FastQCFastQC Report
Wed 25 May 2016
SRR1295158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874483
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86950.017.1972471
GTATAAT14000.016.155711
TACATGG88050.016.0514052
TAAGTCG2150.002619888215.8330514
GTACTAG5651.0968506E-915.5679411
GTATATA21850.015.5272271
TATACCG2305.0268066E-415.3672585
GTATCAA62700.014.8301561
GTACAGG12300.014.8130481
GTATAAA18950.014.58797551
ACATGGG90150.014.5374323
GTGTATA14300.014.4987141
GTGTTAG11750.014.43701651
GTATTAA15700.014.0061871
TGTAGGA13600.013.8561392
GTATAGC6355.695256E-913.8517891
TATACTG13500.013.8386635
GTATAAG11000.013.7078741
GTACTAC6558.80209E-913.4288321
GTGTAGG9001.8189894E-1213.2636371
Produced by FastQC (version 0.11.5)