FastQCFastQC Report
Wed 25 May 2016
SRR1295163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438518
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52100.034.2688331
GGTATCA31950.031.510821
CAACGCA58950.029.5079275
ATCAACG59900.029.431513
TCAACGC60150.029.3100434
AACGCAG62300.027.8895366
TATCAAC63800.027.6556342
ACGCAGA73400.023.4775837
CGCAGAG74450.023.020338
GTACATG80350.022.528981
TACATGG86150.020.7685552
ACATGGG88850.019.5772863
GCAGAGT93000.018.0244649
CATGGGG61350.017.8550954
AGAGTAC80700.016.66971410-11
GTACTAG7200.016.5315381
TATACTG17750.015.097235
GTATCGT2801.4922676E-515.0942696
GAGTACT41050.015.04112112-13
CAGAGTA88950.014.88607710-11
Produced by FastQC (version 0.11.5)