FastQCFastQC Report
Wed 25 May 2016
SRR1295160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701038
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74610.20159209389365904No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54200.14644540261407746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT2453.45608E-1022.9908076
GTATCAA133150.017.7391381
TACCGTG3351.6922058E-816.8128097
TATACCG2253.870612E-516.7144765
GAGTACT50800.016.676312-13
GTACCGT1700.001936305316.5669046
CAACGCA146050.015.964875
GTACATG169450.015.9388321
ATCAACG147500.015.9351963
TCAACGC147600.015.8290674
AACGCAG148400.015.7519566
TCTGTCG6650.015.5276198
TATCAAC151250.015.3376192
TACATGG175900.015.2857382
GTATACG2658.390019E-515.268661
ACATGGG170150.015.1953323
TACACCG2509.526028E-515.0430285
TAATACG3859.177165E-814.65234
GTCTAGG12200.014.5099211
ACGCAGA162100.014.3615897
Produced by FastQC (version 0.11.5)