##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295159_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3189863
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.979144245379818	27.0	27.0	33.0	6.0	33.0
2	21.98963591853318	27.0	2.0	33.0	2.0	33.0
3	24.396462481304056	27.0	15.0	33.0	15.0	33.0
4	24.465973930541846	33.0	6.0	37.0	2.0	37.0
5	26.374845878960947	33.0	15.0	37.0	2.0	37.0
6	27.0272293825785	33.0	15.0	37.0	2.0	37.0
7	26.95109351091254	33.0	15.0	37.0	6.0	37.0
8	27.70562591559575	33.0	15.0	37.0	6.0	37.0
9	28.756044695336445	33.0	22.0	37.0	15.0	37.0
10-11	28.23014687464634	33.0	18.5	37.0	10.5	37.0
12-13	27.0551250947141	33.0	15.0	37.0	4.0	37.0
14-15	28.811293933313124	33.0	22.0	37.0	6.0	40.0
16-17	29.179004082620477	33.0	22.0	37.0	6.0	40.0
18-19	29.319164804256484	33.0	22.0	38.5	6.0	40.0
20-21	28.846131636374352	33.0	22.0	37.0	6.0	40.0
22-23	29.578439262125052	33.0	24.5	37.0	6.0	40.0
24-25	29.694361481982142	33.0	24.5	37.0	6.0	40.0
26-27	28.2464761025787	33.0	18.5	37.0	4.0	40.0
28-29	28.960543133043643	33.0	22.0	37.0	6.0	40.0
30-31	29.646748465372966	33.0	24.5	37.0	10.5	40.0
32-33	28.449528553420635	33.0	22.0	37.0	6.0	40.0
34-35	28.09391124321013	33.0	15.0	37.0	6.0	40.0
36-37	28.417507742495523	33.0	22.0	37.0	6.0	40.0
38-39	28.518968682981054	33.0	22.0	37.0	6.0	40.0
40-41	27.57538254777713	33.0	15.0	37.0	6.0	40.0
42-43	28.17380809144468	33.0	18.5	37.0	6.0	40.0
44-45	28.05948923198269	33.0	22.0	37.0	6.0	40.0
46-47	27.741125089071225	33.0	18.5	37.0	6.0	40.0
48-49	27.677037070244083	33.0	18.5	37.0	6.0	40.0
50-51	27.63101597153232	33.0	22.0	37.0	6.0	40.0
52-53	27.845267492679152	33.0	22.0	37.0	6.0	40.0
54-55	27.830823925667026	33.0	22.0	37.0	6.0	40.0
56-57	27.819951671905656	33.0	22.0	37.0	6.0	40.0
58-59	27.69221797299759	33.0	22.0	37.0	6.0	40.0
60-61	27.6616182262373	33.0	22.0	37.0	6.0	40.0
62-63	27.652776310455966	33.0	22.0	37.0	6.0	40.0
64-65	27.197013163261243	33.0	22.0	37.0	6.0	40.0
66-67	27.414378611244434	33.0	22.0	37.0	6.0	40.0
68-69	27.006929451202137	33.0	22.0	37.0	6.0	37.0
70-71	27.15652396356834	33.0	22.0	37.0	6.0	37.0
72-73	27.2195730663041	33.0	22.0	37.0	6.0	37.0
74-75	27.32490125751482	27.0	22.0	37.0	6.0	37.0
76-77	27.068214528335545	27.0	22.0	37.0	6.0	37.0
78-79	26.627626327525665	27.0	22.0	35.0	6.0	37.0
80-81	26.454538643195647	27.0	22.0	33.0	6.0	37.0
82-83	26.558157356601207	27.0	22.0	33.0	6.0	37.0
84-85	26.51107618101467	27.0	22.0	33.0	6.0	37.0
86-87	26.32857367228624	27.0	22.0	33.0	10.5	37.0
88-89	26.263031986013193	27.0	22.0	33.0	6.0	37.0
90-91	26.408614100354782	27.0	22.0	33.0	10.5	37.0
92-93	26.327378166397743	27.0	22.0	33.0	6.0	37.0
94-95	26.180017449025243	27.0	22.0	33.0	10.5	37.0
96-97	26.25508948189938	27.0	22.0	33.0	6.0	37.0
98-99	26.317987324220507	27.0	22.0	33.0	10.5	37.0
100	25.994778772630674	27.0	22.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.265044441292218
1101	2	11.16873149096553
1101	3	8.382161069691637
1101	4	12.722559043050477
1101	5	10.811664811176968
1101	6	9.993440040221206
1101	7	9.59199490011439
1101	8	9.037297395640081
1101	9	7.222149089711369
1101	10-11	7.803031128916452
1101	12-13	9.113786012693943
1101	14-15	7.9519850980700575
1101	16-17	8.04110531827541
1101	18-19	8.082692443133652
1101	20-21	7.22666204535647
1101	22-23	7.420942774651085
1101	24-25	7.3484267832686605
1101	26-27	8.545512783843684
1101	28-29	8.206511623692954
1101	30-31	5.56152415480361
1101	32-33	7.065232602713827
1101	34-35	8.763478497674413
1101	36-37	8.065737147955222
1101	38-39	8.144809018671214
1101	40-41	8.91382186236655
1101	42-43	8.486587417199122
1101	44-45	8.434418729006428
1101	46-47	7.2845205177218695
1101	48-49	5.3637805557706955
1101	50-51	6.732650345174054
1101	52-53	5.964058744782424
1101	54-55	4.274412761786177
1101	56-57	6.1859445615755995
1101	58-59	6.17816934112475
1101	60-61	6.629016264190199
1101	62-63	6.249616227372162
1101	64-65	6.784412303657181
1101	66-67	5.813669070786098
1101	68-69	6.810454532367974
1101	70-71	6.286458597677349
1101	72-73	5.521386258076422
1101	74-75	4.918743208581194
1101	76-77	5.1199101276225605
1101	78-79	5.252238694900491
1101	80-81	4.962094686698983
1101	82-83	4.526301509245876
1101	84-85	4.351577911784833
1101	86-87	4.152340885557269
1101	88-89	4.067317546935826
1101	90-91	3.8752871065144703
1101	92-93	3.90920591050919
1101	94-95	3.7477416423273304
1101	96-97	3.9088545621610606
1101	98-99	3.618808294782056
1101	100	3.647058943103872
1102	1	6.576307875274626
1102	2	10.268107688518104
1102	3	7.880523189173449
1102	4	12.339219005667072
1102	5	10.337495762255791
1102	6	9.283759753907585
1102	7	6.580409302499
1102	8	7.637447129078076
1102	9	6.216814200690909
1102	10-11	7.168656640679458
1102	12-13	8.750086630262928
1102	14-15	6.015173401660139
1102	16-17	7.105323164138152
1102	18-19	7.194795201017676
1102	20-21	6.6039806548293285
1102	22-23	6.230649189384469
1102	24-25	6.587374950391503
1102	26-27	8.177019292491693
1102	28-29	7.434273634404086
1102	30-31	4.120157599354677
1102	32-33	4.332746354342234
1102	34-35	4.9232282751593175
1102	36-37	4.947146527514938
1102	38-39	4.440984745434406
1102	40-41	5.960669022187773
1102	42-43	6.627255422938251
1102	44-45	6.140964625758556
1102	46-47	5.334651398733957
1102	48-49	3.6145671884450756
1102	50-51	4.890512295461853
1102	52-53	5.620971688275969
1102	54-55	5.127908833401072
1102	56-57	6.038819665122762
1102	58-59	5.71785338271998
1102	60-61	5.7948859235563965
1102	62-63	4.118625988498021
1102	64-65	5.920790263955016
1102	66-67	3.6390126062936012
1102	68-69	5.857198481673194
1102	70-71	5.102525838285995
1102	72-73	4.819008843369527
1102	74-75	3.9897670299679255
1102	76-77	3.124477963993929
1102	78-79	4.143217819965486
1102	80-81	4.243509604701792
1102	82-83	3.6547398290169006
1102	84-85	2.84606633556999
1102	86-87	3.0539085798838634
1102	88-89	3.2408975552847608
1102	90-91	3.161321759358337
1102	92-93	2.984388994062357
1102	94-95	2.9040308485316046
1102	96-97	3.164476049322083
1102	98-99	2.892718307461834
1102	100	2.9981053613384248
1103	1	6.667080472260238
1103	2	10.98428755582544
1103	3	7.942938673264177
1103	4	11.962836220369997
1103	5	10.376641137205837
1103	6	9.009909988081166
1103	7	7.917372407497854
1103	8	7.8825709354847575
1103	9	6.988036173463573
1103	10-11	7.440276006501101
1103	12-13	8.589442841684011
1103	14-15	8.401191581630233
1103	16-17	7.854674618682221
1103	18-19	7.180081057403321
1103	20-21	8.224683745194469
1103	22-23	6.745667533266857
1103	24-25	5.2186793143087336
1103	26-27	5.959623972507963
1103	28-29	6.917687873673422
1103	30-31	6.012330953669487
1103	32-33	7.858353825252628
1103	34-35	7.072619975478872
1103	36-37	4.02579897634628
1103	38-39	6.310097134459248
1103	40-41	6.744566989105202
1103	42-43	7.416394483449775
1103	44-45	5.629061700866782
1103	46-47	6.322011076781784
1103	48-49	5.553574009704985
1103	50-51	6.354361729341743
1103	52-53	5.975610331815307
1103	54-55	4.8609518911188125
1103	56-57	5.858885047625581
1103	58-59	5.408698823769914
1103	60-61	4.886740602629885
1103	62-63	2.7078891386889588
1103	64-65	5.032430183712535
1103	66-67	4.14675620176471
1103	68-69	5.1636475668206785
1103	70-71	3.624734990205159
1103	72-73	4.299791507499545
1103	74-75	3.223116425654233
1103	76-77	3.4951613217387063
1103	78-79	2.8960228667629266
1103	80-81	4.116412169670568
1103	82-83	3.3297603224394017
1103	84-85	3.2563156142550156
1103	86-87	2.6962544959637462
1103	88-89	2.9574434955875333
1103	90-91	2.530615008811374
1103	92-93	2.2535930871515646
1103	94-95	2.7278740412029236
1103	96-97	2.895511280030181
1103	98-99	2.7072067033217024
1103	100	2.808981613340297
1104	1	6.2894726984877
1104	2	9.667666619719451
1104	3	7.279252992654797
1104	4	11.996892936834708
1104	5	9.423615415879347
1104	6	8.12903620495712
1104	7	3.906230207473861
1104	8	6.469561480568025
1104	9	6.4113427995153
1104	10-11	7.0596934562331555
1104	12-13	7.496604738966759
1104	14-15	7.139906341485773
1104	16-17	6.978190335215455
1104	18-19	6.615692370714207
1104	20-21	7.367380468078373
1104	22-23	6.298472634584886
1104	24-25	5.748913891298404
1104	26-27	8.05668191291695
1104	28-29	7.043974574165901
1104	30-31	6.2285428337856565
1104	32-33	7.4612016316214
1104	34-35	7.843388361639285
1104	36-37	6.512040833232522
1104	38-39	7.181122332244801
1104	40-41	7.127154435150278
1104	42-43	7.124837056628802
1104	44-45	6.429020819049104
1104	46-47	6.502415232062052
1104	48-49	5.386555511904579
1104	50-51	5.569440100178191
1104	52-53	5.776082619641624
1104	54-55	4.638375121997019
1104	56-57	5.851003836456261
1104	58-59	3.716359714399008
1104	60-61	4.204257058849652
1104	62-63	3.8024464753171436
1104	64-65	2.7773079777731624
1104	66-67	3.2600136119740064
1104	68-69	4.12404672450462
1104	70-71	2.520695850764117
1104	72-73	2.418700521828047
1104	74-75	3.2011550989817756
1104	76-77	3.781923183190962
1104	78-79	4.286416987215041
1104	80-81	3.0437755333259418
1104	82-83	2.944007453137381
1104	84-85	2.9829398947165267
1104	86-87	0.8965452575977828
1104	88-89	0.49166974189945023
1104	90-91	2.3103574449924906
1104	92-93	2.468573976674385
1104	94-95	2.48673975418658
1104	96-97	2.874135479811425
1104	98-99	2.5615720869783836
1104	100	0.9637965604691807
1105	1	5.598806626414486
1105	2	-2.8112407560738397
1105	3	0.4586647150758516
1105	4	8.308689907073063
1105	5	8.392099405950908
1105	6	7.661403429123819
1105	7	5.050101149369151
1105	8	5.785652436535091
1105	9	4.66825494842011
1105	10-11	4.839582866759848
1105	12-13	4.99394493554728
1105	14-15	5.655383669293723
1105	16-17	5.83294195598522
1105	18-19	5.994408521360363
1105	20-21	6.869681683254633
1105	22-23	1.9411873584171566
1105	24-25	3.776603425851654
1105	26-27	6.593997992345148
1105	28-29	5.725346828824655
1105	30-31	5.116632488122544
1105	32-33	6.9210684144015175
1105	34-35	5.984829398090362
1105	36-37	6.108178051948297
1105	38-39	3.9964727274413967
1105	40-41	5.1073997996268865
1105	42-43	5.4364089065619225
1105	44-45	5.818147951007649
1105	46-47	5.150334175427286
1105	48-49	4.7006430023514305
1105	50-51	5.67794219192103
1105	52-53	5.362227757893368
1105	54-55	5.159073807585475
1105	56-57	4.979873990255154
1105	58-59	1.728475556854768
1105	60-61	4.194476313578711
1105	62-63	-0.8047008182335951
1105	64-65	-0.4357107961031339
1105	66-67	0.6375742458664995
1105	68-69	2.7328670353705142
1105	70-71	2.837828316881673
1105	72-73	2.3738027183815653
1105	74-75	2.3756042467769376
1105	76-77	1.731297654688607
1105	78-79	3.0583233932728113
1105	80-81	1.5367535637582073
1105	82-83	0.8607275362004607
1105	84-85	1.2650213353181705
1105	86-87	-0.19793310148558518
1105	88-89	1.3382877081829498
1105	90-91	1.1138990178225043
1105	92-93	1.431501120099341
1105	94-95	0.7821608001649487
1105	96-97	1.8504318151018246
1105	98-99	1.77907049632109
1105	100	1.860520165858535
1106	1	6.0527159403334565
1106	2	10.315022163923413
1106	3	6.766506320805945
1106	4	11.712001539774977
1106	5	9.926164483096464
1106	6	9.336015546516311
1106	7	6.401973431545347
1106	8	7.354744483368172
1106	9	6.8117120949712735
1106	10-11	5.523466403622827
1106	12-13	7.806302326470565
1106	14-15	7.735378437929608
1106	16-17	7.672020854002209
1106	18-19	7.167787289190635
1106	20-21	8.035939875395897
1106	22-23	6.136759121766634
1106	24-25	5.017562105329045
1106	26-27	7.665627304113542
1106	28-29	6.144303365832286
1106	30-31	5.335510821357023
1106	32-33	7.243119339555037
1106	34-35	6.637557324059308
1106	36-37	5.961727133536673
1106	38-39	7.09172734467494
1106	40-41	6.621820603131987
1106	42-43	6.068362385824429
1106	44-45	4.382207530100455
1106	46-47	5.881623322650931
1106	48-49	5.7136905194376375
1106	50-51	6.491876043327245
1106	52-53	5.538685115235218
1106	54-55	5.20236474311638
1106	56-57	4.959100780983949
1106	58-59	5.0300670585762965
1106	60-61	4.809579989316063
1106	62-63	4.975527473632145
1106	64-65	5.211517327642095
1106	66-67	2.9075172427724816
1106	68-69	3.2020205806011184
1106	70-71	2.6966151844509234
1106	72-73	2.6506619221474637
1106	74-75	2.6590302206277983
1106	76-77	3.4465014773393925
1106	78-79	4.118407914366173
1106	80-81	3.6677464839218743
1106	82-83	2.2522784777819993
1106	84-85	2.7499097976000755
1106	86-87	3.296055064793279
1106	88-89	3.3449408107104333
1106	90-91	3.2425400003119407
1106	92-93	2.960910336516946
1106	94-95	1.5659636477167709
1106	96-97	1.4859850681270323
1106	98-99	1.9187943327799388
1106	100	2.8407733877771797
1107	1	5.8567170712809435
1107	2	10.194412829743026
1107	3	5.928808981125783
1107	4	11.15644544527304
1107	5	9.22660866240728
1107	6	7.608166405588097
1107	7	3.5624977367848416
1107	8	5.9537365206453465
1107	9	5.574327456182061
1107	10-11	4.4619964574604225
1107	12-13	5.9673208595418
1107	14-15	5.875955431127441
1107	16-17	6.810275761712713
1107	18-19	6.064733489497897
1107	20-21	5.433258064195886
1107	22-23	5.530583478650488
1107	24-25	4.354098837972199
1107	26-27	7.120443211752971
1107	28-29	6.274644916803091
1107	30-31	4.837045653164285
1107	32-33	4.810797528090447
1107	34-35	6.564083365616064
1107	36-37	6.286986686090085
1107	38-39	6.160503429986093
1107	40-41	7.460615115159957
1107	42-43	5.759496395857443
1107	44-45	4.1103350928298745
1107	46-47	3.399067515715384
1107	48-49	3.952447203728898
1107	50-51	4.507315229859774
1107	52-53	3.75494433318692
1107	54-55	5.07320618295574
1107	56-57	5.067116366053465
1107	58-59	2.9197922767629656
1107	60-61	2.840086762349749
1107	62-63	0.5276162460806155
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2206	82-83	3.0099316330481116
2206	84-85	3.3927784652606796
2206	86-87	-1.3916544798080643
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2206	98-99	1.2283909109114752
2206	100	1.6067094953206542
2207	1	6.195051019763621
2207	2	10.662455696543603
2207	3	7.712309228308513
2207	4	11.938154647083703
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2207	6	9.181278028117486
2207	7	5.726465669660943
2207	8	6.649680989504439
2207	9	5.947795790464948
2207	10-11	6.7771005948825405
2207	12-13	8.616283266055945
2207	14-15	8.137648446898975
2207	16-17	7.189995348442164
2207	18-19	4.292304246021786
2207	20-21	7.425778915013559
2207	22-23	6.2828731942767995
2207	24-25	4.423860735247498
2207	26-27	3.8488427354052774
2207	28-29	5.158777326638791
2207	30-31	5.514337839370118
2207	32-33	6.768300004284917
2207	34-35	6.257183018312475
2207	36-37	6.652111636272966
2207	38-39	6.887394652366456
2207	40-41	4.152055736278623
2207	42-43	5.650049224209315
2207	44-45	3.910328610678068
2207	46-47	5.526247459001858
2207	48-49	2.6447320954863223
2207	50-51	5.343695236932117
2207	52-53	2.998781422674554
2207	54-55	3.218456637330249
2207	56-57	5.282162781754177
2207	58-59	3.758092847466834
2207	60-61	3.543925419033279
2207	62-63	4.282887813927847
2207	64-65	3.525419777204494
2207	66-67	3.5446573725478174
2207	68-69	3.5411851212505248
2207	70-71	3.6307373044609115
2207	72-73	3.8825503530823333
2207	74-75	3.596963883488616
2207	76-77	4.056518269448347
2207	78-79	4.287958154220803
2207	80-81	4.257906337155017
2207	82-83	3.850433983844731
2207	84-85	3.1856776959234807
2207	86-87	3.3741401058990483
2207	88-89	3.23328270627448
2207	90-91	1.3401911718751975
2207	92-93	-0.6335349734287412
2207	94-95	1.3398741859076004
2207	96-97	-0.8490736315522085
2207	98-99	0.8910280073146382
2207	100	2.1113259819336783
2208	1	1.2650014856109166
2208	2	8.42668242236035
2208	3	6.493930926368357
2208	4	11.762067117892489
2208	5	9.952557380670171
2208	6	9.094294197422474
2208	7	7.568618748778217
2208	8	7.7563399795895265
2208	9	6.484619867457017
2208	10-11	6.782152189591351
2208	12-13	7.011579784120155
2208	14-15	7.8844874730235865
2208	16-17	7.101543755349464
2208	18-19	6.501847166193421
2208	20-21	6.658630159023808
2208	22-23	5.937519950315039
2208	24-25	6.224352602111335
2208	26-27	7.997110817795196
2208	28-29	7.089157115411588
2208	30-31	6.060146062201074
2208	32-33	8.331800485081345
2208	34-35	6.555659944124372
2208	36-37	6.8418424306779535
2208	38-39	7.190151838050841
2208	40-41	7.632475160454195
2208	42-43	6.986531450902806
2208	44-45	5.967943779603264
2208	46-47	4.696104714987573
2208	48-49	4.634879849480168
2208	50-51	6.005627917351987
2208	52-53	5.669622331581813
2208	54-55	6.399632083582059
2208	56-57	6.543347601516121
2208	58-59	5.911593611084683
2208	60-61	5.1706184284956755
2208	62-63	3.524586075788168
2208	64-65	3.635832938905626
2208	66-67	3.0928630322085944
2208	68-69	5.505357262531948
2208	70-71	5.3925178269532985
2208	72-73	4.683671376411063
2208	74-75	2.2507662559563535
2208	76-77	3.5341548923989023
2208	78-79	4.32297451746048
2208	80-81	3.6223427144549625
2208	82-83	1.4536981471377395
2208	84-85	2.1028494412549072
2208	86-87	1.4215695419531258
2208	88-89	0.9487580078833311
2208	90-91	2.222613941424221
2208	92-93	1.0157060550715755
2208	94-95	-0.7408077773579365
2208	96-97	1.658786824355932
2208	98-99	0.4710585116256425
2208	100	2.0174773479611687
2209	1	5.254626887142916
2209	2	9.48543211348958
2209	3	5.563693713618171
2209	4	-3.3628145228290336
2209	5	0.6802702544038013
2209	6	4.415008800832407
2209	7	2.070781338350738
2209	8	5.056313293155132
2209	9	4.771803460241799
2209	10-11	5.007620931869585
2209	12-13	7.110374941026507
2209	14-15	6.605675019354205
2209	16-17	6.230201526538
2209	18-19	5.219570665081484
2209	20-21	5.997645479013922
2209	22-23	5.4451196537991
2209	24-25	5.2861121771051565
2209	26-27	6.865234860295814
2209	28-29	6.094611278989554
2209	30-31	4.060443072332038
2209	32-33	6.157701399338819
2209	34-35	5.639258659221976
2209	36-37	6.075247669126188
2209	38-39	6.658346550183278
2209	40-41	7.032514201719334
2209	42-43	6.649918898394777
2209	44-45	6.38011847690991
2209	46-47	6.627539605029579
2209	48-49	5.249987583604209
2209	50-51	4.747025248708546
2209	52-53	4.767303329852137
2209	54-55	5.34979368476812
2209	56-57	5.046392573100011
2209	58-59	3.2415466627278775
2209	60-61	4.123594954317269
2209	62-63	4.150503298422215
2209	64-65	4.900509489436882
2209	66-67	4.465386877031918
2209	68-69	3.8576584733053636
2209	70-71	3.617707350056829
2209	72-73	0.8204042649704881
2209	74-75	2.22361083218566
2209	76-77	3.422216913649212
2209	78-79	3.550098807057715
2209	80-81	3.6024378466516502
2209	82-83	2.9348515941355195
2209	84-85	2.951803975477791
2209	86-87	1.5151489835147736
2209	88-89	1.9512457766008993
2209	90-91	2.307834104507986
2209	92-93	2.2230648393562333
2209	94-95	0.746777347425855
2209	96-97	0.2691043435059193
2209	98-99	0.31561423191227433
2209	100	0.2027286452183965
2210	1	1.9670437525997286
2210	2	-16.829655605808664
2210	3	-7.439823110570815
2210	4	-5.2939164946259964
2210	5	0.2508449642708399
2210	6	3.2971223578172406
2210	7	-5.046961371838389
2210	8	-5.240690018336448
2210	9	-1.6204294916157558
2210	10-11	-0.37833065392220533
2210	12-13	3.625319410634262
2210	14-15	-2.7840383000889837
2210	16-17	0.06415866838861106
2210	18-19	-0.1538135585297553
2210	20-21	2.8335289628238485
2210	22-23	2.9596756421635853
2210	24-25	2.654808335171005
2210	26-27	4.31370217315628
2210	28-29	3.6593749552484667
2210	30-31	2.965164957365907
2210	32-33	4.714895911724199
2210	34-35	2.5073025440344097
2210	36-37	4.154789012444322
2210	38-39	3.4642257864265
2210	40-41	4.275382376171525
2210	42-43	2.8036158717518624
2210	44-45	3.686935036380877
2210	46-47	2.976429138099199
2210	48-49	3.088363765021608
2210	50-51	2.238990704516741
2210	52-53	2.5279456988267803
2210	54-55	2.8854340708739485
2210	56-57	3.1046431795357776
2210	58-59	2.144304360184371
2210	60-61	3.122334292622874
2210	62-63	2.3653605620632447
2210	64-65	2.908906103461902
2210	66-67	2.9718662078891818
2210	68-69	3.486968253780649
2210	70-71	2.6503156177139253
2210	72-73	1.1860500662206022
2210	74-75	0.45733393512372444
2210	76-77	-0.35947905317548035
2210	78-79	0.4855102674355649
2210	80-81	1.1960876337700483
2210	82-83	1.1470012051107474
2210	84-85	-0.311549307327617
2210	86-87	-0.09356404403401442
2210	88-89	-1.2350907877014237
2210	90-91	-1.1367881740800527
2210	92-93	-0.7403431585501821
2210	94-95	-0.06976436830926502
2210	96-97	0.190417666975236
2210	98-99	-0.047535771610590416
2210	100	0.1631045246760756
2211	1	1.2242900093303035
2211	2	-16.829655605808664
2211	3	-7.7899602506849845
2211	4	-18.829200487741314
2211	5	-20.96927360524532
2211	6	-12.803941466185032
2211	7	-11.020760074294913
2211	8	-18.904154010992695
2211	9	-15.22382990932399
2211	10-11	-9.57549690762589
2211	12-13	-22.011800497569986
2211	14-15	-17.49269187363403
2211	16-17	-15.613703218863364
2211	18-19	-18.735683576478717
2211	20-21	-8.22873216818699
2211	22-23	-3.9408267536742407
2211	24-25	-1.9254765162685068
2211	26-27	-17.080108156029794
2211	28-29	-3.9145984229066144
2211	30-31	-4.459701710457022
2211	32-33	-0.6059780117000031
2211	34-35	-5.050433280894701
2211	36-37	-5.994306335610286
2211	38-39	-2.260987948889902
2211	40-41	-5.012243634205408
2211	42-43	-1.330937363781164
2211	44-45	0.16051610972286312
2211	46-47	0.72703281275707
2211	48-49	0.6288390883013015
2211	50-51	-4.302974908823867
2211	52-53	-1.0115266244109513
2211	54-55	-0.8234016711763665
2211	56-57	-6.193654413567497
2211	58-59	-1.9180123515393106
2211	60-61	-0.8873602487157513
2211	62-63	-0.39684145075992916
2211	64-65	-3.2202456999433338
2211	66-67	-2.30945448257782
2211	68-69	0.1266228657291073
2211	70-71	-0.206726095124516
2211	72-73	-1.12348052632532
2211	74-75	-1.643219731202759
2211	76-77	-1.6307371446482826
2211	78-79	-1.068249161036718
2211	80-81	-0.8341243760256631
2211	82-83	-1.3955520984242256
2211	84-85	-1.6354644860081642
2211	86-87	-1.9920070628417612
2211	88-89	-2.5220004308062727
2211	90-91	-2.3863052486639305
2211	92-93	-2.3047836144631404
2211	94-95	-2.3471825028133324
2211	96-97	-3.1456349140963304
2211	98-99	-3.0221924548135206
2211	100	-2.560090302780157
2212	1	-20.971759077769367
2212	2	-16.829655605808664
2212	3	-19.78367470743446
2212	4	-18.829200487741314
2212	5	-20.96927360524532
2212	6	-21.54204383074654
2212	7	-22.11504322305277
2212	8	-22.55126565421329
2212	9	-23.90805618888101
2212	10-11	-11.162141932605632
2212	12-13	-22.011800497569986
2212	14-15	-23.39706182275106
2212	16-17	-23.77099145591814
2212	18-19	-18.423301132485324
2212	20-21	-23.502003643793095
2212	22-23	-18.51515666855947
2212	24-25	-24.99416852464923
2212	26-27	-17.07128145295361
2212	28-29	-17.946451197471198
2212	30-31	-6.155984882227354
2212	32-33	-17.071331612546864
2212	34-35	-13.584108150212993
2212	36-37	-17.55120430203987
2212	38-39	-23.514355204202683
2212	40-41	-10.225564329606488
2212	42-43	-13.971470876591866
2212	44-45	-16.996835635780702
2212	46-47	-16.92982431400415
2212	48-49	-17.589780378487742
2212	50-51	-9.061424707935434
2212	52-53	-2.230084973657508
2212	54-55	-23.384311578389774
2212	56-57	-23.28883549707543
2212	58-59	-12.346834099462171
2212	60-61	-6.324821088285489
2212	62-63	-3.322824744600968
2212	64-65	-17.26939309528253
2212	66-67	-17.571380883607823
2212	68-69	-14.62686057268705
2212	70-71	-9.648777624236118
2212	72-73	-17.508763422095402
2212	74-75	-6.588409698421941
2212	76-77	-10.929786624902611
2212	78-79	-4.715379782076127
2212	80-81	-2.5493064667028804
2212	82-83	-2.956021780013785
2212	84-85	-5.277030422048728
2212	86-87	-5.097805339386834
2212	88-89	-3.4188429849935495
2212	90-91	-4.209350014051019
2212	92-93	-5.511074372878049
2212	94-95	-3.8260367297040645
2212	96-97	-5.329866498476722
2212	98-99	-3.800566199195451
2212	100	-3.688289354098419
2213	1	-20.971759077769367
2213	2	-16.829655605808664
2213	3	-19.78367470743446
2213	4	-18.829200487741314
2213	5	-20.96927360524532
2213	6	-21.54204383074654
2213	7	-10.439162874699873
2213	8	-10.307418626572243
2213	9	-4.292380308532657
2213	10-11	-17.451062395153006
2213	12-13	-16.030732720212924
2213	14-15	-23.39706182275106
2213	16-17	-17.76455450021954
2213	18-19	-18.32754633605445
2213	20-21	-17.52434366651176
2213	22-23	-19.602691023974472
2213	24-25	-7.601324904808262
2213	26-27	-23.067537069528747
2213	28-29	-23.9764062648701
2213	30-31	-19.301673297729806
2213	32-33	-23.166438951538375
2213	34-35	-17.68871761239231
2213	36-37	-23.577664611575567
2213	38-39	-11.292280232601017
2213	40-41	-17.037079638997568
2213	42-43	-23.088046013885926
2213	44-45	-17.0360198545302
2213	46-47	-16.875053857042868
2213	48-49	-6.261816530998846
2213	50-51	-23.256132646028295
2213	52-53	-17.716245830819325
2213	54-55	-17.57552702708345
2213	56-57	-17.439346667086788
2213	58-59	-17.89126414126051
2213	60-61	-19.217879739611796
2213	62-63	-17.493011531818855
2213	64-65	-17.550130924547723
2213	66-67	-8.869897232522543
2213	68-69	-22.668797667045514
2213	70-71	-17.381576759497634
2213	72-73	-9.496149060789957
2213	74-75	-4.430339439176198
2213	76-77	-3.0560675745497043
2213	78-79	-4.926504499770505
2213	80-81	-3.4051224209237603
2213	82-83	-3.2121321241530083
2213	84-85	-3.0253733563409675
2213	86-87	-3.225101426683384
2213	88-89	-2.870482356681947
2213	90-91	-2.8117751344166635
2213	92-93	-3.66825208605081
2213	94-95	-3.6929586111756834
2213	96-97	-3.34894437541368
2213	98-99	-3.776127280141239
2213	100	-3.47449058991981
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	907.0
3	868.0
4	4894.0
5	16039.0
6	23874.0
7	30376.0
8	28009.0
9	28374.0
10	34962.0
11	43131.0
12	53861.0
13	71495.0
14	59250.0
15	62802.0
16	68906.0
17	71400.0
18	70128.0
19	69294.0
20	68265.0
21	65615.0
22	67339.0
23	72786.0
24	79013.0
25	86592.0
26	93728.0
27	101885.0
28	108995.0
29	114188.0
30	119128.0
31	127634.0
32	143270.0
33	172863.0
34	207935.0
35	245914.0
36	269730.0
37	233534.0
38	72715.0
39	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08145490370255	21.648385536381802	14.197673633213462	24.072485926702186
2	14.458279356726301	21.731097542784557	40.64576120987155	23.164861890617587
3	17.28578135479544	27.281857813547955	30.70741113346747	24.724949698189135
4	11.737542424263832	18.190227760169254	39.86228918771898	30.209940627847935
5	12.707661330264408	37.851715375070015	36.45045502599763	12.990168268667954
6	29.2502303673859	38.812157823868674	18.927019263764997	13.01059254498043
7	25.00229150078937	32.235259835668614	25.363698796927885	17.398749866614132
8	23.727107723804526	36.54795227567934	22.49972903458895	17.225210965927175
9	23.968658766731338	17.585770852467782	23.00828593283908	35.4372844479618
10-11	22.613910847699138	27.998762739213035	29.5955918956381	19.79173451744973
12-13	23.39123666172323	25.688099368305085	29.748153901056018	21.172510068915667
14-15	20.928401432642328	27.193314200258833	27.145701784974204	24.73258258212464
16-17	20.378716795686053	30.935624633411056	27.912327719204182	20.77333085169871
18-19	20.239085330088333	29.843425810009894	29.643469717182587	20.274019142719187
20-21	21.924821380318278	29.12633555631936	28.48299192645704	20.46585113690532
22-23	20.744129949395656	29.25785407702424	29.154084493516404	20.843931480063702
24-25	21.094072082638966	28.98318005396783	28.59808897807028	21.324658885322922
26-27	21.05743311189171	29.01619146618887	28.72422681087893	21.20214861104049
28-29	20.762802448252067	28.869870737436266	29.2312275568917	21.136099257419964
30-31	21.53518673190924	29.315557096442912	28.33504713276758	20.814209038880264
32-33	20.501390719918618	29.172095055158636	29.1803767443955	21.146137480527248
34-35	20.718549106262284	28.541363633823956	28.947728876956546	21.79235838295721
36-37	21.323171042259943	29.269664873076483	28.460491827994783	20.946672256668794
38-39	21.002111408383872	28.876451227135824	28.77432031981602	21.34711704466428
40-41	21.307063881842133	28.95070205956612	28.267749955776928	21.474484102814824
42-43	20.870066628617515	29.10380120450119	28.965522692104216	21.060609474777078
44-45	21.42941641041152	28.48448186049638	28.744416998538085	21.341684730554014
46-47	21.248657935909193	29.047612594095067	28.863919009516962	20.839810460478773
48-49	21.02772583509226	28.758896593417667	28.95895953530307	21.254418036187005
50-51	21.446248364288444	28.795624909127138	28.538421046963098	21.219705679621324
52-53	20.738827185789766	28.858592377498926	28.901167543312585	21.501412893398726
54-55	21.270626800804934	28.771349272006937	28.500116719603803	21.457907207584327
56-57	21.39511813105079	28.850836622383095	28.457586232672156	21.296459013893955
58-59	20.989764292217057	28.362585162357394	28.942082992868713	21.70556755255684
60-61	21.583875187700517	28.821571040824256	28.33842200272128	21.256131768753946
62-63	20.957634904120624	28.976522678731087	28.840548699757928	21.22529371739036
64-65	21.029564754474773	28.335003528163043	28.828402799602316	21.807028917759872
66-67	21.16419269729746	28.71296014911814	28.53856681973147	21.58428033385293
68-69	21.603388933015623	28.49301694466508	28.53206910246227	21.371525019857028
70-71	21.740722756514785	28.627999507178526	28.187761761079827	21.443515975226862
72-73	21.240802694237935	28.832759817165332	28.767124815655514	21.15931267294122
74-75	21.616330048486233	28.24611233547902	28.40207894351198	21.735478672522763
76-77	21.57229413223105	28.798114542674604	28.36738944328535	21.262201881808995
78-79	21.50585304596899	28.490746526375343	28.663529758850608	21.33987066880505
80-81	21.717196337694624	28.451515633559072	28.310320984106664	21.52096704463964
82-83	21.083339576081077	28.758015609081983	28.845793383698876	21.312851431138068
84-85	21.418421066421615	28.225569924856924	28.39652792962488	21.95948107909658
86-87	21.599251774486113	28.62258456330063	28.409466821649943	21.368696840563317
88-89	21.36383743944286	28.10572486996106	28.77053758373681	21.759900106859273
90-91	21.767372289495473	28.211510875013158	28.36162266540343	21.65949417008794
92-93	21.22048347244275	28.57026275594199	28.74044261543943	21.468811156175832
94-95	21.247729493615775	27.984244471507164	28.593549909037826	22.174476125839234
96-97	21.459336424437332	28.349307891776142	28.574336688555636	21.61701899523089
98-99	21.444377959335693	28.060859397228988	28.626297916936466	21.868464726498853
100	21.682061468434995	27.92743547526258	28.2494927142181	22.14101034208433
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	364.0
1	446.5
2	413.0
3	305.5
4	372.0
5	544.0
6	850.5
7	1278.5
8	1949.0
9	2824.5
10	3871.0
11	4966.0
12	6131.0
13	7312.5
14	8341.5
15	9435.0
16	10386.5
17	11321.5
18	12315.5
19	13397.5
20	14558.0
21	15899.5
22	17488.0
23	19546.5
24	22384.0
25	25685.5
26	30323.5
27	36961.5
28	45199.5
29	54454.5
30	65058.0
31	75864.0
32	86154.5
33	96618.5
34	105656.5
35	113159.5
36	119379.5
37	124198.0
38	127308.0
39	129041.0
40	129213.0
41	127433.0
42	123660.0
43	118278.5
44	112668.0
45	106289.5
46	99291.5
47	93231.5
48	88596.0
49	85197.5
50	82459.5
51	77030.0
52	68580.5
53	61215.0
54	55706.0
55	50417.0
56	45471.5
57	41044.5
58	36353.0
59	31755.5
60	27243.5
61	22690.5
62	18449.5
63	14561.0
64	11563.0
65	9326.0
66	7376.5
67	5727.5
68	4432.0
69	3343.5
70	2471.5
71	1869.0
72	1449.5
73	1097.5
74	798.0
75	582.0
76	415.0
77	281.5
78	193.0
79	137.5
80	102.0
81	68.5
82	46.0
83	47.0
84	38.5
85	25.0
86	15.5
87	11.0
88	7.5
89	4.0
90	4.0
91	3.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	2.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.101445109084622
2	25.392124990947888
3	6.516361360973811
4	24.562684980514838
5	12.967578858402382
6	10.014473975841595
7	8.339386362361017
8	8.890287764709644
9	5.247592137969562
10-11	7.112092274809294
12-13	10.308593190365855
14-15	7.4909800201450665
16-17	8.20365953020553
18-19	6.895625298014366
20-21	8.290120923688573
22-23	5.984927879347796
24-25	5.518434490760262
26-27	10.294689771943185
28-29	6.660897348883009
30-31	4.514269108109032
32-33	9.150972941471155
34-35	7.4213688801055095
36-37	7.0550365329169304
38-39	6.348877679072737
40-41	8.818983761998556
42-43	6.787626929432393
44-45	6.718564402295647
46-47	6.3157101104342095
48-49	4.1718092595199225
50-51	6.855435484219856
52-53	4.719089816709997
54-55	5.188968930640595
56-57	5.00750345704502
58-59	3.8743826929244296
60-61	4.371927571811078
62-63	3.1559035607485337
64-65	4.83685349496201
66-67	2.721746984118127
68-69	5.274928735183925
70-71	2.673798216412429
72-73	1.6319039407021556
74-75	0.8466351062725891
76-77	1.1830131889676767
78-79	1.815250372821654
80-81	2.2517267982982343
82-83	0.82110109431032
84-85	1.2896008386567073
86-87	0.3995782890989362
88-89	0.6795746400393998
90-91	0.23725156848428916
92-93	0.3132422928508215
94-95	0.5968751636042049
96-97	0.59361483549607
98-99	0.5084857876341398
100	0.381427039343069
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3189863.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.41369463239525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.61687503789395	94.11603580872195
2	2.0092181077380133	3.874322821786637
3	0.22072299881740065	0.6384215941898223
4	0.06489019479857992	0.25025213703787713
5	0.02614669434048079	0.1260449701894846
6	0.016924063001307197	0.09790268652884707
7	0.009876057716296002	0.06665310489816116
8	0.009639104137360757	0.07434733142634863
9	0.005973514733078042	0.051833576282139034
>10	0.018073449567826173	0.27444762108589554
>50	4.7867483328485285E-4	0.03219208901266554
>100	9.537406016096129E-4	0.18574073097519184
>500	1.6311558774783957E-4	0.09574339707469201
>1k	6.52462331116295E-5	0.11606213079030944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.1349308732067805E-5	0.0	0.0	0.0
3	0.0	3.1349308732067805E-5	0.0	6.269861746413561E-5	0.0
4	0.0	3.1349308732067805E-5	0.0	6.269861746413561E-5	0.0
5	0.0	3.1349308732067805E-5	0.0	6.269861746413561E-5	0.0
6	0.0	3.1349308732067805E-5	0.0	6.269861746413561E-5	0.0
7	0.0	3.1349308732067805E-5	0.0	6.269861746413561E-5	0.0
8	0.0	3.1349308732067805E-5	0.0	6.269861746413561E-5	0.0
9	3.1349308732067805E-5	3.1349308732067805E-5	0.0	6.269861746413561E-5	0.0
10-11	3.1349308732067805E-5	3.1349308732067805E-5	0.0	9.404792619620341E-5	0.0
12-13	3.1349308732067805E-5	3.1349308732067805E-5	0.0	9.404792619620341E-5	0.0
14-15	6.269861746413561E-5	3.1349308732067805E-5	0.0	1.0972258056223731E-4	0.0
16-17	6.269861746413561E-5	3.1349308732067805E-5	0.0	1.2539723492827122E-4	0.0
18-19	6.269861746413561E-5	3.1349308732067805E-5	0.0	1.2539723492827122E-4	0.0
20-21	6.269861746413561E-5	3.1349308732067805E-5	0.0	2.0377050675844075E-4	0.0
22-23	6.269861746413561E-5	3.1349308732067805E-5	0.0	2.978184329546442E-4	0.0
24-25	6.269861746413561E-5	6.269861746413561E-5	0.0	3.9186635915084756E-4	0.0
26-27	9.404792619620341E-5	6.269861746413561E-5	0.0	4.8591428534705094E-4	0.0
28-29	9.404792619620341E-5	6.269861746413561E-5	0.0	9.404792619620341E-4	0.0
30-31	9.404792619620341E-5	6.269861746413561E-5	0.0	0.0026333419334936955	0.0
32-33	9.404792619620341E-5	6.269861746413561E-5	0.0	0.007100618427813357	0.0
34-35	9.404792619620341E-5	6.269861746413561E-5	0.0	0.014655801832241699	0.0
36-37	1.2539723492827122E-4	6.269861746413561E-5	0.0	0.026866357583382107	0.0
38-39	1.2539723492827122E-4	6.269861746413561E-5	0.0	0.04343446724827994	0.0
40-41	1.4107188929430512E-4	6.269861746413561E-5	0.0	0.06536330870636137	0.0
42-43	1.5674654366033903E-4	6.269861746413561E-5	0.0	0.08782508841288794	0.0
44-45	1.5674654366033903E-4	6.269861746413561E-5	0.0	0.11431525429148524	0.0
46-47	1.8809585239240681E-4	6.269861746413561E-5	0.0	0.14544511786242859	0.0
48-49	1.8809585239240681E-4	6.269861746413561E-5	0.0	0.17728034087984343	0.0
50-51	1.8809585239240681E-4	7.83732718301695E-5	0.0	0.21369256297214018	0.0
52-53	1.8809585239240681E-4	9.404792619620341E-5	0.0	0.25258138045427025	0.0
54-55	1.8809585239240681E-4	9.404792619620341E-5	0.0	0.2967212071490217	0.0
56-57	1.8809585239240681E-4	9.404792619620341E-5	0.0	0.3475854605668018	0.0
58-59	1.8809585239240681E-4	1.4107188929430512E-4	0.0	0.40504874347268205	0.0
60-61	1.8809585239240681E-4	1.5674654366033903E-4	0.0	0.4636562761472828	0.0
62-63	1.8809585239240681E-4	1.5674654366033903E-4	0.0	0.5250382226446715	0.0
64-65	1.8809585239240681E-4	1.5674654366033903E-4	0.0	0.5960130576140731	0.0
66-67	1.8809585239240681E-4	1.7242119802637294E-4	0.0	0.6825841736776783	0.0
68-69	1.8809585239240681E-4	1.8809585239240681E-4	0.0	0.7798610786732847	0.0
70-71	1.8809585239240681E-4	1.8809585239240681E-4	0.0	0.8922013265146497	0.0
72-73	1.8809585239240681E-4	1.8809585239240681E-4	0.0	1.0142912093716878	0.0
74-75	1.8809585239240681E-4	2.1944516112447463E-4	0.0	1.1435130599652714	0.0
76-77	1.8809585239240681E-4	2.3511981549050853E-4	0.0	1.2840990349742292	0.0
78-79	1.8809585239240681E-4	2.5079446985654244E-4	0.0	1.445281505820156	0.0
80-81	1.8809585239240681E-4	2.5079446985654244E-4	0.0	1.629348972040492	0.0
82-83	1.8809585239240681E-4	2.5079446985654244E-4	0.0	1.8267398944719568	0.0
84-85	1.8809585239240681E-4	2.5079446985654244E-4	0.0	2.0327832261134726	0.0
86-87	1.8809585239240681E-4	2.5079446985654244E-4	0.0	2.2592976563570284	0.0
88	1.8809585239240681E-4	2.5079446985654244E-4	0.0	2.4502306211896876	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGC	125	0.0022311048	22.559002	4
GTGTAGG	1055	0.0	17.77663	1
GTACATG	10550	0.0	17.124819	1
TACATGG	10655	0.0	16.53995	2
GTATTAG	1235	0.0	16.198084	1
ACATGGG	10890	0.0	15.122195	3
GTATTAT	1915	0.0	15.016534	1
TATACTG	1710	0.0	14.9386425	5
GTACTAA	985	0.0	13.962625	1
GTATTAA	1805	0.0	13.853623	1
GTATATA	2330	0.0	13.683427	1
GTACTGT	1195	0.0	13.411034	6
ATAGCGC	295	0.0014316862	13.382458	3
GTGTATA	1875	0.0	13.336422	1
CTTACAC	1655	0.0	13.290046	3
CCTACAC	1065	0.0	13.238851	3
CCGTGCA	345	5.7490965E-5	13.080502	9
GTCTAGG	845	1.6189006E-10	12.576878	1
CATGGGG	3680	0.0	12.5668335	4
TCTGTCG	680	1.70985E-10	12.531721	8
>>END_MODULE