6.3814342162523054 1105 20-21 7.209431569432972 1105 22-23 2.134727782777027 1105 24-25 4.137476638299361 1105 26-27 6.971483302085009 1105 28-29 6.081984082640414 1105 30-31 5.3967196113745395 1105 32-33 7.325125569601099 1105 34-35 6.3722798461603425 1105 36-37 6.449576536490326 1105 38-39 4.279406814672111 1105 40-41 5.642352080038307 1105 42-43 5.8552457385748795 1105 44-45 6.418554357639902 1105 46-47 5.6320657888439065 1105 48-49 5.207295425886958 1105 50-51 6.105606659785085 1105 52-53 5.75438576542302 1105 54-55 5.471767332212071 1105 56-57 5.332627312988507 1105 58-59 2.0370761617117914 1105 60-61 4.597187953777954 1105 62-63 -0.8319088188024288 1105 64-65 -0.36845447005307363 1105 66-67 0.8784981663595346 1105 68-69 2.860168079577555 1105 70-71 3.1019061275904676 1105 72-73 2.823169720796301 1105 74-75 2.779044765579105 1105 76-77 1.7965335147087913 1105 78-79 3.337856586088428 1105 80-81 1.6031974549381651 1105 82-83 0.9624654042302723 1105 84-85 1.380667506009722 1105 86-87 -0.19378174789910574 1105 88-89 1.7839696743308302 1105 90-91 1.4763653296047465 1105 92-93 1.7596504926740266 1105 94-95 1.11940980934763 1105 96-97 2.0525210915975016 1105 98-99 1.9238423480032871 1105 100 2.1571121341282584 1106 1 6.173439743728185 1106 2 10.553105022728897 1106 3 6.931594411456931 1106 4 12.006812481535764 1106 5 10.07006031262286 1106 6 9.567030813686138 1106 7 6.841651287481234 1106 8 7.579785819876509 1106 9 7.015559207082784 1106 10-11 5.829170548480736 1106 12-13 8.082939824094552 1106 14-15 8.003516248875108 1106 16-17 7.845825781838602 1106 18-19 7.3234788577280305 1106 20-21 8.298096203908976 1106 22-23 6.338089183290414 1106 24-25 5.137569724652444 1106 26-27 7.834045983944005 1106 28-29 6.341604617443171 1106 30-31 5.467717556033616 1106 32-33 7.469161108708946 1106 34-35 7.00042021196904 1106 36-37 6.190541534439873 1106 38-39 7.286097654673 1106 40-41 6.708912489145188 1106 42-43 6.316494405637258 1106 44-45 4.639826190784294 1106 46-47 6.23735218781874 1106 48-49 6.052202525742533 1106 50-51 6.670019486383563 1106 52-53 5.769169132863457 1106 54-55 5.461734352430735 1106 56-57 5.023637723562054 1106 58-59 5.146547078939673 1106 60-61 4.972323763873309 1106 62-63 5.07466149243491 1106 64-65 5.307899396271257 1106 66-67 3.1008970291782525 1106 68-69 3.3485950679552587 1106 70-71 3.0340372791622983 1106 72-73 2.8598048355209613 1106 74-75 2.8947507429775463 1106 76-77 3.498655261665178 1106 78-79 4.280621599826169 1106 80-81 3.756209760957635 1106 82-83 2.1386301681176967 1106 84-85 2.7883264189192865 1106 86-87 3.4090471282173525 1106 88-89 3.4409668275671805 1106 90-91 3.1628781900802565 1106 92-93 2.996989688200877 1106 94-95 1.5026955372523574 1106 96-97 1.5360023170201167 1106 98-99 2.010035134495727 1106 100 3.0084408083629057 1107 1 6.108196668690113 1107 2 10.409017434413446 1107 3 6.270849126385535 1107 4 11.614272632230264 1107 5 9.52970831042428 1107 6 8.094022563276766 1107 7 3.8184201729602876 1107 8 6.235292295388657 1107 9 5.7405576325771825 1107 10-11 4.648275885768459 1107 12-13 6.184166721803216 1107 14-15 6.220065519449605 1107 16-17 7.005342451558857 1107 18-19 6.321924534421914 1107 20-21 5.443965059043009 1107 22-23 5.760645908433261 1107 24-25 4.574208684791685 1107 26-27 7.247369378807104 1107 28-29 6.472970701447341 1107 30-31 5.11547159535618 1107 32-33 4.971448793784919 1107 34-35 6.654420959143518 1107 36-37 6.463864256559674 1107 38-39 6.482799423272212 1107 40-41 7.578821325270649 1107 42-43 6.178077399259081 1107 44-45 4.144668439636337 1107 46-47 3.527205644287953 1107 48-49 4.034979295200561 1107 50-51 4.784221169100643 1107 52-53 3.8679433802497734 1107 54-55 5.167335727689814 1107 56-57 5.116110513242461 1107 58-59 2.8958578748951957 1107 60-61 2.8511498124940466 1107 62-63 0.2979162245399394 1107 64-65 2.0813416376801825 1107 66-67 4.099847334918614 1107 68-69 5.038185868262143 1107 70-71 4.25260571003864 1107 72-73 2.244236553506969 1107 74-75 2.436124527745495 1107 76-77 3.029141626446634 1107 78-79 3.827015194737367 1107 80-81 2.118471366495317 1107 82-83 1.668621851865364 1107 84-85 2.3683625466660843 1107 86-87 2.26246809948692 1107 88-89 2.548736297044673 1107 90-91 1.3800994882744426 1107 92-93 2.1722612904177794 1107 94-95 2.2527498576957363 1107 96-97 2.3065134731080157 1107 98-99 1.7016092393177225 1107 100 -0.02932170466255002 1108 1 4.375533066697308 1108 2 -3.2842876106179943 1108 3 -0.047162796129761375 1108 4 6.975141014521451 1108 5 7.219023251009318 1108 6 6.925472811318443 1108 7 4.426501535852065 1108 8 5.045764866122351 1108 9 4.9319140207177625 1108 10-11 3.27774570859987 1108 12-13 2.5293583515524105 1108 14-15 4.070640392817431 1108 16-17 4.737139830906294 1108 18-19 4.119477182846396 1108 20-21 4.81917807704923 1108 22-23 4.22256773560655 1108 24-25 2.996587794029537 1108 26-27 4.800180244295756 1108 28-29 6.207043154741466 1108 30-31 4.596215353379858 1108 32-33 4.655122700675953 1108 34-35 3.0431430381343283 1108 36-37 1.0724648872327123 1108 38-39 2.500588367650039 1108 40-41 5.536827559307088 1108 42-43 3.3121520404296945 1108 44-45 3.8551258187438115 1108 46-47 4.7981183998901535 1108 48-49 4.876842467875537 1108 50-51 2.3925597662765874 1108 52-53 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-24.113907173145346 2213 52-53 -18.433990485782186 2213 54-55 -18.323197988424063 2213 56-57 -18.200539474393164 2213 58-59 -18.64272864484144 2213 60-61 -19.953030313313505 2213 62-63 -18.100155318843573 2213 64-65 -18.2801077765574 2213 66-67 -8.988045427283481 2213 68-69 -23.44650664510684 2213 70-71 -18.091112799435564 2213 72-73 -9.893251275308216 2213 74-75 -4.545760498992475 2213 76-77 -3.335827771717252 2213 78-79 -4.992920626411902 2213 80-81 -3.671168830098299 2213 82-83 -3.6101498630738966 2213 84-85 -3.281615236091792 2213 86-87 -3.425220457281032 2213 88-89 -2.9766601358322085 2213 90-91 -3.007787478125362 2213 92-93 -3.991656169629824 2213 94-95 -3.9356766978975983 2213 96-97 -3.6399323477321452 2213 98-99 -3.929773677748212 2213 100 -3.6510262114784133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 899.0 3 745.0 4 4220.0 5 15573.0 6 24469.0 7 28377.0 8 25178.0 9 24442.0 10 28633.0 11 35710.0 12 45078.0 13 60658.0 14 46008.0 15 50921.0 16 59468.0 17 63869.0 18 64444.0 19 65287.0 20 64659.0 21 62983.0 22 66099.0 23 73118.0 24 82219.0 25 91431.0 26 101807.0 27 110131.0 28 119933.0 29 125923.0 30 133193.0 31 145293.0 32 166241.0 33 204957.0 34 248615.0 35 296579.0 36 326936.0 37 285673.0 38 88581.0 39 168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.41067914552517 20.190768780538658 14.156906175885714 24.241645898050457 2 15.317658079051771 20.39392724200421 40.33028542391144 23.95812925503257 3 18.151242906425196 25.402789030066288 29.52468377165523 26.921284291853286 4 12.467043630571304 16.863285994153294 38.88702831196255 31.78264206331285 5 13.6119400613265 37.06608125529291 35.44528586477845 13.876692818602152 6 31.214950392805825 37.295075787833206 18.369113967744656 13.120859851616313 7 26.769843141656818 32.030310979831775 23.28353813497864 17.916307743532762 8 24.19479934246019 36.2330140837919 21.18198493243715 18.390201641310764 9 24.45907857117894 17.18425326815319 21.72410600582354 36.63256215484434 10-11 23.725181889935953 26.970450945005354 28.82580927705642 20.47855788800227 12-13 24.34675360021322 24.477534208652436 28.869384237004887 22.30632795412946 14-15 21.620271062750458 26.14158421452575 26.047153409042878 26.19099131368091 16-17 21.592666309965196 29.64036724423899 26.764151093209303 22.002815352586513 18-19 21.539681161287795 28.825306651701748 28.22722339408008 21.407788792930383 20-21 22.840353720516735 28.068714301449184 27.46517080232247 21.62576117571161 22-23 22.163510729252806 27.90944181332004 27.972406026143393 21.954641431283754 24-25 22.238214628171452 27.809177501653704 27.635138087160744 22.3174697830141 26-27 22.112147660829976 27.89581263015718 27.718214686480085 22.27382502253276 28-29 21.926171537427816 27.857658322589124 28.01648984807514 22.199680291907928 30-31 22.411139760696916 28.162245906172263 27.362225498340575 22.064388834790254 32-33 21.88568856814143 27.951561028738343 27.875618789353208 22.287131613767023 34-35 22.05918706850185 27.4892468262394 27.683746214160905 22.767819891097844 36-37 22.345687779016767 28.066974168733893 27.389881772192005 22.19745628005734 38-39 22.055916800123487 27.864086287070442 27.64415459123623 22.435842321569837 40-41 22.42431691085437 27.724995870240505 27.374975985626694 22.475711233278435 42-43 21.986307081339447 27.9384736233381 27.812539814458038 22.262679480864417 44-45 22.299794851945034 27.572177278885235 27.736682794658407 22.39134507451132 46-47 22.277875169149375 27.869245450321458 27.646073173726666 22.206806206802504 48-49 22.08886248959848 27.697773186947224 27.73001846623984 22.483345857214456 50-51 22.461698102923037 27.674626011327398 27.518059431630082 22.345616454119487 52-53 22.027802009370294 27.679283257613722 27.743564745168513 22.54934998784747 54-55 22.29780861188382 27.722052335542124 27.605618198884464 22.374520853689596 56-57 22.36638222698905 27.72746472679203 27.52221024248077 22.383942803738147 58-59 22.117758705746336 27.354680786162692 27.815502573971067 22.712057934119898 60-61 22.40800755120624 27.659247273217613 27.516419782827068 22.416325392749084 62-63 22.133824612674946 27.791808377052895 27.709565094762805 22.364801915509354 64-65 22.201300710976696 27.368620104472775 27.70480330333891 22.72527588121162 66-67 22.159329954771444 27.57973261708178 27.52899912447892 22.731938303667857 68-69 22.46951003115692 27.572556702898112 27.541626180622014 22.416307085322963 70-71 22.62374343901028 27.49977506880511 27.371951586465045 22.50452990571957 72-73 22.321854270410743 27.692800637876108 27.65482338436096 22.330521707352187 74-75 22.423051403323196 27.42852645311603 27.394493350133942 22.753928793426834 76-77 22.557544975088074 27.63280406822754 27.344009774847468 22.46564118183692 78-79 22.472616449241105 27.448234098962406 27.53599221847438 22.543157233322113 80-81 22.64303920083321 27.386096222722543 27.401186992142563 22.56967758430168 82-83 22.291875564399476 27.500012092311387 27.80481162597323 22.40330071731591 84-85 22.42575897451754 27.203010131439264 27.485103206399547 22.886127687643647 86-87 22.4727680841859 27.553171942355885 27.522455796062317 22.451604177395897 88-89 22.35030395548597 27.204481049269308 27.66682617683423 22.77838881841049 90-91 22.511354911202428 27.24757069164404 27.434527537510633 22.806546859642896 92-93 22.290226371375123 27.443990813238507 27.67898572083101 22.586797094555354 94-95 22.403502308459284 26.98849132902343 27.599684798086564 23.008321564430723 96-97 22.41081411604111 27.223979472702037 27.59672322376483 22.768483187492023 98-99 22.280846408851403 27.196502962407603 27.64279248771531 22.87985814102568 100 22.585839182237212 26.81299953757948 27.47639625144342 23.124765028739887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 365.0 1 459.0 2 444.5 3 345.5 4 372.0 5 491.5 6 720.0 7 1044.0 8 1529.0 9 2207.5 10 3092.5 11 4160.0 12 5307.5 13 6431.0 14 7493.0 15 8368.0 16 9140.5 17 10060.0 18 10866.0 19 11549.0 20 12457.5 21 13563.0 22 14710.5 23 16045.0 24 17853.5 25 20255.5 26 23374.5 27 27620.5 28 33753.5 29 41422.0 30 50204.5 31 60311.0 32 70932.0 33 81176.0 34 91249.5 35 101098.0 36 109925.0 37 117845.5 38 124123.5 39 128462.5 40 131740.0 41 133012.0 42 132063.5 43 130052.0 44 126518.5 45 122305.0 46 117967.0 47 113388.5 48 109054.0 49 104464.5 50 99898.5 51 94910.5 52 88910.5 53 82920.5 54 77592.0 55 72532.0 56 67596.5 57 62260.5 58 56411.0 59 50327.5 60 44053.5 61 37544.5 62 30900.5 63 24928.0 64 20115.5 65 16232.5 66 12732.5 67 9621.5 68 7265.0 69 5522.0 70 4228.5 71 3131.5 72 2239.5 73 1620.5 74 1179.5 75 850.0 76 567.5 77 367.0 78 251.5 79 187.0 80 135.0 81 89.5 82 53.0 83 39.0 84 27.5 85 19.0 86 13.5 87 8.5 88 5.0 89 3.0 90 2.5 91 3.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.381813909364442 2 23.99702429942202 3 5.842778778531914 4 23.75645554276581 5 12.155352974740863 6 9.344985252367444 7 7.764798671986013 8 8.357612203862246 9 5.135468245331273 10-11 6.632508540016367 12-13 9.760353152142871 14-15 7.114838427485329 16-17 7.516450401015787 18-19 6.563030933675497 20-21 7.5866405236209316 22-23 5.590475315237553 24-25 5.1446727921738375 26-27 9.395152213831656 28-29 6.142151938713131 30-31 4.236258178668834 32-33 8.531713371865438 34-35 6.943834524059493 36-37 6.826938233273753 38-39 5.796988702691102 40-41 8.188251450188714 42-43 6.1833906351515395 44-45 6.082358737107091 46-47 5.737937099645836 48-49 3.7663464318057955 50-51 6.363250097861927 52-53 4.335574221219724 54-55 4.710968504454535 56-57 4.607900845655018 58-59 3.4674967529615954 60-61 4.0244954366968555 62-63 3.0746676329744385 64-65 4.3402419298081325 66-67 2.2918158346124695 68-69 4.941140340111641 70-71 2.382887046105328 72-73 1.5204370022201426 74-75 0.7891044921096821 76-77 1.0799710805643594 78-79 1.6927059855437723 80-81 2.004017428438647 82-83 0.7927688614688072 84-85 1.2404471926568363 86-87 0.44312695178562395 88-89 0.7253851804760074 90-91 0.32736487056342295 92-93 0.34456704894376006 94-95 0.5376734977103508 96-97 0.5489719699009864 98-99 0.46426978134184554 100 0.442836128820614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3438518.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.48439500672093 #Duplication Level Percentage of deduplicated Percentage of total 1 94.7584358625408 86.68918176667711 2 3.8176107805482333 6.985036252591816 3 0.7539502701882628 2.0692405299998096 4 0.26083420623890785 0.9544903821929903 5 0.13095734520381952 0.5990276748828867 6 0.07998281894684436 0.43903078813704915 7 0.0475554842932256 0.3045409296872154 8 0.03320079532614986 0.24298837393238307 9 0.023435345776786444 0.1929571587146358 >10 0.09241083030709452 1.386825937066916 >50 0.0013363916232068918 0.07592521576127315 >100 2.8986900665869367E-4 0.06075499035586263 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.908229650099258E-5 0.0 0.0 0.0 0.0 6 2.908229650099258E-5 0.0 0.0 0.0 0.0 7 2.908229650099258E-5 0.0 0.0 0.0 0.0 8 2.908229650099258E-5 0.0 0.0 2.908229650099258E-5 0.0 9 2.908229650099258E-5 0.0 0.0 2.908229650099258E-5 0.0 10-11 2.908229650099258E-5 0.0 0.0 2.908229650099258E-5 2.908229650099258E-5 12-13 2.908229650099258E-5 0.0 0.0 2.908229650099258E-5 2.908229650099258E-5 14-15 2.908229650099258E-5 0.0 0.0 2.908229650099258E-5 4.3623444751488864E-5 16-17 2.908229650099258E-5 0.0 0.0 4.3623444751488864E-5 5.816459300198516E-5 18-19 2.908229650099258E-5 0.0 0.0 7.270574125248145E-5 5.816459300198516E-5 20-21 2.908229650099258E-5 0.0 0.0 8.724688950297774E-5 5.816459300198516E-5 22-23 2.908229650099258E-5 0.0 0.0 8.724688950297774E-5 5.816459300198516E-5 24-25 2.908229650099258E-5 0.0 0.0 1.454114825049629E-4 5.816459300198516E-5 26-27 2.908229650099258E-5 0.0 0.0 2.4719952025843695E-4 5.816459300198516E-5 28-29 2.908229650099258E-5 0.0 0.0 6.398105230218368E-4 5.816459300198516E-5 30-31 2.908229650099258E-5 0.0 0.0 0.0019485138655665028 8.724688950297774E-5 32-33 2.908229650099258E-5 0.0 0.0 0.006150905709959931 8.724688950297774E-5 34-35 2.908229650099258E-5 0.0 0.0 0.012636257829681277 8.724688950297774E-5 36-37 2.908229650099258E-5 0.0 0.0 0.02259694438127123 8.724688950297774E-5 38-39 2.908229650099258E-5 0.0 0.0 0.037806985451290354 8.724688950297774E-5 40-41 2.908229650099258E-5 0.0 0.0 0.05663777243568305 8.724688950297774E-5 42-43 2.908229650099258E-5 0.0 0.0 0.07702446228287885 8.724688950297774E-5 44-45 2.908229650099258E-5 0.0 0.0 0.10263142435200281 1.0178803775347402E-4 46-47 2.908229650099258E-5 0.0 0.0 0.1324844017102717 1.1632918600397031E-4 48-49 2.908229650099258E-5 0.0 0.0 0.16517290297738735 1.1632918600397031E-4 50-51 2.908229650099258E-5 0.0 0.0 0.2014821501588766 1.1632918600397031E-4 52-53 4.3623444751488864E-5 0.0 0.0 0.23802405571237376 1.1632918600397031E-4 54-55 7.270574125248145E-5 0.0 0.0 0.28060053778982685 1.1632918600397031E-4 56-57 8.724688950297774E-5 0.0 0.0 0.33560970162145437 1.1632918600397031E-4 58-59 8.724688950297774E-5 0.0 0.0 0.39419598792270394 1.1632918600397031E-4 60-61 8.724688950297774E-5 0.0 0.0 0.4570282895130984 1.1632918600397031E-4 62-63 8.724688950297774E-5 0.0 0.0 0.5227397384570911 1.1632918600397031E-4 64-65 8.724688950297774E-5 0.0 0.0 0.5962161605668488 1.1632918600397031E-4 66-67 8.724688950297774E-5 0.0 0.0 0.6880435117687329 1.1632918600397031E-4 68-69 1.0178803775347402E-4 0.0 0.0 0.7900351255977138 1.1632918600397031E-4 70-71 1.1632918600397031E-4 0.0 0.0 0.9074112742757199 1.1632918600397031E-4 72-73 1.1632918600397031E-4 0.0 0.0 1.0323633611922345 1.1632918600397031E-4 74-75 1.1632918600397031E-4 0.0 0.0 1.1665490772478142 1.1632918600397031E-4 76-77 1.1632918600397031E-4 0.0 0.0 1.3158285051874092 1.1632918600397031E-4 78-79 1.1632918600397031E-4 0.0 0.0 1.488039323918037 1.1632918600397031E-4 80-81 1.1632918600397031E-4 0.0 0.0 1.6822799822481662 1.1632918600397031E-4 82-83 1.1632918600397031E-4 1.454114825049629E-5 0.0 1.890247484526764 1.1632918600397031E-4 84-85 1.1632918600397031E-4 2.908229650099258E-5 0.0 2.101239545641465 1.1632918600397031E-4 86-87 1.1632918600397031E-4 2.908229650099258E-5 0.0 2.338827366906324 1.1632918600397031E-4 88 1.454114825049629E-4 2.908229650099258E-5 0.0 2.531235840556891 1.454114825049629E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3180 0.0 27.177004 1 GTACATG 6265 0.0 25.42139 1 TCAACGC 3200 0.0 25.212376 4 ATCAACG 3240 0.0 24.556684 3 CAACGCA 3140 0.0 23.046392 5 TACATGG 6655 0.0 22.43844 2 GGTATCA 2370 0.0 21.618742 1 TATCAAC 3710 0.0 21.455626 2 AACGCAG 3320 0.0 21.249264 6 ACATGGG 6900 0.0 20.869423 3 ACGCAGA 3740 0.0 18.938696 7 CATGGGG 5100 0.0 18.89555 4 CTATTAG 1035 0.0 18.489367 1 CGCAGAG 3815 0.0 18.114925 8 GTATAGA 1250 0.0 17.284575 1 TAGACCG 220 3.917694E-4 15.879615 5 GTATAGC 670 3.6379788E-12 15.662994 1 CCTATAC 995 0.0 15.204324 3 GTATTAA 1870 0.0 15.185073 1 TAGTGCG 230 5.5148476E-4 15.181112 7 >>END_MODULE