FastQCFastQC Report
Wed 25 May 2016
SRR1295162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682823
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT97460.36327405870607193No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73320.27329421284967365No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31030.11566174883695271No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC28080.10466586875093883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT41150.017.5127812-13
CATGGGG41550.017.0875114
GCGTGTA1700.00193381116.569869
GTATTAG11800.016.400661
AGTACTT47100.016.147712-13
ATAGTCG1750.002346412516.0970368
GTATCAA146100.014.7746691
GTACTTT50450.014.47057314-15
GTACATG176650.014.298251
TACGACT2000.005649202514.1056824
TACATGG179550.013.891362
CATGGGT21450.013.8097594
CCTAGAC9550.013.7851453
ACATGGG170700.013.7167883
CAACGCA159200.013.4677385
TCAACGC161200.013.4464854
ATCAACG160950.013.4376553
TAGACTG14350.013.4339845
TATCAAC158950.013.3831692
AACGCAG160200.013.3371366
Produced by FastQC (version 0.11.5)