FastQCFastQC Report
Wed 25 May 2016
SRR1295162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682823
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34970.13034777173149328No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29930.11156159016081195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG1951.5725938E-417.8966085
GTCTAGG7200.017.1722221
GTATAGG8350.017.0282651
TATACTG13150.016.3023415
GTATTAC9650.015.3749231
GTACATG135450.015.3351951
CCTACAC7950.014.7700473
TACATGG137300.014.76594452
GTATTAG10650.014.5117371
GTATATA17500.014.1302851
ACATGGG137100.013.988993
GTATAAA18200.013.9264821
TAACGGT2450.00687366613.69385054
ATCGTTC2200.006955393613.6695158
GTCCTAG7352.8376235E-1013.4574151
GTATATG15650.013.4305431
CCAGTAC10450.013.3768363
CTAGTAC5951.1732482E-813.1565123
CTATACT11500.013.1282344
CTACACT9450.013.0176114
Produced by FastQC (version 0.11.5)