##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295162_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2682823 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.278010140810633 33.0 33.0 33.0 27.0 33.0 2 29.99801552320075 33.0 33.0 33.0 27.0 33.0 3 30.79099180229184 33.0 33.0 33.0 27.0 33.0 4 35.03865741422375 37.0 37.0 37.0 33.0 37.0 5 35.340221848403715 37.0 37.0 37.0 33.0 37.0 6 35.059960720479886 37.0 37.0 37.0 33.0 37.0 7 34.94582683986234 37.0 37.0 37.0 33.0 37.0 8 34.93774058146959 37.0 37.0 37.0 33.0 37.0 9 34.91754469079772 37.0 37.0 37.0 33.0 37.0 10-11 34.883792184575725 37.0 37.0 37.0 33.0 37.0 12-13 34.785368061925816 37.0 37.0 37.0 30.0 37.0 14-15 36.185329594982605 37.0 37.0 40.0 27.0 40.0 16-17 36.17980239471631 37.0 37.0 40.0 27.0 40.0 18-19 36.212823022614614 38.5 37.0 40.0 27.0 40.0 20-21 36.215905782826525 38.5 37.0 40.0 27.0 40.0 22-23 36.183333004078165 37.0 37.0 40.0 27.0 40.0 24-25 36.149151472161975 37.0 37.0 40.0 27.0 40.0 26-27 36.11341803018686 37.0 37.0 40.0 27.0 40.0 28-29 36.061154425767185 37.0 37.0 40.0 27.0 40.0 30-31 35.96214714873102 37.0 37.0 40.0 27.0 40.0 32-33 35.91394903800959 37.0 37.0 40.0 27.0 40.0 34-35 35.823265642198535 37.0 37.0 40.0 27.0 40.0 36-37 35.80051889371755 37.0 37.0 40.0 27.0 40.0 38-39 35.731444638725705 37.0 37.0 40.0 27.0 40.0 40-41 35.6648185512052 37.0 37.0 40.0 27.0 40.0 42-43 35.56458681769166 37.0 37.0 40.0 27.0 40.0 44-45 35.458006547580666 37.0 37.0 40.0 27.0 40.0 46-47 35.33529569412518 37.0 35.0 40.0 27.0 40.0 48-49 35.21484365535855 37.0 33.0 40.0 27.0 40.0 50-51 35.11815352708695 37.0 33.0 40.0 27.0 40.0 52-53 35.02525716381588 37.0 33.0 40.0 27.0 40.0 54-55 34.901912835844925 37.0 33.0 40.0 24.5 40.0 56-57 34.77195196999578 37.0 33.0 40.0 22.0 40.0 58-59 34.64419307572658 37.0 33.0 40.0 22.0 40.0 60-61 34.489729102516264 37.0 33.0 40.0 22.0 40.0 62-63 34.309054492227034 37.0 33.0 40.0 22.0 40.0 64-65 34.13256446660849 37.0 33.0 40.0 22.0 40.0 66-67 33.90991336364718 37.0 33.0 40.0 22.0 40.0 68-69 33.700026986498926 37.0 33.0 37.0 22.0 40.0 70-71 33.46826980385959 37.0 33.0 37.0 22.0 40.0 72-73 33.25180490848632 37.0 33.0 37.0 22.0 40.0 74-75 33.01869150517943 37.0 33.0 37.0 22.0 40.0 76-77 32.777432577549845 37.0 33.0 37.0 22.0 37.0 78-79 32.54547336891029 37.0 33.0 37.0 22.0 37.0 80-81 32.34258186246353 37.0 33.0 37.0 22.0 37.0 82-83 32.142886056963135 35.0 33.0 37.0 22.0 37.0 84-85 31.966634586031205 33.0 33.0 37.0 22.0 37.0 86-87 31.9885545934264 33.0 33.0 37.0 22.0 37.0 88-89 31.997470574838516 33.0 33.0 37.0 22.0 37.0 90-91 32.00098366534058 33.0 33.0 37.0 22.0 37.0 92-93 31.962792178239116 33.0 33.0 37.0 22.0 37.0 94-95 31.990036800787827 33.0 33.0 37.0 22.0 37.0 96-97 31.945507213856448 33.0 33.0 37.0 22.0 37.0 98-99 31.867913760989822 33.0 33.0 37.0 22.0 37.0 100 31.337019624477648 33.0 27.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5363188806171841 1101 2 4.126960416075185 1101 3 2.291151423965541 1101 4 1.2042028687130184 1101 5 0.5398725626919543 1101 6 0.6032363815708166 1101 7 0.5976033933384528 1101 8 0.6492302461961614 1101 9 0.7624769498591988 1101 10-11 0.7517267368895375 1101 12-13 0.756882187448042 1101 14-15 1.0533089255308 1101 16-17 1.118076624290616 1101 18-19 1.0899076192674997 1101 20-21 1.0499320630867999 1101 22-23 1.0505217272477267 1101 24-25 1.0373395694792933 1101 26-27 1.0215325942278781 1101 28-29 1.0184633198957016 1101 30-31 0.872115019059045 1101 32-33 1.0451815471977497 1101 34-35 1.2080280545353688 1101 36-37 1.067373566149513 1101 38-39 1.110419234620693 1101 40-41 1.0633994505164779 1101 42-43 1.1398352490130534 1101 44-45 1.2002750385595249 1101 46-47 1.1591883124169016 1101 48-49 1.153528569332586 1101 50-51 1.062025188555971 1101 52-53 1.1385609427114005 1101 54-55 1.169257838104059 1101 56-57 1.0623610384149345 1101 58-59 1.0432420080293596 1101 60-61 1.0662229991893497 1101 62-63 1.1014532713589276 1101 64-65 1.17880438102641 1101 66-67 1.142977814975218 1101 68-69 1.0859577624259416 1101 70-71 1.0234329237245063 1101 72-73 0.9498786810986886 1101 74-75 0.8990864872137507 1101 76-77 0.9829002489358487 1101 78-79 0.970947081528962 1101 80-81 0.9261664980784374 1101 82-83 0.9138313101497744 1101 84-85 0.9758585056236448 1101 86-87 0.9758301708037322 1101 88-89 0.9576439084434369 1101 90-91 0.9899219903349845 1101 92-93 1.0178724050313228 1101 94-95 1.079991737605102 1101 96-97 0.9573002330192182 1101 98-99 0.9976450287160574 1101 100 1.1620084835291529 1102 1 0.47504439714691316 1102 2 4.040236729886608 1102 3 2.314646455866008 1102 4 1.0750720770509332 1102 5 0.4930423142272389 1102 6 0.44237906540780614 1102 7 0.46431571252514914 1102 8 0.5719889843747197 1102 9 0.5252801910792471 1102 10-11 0.5419613723339438 1102 12-13 0.6458166457690311 1102 14-15 0.844245611009427 1102 16-17 0.7907585992494646 1102 18-19 0.889609479717997 1102 20-21 0.8539487800345569 1102 22-23 0.8736235698715689 1102 24-25 0.9421563143216716 1102 26-27 0.864779449356881 1102 28-29 0.870381231427281 1102 30-31 0.8649064964607192 1102 32-33 0.8381759482459401 1102 34-35 1.002481694521613 1102 36-37 0.9508524423182152 1102 38-39 0.9408298497283809 1102 40-41 0.9484193499325144 1102 42-43 0.9442354167589855 1102 44-45 0.9169001999399242 1102 46-47 0.9227558479913611 1102 48-49 0.8489429865726095 1102 50-51 0.8512571245881233 1102 52-53 0.8772431704294519 1102 54-55 0.8564695140325966 1102 56-57 0.7852278797209706 1102 58-59 0.7722440048042287 1102 60-61 0.7196860625788872 1102 62-63 0.8546585386867562 1102 64-65 0.8471390235676637 1102 66-67 0.854386902325075 1102 68-69 0.7148362389646152 1102 70-71 0.7741823410417439 1102 72-73 0.7217619575697896 1102 74-75 0.7202901144431308 1102 76-77 0.677451267976366 1102 78-79 0.7192793850017267 1102 80-81 0.6401418277820881 1102 82-83 0.7807607610190601 1102 84-85 0.8017535352549494 1102 86-87 0.8144546438149476 1102 88-89 0.7701343479593916 1102 90-91 0.701197320442617 1102 92-93 0.835608515071609 1102 94-95 0.7124720472698094 1102 96-97 0.7598494738634187 1102 98-99 0.7794593040991984 1102 100 0.780717398517389 1103 1 0.4433789515755642 1103 2 3.8739664599468 1103 3 2.188797365494576 1103 4 0.942459773389146 1103 5 0.4545824997261505 1103 6 0.4580337160791714 1103 7 0.3361877928780075 1103 8 0.3622628286305556 1103 9 0.4096595852919336 1103 10-11 0.4656281894588261 1103 12-13 0.5466162670105064 1103 14-15 0.7170794453754112 1103 16-17 0.6589683929776768 1103 18-19 0.7237187942166301 1103 20-21 0.7088343796129379 1103 22-23 0.6119847561995471 1103 24-25 0.6415479828275537 1103 26-27 0.6887973346925449 1103 28-29 0.6871580024174335 1103 30-31 0.6581717817627535 1103 32-33 0.7793214170711522 1103 34-35 0.8748186405276854 1103 36-37 0.6396959282309993 1103 38-39 0.616843516825611 1103 40-41 0.6911249256427325 1103 42-43 0.7542880512725105 1103 44-45 0.8195386064430039 1103 46-47 0.6758850719134912 1103 48-49 0.6749461768513001 1103 50-51 0.6990537874840825 1103 52-53 0.7117911769050096 1103 54-55 0.6025193243168019 1103 56-57 0.6751663658502309 1103 58-59 0.6939349518542528 1103 60-61 0.6110691857395025 1103 62-63 0.7365444677213233 1103 64-65 0.6479409902997304 1103 66-67 0.6643767258939235 1103 68-69 0.5199339805246481 1103 70-71 0.6296455691134 1103 72-73 0.6246667594856135 1103 74-75 0.6898141823655948 1103 76-77 0.6155842363066881 1103 78-79 0.7055708889793664 1103 80-81 0.5488516307247266 1103 82-83 0.5898062747936308 1103 84-85 0.6370173278491471 1103 86-87 0.6558874293586641 1103 88-89 0.5680586799053344 1103 90-91 0.6223183544289199 1103 92-93 0.6352153103281744 1103 94-95 0.6191580709940006 1103 96-97 0.6836837966381424 1103 98-99 0.6480655239463786 1103 100 0.5524811483607657 1104 1 0.44590275459468387 1104 2 3.526683819923626 1104 3 2.135455672767627 1104 4 0.9445439301887504 1104 5 0.42071635949663033 1104 6 0.3978318227364568 1104 7 0.249021799273919 1104 8 0.31537562800898655 1104 9 0.33910272470380676 1104 10-11 0.3923551011599926 1104 12-13 0.4687729711884856 1104 14-15 0.5722870702855545 1104 16-17 0.566318356212328 1104 18-19 0.6065276612772053 1104 20-21 0.5902984687332946 1104 22-23 0.5241597171091144 1104 24-25 0.5066315416453264 1104 26-27 0.5617069413469338 1104 28-29 0.5764740846168053 1104 30-31 0.4940266778025588 1104 32-33 0.5712707415623939 1104 34-35 0.7196816217952815 1104 36-37 0.6619633795399196 1104 38-39 0.5866655669756824 1104 40-41 0.5587570671539552 1104 42-43 0.5217799885689374 1104 44-45 0.6079654480042223 1104 46-47 0.5965988936495208 1104 48-49 0.5170968963670646 1104 50-51 0.3836334491266271 1104 52-53 0.4696816337301897 1104 54-55 0.499575944041041 1104 56-57 0.5307441116016278 1104 58-59 0.5087063109950165 1104 60-61 0.5070480788458269 1104 62-63 0.5327250918990671 1104 64-65 0.5725834878680871 1104 66-67 0.4746671267233964 1104 68-69 0.5020315059358538 1104 70-71 0.45555612602363027 1104 72-73 0.4191130032442274 1104 74-75 0.4483389050027853 1104 76-77 0.4448482990519125 1104 78-79 0.49738155628253367 1104 80-81 0.4601548224073255 1104 82-83 0.5266492425612199 1104 84-85 0.5311735601082965 1104 86-87 0.5184062086280434 1104 88-89 0.46942902162930267 1104 90-91 0.4656639477218434 1104 92-93 0.5153961544198573 1104 94-95 0.5230193224980404 1104 96-97 0.4569186391671636 1104 98-99 0.45532992843011044 1104 100 0.4456611038354623 1105 1 0.42530310076266176 1105 2 3.7219809998149422 1105 3 2.1828236962258245 1105 4 1.0072299471297086 1105 5 0.3548915030886519 1105 6 0.4433118769823352 1105 7 0.4258582179824657 1105 8 0.40230053469834104 1105 9 0.35709505921037277 1105 10-11 0.43495292020503484 1105 12-13 0.41179824241786633 1105 14-15 0.5652682244366787 1105 16-17 0.5204251727239608 1105 18-19 0.5483051746211132 1105 20-21 0.46578170182618805 1105 22-23 0.4274648719177563 1105 24-25 0.5051942314091988 1105 26-27 0.44760784834571155 1105 28-29 0.43242396761588964 1105 30-31 0.43409574218206615 1105 32-33 0.5158413494352985 1105 34-35 0.5817513896306679 1105 36-37 0.49230326838905825 1105 38-39 0.5415286589906074 1105 40-41 0.4549588586699471 1105 42-43 0.5188019044829986 1105 44-45 0.5753802718259919 1105 46-47 0.535102950003342 1105 48-49 0.47423298486229726 1105 50-51 0.4469206670190715 1105 52-53 0.22710143129927474 1105 54-55 0.3839298826687525 1105 56-57 0.45814586347267294 1105 58-59 0.48781424554218944 1105 60-61 0.5005417163170662 1105 62-63 0.48149543410255546 1105 64-65 0.46298134534937674 1105 66-67 0.4509799119735476 1105 68-69 0.42360641915211517 1105 70-71 0.44320730255963525 1105 72-73 0.494602551727084 1105 74-75 0.46096515236784796 1105 76-77 0.37407166321482066 1105 78-79 0.4263115116578007 1105 80-81 0.38353219022531704 1105 82-83 0.4973746905006493 1105 84-85 0.4843282031588281 1105 86-87 0.43374369218989983 1105 88-89 0.45290442605600134 1105 90-91 0.480480275633802 1105 92-93 0.4758305686417508 1105 94-95 0.5260622526455982 1105 96-97 0.39387161761386125 1105 98-99 0.44619820692128087 1105 100 0.4359393919477803 1106 1 0.49838439873076723 1106 2 3.8513470882248484 1106 3 2.1920670538384535 1106 4 0.9031656857559511 1106 5 0.39146653696243305 1106 6 0.256929804413474 1106 7 0.1941801198090829 1106 8 0.24791347609825465 1106 9 0.2548527969957348 1106 10-11 0.21893053936030782 1106 12-13 0.21227727533876362 1106 14-15 0.25557407069754845 1106 16-17 0.2683932494427168 1106 18-19 0.37615417977040266 1106 20-21 0.27063721566028676 1106 22-23 0.23515405029222336 1106 24-25 0.16733629004040296 1106 26-27 0.22714520935802085 1106 28-29 0.15875305989924726 1106 30-31 0.21808167237703913 1106 32-33 0.35533681747424595 1106 34-35 0.3676947844874192 1106 36-37 0.2518179718182765 1106 38-39 0.2646346678574929 1106 40-41 0.3480327255907909 1106 42-43 0.36796361538978317 1106 44-45 0.3283008837674046 1106 46-47 0.2605430277034131 1106 48-49 0.21979137858276943 1106 50-51 0.09648670037759643 1106 52-53 0.07518537368910927 1106 54-55 0.19208181262253987 1106 56-57 0.21042683646579263 1106 58-59 0.14401847371326681 1106 60-61 0.20793852683335956 1106 62-63 0.22473913660110867 1106 64-65 0.18924593448328153 1106 66-67 0.13976202317910236 1106 68-69 0.05037841133351861 1106 70-71 0.08836067753804144 1106 72-73 0.1570267264526919 1106 74-75 0.050960230928652095 1106 76-77 0.10861416640516097 1106 78-79 0.11683757824863505 1106 80-81 0.16163806724147634 1106 82-83 0.19386463177226432 1106 84-85 0.18095795063655729 1106 86-87 0.2871015151893488 1106 88-89 0.22364073676621388 1106 90-91 0.2653363613148443 1106 92-93 0.28477549918901346 1106 94-95 0.2423232684768699 1106 96-97 0.2189020692040522 1106 98-99 0.22508564759215943 1106 100 0.28557437286201903 1107 1 0.34866399966204753 1107 2 3.435841755161018 1107 3 2.0051475436778787 1107 4 0.8730207671820338 1107 5 0.22328394404445362 1107 6 0.18730924747359268 1107 7 0.059706501675542256 1107 8 0.0378166521940102 1107 9 0.04592708185447236 1107 10-11 0.07616488629177809 1107 12-13 0.09488053622638404 1107 14-15 0.0893636549158785 1107 16-17 0.041854537589465224 1107 18-19 0.10971563511721172 1107 20-21 0.12276190439944656 1107 22-23 0.00980693315521819 1107 24-25 0.1518544203000758 1107 26-27 0.12472430460955763 1107 28-29 0.1327743581778691 1107 30-31 0.1238361178656362 1107 32-33 0.20005320355927836 1107 34-35 0.3959696764710472 1107 36-37 0.16585920583919034 1107 38-39 0.14242129022433403 1107 40-41 0.10115889822424151 1107 42-43 0.2147763287783846 1107 44-45 0.18898036844898058 1107 46-47 0.20441721773305943 1107 48-49 0.06086618808177491 1107 50-51 0.029306337900841584 1107 52-53 -0.0020128256749600837 1107 54-55 0.1353274630387702 1107 56-57 0.12333143808410796 1107 58-59 0.18128411271791123 1107 60-61 0.25927495900268127 1107 62-63 0.3741665306940902 1107 64-65 0.14508192258943353 1107 66-67 0.13727693302929822 1107 68-69 0.1628637827610433 1107 70-71 0.14737156810004848 1107 72-73 0.05759803472005842 1107 74-75 0.11672299189199009 1107 76-77 0.18276248641150517 1107 78-79 0.1263288791104884 1107 80-81 0.09946170011344435 1107 82-83 0.13741682200136296 1107 84-85 0.20441638044907862 1107 86-87 0.22983163673865192 1107 88-89 0.11489183274267134 1107 90-91 0.17669624269970896 1107 92-93 0.24541025571610575 1107 94-95 0.1117321646651952 1107 96-97 0.046801618551288726 1107 98-99 0.1443054778303292 1107 100 0.20853775388255613 1108 1 0.46769163084547216 1108 2 3.6100574211932432 1108 3 2.0551907882013083 1108 4 0.9729196161046119 1108 5 0.3474280750163885 1108 6 0.29135419281592334 1108 7 0.17125608853444163 1108 8 0.12711381949671363 1108 9 0.14525536115687032 1108 10-11 0.18114176979922547 1108 12-13 0.09812815263916264 1108 14-15 0.060737937959515875 1108 16-17 0.041035776169600524 1108 18-19 0.11797940603965174 1108 20-21 0.08404164800134595 1108 22-23 0.019475027058547312 1108 24-25 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64-65 -0.13263024888036057 2210 66-67 0.18937894956883383 2210 68-69 0.3524474823191852 2210 70-71 0.3032932993472457 2210 72-73 0.2876354576808069 2210 74-75 -0.30397008092771216 2210 76-77 0.05049712681386609 2210 78-79 0.2362705330638164 2210 80-81 0.32468583352365243 2210 82-83 0.35664007367809347 2210 84-85 0.41018607792815587 2210 86-87 0.2798311257940469 2210 88-89 0.4233262613460482 2210 90-91 0.40379866206260573 2210 92-93 0.45303881559825854 2210 94-95 0.4101835394066278 2210 96-97 0.4188557119313714 2210 98-99 0.30555389270601907 2210 100 0.22128706566696366 2211 1 0.46654658937289284 2211 2 3.8602197616666736 2211 3 2.1787686856540773 2211 4 0.9089293305585358 2211 5 0.4080351850962174 2211 6 0.2124264191748253 2211 7 0.19123695400396912 2211 8 0.12368302415826093 2211 9 0.10576982893986298 2211 10-11 0.1707530617696591 2211 12-13 0.07481281384542626 2211 14-15 0.023853100126828508 2211 16-17 0.0660665126325668 2211 18-19 0.03369331450560509 2211 20-21 0.09631025871322407 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-0.13278216606945747 2211 86-87 0.008205820107772155 2211 88-89 -0.07122175189690694 2211 90-91 -0.08087352089719957 2211 92-93 -0.032636302754848856 2211 94-95 0.09346332588172501 2211 96-97 0.05809549238034606 2211 98-99 0.08628820229534995 2211 100 -0.10514545714200452 2212 1 0.5422525903144724 2212 2 4.085636964571339 2212 3 2.1820221403374944 2212 4 1.1706758918161384 2212 5 0.6941298385863917 2212 6 0.4299122237018551 2212 7 0.36667418944956154 2212 8 0.44177809424027714 2212 9 0.24918326763975784 2212 10-11 0.32159862628878244 2212 12-13 0.34714654641259557 2212 14-15 0.6724701108891367 2212 16-17 0.6377664304832109 2212 18-19 0.6557447317148828 2212 20-21 0.5012570741768556 2212 22-23 0.4509193913881049 2212 24-25 0.6216146319385984 2212 26-27 0.4771715906796672 2212 28-29 0.44773772393047295 2212 30-31 0.33094637317432074 2212 32-33 0.40146895526864057 2212 34-35 0.49722048623368664 2212 36-37 0.5699470047579496 2212 38-39 0.4793729707399237 2212 40-41 0.5542088757573751 2212 42-43 0.5709142217251468 2212 44-45 0.5830112081551135 2212 46-47 0.6804063917227765 2212 48-49 0.40963938612490125 2212 50-51 0.4109736815527114 2212 52-53 0.41758839203546927 2212 54-55 0.18315939483810695 2212 56-57 0.3417282590684678 2212 58-59 0.543662186040649 2212 60-61 0.4331914488210913 2212 62-63 0.4817157066315545 2212 64-65 0.45479895099630596 2212 66-67 0.48176090080768574 2212 68-69 0.4238764005734197 2212 70-71 0.6086238941451612 2212 72-73 0.7162538811292691 2212 74-75 0.49579928000785856 2212 76-77 0.43363813953785524 2212 78-79 0.4481163172649616 2212 80-81 0.3924853830895998 2212 82-83 0.47286541047181885 2212 84-85 0.6216918920557255 2212 86-87 0.5593773252264285 2212 88-89 0.5687802035341392 2212 90-91 0.43667711042008506 2212 92-93 0.7631044708519319 2212 94-95 0.7322472815699577 2212 96-97 0.5901732760267819 2212 98-99 0.6930264689120023 2212 100 0.5987540337020079 2213 1 -0.7253539625523615 2213 2 -25.7656265733348 2213 3 -14.734605697530371 2213 4 -6.2929530165625955 2213 5 -2.3581368403079637 2213 6 -1.6072442031550906 2213 7 -1.4610035426899302 2213 8 -1.3240967706231572 2213 9 -1.4191181473040118 2213 10-11 -1.2296298157916397 2213 12-13 -1.2709528470821567 2213 14-15 -1.4216369648878668 2213 16-17 -1.4456732778863497 2213 18-19 -1.5982804420447252 2213 20-21 -1.5185498458734443 2213 22-23 -1.5359434826954796 2213 24-25 -1.4835446308021574 2213 26-27 -1.5693384909930828 2213 28-29 -1.4708207370734812 2213 30-31 -1.4463281096913363 2213 32-33 -1.4604257960536273 2213 34-35 -1.3361699292550355 2213 36-37 -1.574774071608097 2213 38-39 -1.568233370493786 2213 40-41 -1.5484081441308533 2213 42-43 -1.6089143201755576 2213 44-45 -1.4963411592258709 2213 46-47 -1.6708809549093502 2213 48-49 -1.5663178569304392 2213 50-51 -1.41514219816208 2213 52-53 -1.5594959737441627 2213 54-55 -1.4991607743272937 2213 56-57 -1.3786163854672466 2213 58-59 -1.3339101704412286 2213 60-61 -1.3241988679055083 2213 62-63 -1.2800878660882304 2213 64-65 -1.3667370116433943 2213 66-67 -1.410977261405577 2213 68-69 -1.3857119906445092 2213 70-71 -1.4716048107130533 2213 72-73 -1.4954303834191833 2213 74-75 -1.5476079074056202 2213 76-77 -1.3852345966491164 2213 78-79 -1.4167954405420957 2213 80-81 -1.289589468017681 2213 82-83 -1.4522642589043357 2213 84-85 -1.4503810014451943 2213 86-87 -1.2247812303069807 2213 88-89 -1.2531031901440244 2213 90-91 -1.4104657542810912 2213 92-93 -1.3547873458244055 2213 94-95 -1.4960253159973185 2213 96-97 -1.5888228422690815 2213 98-99 -1.2394253536900663 2213 100 -1.2685548219095324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 30.0 5 40.0 6 40.0 7 61.0 8 49.0 9 67.0 10 429.0 11 2044.0 12 6060.0 13 11156.0 14 14583.0 15 16517.0 16 17333.0 17 17753.0 18 18019.0 19 19189.0 20 20771.0 21 23333.0 22 25989.0 23 27180.0 24 27825.0 25 28523.0 26 30755.0 27 34875.0 28 40124.0 29 48135.0 30 57989.0 31 71014.0 32 90336.0 33 119141.0 34 162490.0 35 230484.0 36 348856.0 37 585587.0 38 581028.0 39 5008.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.19475273975764 20.931361861162596 10.225113641353031 30.648771757726728 2 12.364907094760593 22.76809264187359 36.40670757353605 28.460292689829757 3 15.140991410913058 27.55552639887164 28.779051022001823 28.524431168213482 4 10.341144948021972 18.761667471834052 37.35555446211837 33.54163311802561 5 11.633316258641264 38.55701926652499 35.02543282850986 14.784231646323889 6 29.862297830823476 39.58954146766449 17.521689154564925 13.026471546947107 7 25.60801176498664 33.58942463726613 23.247594203417336 17.5549693943299 8 24.399630978146565 37.27165467668823 20.65754205193453 17.671172293230672 9 24.569111299629903 16.970457448097772 22.181841911456246 36.27858934081608 10-11 23.04811219983596 28.05372528670932 28.657395486671692 20.24076702678303 12-13 23.64959121461 25.585994089625146 29.476195359330283 21.288219336434572 14-15 21.515976255676772 26.646788369033036 26.630417860038865 25.206817515251327 16-17 20.593627098673423 30.49920718594356 27.65490169758222 21.252264017800794 18-19 20.57370518225377 29.300160287421186 29.80307651925369 20.323058011071353 20-21 21.783186702669553 28.531248223235284 28.985894934834093 20.69967013926107 22-23 20.804872565478874 28.662654290263855 29.169365010303107 21.363108133954167 24-25 21.054632029112426 28.331005099744957 28.99128021530848 21.623082655834136 26-27 21.065766505466485 28.404863778235274 28.978343754339498 21.551025961958743 28-29 20.61371394315011 28.25686017778825 29.675721360687053 21.453704518374582 30-31 21.55698624539363 28.58122346351219 28.778732482376117 21.08305780871806 32-33 20.320647116522387 28.77418630990421 29.528996547814806 21.376170025758597 34-35 20.577803100236867 28.15172824851397 29.596524883882296 21.67394376736687 36-37 21.344854032726264 28.649526644257712 28.855810780713142 21.14980854230288 38-39 21.023330159225406 28.51634963795972 29.22305926970915 21.237260933105723 40-41 21.219286261455622 28.395271812974933 28.850272213700336 21.535169711869116 42-43 20.913857965508296 28.612481249645867 29.15517912366138 21.318481661184453 44-45 21.435197464250628 27.92964436174688 28.96229944126956 21.672858732732934 46-47 21.305072038825323 28.72985971749641 28.8542458487317 21.110822394946563 48-49 21.181959199241348 28.512043499731465 28.984112076533158 21.32188522449403 50-51 21.447850368265165 28.469970138747914 28.45330495405237 21.62887453893455 52-53 20.750867188984095 28.852230847972592 28.48833491698904 21.908567046054273 54-55 21.28267171232302 28.58909280714346 28.210910921174047 21.917324559359475 56-57 21.444138540371572 28.391866224027407 28.30653203573672 21.8574631998643 58-59 20.917750805939768 28.010207172421246 29.04740202670902 22.024639994929966 60-61 21.6259268213079 28.456431406882082 28.21862982823598 21.699011943574035 62-63 20.715361002230143 28.826158373776906 28.66363224917963 21.794848374813316 64-65 20.7635875045996 28.180452663927475 28.824061001402157 22.23189883007077 66-67 21.260743009412746 28.41090607107606 28.478665904873395 21.849685014637803 68-69 21.34169325982325 28.35000136088358 28.49727506365393 21.811030315639236 70-71 21.42614527589191 28.28534761974182 28.175675398561488 22.112831705804776 72-73 21.052979883072247 28.660088159992902 28.582725398735125 21.704206558199726 74-75 21.603246483063675 27.90884524585372 28.307646363160494 22.180261907922112 76-77 21.25071882266866 28.49475504107891 28.539007488804074 21.715518647448352 78-79 21.25968268690752 28.41657161912363 28.726756844867683 21.59698884910116 80-81 21.400299165412378 28.303601634692004 28.290833515845982 22.00526568404964 82-83 20.704513358012093 28.707541013794994 28.41426248791519 22.17368314027772 84-85 21.014932528323044 28.16580448404549 28.23762093513854 22.581642052492924 86-87 21.25143191787343 28.41774985452497 28.17535360403431 22.15546462356729 88-89 20.923856469662454 28.036163838807855 28.688323691966577 22.351655999563114 90-91 21.355576492778987 28.17487365441839 28.24221447482827 22.227335377974352 92-93 20.549371635759908 28.669098651573528 28.52169226040963 22.259837452256935 94-95 20.581500086384096 27.984961898742927 28.734127271822512 22.69941074305046 96-97 21.068655682746442 28.26885562307 28.38403377366301 22.278454920520552 98-99 20.899326880265125 28.051035361663246 28.481126014865403 22.56851174320623 100 20.968065134721538 28.005631635372136 28.334641236989533 22.6916619929168 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 7.5 2 9.5 3 14.0 4 15.5 5 29.0 6 68.0 7 138.5 8 232.5 9 281.5 10 269.0 11 266.0 12 292.0 13 340.0 14 422.5 15 536.0 16 650.5 17 796.5 18 975.5 19 1260.5 20 1690.5 21 2350.0 22 3273.0 23 4758.0 24 6945.0 25 9974.0 26 14375.5 27 20507.0 28 28259.0 29 36650.5 30 44880.0 31 53972.5 32 63561.0 33 72332.0 34 80802.0 35 88516.0 36 95296.5 37 101136.0 38 106099.5 39 109538.5 40 110791.5 41 110566.0 42 109162.5 43 106697.0 44 102682.0 45 97811.5 46 92901.5 47 88659.0 48 84938.5 49 84702.5 50 97660.5 51 94054.0 52 71486.5 53 60887.0 54 56081.0 55 51947.0 56 47708.5 57 43169.0 58 38860.0 59 34599.5 60 29687.0 61 24863.5 62 20706.5 63 16705.0 64 12914.5 65 10230.0 66 8021.5 67 6016.5 68 4540.0 69 3484.0 70 2603.5 71 1896.0 72 1420.5 73 1011.0 74 708.5 75 487.5 76 291.0 77 160.0 78 88.0 79 39.5 80 23.5 81 14.0 82 8.0 83 4.0 84 2.0 85 2.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.6739169896784096 2 5.174884813496828 3 0.0 4 0.00562839963724778 5 0.16381997619671518 6 0.05699220559835666 7 0.03604412218025565 8 0.042455279382948484 9 0.04092703842184147 10-11 0.04262301314697243 12-13 0.04092703842184147 14-15 0.04366668990089916 16-17 0.034310873285341596 18-19 0.028552014053852975 20-21 0.023501364048243212 22-23 0.02318453360508688 24-25 0.019829858324608072 26-27 0.012691854811144827 28-29 0.019792584154824974 30-31 0.024861871245326288 32-33 0.0010995880086013875 34-35 0.003447860704936554 36-37 0.004305166609947805 38-39 0.006690713476066069 40-41 0.007007543919222401 42-43 0.007715753145101261 44-45 0.008330776946522376 46-47 0.016773376402394045 48-49 0.025775088405012185 50-51 0.02178675223822071 52-53 0.027489700215034687 54-55 0.024153662019447426 56-57 0.015953344667165893 58-59 0.014741934149215211 60-61 0.012468209792446242 62-63 0.018077972344802472 64-65 0.019531664966343287 66-67 0.02070580131451087 68-69 0.027657433979058628 70-71 0.027582885639492433 72-73 0.02437730703814601 74-75 0.0245636778870615 76-77 0.01809660942969402 78-79 0.014667385809649016 80-81 0.013437338206806785 82-83 0.008181680267389984 84-85 0.010809509237098384 86-87 0.008647607389678708 88-89 0.007398922701944929 90-91 0.007473471041511125 92-93 0.0038578765725506307 94-95 0.0013977813668661703 96-97 8.386688201197023E-4 98-99 6.15023801421115E-4 100 0.11040609089753592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2682823.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.33113254827879 #Duplication Level Percentage of deduplicated Percentage of total 1 88.23717123262534 71.76429069205824 2 8.633817142467088 14.043962528231848 3 1.830910782852849 4.467301426928339 4 0.5570530192476841 1.8122301177940918 5 0.24552137593962636 0.9984265784990771 6 0.14012416990345603 0.6837874461381311 7 0.08022277460966705 0.45672263806186675 8 0.05567581155762612 0.3622541447621026 9 0.04217595127855277 0.30871960954071515 >10 0.1671132390111951 2.2994892379434235 >50 0.005876156561454045 0.32334198655253266 >100 0.0029121937703007394 0.44032478809354414 >500 7.820823542021317E-4 0.472791873347046 >1k 5.520581323573377E-4 0.9273620275860567 >5k 9.200968872622294E-5 0.6389949044629685 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9746 0.36327405870607193 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7332 0.27329421284967365 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3103 0.11566174883695271 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2808 0.10466586875093883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.7274169783097875E-5 0.0 6 0.0 0.0 0.0 3.7274169783097875E-5 0.0 7 0.0 0.0 0.0 3.7274169783097875E-5 0.0 8 0.0 0.0 0.0 3.7274169783097875E-5 0.0 9 0.0 0.0 0.0 7.454833956619575E-5 0.0 10-11 0.0 0.0 0.0 1.1182250934929362E-4 0.0 12-13 0.0 0.0 0.0 2.2364501869858727E-4 0.0 14-15 0.0 5.5911254674646816E-5 0.0 2.6091918848168515E-4 0.0 16-17 0.0 1.1182250934929363E-4 0.0 3.16830443156332E-4 0.0 18-19 0.0 1.1182250934929363E-4 0.0 4.8456420718027244E-4 0.0 20-21 0.0 1.1182250934929363E-4 0.0 6.33660886312664E-4 0.0 22-23 0.0 1.1182250934929363E-4 0.0 6.895721409873108E-4 0.0 24-25 0.0 1.1182250934929363E-4 0.0 8.759429899028001E-4 0.0 26-27 0.0 1.1182250934929363E-4 0.0 9.87765499252094E-4 0.0 28-29 0.0 1.1182250934929363E-4 0.0 0.0016214263855647577 0.0 30-31 3.7274169783097875E-5 1.1182250934929363E-4 0.0 0.0037087798934182393 0.0 32-33 7.454833956619575E-5 1.1182250934929363E-4 0.0 0.009616735804039253 0.0 34-35 1.1182250934929363E-4 1.3045959424084257E-4 0.0 0.01912164909872921 0.0 36-37 1.1182250934929363E-4 1.6773376402394046E-4 0.0 0.03418041369110075 0.0 38-39 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.05684310891922427 0.0 40-41 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.08574922758601666 0.0 42-43 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.1145062495736767 0.0 44-45 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.1484443811611873 0.0 46-47 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.18890549246074007 0.0 48-49 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.23240444859761528 0.0 50-51 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.28018993425954675 0.0 52-53 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.3309014422494514 0.0 54-55 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.3877445511686757 0.0 56-57 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.4545398634199871 0.0 58-59 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.530653718117073 3.7274169783097875E-5 60-61 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.6119300453291179 3.7274169783097875E-5 62-63 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.6967660557554487 3.7274169783097875E-5 64-65 1.1182250934929363E-4 1.8637084891548938E-4 0.0 0.7895601014304708 3.7274169783097875E-5 66-67 1.1182250934929363E-4 2.2364501869858727E-4 0.0 0.8987175076402729 3.7274169783097875E-5 68-69 1.1182250934929363E-4 2.2364501869858727E-4 0.0 1.0245923789977944 5.591125467464681E-5 70-71 1.1182250934929363E-4 2.2364501869858727E-4 0.0 1.174807283223679 7.454833956619575E-5 72-73 1.1182250934929363E-4 2.2364501869858727E-4 0.0 1.3236244060826972 7.454833956619575E-5 74-75 1.1182250934929363E-4 2.2364501869858727E-4 0.0 1.4780140173242886 7.454833956619575E-5 76-77 1.1182250934929363E-4 2.2364501869858727E-4 0.0 1.6515252776646094 7.454833956619575E-5 78-79 1.1182250934929363E-4 2.2364501869858727E-4 0.0 1.8453695976216098 7.454833956619575E-5 80-81 1.3045959424084257E-4 2.2364501869858727E-4 0.0 2.0623798140988057 7.454833956619575E-5 82-83 1.490966791323915E-4 2.2364501869858727E-4 0.0 2.2945978918475056 7.454833956619575E-5 84-85 1.490966791323915E-4 2.2364501869858727E-4 0.0 2.5336930539211866 7.454833956619575E-5 86-87 1.490966791323915E-4 2.2364501869858727E-4 0.0 2.795898201260389 7.454833956619575E-5 88 1.490966791323915E-4 2.2364501869858727E-4 0.0 3.0157412546411 7.454833956619575E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 4115 0.0 17.51278 12-13 CATGGGG 4155 0.0 17.087511 4 GCGTGTA 170 0.001933811 16.56986 9 GTATTAG 1180 0.0 16.40066 1 AGTACTT 4710 0.0 16.1477 12-13 ATAGTCG 175 0.0023464125 16.097036 8 GTATCAA 14610 0.0 14.774669 1 GTACTTT 5045 0.0 14.470573 14-15 GTACATG 17665 0.0 14.29825 1 TACGACT 200 0.0056492025 14.105682 4 TACATGG 17955 0.0 13.89136 2 CATGGGT 2145 0.0 13.809759 4 CCTAGAC 955 0.0 13.785145 3 ACATGGG 17070 0.0 13.716788 3 CAACGCA 15920 0.0 13.467738 5 TCAACGC 16120 0.0 13.446485 4 ATCAACG 16095 0.0 13.437655 3 TAGACTG 1435 0.0 13.433984 5 TATCAAC 15895 0.0 13.383169 2 AACGCAG 16020 0.0 13.337136 6 >>END_MODULE