##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3701038 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.749440562350348 27.0 22.0 33.0 2.0 33.0 2 21.53333227056842 27.0 2.0 33.0 2.0 33.0 3 24.15246938831755 27.0 15.0 33.0 6.0 33.0 4 23.762990004425784 33.0 2.0 37.0 2.0 37.0 5 25.984701048732816 33.0 15.0 37.0 2.0 37.0 6 27.04581255312699 33.0 15.0 37.0 2.0 37.0 7 26.824753758269978 33.0 15.0 37.0 2.0 37.0 8 27.49956985040413 33.0 15.0 37.0 2.0 37.0 9 28.625245404127167 33.0 22.0 37.0 6.0 37.0 10-11 28.129576756574778 33.0 18.5 37.0 4.0 37.0 12-13 26.889864816302886 33.0 15.0 37.0 2.0 37.0 14-15 28.76430571639632 33.0 18.5 37.0 4.0 40.0 16-17 29.14885351082588 33.0 22.0 38.5 4.0 40.0 18-19 29.371342174816903 33.0 22.0 40.0 6.0 40.0 20-21 28.872480233923564 33.0 22.0 40.0 4.0 40.0 22-23 29.70284660681679 33.0 27.0 40.0 6.0 40.0 24-25 29.930276722368156 33.0 27.0 40.0 6.0 40.0 26-27 28.324659325302797 33.0 18.5 38.5 2.0 40.0 28-29 29.091094714509822 33.0 22.0 40.0 4.0 40.0 30-31 29.909910679112183 33.0 27.0 40.0 10.5 40.0 32-33 28.617829376515452 33.0 22.0 37.0 2.0 40.0 34-35 28.26927688934834 33.0 22.0 37.0 6.0 40.0 36-37 28.58447738175074 33.0 22.0 37.0 6.0 40.0 38-39 28.759050298862103 33.0 22.0 37.0 6.0 40.0 40-41 27.757228377552458 33.0 15.0 37.0 4.0 40.0 42-43 28.413314453945084 33.0 22.0 37.0 6.0 40.0 44-45 28.336391034082872 33.0 22.0 37.0 6.0 40.0 46-47 28.08177449137242 33.0 22.0 37.0 6.0 40.0 48-49 28.15729951975635 33.0 22.0 37.0 6.0 40.0 50-51 27.93195733197011 33.0 22.0 37.0 4.0 40.0 52-53 28.23866020829832 33.0 22.0 37.0 6.0 40.0 54-55 28.28927411715308 33.0 22.0 37.0 6.0 40.0 56-57 28.278485792364197 33.0 22.0 37.0 6.0 40.0 58-59 28.202482925060483 33.0 22.0 37.0 6.0 40.0 60-61 28.124071138961554 33.0 22.0 37.0 6.0 40.0 62-63 28.219486668334667 33.0 22.0 37.0 6.0 40.0 64-65 27.76275912325137 33.0 22.0 37.0 6.0 40.0 66-67 27.997895860566686 33.0 22.0 37.0 10.5 40.0 68-69 27.41911269216906 33.0 22.0 37.0 6.0 40.0 70-71 27.674532387940896 33.0 22.0 37.0 10.5 37.0 72-73 27.80606427169891 33.0 22.0 37.0 10.5 37.0 74-75 27.955444796838076 33.0 22.0 37.0 15.0 37.0 76-77 27.736027973773844 33.0 22.0 37.0 10.5 37.0 78-79 27.221528122650998 30.0 22.0 37.0 10.5 37.0 80-81 27.062599330241948 27.0 22.0 35.0 6.0 37.0 82-83 27.17022791984303 27.0 22.0 33.0 10.5 37.0 84-85 27.112755664762155 27.0 22.0 33.0 10.5 37.0 86-87 27.001380558643277 27.0 22.0 33.0 15.0 37.0 88-89 26.947112674876614 27.0 22.0 33.0 15.0 37.0 90-91 27.107423917290234 27.0 22.0 33.0 15.0 37.0 92-93 26.99706122984957 27.0 22.0 33.0 15.0 37.0 94-95 26.826342501752208 27.0 22.0 33.0 15.0 37.0 96-97 26.917612977764616 27.0 22.0 33.0 15.0 37.0 98-99 27.00735010016109 27.0 22.0 33.0 15.0 37.0 100 26.631310729584513 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.230301398774241 1101 2 11.195159263637837 1101 3 8.455124449812793 1101 4 12.796194958699814 1101 5 10.77489174030497 1101 6 9.926065685491455 1101 7 9.39877831903868 1101 8 8.894109745154523 1101 9 7.289741968263172 1101 10-11 7.70338683369264 1101 12-13 9.065406642214064 1101 14-15 8.010097577282558 1101 16-17 8.130866674272351 1101 18-19 8.085468056431356 1101 20-21 7.15704178881883 1101 22-23 7.344599570829434 1101 24-25 7.18988432586962 1101 26-27 8.40088178489555 1101 28-29 8.180240667455035 1101 30-31 5.544695700923427 1101 32-33 7.166070015493627 1101 34-35 8.672479232597968 1101 36-37 8.13879054089547 1101 38-39 8.183844120653603 1101 40-41 9.051817757630651 1101 42-43 8.561818665892378 1101 44-45 8.427348068503793 1101 46-47 7.3195895662818415 1101 48-49 5.3309872702769106 1101 50-51 6.719082129509115 1101 52-53 5.908699923578482 1101 54-55 4.120392934384352 1101 56-57 6.170524615706437 1101 58-59 6.21890338084415 1101 60-61 6.70165468915587 1101 62-63 6.197146426499398 1101 64-65 6.721545115307368 1101 66-67 5.874491847259449 1101 68-69 6.842648902106415 1101 70-71 6.360491465749863 1101 72-73 5.563967055258214 1101 74-75 4.91648115254349 1101 76-77 5.076721291987955 1101 78-79 5.148628583786717 1101 80-81 5.026671091780436 1101 82-83 4.484347052678139 1101 84-85 4.239313784095376 1101 86-87 4.108217822241659 1101 88-89 4.026389207876651 1101 90-91 3.8131772441290295 1101 92-93 3.7513675382861678 1101 94-95 3.675606371246797 1101 96-97 3.7433654458167815 1101 98-99 3.479636707423591 1101 100 3.789987320569974 1102 1 6.766295753196502 1102 2 10.474845130742423 1102 3 8.219119823297103 1102 4 12.62632608978506 1102 5 10.677518605870429 1102 6 9.599978796739105 1102 7 6.840218713251446 1102 8 7.8741367486540526 1102 9 6.5290103511746125 1102 10-11 7.4907426229965 1102 12-13 9.134937239674478 1102 14-15 6.492481979367831 1102 16-17 7.5700265050052415 1102 18-19 7.5197688006446235 1102 20-21 7.1428438948255675 1102 22-23 6.707897840005597 1102 24-25 7.022090980193326 1102 26-27 8.671689836036531 1102 28-29 7.836480136096913 1102 30-31 4.444519132224535 1102 32-33 4.747911279921585 1102 34-35 5.41392618480894 1102 36-37 5.543708498310632 1102 38-39 4.755668917793145 1102 40-41 6.581634760793008 1102 42-43 7.216901888113636 1102 44-45 6.603595848150928 1102 46-47 5.79513423825064 1102 48-49 4.107893858123667 1102 50-51 5.399124492379773 1102 52-53 6.115316762081335 1102 54-55 5.649826039331426 1102 56-57 6.560026773371991 1102 58-59 6.238011128945946 1102 60-61 6.3197995513773755 1102 62-63 4.594962295619574 1102 64-65 6.303969179033597 1102 66-67 4.119036922901817 1102 68-69 6.3447274279862285 1102 70-71 5.6050003471326555 1102 72-73 5.286316041858154 1102 74-75 4.369029770614148 1102 76-77 3.5185547740285337 1102 78-79 4.61019509008365 1102 80-81 4.666358796425026 1102 82-83 4.144956779740525 1102 84-85 3.2648695706184405 1102 86-87 3.35724535372794 1102 88-89 3.6077804872919934 1102 90-91 3.466574215481362 1102 92-93 3.4238877586372034 1102 94-95 3.2202800804270595 1102 96-97 3.361954096517163 1102 98-99 3.2327918571342416 1102 100 3.5224183926906107 1103 1 6.745279543931101 1103 2 10.95029132101697 1103 3 8.048184400521869 1103 4 12.006798265813025 1103 5 10.577472693209248 1103 6 9.347216125309355 1103 7 8.347888359142193 1103 8 8.167540366252645 1103 9 7.2570073169727465 1103 10-11 7.5832778587343626 1103 12-13 8.885019038418221 1103 14-15 8.766577433901482 1103 16-17 8.2091643430476 1103 18-19 7.402824255319768 1103 20-21 8.628191101000056 1103 22-23 7.16409293348082 1103 24-25 5.61024349662749 1103 26-27 6.286064282707304 1103 28-29 7.294204710855393 1103 30-31 6.428325812639038 1103 32-33 8.241278751788936 1103 34-35 7.399867920622125 1103 36-37 4.4963517482783 1103 38-39 6.7115300804796405 1103 40-41 7.12947957509509 1103 42-43 7.734596400446183 1103 44-45 6.035859573773159 1103 46-47 6.657816358755547 1103 48-49 5.997849909384943 1103 50-51 6.848196923789157 1103 52-53 6.531352266066399 1103 54-55 5.305027429061507 1103 56-57 6.21245910393834 1103 58-59 5.76548211988462 1103 60-61 5.339179616431789 1103 62-63 3.1514027608663717 1103 64-65 5.451318745452273 1103 66-67 4.579512230895016 1103 68-69 5.5668217042416686 1103 70-71 4.197605631570454 1103 72-73 4.859127036918846 1103 74-75 3.6687619862690113 1103 76-77 3.974356273962549 1103 78-79 3.400291216166643 1103 80-81 4.497321255025639 1103 82-83 3.8188889340656225 1103 84-85 3.70495497772632 1103 86-87 3.07636364210574 1103 88-89 3.307918296773 1103 90-91 2.9588923115385057 1103 92-93 2.604132377051286 1103 94-95 3.1409450452282925 1103 96-97 3.2754848091372786 1103 98-99 3.1405578232800373 1103 100 3.30640957607487 1104 1 6.459093962277091 1104 2 9.776394891789227 1104 3 7.492383010531441 1104 4 12.244348034491004 1104 5 9.773531438561061 1104 6 8.412602066560662 1104 7 4.235875116793601 1104 8 6.776314377188545 1104 9 6.778729259918194 1104 10-11 7.20729735249806 1104 12-13 7.634539584623376 1104 14-15 7.460222183960781 1104 16-17 7.350442217076914 1104 18-19 6.986388552158882 1104 20-21 7.679284950295184 1104 22-23 6.5712058745146145 1104 24-25 6.115471157210806 1104 26-27 8.364873418911646 1104 28-29 7.39401820869757 1104 30-31 6.4733909182568325 1104 32-33 7.76075830182501 1104 34-35 8.13967466674313 1104 36-37 6.826312098373869 1104 38-39 7.476880575658406 1104 40-41 7.456703095265603 1104 42-43 7.426454373605818 1104 44-45 6.956812300784247 1104 46-47 6.875512655174099 1104 48-49 5.701054223217401 1104 50-51 5.921420906807672 1104 52-53 6.2243169577727215 1104 54-55 4.9756097664031635 1104 56-57 6.220268885274184 1104 58-59 4.206855734469823 1104 60-61 4.556994888524667 1104 62-63 4.032232091411242 1104 64-65 3.0064027673863727 1104 66-67 3.543024351634557 1104 68-69 4.430469415712306 1104 70-71 2.8340191603062515 1104 72-73 2.6892588039616534 1104 74-75 3.4508577049434663 1104 76-77 4.104850753628483 1104 78-79 4.421587676436353 1104 80-81 3.2853105902360795 1104 82-83 3.186395770623715 1104 84-85 3.2760036863599744 1104 86-87 0.9295219122038816 1104 88-89 0.485454554591211 1104 90-91 2.4394175642922775 1104 92-93 2.7574483140245896 1104 94-95 2.775205959846371 1104 96-97 3.0210076818212563 1104 98-99 2.666166623252 1104 100 1.006311016893278 1105 1 5.7866616547677125 1105 2 -2.7887944276684706 1105 3 0.49449668561128846 1105 4 8.472211503118007 1105 5 8.859367500827187 1105 6 8.323068825229672 1105 7 5.521671278577287 1105 8 6.242122172235906 1105 9 5.12119703864856 1105 10-11 5.269258826164332 1105 12-13 5.365240406803338 1105 14-15 6.126544646329485 1105 16-17 6.279562433173435 1105 18-19 6.314311907733309 1105 20-21 7.263185404477866 1105 22-23 2.2038382477157974 1105 24-25 4.286266920080223 1105 26-27 7.1222929842571965 1105 28-29 6.222446242888825 1105 30-31 5.551028137745618 1105 32-33 7.365176877265494 1105 34-35 6.535542449258873 1105 36-37 6.5612173863469465 1105 38-39 4.292608202975217 1105 40-41 5.823813850256805 1105 42-43 5.910240152389246 1105 44-45 6.331616368677377 1105 46-47 5.630140291574023 1105 48-49 5.210733812200775 1105 50-51 6.175755664972808 1105 52-53 5.8606720568524295 1105 54-55 5.584894036330741 1105 56-57 5.406774109239866 1105 58-59 2.0483347582356046 1105 60-61 4.537432703496005 1105 62-63 -0.5857042078217312 1105 64-65 -0.11694320774075706 1105 66-67 1.1109472978803865 1105 68-69 3.0296471723615674 1105 70-71 3.1793598552755746 1105 72-73 2.8453192262963967 1105 74-75 2.931445875727313 1105 76-77 2.0089596973406394 1105 78-79 3.3974504383376036 1105 80-81 1.8096928459137729 1105 82-83 1.313128659171415 1105 84-85 1.6388707180475315 1105 86-87 0.07821772517983305 1105 88-89 1.8775456921470806 1105 90-91 1.5710889661603176 1105 92-93 1.8331897353529456 1105 94-95 1.2286406297794557 1105 96-97 2.2512491866001234 1105 98-99 2.1131049947562843 1105 100 2.263299048469179 1106 1 6.180689460087212 1106 2 10.392514239549438 1106 3 7.024107980923045 1106 4 11.90261788295792 1106 5 10.126614707355994 1106 6 9.461708971013106 1106 7 6.697989151916598 1106 8 7.491182329773842 1106 9 6.924494511717654 1106 10-11 5.940474222819319 1106 12-13 8.02235591971506 1106 14-15 7.99981170046393 1106 16-17 7.807677795693959 1106 18-19 7.298971766484502 1106 20-21 8.349367547797435 1106 22-23 6.389008638617749 1106 24-25 5.154525632987877 1106 26-27 7.832077588934919 1106 28-29 6.439306298874268 1106 30-31 5.506090224774248 1106 32-33 7.549516571236321 1106 34-35 6.900166076067816 1106 36-37 6.129693409578614 1106 38-39 7.242728293814096 1106 40-41 6.9139131528701725 1106 42-43 6.453724104391046 1106 44-45 4.644949147967857 1106 46-47 6.150487304651428 1106 48-49 5.964685434008029 1106 50-51 6.715569513066622 1106 52-53 5.801092539156073 1106 54-55 5.451859644655954 1106 56-57 5.1135252353200045 1106 58-59 5.26889869720145 1106 60-61 5.108864908145858 1106 62-63 5.0504307678158185 1106 64-65 5.276920708422082 1106 66-67 3.2304865400556935 1106 68-69 3.695049450359331 1106 70-71 3.221519136262817 1106 72-73 3.076124041999524 1106 74-75 3.0295983518290015 1106 76-77 3.6750792466891973 1106 78-79 4.258951645243187 1106 80-81 3.8547816626624254 1106 82-83 2.283952092149651 1106 84-85 2.931734994076237 1106 86-87 3.523798665595365 1106 88-89 3.434949432640291 1106 90-91 3.2572844587916094 1106 92-93 3.068314556116267 1106 94-95 1.6624156135212473 1106 96-97 1.7656715519888095 1106 98-99 2.208603302026649 1106 100 3.119903058743894 1107 1 6.057914343093994 1107 2 10.406563899795906 1107 3 6.358704375987799 1107 4 11.524534675064977 1107 5 9.562470116621075 1107 6 8.15474450580977 1107 7 4.049370689859796 1107 8 6.5847045311215915 1107 9 5.846068874887749 1107 10-11 4.6413744287554906 1107 12-13 6.420824559150333 1107 14-15 6.417245725571611 1107 16-17 7.030603772942587 1107 18-19 6.36759007175775 1107 20-21 5.703928693561217 1107 22-23 5.870024565777282 1107 24-25 4.748457488286029 1107 26-27 7.328209971305544 1107 28-29 6.539497379640206 1107 30-31 5.1268938943290365 1107 32-33 5.193450409098254 1107 34-35 6.7792400616725494 1107 36-37 6.597703238541094 1107 38-39 6.432928916908359 1107 40-41 7.771178928735203 1107 42-43 6.248197816001888 1107 44-45 4.345418809033372 1107 46-47 3.6425156092217605 1107 48-49 4.158176836495766 1107 50-51 5.064681503394446 1107 52-53 4.122937324676666 1107 54-55 5.430777022359333 1107 56-57 5.379943734009512 1107 58-59 3.1425875825585514 1107 60-61 3.02486242023161 1107 62-63 0.5003219805238999 1107 64-65 2.3267836187717172 1107 66-67 4.302490255955966 1107 68-69 5.146801638278667 1107 70-71 4.4462586877206896 1107 72-73 2.5537323746354055 1107 74-75 2.794089949775735 1107 76-77 3.402070764536454 1107 78-79 3.977303072059698 1107 80-81 2.388052978126183 1107 82-83 1.7938765478496954 1107 84-85 2.545877037891646 1107 86-87 2.533788786605264 1107 88-89 2.6999919361079883 1107 90-91 1.5584524702241893 1107 92-93 2.3981083245946486 1107 94-95 2.4236583928174014 1107 96-97 2.5558593152538442 1107 98-99 2.0394912944591432 1107 100 0.3829671701075732 1108 1 4.422783986869998 1108 2 -3.328813874504114 1108 3 2.7698702780298845E-6 1108 4 6.807931665021535 1108 5 7.3563904975060055 1108 6 7.12574190308742 1108 7 4.699904744780351 1108 8 5.438233870423353 1108 9 5.109411702681715 1108 10-11 3.603574214133051 1108 12-13 2.8740289763371933 1108 14-15 4.4250101477270185 1108 16-17 4.992293514519815 1108 18-19 4.290493715684349 1108 20-21 5.103802014230041 1108 22-23 4.4718794092166085 1108 24-25 3.202156003987646 1108 26-27 4.935178504946908 1108 28-29 6.396023579687213 1108 30-31 4.813347662659659 1108 32-33 4.86581303872557 1108 34-35 3.315936750387202 1108 36-37 1.5229481779505107 1108 38-39 2.728414463016385 1108 40-41 5.737034773023545 1108 42-43 3.4975105694551196 1108 44-45 4.0482083921228345 1108 46-47 4.895666161512274 1108 48-49 5.060782321430754 1108 50-51 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-18.385201810301673 2213 54-55 -18.40056528313815 2213 56-57 -18.264010429068236 2213 58-59 -18.70994983017414 2213 60-61 -20.05978645483836 2213 62-63 -18.290668641380172 2213 64-65 -18.37750844208571 2213 66-67 -9.245461847606965 2213 68-69 -23.43569804514542 2213 70-71 -18.110387725960187 2213 72-73 -9.903413965556254 2213 74-75 -4.732836098998938 2213 76-77 -3.410329836530842 2213 78-79 -4.9758049222043255 2213 80-81 -3.6010900395172243 2213 82-83 -3.637836546620374 2213 84-85 -3.4351802947786823 2213 86-87 -3.5718414138467907 2213 88-89 -3.140499622261256 2213 90-91 -3.260804919893083 2213 92-93 -3.986698193471799 2213 94-95 -3.989206434808146 2213 96-97 -3.5382629646232466 2213 98-99 -4.148464104763523 2213 100 -3.632218009088927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1104.0 3 1215.0 4 9715.0 5 32927.0 6 35459.0 7 40566.0 8 36213.0 9 35470.0 10 40007.0 11 47850.0 12 57503.0 13 77088.0 14 56135.0 15 61472.0 16 70757.0 17 75055.0 18 74573.0 19 73646.0 20 70699.0 21 67702.0 22 69369.0 23 77689.0 24 84954.0 25 94970.0 26 104621.0 27 112563.0 28 122454.0 29 128053.0 30 132698.0 31 142750.0 32 161978.0 33 197264.0 34 245342.0 35 297142.0 36 339419.0 37 316055.0 38 108217.0 39 344.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.262013429592564 22.05528517608242 14.274153771658476 23.408547622666536 2 14.632520725477475 21.791730769158274 41.22657580919932 22.349172696164935 3 17.216210545247446 27.594361385932032 30.43626730989749 24.753160758923034 4 11.970991120436222 18.143437719809782 40.39341066629794 29.492160493456055 5 12.704521554915438 38.04316148537954 36.56708613102006 12.68523082868496 6 29.079686595391564 39.80612142498746 18.82870045158053 12.285491528040449 7 25.60353502630314 32.66089982979704 24.894551430549765 16.841013713350065 8 23.072369530316468 37.0805567977167 22.473994032739295 17.37307963922753 9 23.90295235991116 17.616629263130967 23.06276817172293 35.41765020523495 10-11 22.590953494743108 28.19326097722753 29.655599452566083 19.56018607546328 12-13 23.11961650557814 25.90483537789865 30.071009695007234 20.904538421515976 14-15 20.766339164787322 27.30753073120946 27.19758520069241 24.728544903310805 16-17 20.22636166738481 31.207278285196548 28.08137589929953 20.48498414811911 18-19 20.153875574476977 30.092176016823878 29.734497421733458 20.019450986965683 20-21 21.789150930759195 29.41578947843711 28.556145515164776 20.238914075638917 22-23 20.577591508160634 29.474933662978593 29.278480619109526 20.668994209751247 24-25 20.95945522234443 29.088778248499654 28.757107995204446 21.194658533951472 26-27 20.931770344264468 29.20030998790764 28.86226866706123 21.005651000766658 28-29 20.60973718590382 29.08393330011898 29.328696479960865 20.97763303401634 30-31 21.34197842768848 29.501856422369055 28.503927503853426 20.65223764608904 32-33 20.310458283050863 29.36383886710045 29.391096559502177 20.93460629034651 34-35 20.561484387640384 28.55389437117937 29.16452512756577 21.720096113614474 36-37 21.114472112162787 29.433909269038555 28.659720289270563 20.791898329528095 38-39 20.780681133593067 29.06122654988425 28.9702739494087 21.18781836711398 40-41 21.152671833242067 29.133319682572832 28.481023497849243 21.23298498633585 42-43 20.63441439220826 29.293575386625164 29.173673693350572 20.898336527816 44-45 21.20773396510048 28.670594019345092 28.90648889389761 21.21518312165682 46-47 21.005593453828393 29.28483728887141 29.023478201095532 20.68609105620466 48-49 20.770036597533245 28.909459027355926 29.23105299627615 21.089451378834674 50-51 21.224897712353716 29.028580689834584 28.726969539036713 21.01955205877499 52-53 20.51195813251652 29.058953652260968 29.11214212811995 21.316946087102565 54-55 21.024340671322765 28.94098666515899 28.660908273785967 21.373764389732276 56-57 21.165673845857526 29.033430950260637 28.655707793365824 21.145187410516016 58-59 20.768509244053558 28.509971590533663 29.173337531827965 21.54818163358481 60-61 21.349920141919014 29.025731411966753 28.480190828956232 21.144157617157997 62-63 20.676046211002532 29.194425076343087 29.09942190605991 21.03010680659447 64-65 20.731266775125793 28.518157178781365 29.069133539726018 21.681442506366825 66-67 20.95698643733102 28.87136240832038 28.79328519176896 21.378365962579643 68-69 21.283130614787606 28.774554154036853 28.786391768689235 21.15592346248631 70-71 21.47024943787769 28.851950901658046 28.367007906764535 21.310791753699725 72-73 20.958561833741612 29.064159748692525 29.04362944755794 20.933648970007926 74-75 21.377402733146333 28.397619245186608 28.60122384216119 21.623754179505873 76-77 21.32074660805274 29.048068828522 28.632836512762548 20.99834805066271 78-79 21.21339465008481 28.668817794674574 28.918423807491035 21.199363747749583 80-81 21.480500210923783 28.668215987619877 28.512978306311894 21.338305495144446 82-83 20.75729427654391 29.01474095884524 29.09677292966882 21.13119183494203 84-85 21.119382732546367 28.40823023271512 28.630186428326414 21.8422006064121 86-87 21.333904051204858 28.829466429957307 28.65463766909113 21.1819918497467 88-89 21.03696847611577 28.351282155151637 29.028084835683615 21.583664533048978 90-91 21.48577384608517 28.457735095946607 28.529537828927953 21.52695322904027 92-93 20.882297813773523 28.780995282365556 28.993668727706634 21.343038176154288 94-95 20.972247852277118 28.19821062389929 28.80722161512686 22.022319908696733 96-97 21.2478030388933 28.544698618367686 28.737123518277848 21.47037482446117 98-99 21.125924862871663 28.288684827770936 28.83789127663863 21.74749903271877 100 21.418214307636696 28.09905811582243 28.503529508051372 21.97919806848951 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 448.0 1 517.0 2 509.5 3 439.5 4 554.5 5 887.5 6 1490.0 7 2391.5 8 3710.5 9 5414.5 10 7127.5 11 8880.5 12 10547.0 13 11959.0 14 13164.5 15 14217.5 16 15362.0 17 16522.0 18 17548.0 19 18829.0 20 20396.0 21 22093.5 22 24149.0 23 27019.5 24 30380.5 25 34670.5 26 40735.0 27 48413.0 28 57850.5 29 68833.5 30 80406.0 31 92442.5 32 104216.0 33 114574.0 34 124051.5 35 132037.5 36 139106.0 37 144883.0 38 148191.5 39 149891.0 40 149917.0 41 146980.5 42 141625.5 43 135171.0 44 127539.5 45 119472.0 46 111460.0 47 104098.5 48 97715.5 49 93179.5 50 90344.5 51 83864.0 52 73513.5 53 64978.5 54 58579.0 55 52749.5 56 47468.0 57 42546.0 58 37687.0 59 32969.0 60 28098.5 61 23142.0 62 18805.5 63 14940.5 64 11612.0 65 8985.5 66 7038.5 67 5530.5 68 4128.5 69 3093.0 70 2399.5 71 1808.0 72 1343.5 73 988.5 74 726.5 75 535.5 76 392.5 77 284.0 78 186.5 79 130.5 80 100.5 81 65.0 82 48.0 83 37.0 84 30.5 85 27.0 86 20.0 87 12.5 88 9.0 89 6.0 90 1.5 91 2.5 92 3.0 93 3.5 94 3.5 95 1.5 96 1.0 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 10.050477730841996 2 28.32678291873793 3 8.057631399623565 4 28.090659971607966 5 15.261015963629662 6 12.494143534867785 7 10.132535791310438 8 11.542005242853492 9 7.05418317780039 10-11 8.670648612632457 12-13 12.651585852401407 14-15 9.538310603673889 16-17 10.266876481678922 18-19 8.444144588626218 20-21 10.239424723550528 22-23 7.444870871360953 24-25 6.673938500496347 26-27 12.209520680414522 28-29 8.26577570940909 30-31 5.5335016825009635 32-33 11.03445844111841 34-35 9.237638197716425 36-37 9.189354445968942 38-39 7.92261522308066 40-41 10.86388467235408 42-43 8.250104430162565 44-45 8.413653142712937 46-47 7.825899112627323 48-49 5.148704228381336 50-51 8.80825865608513 52-53 6.046438863907909 54-55 6.331467010065825 56-57 6.2925454966957926 58-59 4.926091004739751 60-61 5.64451108040501 62-63 3.79975833806624 64-65 5.533312546372126 66-67 3.276067416762541 68-69 6.987809906301962 70-71 3.559879687806502 72-73 2.2813194568658846 74-75 1.1742246364398312 76-77 1.6098861994932232 78-79 2.4627955724853408 80-81 2.739677355379761 82-83 1.3845169922600093 84-85 1.9317013227100073 86-87 0.5483191472230223 88-89 0.9510440044117352 90-91 0.43073051398013207 92-93 0.6050599858742332 94-95 0.8750247903426012 96-97 0.8736467985467862 98-99 0.5920366124314314 100 0.9595416204859285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3701038.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.74009462577338 #Duplication Level Percentage of deduplicated Percentage of total 1 96.34827664031049 91.28044845933206 2 2.9965009377657963 5.677775647803005 3 0.4177598819194041 1.1873583223168878 4 0.1155233534497583 0.43778773749267064 5 0.040528810105547035 0.19198516522347633 6 0.023009236748166687 0.13079383600728803 7 0.013651092828445576 0.09053140784185054 8 0.01023209609536313 0.07755118018357673 9 0.006750554154186034 0.057559332540959324 >10 0.026233903493933466 0.3963268738296046 >50 4.65465150517328E-4 0.03198444569380471 >100 8.100911231866473E-4 0.1773360316615019 >500 2.0061756309686506E-4 0.13369733370853892 >1k 5.731929213504441E-5 0.1288642263647507 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.7019446976767058E-5 0.0 0.0 0.0 3 0.0 2.7019446976767058E-5 0.0 0.0 0.0 4 0.0 2.7019446976767058E-5 0.0 2.7019446976767058E-5 0.0 5 0.0 2.7019446976767058E-5 0.0 5.4038893953534116E-5 0.0 6 0.0 2.7019446976767058E-5 0.0 5.4038893953534116E-5 0.0 7 0.0 2.7019446976767058E-5 0.0 5.4038893953534116E-5 0.0 8 0.0 2.7019446976767058E-5 0.0 5.4038893953534116E-5 0.0 9 0.0 2.7019446976767058E-5 0.0 1.0807778790706823E-4 0.0 10-11 1.3509723488383529E-5 2.7019446976767058E-5 0.0 1.350972348838353E-4 0.0 12-13 2.7019446976767058E-5 4.0529170465150585E-5 0.0 1.4860695837221882E-4 0.0 14-15 2.7019446976767058E-5 5.4038893953534116E-5 0.0 1.6211668186060234E-4 0.0 16-17 2.7019446976767058E-5 5.4038893953534116E-5 0.0 1.756264053489859E-4 0.0 18-19 4.0529170465150585E-5 5.4038893953534116E-5 0.0 2.0264585232575294E-4 0.0 20-21 5.4038893953534116E-5 5.4038893953534116E-5 0.0 2.5668474627928704E-4 0.0 22-23 8.105834093030117E-5 5.4038893953534116E-5 0.0 3.1072364023282113E-4 0.0 24-25 8.105834093030117E-5 5.4038893953534116E-5 0.0 4.052917046515059E-4 0.0 26-27 8.105834093030117E-5 5.4038893953534116E-5 0.0 5.133694925585741E-4 0.0 28-29 8.105834093030117E-5 8.105834093030117E-5 0.0 7.565445153494776E-4 0.0 30-31 1.0807778790706823E-4 1.0807778790706823E-4 0.0 0.0021885752051181316 0.0 32-33 1.0807778790706823E-4 1.0807778790706823E-4 0.0 0.00643062838047056 0.0 34-35 1.0807778790706823E-4 1.0807778790706823E-4 0.0 0.013280058189081009 0.0 36-37 1.0807778790706823E-4 1.0807778790706823E-4 0.0 0.023101627165135835 0.0 38-39 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.039434882862591523 0.0 40-41 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.060820775144702646 0.0 42-43 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.0807881464605335 0.0 44-45 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.1046598278645072 0.0 46-47 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.1359213280166267 0.0 48-49 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.16895260194572442 0.0 50-51 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.2034834551820327 0.0 52-53 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.24040552947578492 0.0 54-55 1.0807778790706823E-4 1.350972348838353E-4 0.0 0.2826504348239602 0.0 56-57 1.0807778790706823E-4 1.4860695837221882E-4 0.0 0.3319609255565601 0.0 58-59 1.0807778790706823E-4 1.6211668186060234E-4 0.0 0.3872427140710255 0.0 60-61 1.0807778790706823E-4 1.6211668186060234E-4 0.0 0.44536154451805143 0.0 62-63 1.0807778790706823E-4 1.756264053489859E-4 0.0 0.5052096195715905 0.0 64-65 1.0807778790706823E-4 1.8913612883736942E-4 0.0 0.5760140803742084 0.0 66-67 1.0807778790706823E-4 1.8913612883736942E-4 0.0 0.660814614710792 0.0 68-69 1.0807778790706823E-4 1.8913612883736942E-4 0.0 0.7541127651215687 0.0 70-71 1.0807778790706823E-4 1.8913612883736942E-4 0.0 0.8701477801632947 0.0 72-73 1.0807778790706823E-4 1.8913612883736942E-4 0.0 0.9900735955696753 0.0 74-75 1.0807778790706823E-4 1.8913612883736942E-4 0.0 1.1152682031365253 1.3509723488383529E-5 76-77 1.0807778790706823E-4 1.8913612883736942E-4 0.0 1.258754976306647 2.7019446976767058E-5 78-79 1.0807778790706823E-4 1.8913612883736942E-4 0.0 1.4213309887658543 2.7019446976767058E-5 80-81 1.0807778790706823E-4 1.8913612883736942E-4 0.0 1.6037257655825203 2.7019446976767058E-5 82-83 1.0807778790706823E-4 1.8913612883736942E-4 0.0 1.8019404286040834 2.7019446976767058E-5 84-85 1.0807778790706823E-4 1.8913612883736942E-4 0.0 2.0041944989486735 2.7019446976767058E-5 86-87 1.0807778790706823E-4 2.0264585232575294E-4 0.0 2.2268347420372336 2.7019446976767058E-5 88 1.0807778790706823E-4 2.1615557581413646E-4 0.0 2.4175379987992556 2.7019446976767058E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 780 0.0 19.625177 1 TAGACCG 240 1.6583726E-6 19.013226 5 GTATACG 255 1.8596233E-4 17.508736 1 TAGTGCG 180 0.0016498662 16.968054 9 GTACATG 12710 0.0 16.861284 1 TACATGG 13070 0.0 16.441843 2 CATGGGT 2235 0.0 16.212652 4 TATATCG 315 1.2365908E-6 16.095852 5 TAGTACG 215 0.0023916212 16.051065 4 GTATAAG 1610 0.0 15.846416 1 ACATGGG 13295 0.0 15.79049 3 CCGCGCT 195 0.0028160848 15.662821 9 GTATAGG 995 0.0 15.384561 1 GTATTAG 1540 0.0 15.324204 1 GTCTAGG 980 0.0 14.969203 1 GTCTCGC 470 4.4418994E-7 14.927661 1 GTATCAA 9280 0.0 14.639579 1 GTGTAAG 1420 0.0 14.373369 1 CTATCCG 215 0.0053905137 14.205813 9 GAGTACG 315 9.278221E-4 14.173739 1 >>END_MODULE