FastQCFastQC Report
Wed 25 May 2016
SRR1295159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189863
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT61900.1940522210514997No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42640.13367345243353712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106100.018.4760531
GAGTACT38700.017.58978512-13
ATCAACG118300.016.7730793
TCAACGC118250.016.6616674
CAACGCA116100.016.60575
AACGCAG116750.016.4502356
GTACATG135900.016.3699531
TATCAAC120400.016.2250692
TACACCG2701.044173E-515.6741615
TACATGG138000.015.4196962
ACATGGG133050.015.0903083
CTAATAC17000.014.935853
TAGACAG20300.014.8248225
CGCAGAG129000.014.7397218
ACGCAGA129700.014.6963687
CATGGGG44000.014.5342234
TATACCG1950.004773558614.4684565
CTAGACT13750.014.3632334
TCTGTCG5602.0008883E-1114.2522968
GTACTAA11700.014.0688981
Produced by FastQC (version 0.11.5)