FastQCFastQC Report
Wed 25 May 2016
SRR1295161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782992
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22480.12608020675359172No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18700.10487988729057673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1058.182249E-426.7964443
TAATCCG1905.1912793E-621.101325
GTATAGG4501.9985237E-816.5849321
TATACGT2403.0474455E-416.410974
CATGGGG23150.016.2843844
ACTATAC6650.016.0778683
TAAACCG1950.003123876615.4201965
TACAGCG2305.518625E-415.1789437
GTACATG95500.014.9134151
GTGTAGC4655.2292125E-714.71244051
GTCTTGC9300.014.71244051
TAGACAG8700.014.4010455
TACATGG96200.014.0267652
GTACATC4951.059303E-613.8207771
TACATCG2750.007307169513.5672372
GTCCTAC3702.4678314E-413.4472441
CTACACC5454.8641596E-813.4212934
GCCCTAT4204.820311E-513.3271781
GTATATG11250.013.2679461
CCTATAC4301.0566768E-513.0866363
Produced by FastQC (version 0.11.5)