##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2682823 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.442363137635244 33.0 27.0 33.0 15.0 33.0 2 22.62741112626513 27.0 6.0 33.0 2.0 33.0 3 24.947473985425056 27.0 15.0 33.0 15.0 33.0 4 25.1607810131343 33.0 6.0 37.0 2.0 37.0 5 27.148019082883962 33.0 15.0 37.0 2.0 37.0 6 27.889190229843713 33.0 15.0 37.0 6.0 37.0 7 27.749489996171942 33.0 15.0 37.0 6.0 37.0 8 28.38058828331202 33.0 22.0 37.0 6.0 37.0 9 29.42741470458543 33.0 27.0 37.0 15.0 37.0 10-11 28.931287304455047 35.0 24.5 37.0 10.5 37.0 12-13 27.748828006916597 33.0 15.0 37.0 4.0 37.0 14-15 29.555624989050713 33.0 27.0 37.0 10.5 40.0 16-17 29.959536279508562 35.0 27.0 40.0 6.0 40.0 18-19 30.11314872431018 37.0 27.0 40.0 10.5 40.0 20-21 29.663735736573006 33.0 27.0 40.0 6.0 40.0 22-23 30.40098619998412 35.0 27.0 40.0 15.0 40.0 24-25 30.532075914065146 37.0 27.0 40.0 15.0 40.0 26-27 29.129075604316796 33.0 22.0 38.5 4.0 40.0 28-29 29.81032255948305 33.0 27.0 40.0 10.5 40.0 30-31 30.471001627762995 33.0 27.0 40.0 15.0 40.0 32-33 29.28372427103838 33.0 24.5 38.5 6.0 40.0 34-35 28.920406228811963 33.0 22.0 37.0 6.0 40.0 36-37 29.264678288504314 33.0 22.0 37.0 6.0 40.0 38-39 29.357046849531258 33.0 24.5 37.0 6.0 40.0 40-41 28.430501192214322 33.0 22.0 37.0 6.0 40.0 42-43 29.070252677869544 33.0 22.0 37.0 6.0 40.0 44-45 28.970197810291623 33.0 22.0 37.0 6.0 40.0 46-47 28.662653108311655 33.0 22.0 37.0 6.0 40.0 48-49 28.577080560290412 33.0 22.0 37.0 6.0 40.0 50-51 28.56681823586573 33.0 22.0 37.0 6.0 40.0 52-53 28.758399454604348 33.0 22.0 37.0 6.0 40.0 54-55 28.739716522483967 33.0 22.0 37.0 6.0 40.0 56-57 28.732550376972316 33.0 22.0 37.0 6.0 40.0 58-59 28.58243574026315 33.0 22.0 37.0 6.0 40.0 60-61 28.539984001926328 33.0 22.0 37.0 10.5 40.0 62-63 28.483290735169632 33.0 22.0 37.0 6.0 40.0 64-65 28.06012267674759 33.0 22.0 37.0 6.0 40.0 66-67 28.254017689575498 33.0 22.0 37.0 10.5 40.0 68-69 27.85966330987918 33.0 22.0 37.0 6.0 38.5 70-71 27.963053470169296 33.0 22.0 37.0 10.5 37.0 72-73 27.988245031446354 33.0 22.0 37.0 10.5 37.0 74-75 28.065456796814402 33.0 22.0 37.0 15.0 37.0 76-77 27.80330606976308 33.0 22.0 37.0 10.5 37.0 78-79 27.380021715931314 33.0 22.0 37.0 10.5 37.0 80-81 27.199184590261822 30.0 22.0 35.0 6.0 37.0 82-83 27.288132873469475 27.0 22.0 33.0 15.0 37.0 84-85 27.241870037643185 30.0 22.0 33.0 10.5 37.0 86-87 27.017019385922964 27.0 22.0 33.0 15.0 37.0 88-89 26.936420330375874 27.0 22.0 33.0 15.0 37.0 90-91 27.084730710896693 27.0 22.0 33.0 15.0 37.0 92-93 27.017457171047063 27.0 22.0 33.0 15.0 37.0 94-95 26.895524974998352 27.0 22.0 33.0 15.0 37.0 96-97 26.9902904515132 27.0 22.0 33.0 15.0 37.0 98-99 27.0530234383707 27.0 22.0 33.0 15.0 37.0 100 26.708991610702608 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.304909475074371 1101 2 11.303739856619178 1101 3 8.342604987849974 1101 4 12.760988094258192 1101 5 10.829458414232281 1101 6 9.952026780563756 1101 7 9.430148832917379 1101 8 8.985237012296356 1101 9 7.257101960232323 1101 10-11 7.79127074689443 1101 12-13 9.147226818795982 1101 14-15 8.089551197439786 1101 16-17 8.243463905875213 1101 18-19 8.212146232835636 1101 20-21 7.151364070041783 1101 22-23 7.350125643148214 1101 24-25 7.318834973485387 1101 26-27 8.657077667923776 1101 28-29 8.133389769969607 1101 30-31 5.530791537088415 1101 32-33 7.029951529803707 1101 34-35 8.632031160557752 1101 36-37 8.106110659783777 1101 38-39 8.107961374386683 1101 40-41 8.879560289982138 1101 42-43 8.632040528490233 1101 44-45 8.547810443961524 1101 46-47 7.6132378312772815 1101 48-49 5.701617562279683 1101 50-51 6.954914522369442 1101 52-53 6.126327560925358 1101 54-55 4.2804525642806475 1101 56-57 6.204565696413496 1101 58-59 6.202584813965689 1101 60-61 6.867664097825273 1101 62-63 6.34036186151878 1101 64-65 6.890915980571528 1101 66-67 5.9210191639459495 1101 68-69 6.783643954539713 1101 70-71 6.299008593096659 1101 72-73 5.549296507488307 1101 74-75 4.968991528245976 1101 76-77 5.132496362046055 1101 78-79 5.265903098739628 1101 80-81 5.069507977544557 1101 82-83 4.538878027484216 1101 84-85 4.23514694237123 1101 86-87 4.125446892374228 1101 88-89 4.239306104117347 1101 90-91 3.9862969908756085 1101 92-93 3.842813585638254 1101 94-95 3.748178257519889 1101 96-97 3.8984442146313647 1101 98-99 3.7383869706769275 1101 100 3.980497217645464 1102 1 6.6968416026643105 1102 2 10.39942388846271 1102 3 8.058091257240012 1102 4 12.53322427955035 1102 5 10.615416943671033 1102 6 9.51589877896572 1102 7 6.782035471871065 1102 8 7.833783387523798 1102 9 6.496335261485125 1102 10-11 7.505023902337651 1102 12-13 9.023914732192456 1102 14-15 6.11044183816605 1102 16-17 7.311563790632253 1102 18-19 7.402885048705997 1102 20-21 6.934638343614754 1102 22-23 6.629212700475417 1102 24-25 6.974057466774816 1102 26-27 8.51837729552081 1102 28-29 7.715736799431415 1102 30-31 4.231798115847479 1102 32-33 4.292346680356605 1102 34-35 4.989016805513344 1102 36-37 5.24784941454153 1102 38-39 4.486184079435731 1102 40-41 6.392540415377869 1102 42-43 7.014598919321127 1102 44-45 6.5811880688918905 1102 46-47 5.638423387397555 1102 48-49 3.8373538730489365 1102 50-51 5.102384052234722 1102 52-53 5.810160580773665 1102 54-55 5.360977131619592 1102 56-57 6.384260951660046 1102 58-59 6.049624303624714 1102 60-61 6.209217678237476 1102 62-63 4.4197898257587624 1102 64-65 6.187319321817977 1102 66-67 3.7551547828735323 1102 68-69 6.05615428702523 1102 70-71 5.217849133691946 1102 72-73 5.024839704144004 1102 74-75 4.286528141300586 1102 76-77 3.427507034977207 1102 78-79 4.3805230704479 1102 80-81 4.472498249122616 1102 82-83 3.9614163820904906 1102 84-85 3.0848178697583073 1102 86-87 3.2725851592568453 1102 88-89 3.5663544801737785 1102 90-91 3.3724915960450588 1102 92-93 3.264043864037543 1102 94-95 3.1030850711112308 1102 96-97 3.441020475670488 1102 98-99 3.2280948544293473 1102 100 3.2268300960294027 1103 1 6.624426021000133 1103 2 10.862841157145976 1103 3 7.935309557133792 1103 4 12.091045957191586 1103 5 10.535818223603481 1103 6 9.048097911821976 1103 7 8.07120098364815 1103 8 8.010210278332362 1103 9 7.043623746510708 1103 10-11 7.51656077681508 1103 12-13 8.767813627675071 1103 14-15 8.566008047382699 1103 16-17 7.979668010831137 1103 18-19 7.340988242826235 1103 20-21 8.380454292615678 1103 22-23 6.877761957232654 1103 24-25 5.172484162831246 1103 26-27 6.096875398539165 1103 28-29 7.091249416245585 1103 30-31 6.16679025771921 1103 32-33 7.9307625448316905 1103 34-35 7.198390829052251 1103 36-37 4.189256179247945 1103 38-39 6.492310424429824 1103 40-41 6.869577827392948 1103 42-43 7.541653859322569 1103 44-45 5.819690748850029 1103 46-47 6.540390007774583 1103 48-49 5.699217527564876 1103 50-51 6.592628677791403 1103 52-53 6.302777767053204 1103 54-55 5.011320050867141 1103 56-57 6.012067850653139 1103 58-59 5.537838375081002 1103 60-61 5.088690911619402 1103 62-63 2.8172956052033484 1103 64-65 5.14272087641972 1103 66-67 4.242077570646046 1103 68-69 5.112922169252684 1103 70-71 3.7887789440631465 1103 72-73 4.4572637983518355 1103 74-75 3.3373962917222464 1103 76-77 3.7113958836478886 1103 78-79 3.0351007024094514 1103 80-81 4.251687387161585 1103 82-83 3.504595159057061 1103 84-85 3.5084171438083978 1103 86-87 2.934646610933875 1103 88-89 3.116421004811876 1103 90-91 2.7035643966927907 1103 92-93 2.4316340014906395 1103 94-95 2.906276881128374 1103 96-97 3.040029261942891 1103 98-99 2.8918541855800797 1103 100 3.0464742145662633 1104 1 6.4645697534045965 1104 2 9.816186949039583 1104 3 7.400186601509905 1104 4 12.23576618815935 1104 5 9.671414962967692 1104 6 8.219651396682792 1104 7 4.003418659448506 1104 8 6.7031577196289085 1104 9 6.659104627803682 1104 10-11 7.1913112332333675 1104 12-13 7.655736386781161 1104 14-15 7.371288367410752 1104 16-17 7.212836148353883 1104 18-19 6.914161879408283 1104 20-21 7.603283584061696 1104 22-23 6.653186311804635 1104 24-25 6.0990501504531025 1104 26-27 8.319039771246782 1104 28-29 7.300516884266035 1104 30-31 6.495046119224778 1104 32-33 7.751640057735894 1104 34-35 8.040050693039845 1104 36-37 6.751255439097083 1104 38-39 7.4268185323029385 1104 40-41 7.2626030801970956 1104 42-43 7.312422632294858 1104 44-45 6.903496742739911 1104 46-47 6.824355614858135 1104 48-49 5.705377857537496 1104 50-51 5.950220479748737 1104 52-53 6.1804578167421305 1104 54-55 4.981137302509715 1104 56-57 6.1130516082653585 1104 58-59 4.154393985816871 1104 60-61 4.491604087031281 1104 62-63 4.083540596590858 1104 64-65 2.961252237042725 1104 66-67 3.468128689010122 1104 68-69 4.214170692462805 1104 70-71 2.6942000737958978 1104 72-73 2.6946874097455087 1104 74-75 3.3823095801185445 1104 76-77 4.030633881263711 1104 78-79 4.479669433748249 1104 80-81 3.1933925448214673 1104 82-83 3.1002427190054007 1104 84-85 3.1315181458215307 1104 86-87 1.0280568402740755 1104 88-89 0.5184452604952483 1104 90-91 2.4867355707474026 1104 92-93 2.6240455690846254 1104 94-95 2.596413184171709 1104 96-97 2.9292384297624103 1104 98-99 2.6892711747306706 1104 100 1.0375793256760844 1105 1 5.869129585832223 1105 2 -2.795247230697022 1105 3 0.41056257571168686 1105 4 8.611805961216259 1105 5 8.849149859213991 1105 6 8.199982250596037 1105 7 5.312901716073082 1105 8 6.06843377167014 1105 9 4.992326902939542 1105 10-11 5.010307473007874 1105 12-13 5.19114686899896 1105 14-15 5.977440222020203 1105 16-17 6.143012290321433 1105 18-19 6.373954057752485 1105 20-21 7.227243236878426 1105 22-23 2.0886822179815105 1105 24-25 4.198839929553451 1105 26-27 7.157566822872223 1105 28-29 6.207871473167383 1105 30-31 5.4262881116729 1105 32-33 7.310455405402646 1105 34-35 6.33105289405901 1105 36-37 6.436455307591924 1105 38-39 4.403846376827104 1105 40-41 5.693279195573524 1105 42-43 5.824748309401354 1105 44-45 6.354531167356512 1105 46-47 5.671531245551051 1105 48-49 5.265682307681576 1105 50-51 5.96407517622324 1105 52-53 5.765451060181373 1105 54-55 5.414530334912197 1105 56-57 5.297037290158258 1105 58-59 2.0686299324122395 1105 60-61 4.613633798976977 1105 62-63 -0.761637346320434 1105 64-65 -0.12927150519750086 1105 66-67 1.0479889406104235 1105 68-69 2.9928474278957076 1105 70-71 3.148732378375371 1105 72-73 2.8059327925608777 1105 74-75 2.8592516682946822 1105 76-77 2.1028031059151253 1105 78-79 3.494191325488565 1105 80-81 1.8484715083481298 1105 82-83 1.3259462051006672 1105 84-85 1.578162951367954 1105 86-87 -0.13644142163391138 1105 88-89 1.7435265447010906 1105 90-91 1.5546048306924796 1105 92-93 1.8073926713782278 1105 94-95 1.2351847229754007 1105 96-97 2.1180078231465416 1105 98-99 2.0478516140642498 1105 100 2.2474857249489375 1106 1 6.262484161452164 1106 2 10.416716251690833 1106 3 6.903152565308812 1106 4 11.899762153592679 1106 5 10.133395150143347 1106 6 9.380082451216538 1106 7 6.5483729668386275 1106 8 7.49431464558263 1106 9 6.8630990651935555 1106 10-11 5.8515610592733545 1106 12-13 8.090113840838317 1106 14-15 7.996518843270568 1106 16-17 7.823919135862614 1106 18-19 7.319521769911404 1106 20-21 8.241131887909273 1106 22-23 6.322400639363966 1106 24-25 5.217559516851814 1106 26-27 7.8540182607709745 1106 28-29 6.486947964065688 1106 30-31 5.711209189388082 1106 32-33 7.647771499575466 1106 34-35 6.946542849849017 1106 36-37 6.272321162374215 1106 38-39 7.22859329011289 1106 40-41 6.768348535718452 1106 42-43 6.431476666402144 1106 44-45 4.768363315169168 1106 46-47 6.307370804262991 1106 48-49 6.123458966785837 1106 50-51 6.677935827494679 1106 52-53 5.796515045113058 1106 54-55 5.411068364659499 1106 56-57 5.008127639307851 1106 58-59 5.204006695100798 1106 60-61 5.125720939561546 1106 62-63 5.185376412672454 1106 64-65 5.358260127864021 1106 66-67 3.3191593809236757 1106 68-69 3.4483054698858666 1106 70-71 3.034069082148882 1106 72-73 2.9605919969706775 1106 74-75 3.0783828076123 1106 76-77 3.7302187073810664 1106 78-79 4.326453700122979 1106 80-81 3.837677112211086 1106 82-83 2.215881654544212 1106 84-85 2.8109685361495984 1106 86-87 3.4198542872325888 1106 88-89 3.5217064062667234 1106 90-91 3.4457974397041333 1106 92-93 3.0887190688699278 1106 94-95 1.6136673819392193 1106 96-97 1.633834398266341 1106 98-99 2.020321776542854 1106 100 3.052573977523952 1107 1 6.169821697923112 1107 2 10.336747672499264 1107 3 6.162415618263122 1107 4 11.442151955549942 1107 5 9.476518466793902 1107 6 7.942086216105629 1107 7 3.8549766182819774 1107 8 6.259269722120884 1107 9 5.731642584162447 1107 10-11 4.671298102517792 1107 12-13 6.181387682982962 1107 14-15 6.197023215081739 1107 16-17 6.941163273727728 1107 18-19 6.336181567268582 1107 20-21 5.611156303227087 1107 22-23 5.844870614583602 1107 24-25 4.685287277519912 1107 26-27 7.208572298468599 1107 28-29 6.502510234559448 1107 30-31 5.156609538848468 1107 32-33 5.155418359244887 1107 34-35 6.757224278785706 1107 36-37 6.536488896421741 1107 38-39 6.440831777881549 1107 40-41 7.717582344900997 1107 42-43 6.103850885220865 1107 44-45 4.28082543195362 1107 46-47 3.459080530473372 1107 48-49 4.084829174913423 1107 50-51 4.992096929325061 1107 52-53 3.961339337797316 1107 54-55 5.402014969418417 1107 56-57 5.314790338498845 1107 58-59 3.2833354336043072 1107 60-61 2.888507047428231 1107 62-63 0.5792304150736882 1107 64-65 2.221275011217678 1107 66-67 4.230801451171217 1107 68-69 4.999417173793127 1107 70-71 4.296114506708211 1107 72-73 2.531129998369895 1107 74-75 2.6443223444234256 1107 76-77 3.1546402650347893 1107 78-79 3.9436927238476827 1107 80-81 2.172975250284825 1107 82-83 1.5437460662198177 1107 84-85 2.1783372430124466 1107 86-87 2.3002969560579345 1107 88-89 2.5952423814376324 1107 90-91 1.4691379209794313 1107 92-93 2.335209467967747 1107 94-95 2.252433997641809 1107 96-97 2.4393294862961277 1107 98-99 1.895175983127615 1107 100 0.10413418272795383 1108 1 4.5591303157403225 1108 2 -3.2634830428139665 1108 3 0.04542928507405364 1108 4 6.799900041661552 1108 5 7.2997527986245565 1108 6 6.809647352276944 1108 7 4.255549657849958 1108 8 5.161491933573174 1108 9 4.931453332441016 1108 10-11 3.4435326543664964 1108 12-13 2.600300887849425 1108 14-15 4.135536492189797 1108 16-17 4.840987854881803 1108 18-19 4.2415142633982015 1108 20-21 4.898958591554898 1108 22-23 4.234634377214494 1108 24-25 3.1210869987369634 1108 26-27 4.738947926728727 1108 28-29 6.132306706773296 1108 30-31 4.595995954182282 1108 32-33 4.648206899948345 1108 34-35 3.211271118922504 1108 36-37 1.3043245763417382 1108 38-39 2.7374162926051255 1108 40-41 5.42678530325616 1108 42-43 3.2412272798071164 1108 44-45 3.8352705701504135 1108 46-47 4.812263826058739 1108 48-49 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-17.459994013110187 2213 42-43 -23.660277508154582 2213 44-45 -17.509214694729557 2213 46-47 -17.338638248112066 2213 48-49 -6.233087732350143 2213 50-51 -23.852448059577107 2213 52-53 -18.216746934112784 2213 54-55 -18.159056426117544 2213 56-57 -17.93852539466576 2213 58-59 -18.46541930802778 2213 60-61 -19.795240991131884 2213 62-63 -17.95894535846532 2213 64-65 -18.08816840902469 2213 66-67 -8.883590289983527 2213 68-69 -23.262918492930453 2213 70-71 -17.882829573248557 2213 72-73 -9.618911759770857 2213 74-75 -4.416966036637689 2213 76-77 -3.1555494052531436 2213 78-79 -4.870263394781304 2213 80-81 -3.3215760067173683 2213 82-83 -3.3342383813854113 2213 84-85 -3.052546949439826 2213 86-87 -3.3399435871807377 2213 88-89 -2.9377787868911724 2213 90-91 -2.9145261842599233 2213 92-93 -3.5920519211449786 2213 94-95 -3.5525548177823687 2213 96-97 -3.392822055093479 2213 98-99 -4.128781921263467 2213 100 -3.3782731413488314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 712.0 3 662.0 4 3715.0 5 12975.0 6 19924.0 7 22393.0 8 19991.0 9 20164.0 10 23603.0 11 30011.0 12 37614.0 13 50613.0 14 40678.0 15 44345.0 16 49748.0 17 52691.0 18 52986.0 19 53822.0 20 53248.0 21 52002.0 22 53704.0 23 58748.0 24 65080.0 25 71370.0 26 78100.0 27 83797.0 28 90860.0 29 96420.0 30 100954.0 31 110403.0 32 124286.0 33 151334.0 34 184461.0 35 220202.0 36 247314.0 37 225197.0 38 78460.0 39 236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.875917700565 21.218840068373424 14.30746936164934 23.597772869412236 2 14.737814556193825 21.381319993513547 40.73729367423256 23.143571776060067 3 17.7006513265275 27.14573792636686 30.014395992421996 25.139214754683653 4 11.93700242535189 17.85128385315748 39.40612842392199 30.805585297568637 5 12.909554457247996 37.798399504152805 36.16390730299261 13.128138735606587 6 29.91728951135703 38.38925071732402 18.606347778400796 13.087111992918157 7 25.431407195000048 32.22055512363566 24.843246736200157 17.50479094516412 8 23.575651807916355 36.65618368692828 22.433481476285234 17.334683028870135 9 24.030992562239657 17.53526754030338 22.6277369402232 35.806002957233765 10-11 22.874772699091835 27.950658805737515 29.235763779970924 19.938804715199723 12-13 23.574344638887965 25.66738485458812 29.455669473515023 21.30260103300889 14-15 21.235833322798065 26.935073238186803 26.664828512895472 25.16426492611966 16-17 20.696777991834438 30.71200513305275 27.677115960878645 20.914100914234165 18-19 20.741968302161187 29.668367062185364 29.233315101521427 20.356349534132026 20-21 22.15512254627101 28.9981129350677 28.116682573733897 20.730081944927395 22-23 21.028667038571065 29.06030181107967 28.661629770354956 21.249401379994307 24-25 21.358592094453112 28.71547429982516 28.128990984792434 21.796942620929293 26-27 21.450099524824008 28.716522766843607 28.180154540220826 21.653223168111563 28-29 21.114946955499793 28.56563462821205 28.83323936727758 21.486179049010577 30-31 21.902102325465595 29.037140227388196 27.95510899866256 21.105648448483652 32-33 20.80102664494761 28.893615322606596 28.78524407664888 21.520113955796916 34-35 20.97196636339686 28.228117093978966 28.625819895702286 22.17409664692189 36-37 21.703287343217553 28.918960975513535 28.10240015046891 21.275351530800002 38-39 21.383392808853348 28.45237477773392 28.4752200290693 21.68901238434344 40-41 21.613522655382894 28.640880118036876 27.945189621609767 21.800407604970466 42-43 21.254329646014185 28.846470235720318 28.59159933427967 21.307600783985833 44-45 21.822611882382517 28.143036149558753 28.34882997614143 21.6855219919173 46-47 21.55483361262929 28.784473411789268 28.54127240331865 21.119420572262786 48-49 21.403393191553217 28.378558373445728 28.732682293358074 21.485366141642974 50-51 21.69795778355506 28.51034678163577 28.278921583647943 21.512773851161224 52-53 20.92157178555994 28.7327063604914 28.444116301521106 21.90160555242755 54-55 21.52594705296112 28.611595248190223 27.994072754068966 21.868384944779688 56-57 21.68038876969178 28.55026447170327 28.043065691434183 21.72628106717077 58-59 21.314430888733753 27.945463108089786 28.6740369485086 22.06606905466786 60-61 21.877002972919076 28.530741878354387 28.030329949164656 21.561925199561877 62-63 21.12933840000307 28.695089198234093 28.5497170170174 21.625855384745428 64-65 21.133632521288874 28.16323029414517 28.54377865809913 22.15935852646683 66-67 21.536459407792492 28.401938267020576 28.201964703491296 21.859637621695633 68-69 21.923409430488867 28.24274557058762 28.12749592473099 21.70634907419252 70-71 21.925031358523025 28.445675273900083 27.78582854488814 21.843464822688752 72-73 21.587701094097202 28.571198814475746 28.36170372186802 21.47939636955903 74-75 21.97207900503935 27.95227251636483 27.993130584640447 22.082517893955373 76-77 21.810613276497826 28.492526193687105 28.215321067174663 21.481539462640402 78-79 21.7655727120841 28.19314192950857 28.45872923742645 21.582556120980886 80-81 21.922437958874422 28.21081488161023 28.052335069753386 21.814412089761962 82-83 21.2571126878985 28.534567874559286 28.41020563265697 21.798113804885244 84-85 21.63782607021837 28.077064177246626 27.881480547694554 22.40362920484045 86-87 21.863421107020798 28.323806877918596 28.000477758041757 21.81229425701885 88-89 21.668563578209245 27.79760025823786 28.501595114512302 22.032241049040593 90-91 22.089663445084422 27.997057075455643 27.948675366023433 21.9646041134365 92-93 21.376906668512046 28.341564274752905 28.45627064943858 21.82525840729647 94-95 21.387310100311634 27.817711290633895 28.385625434518218 22.409353174536246 96-97 21.76379558739092 28.107914332359385 28.20331672770721 21.92497335254249 98-99 21.716081560838155 27.807134447973404 28.288721576987186 22.18806241420126 100 21.840893380455842 27.72229474335383 28.04702964179554 22.38978223439479 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 379.0 1 411.0 2 383.5 3 323.0 4 364.5 5 488.0 6 677.0 7 984.5 8 1467.0 9 2071.5 10 2813.0 11 3801.0 12 4764.5 13 5567.0 14 6469.5 15 7307.0 16 8007.0 17 8785.5 18 9572.5 19 10283.0 20 11138.0 21 12179.5 22 13539.0 23 15391.0 24 17612.5 25 20814.0 26 25184.5 27 30551.5 28 37071.5 29 44307.5 30 52131.0 31 60158.5 32 68111.0 33 75501.0 34 82036.0 35 88214.5 36 93573.0 37 97725.0 38 100828.5 39 103052.0 40 103461.0 41 102429.5 42 100622.0 43 96990.5 44 92747.5 45 88804.5 46 84479.0 47 80544.5 48 77954.5 49 77437.0 50 79122.5 51 75428.0 52 65398.5 53 57176.5 54 51281.5 55 46382.5 56 42317.0 57 38276.5 58 34065.5 59 30054.5 60 25876.0 61 21727.5 62 18126.5 63 14882.0 64 11928.5 65 9500.0 66 7554.5 67 5947.0 68 4610.5 69 3483.0 70 2702.0 71 2061.5 72 1519.0 73 1169.5 74 903.5 75 651.5 76 430.5 77 285.5 78 192.0 79 133.0 80 98.5 81 66.0 82 44.5 83 33.5 84 18.0 85 14.0 86 10.0 87 6.0 88 6.0 89 2.5 90 1.5 91 1.5 92 1.0 93 1.0 94 2.0 95 1.5 96 1.5 97 1.5 98 0.0 99 1.0 100 3.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.4543493924123965 2 24.146691749697986 3 5.9341969261483145 4 23.72582909867703 5 11.957740037266715 6 9.25994745087544 7 7.7082237628050745 8 8.607053092954697 9 4.887165496941096 10-11 6.661210970682747 12-13 9.78862563799401 14-15 7.12654170625494 16-17 7.690555806327887 18-19 6.50013064596509 20-21 7.598004042756455 22-23 5.587919888863335 24-25 5.086395934431753 26-27 9.42201554109235 28-29 6.119785017498359 30-31 4.183690090624689 32-33 8.492397746701888 34-35 6.833976747627406 36-37 6.46089958226838 38-39 5.857039394697302 40-41 8.233137258775551 42-43 6.204322834566425 44-45 6.223127653221998 46-47 5.734444650280693 48-49 3.873662183453772 50-51 6.333477832864859 52-53 4.305669811239877 54-55 4.702322888986713 56-57 4.605969160097405 58-59 3.5190543692222707 60-61 4.01608678619499 62-63 2.9891274974159683 64-65 4.402191273893209 66-67 2.4312822724421252 68-69 4.8656769380611395 70-71 2.4121978975131793 72-73 1.5013662846933995 74-75 0.7890941743081821 76-77 1.1889714677412562 78-79 1.6804686705011846 80-81 2.075220765589083 82-83 0.8068180420400451 84-85 1.241863514663472 86-87 0.447849149943921 88-89 0.7515031740819278 90-91 0.3463515856245455 92-93 0.35319139577974396 94-95 0.5456192972849868 96-97 0.546103861492167 98-99 0.47313967414175295 100 0.4598514326140785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2682823.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.02110577319822 #Duplication Level Percentage of deduplicated Percentage of total 1 96.43767001953782 90.6717637342775 2 2.806588104107625 5.277570339962057 3 0.44303575070398477 1.2496413353474287 4 0.13400757028223803 0.5039815975966238 5 0.05666142479153921 0.26636849067927115 6 0.036562907882435214 0.20626110176340862 7 0.018988270398722817 0.12497087257258843 8 0.014228235241379394 0.10702035284765428 9 0.010033787347781802 0.08490490033784136 >10 0.03932928139033815 0.6038134585844152 >50 0.0011731321443952847 0.07619786922517244 >100 0.0012821275085894382 0.2767444341559613 >500 2.3966654353900797E-4 0.1719697897368668 >1k 1.9972211960554023E-4 0.3787917229132177 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3497 0.13034777173149328 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2993 0.11156159016081195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 3.7274169783097875E-5 0.0 0.0 0.0 8 0.0 3.7274169783097875E-5 0.0 0.0 0.0 9 0.0 3.7274169783097875E-5 0.0 3.7274169783097875E-5 0.0 10-11 0.0 3.7274169783097875E-5 0.0 3.7274169783097875E-5 0.0 12-13 0.0 3.7274169783097875E-5 0.0 5.591125467464681E-5 0.0 14-15 0.0 3.7274169783097875E-5 0.0 9.318542445774469E-5 0.0 16-17 0.0 3.7274169783097875E-5 0.0 1.3045959424084257E-4 0.0 18-19 0.0 3.7274169783097875E-5 0.0 1.8637084891548938E-4 0.0 20-21 0.0 3.7274169783097875E-5 0.0 2.4228210359013622E-4 0.0 22-23 0.0 3.7274169783097875E-5 0.0 2.6091918848168515E-4 3.7274169783097875E-5 24-25 0.0 5.591125467464681E-5 0.0 3.5410461293942984E-4 3.7274169783097875E-5 26-27 0.0 7.454833956619575E-5 0.0 5.032012920718214E-4 3.7274169783097875E-5 28-29 0.0 9.318542445774469E-5 0.0 9.504913294689958E-4 3.7274169783097875E-5 30-31 0.0 1.1182250934929363E-4 0.0 0.0024600952056844597 3.7274169783097875E-5 32-33 0.0 1.1182250934929363E-4 0.0 0.006429794287584384 3.7274169783097875E-5 34-35 0.0 1.1182250934929363E-4 0.0 0.013101870678758905 3.7274169783097875E-5 36-37 0.0 1.3045959424084257E-4 0.0 0.023147259435303782 3.7274169783097875E-5 38-39 0.0 1.6773376402394046E-4 0.0 0.03928697495138517 3.7274169783097875E-5 40-41 0.0 1.8637084891548938E-4 0.0 0.060328243793943914 3.7274169783097875E-5 42-43 0.0 1.8637084891548938E-4 0.0 0.08090358551421395 3.7274169783097875E-5 44-45 0.0 1.8637084891548938E-4 0.0 0.10462859458115575 3.7274169783097875E-5 46-47 0.0 2.6091918848168515E-4 0.0 0.1335160761630566 7.454833956619575E-5 48-49 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.16417408080965462 7.454833956619575E-5 50-51 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.1988576957928272 7.454833956619575E-5 52-53 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.23594549472700957 7.454833956619575E-5 54-55 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.2777857503085369 7.454833956619575E-5 56-57 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.32555259888557686 7.454833956619575E-5 58-59 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.3806624589098871 7.454833956619575E-5 60-61 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.4400588484592536 7.454833956619575E-5 62-63 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.5026421795250748 7.454833956619575E-5 64-65 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.5719721353216369 7.454833956619575E-5 66-67 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.6575163549738465 7.454833956619575E-5 68-69 3.7274169783097875E-5 2.6091918848168515E-4 0.0 0.7556965181825264 7.454833956619575E-5 70-71 5.591125467464681E-5 2.6091918848168515E-4 0.0 0.8758125303085593 7.454833956619575E-5 72-73 7.454833956619575E-5 2.6091918848168515E-4 0.0 0.997382235056133 7.454833956619575E-5 74-75 7.454833956619575E-5 2.6091918848168515E-4 0.0 1.1226420826122334 7.454833956619575E-5 76-77 7.454833956619575E-5 2.98193358264783E-4 0.0 1.2637248152412588 7.454833956619575E-5 78-79 1.1182250934929363E-4 2.98193358264783E-4 0.0 1.421394553423763 7.454833956619575E-5 80-81 1.1182250934929363E-4 3.354675280478809E-4 0.0 1.600590124656006 9.318542445774469E-5 82-83 1.1182250934929363E-4 3.7274169783097877E-4 0.0 1.7941735254245248 1.1182250934929363E-4 84-85 1.1182250934929363E-4 4.100158676140767E-4 0.0 1.9913166094073296 1.1182250934929363E-4 86-87 1.1182250934929363E-4 4.100158676140767E-4 0.0 2.210731009835535 1.1182250934929363E-4 88 1.1182250934929363E-4 4.100158676140767E-4 0.0 2.3964309236949286 1.1182250934929363E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTCG 195 1.5725938E-4 17.896608 5 GTCTAGG 720 0.0 17.172222 1 GTATAGG 835 0.0 17.028265 1 TATACTG 1315 0.0 16.302341 5 GTATTAC 965 0.0 15.374923 1 GTACATG 13545 0.0 15.335195 1 CCTACAC 795 0.0 14.770047 3 TACATGG 13730 0.0 14.7659445 2 GTATTAG 1065 0.0 14.511737 1 GTATATA 1750 0.0 14.130285 1 ACATGGG 13710 0.0 13.98899 3 GTATAAA 1820 0.0 13.926482 1 TAACGGT 245 0.006873666 13.6938505 4 ATCGTTC 220 0.0069553936 13.669515 8 GTCCTAG 735 2.8376235E-10 13.457415 1 GTATATG 1565 0.0 13.430543 1 CCAGTAC 1045 0.0 13.376836 3 CTAGTAC 595 1.1732482E-8 13.156512 3 CTATACT 1150 0.0 13.128234 4 CTACACT 945 0.0 13.017611 4 >>END_MODULE