FastQCFastQC Report
Wed 25 May 2016
SRR1295159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189863
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC1250.002231104822.5590024
GTGTAGG10550.017.776631
GTACATG105500.017.1248191
TACATGG106550.016.539952
GTATTAG12350.016.1980841
ACATGGG108900.015.1221953
GTATTAT19150.015.0165341
TATACTG17100.014.93864255
GTACTAA9850.013.9626251
GTATTAA18050.013.8536231
GTATATA23300.013.6834271
GTACTGT11950.013.4110346
ATAGCGC2950.001431686213.3824583
GTGTATA18750.013.3364221
CTTACAC16550.013.2900463
CCTACAC10650.013.2388513
CCGTGCA3455.7490965E-513.0805029
GTCTAGG8451.6189006E-1012.5768781
CATGGGG36800.012.56683354
TCTGTCG6801.70985E-1012.5317218
Produced by FastQC (version 0.11.5)