FastQCFastQC Report
Wed 25 May 2016
SRR1295161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782992
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT66680.3739781221676822No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48920.27437027199224673No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22060.12372461570214562No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19030.1067307088309987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1200.00506174919.5856445
GAGTACT30700.019.4212912-13
GTACTAG3656.002665E-1119.0855771
CACGGGT2051.7401368E-518.343634
ACCGATA1750.002347158716.0958428
AGTACTT35000.015.6268212-13
GTACTTT38700.015.52764614-15
GTCTAGC3654.216472E-714.99581051
CATGGGG28950.014.9378594
TTGCGAC950.00406403114.82324858-59
CCTACAC6350.014.8048973
GCGTGCC1950.00482328214.4453939
CTACACT7850.014.3711224
CGAGTAC2308.64696E-414.3060363
TATACAG11500.014.3060365
CTAGTAC3951.2558849E-714.2801663
GTATAGG5601.1823431E-1014.2168061
TACACTG13000.014.1016655
GTACATG128700.014.034541
CTAGCAC7750.013.9500343
Produced by FastQC (version 0.11.5)