FastQCFastQC Report
Wed 25 May 2016
SRR1295160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701038
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7800.019.6251771
TAGACCG2401.6583726E-619.0132265
GTATACG2551.8596233E-417.5087361
TAGTGCG1800.001649866216.9680549
GTACATG127100.016.8612841
TACATGG130700.016.4418432
CATGGGT22350.016.2126524
TATATCG3151.2365908E-616.0958525
TAGTACG2150.002391621216.0510654
GTATAAG16100.015.8464161
ACATGGG132950.015.790493
CCGCGCT1950.002816084815.6628219
GTATAGG9950.015.3845611
GTATTAG15400.015.3242041
GTCTAGG9800.014.9692031
GTCTCGC4704.4418994E-714.9276611
GTATCAA92800.014.6395791
GTGTAAG14200.014.3733691
CTATCCG2150.005390513714.2058139
GAGTACG3159.278221E-414.1737391
Produced by FastQC (version 0.11.5)