##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295158_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2874483
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.627550415152918	27.0	22.0	33.0	6.0	33.0
2	21.643885874433767	27.0	2.0	33.0	2.0	33.0
3	23.969074438777337	27.0	15.0	33.0	6.0	33.0
4	23.812703710545513	33.0	2.0	37.0	2.0	37.0
5	25.777299083000315	33.0	15.0	37.0	2.0	37.0
6	26.73720352494692	33.0	15.0	37.0	2.0	37.0
7	26.52169172682531	33.0	15.0	37.0	6.0	37.0
8	27.18345803401864	33.0	15.0	37.0	2.0	37.0
9	28.211832527797174	33.0	22.0	37.0	6.0	37.0
10-11	27.755856966278806	33.0	15.0	37.0	4.0	37.0
12-13	26.520560914780155	33.0	15.0	37.0	4.0	37.0
14-15	28.221089148900862	33.0	15.0	37.0	4.0	40.0
16-17	28.64634022883419	33.0	22.0	37.0	6.0	40.0
18-19	28.86596441864502	33.0	18.5	38.5	6.0	40.0
20-21	28.35117984694987	33.0	15.0	37.0	6.0	40.0
22-23	29.12431244157645	33.0	22.0	37.0	6.0	40.0
24-25	29.26695565776524	33.0	24.5	37.0	6.0	40.0
26-27	27.787215648866248	33.0	15.0	37.0	4.0	40.0
28-29	28.506885238145433	33.0	15.0	37.0	6.0	40.0
30-31	29.24697467335865	33.0	22.0	37.0	6.0	40.0
32-33	27.96882482867354	33.0	18.5	37.0	4.0	40.0
34-35	27.64024939441284	33.0	15.0	37.0	6.0	40.0
36-37	27.912689864577388	33.0	18.5	37.0	6.0	40.0
38-39	28.034427234393107	33.0	18.5	37.0	6.0	40.0
40-41	27.145690894675667	33.0	15.0	37.0	4.0	40.0
42-43	27.739550207811284	33.0	15.0	37.0	6.0	40.0
44-45	27.637711198848628	33.0	18.5	37.0	6.0	40.0
46-47	27.357183013432326	33.0	15.0	37.0	6.0	40.0
48-49	27.31824488090554	33.0	15.0	37.0	6.0	40.0
50-51	27.18734534175363	33.0	15.0	37.0	6.0	40.0
52-53	27.449362372294427	33.0	18.5	37.0	6.0	40.0
54-55	27.395001466350646	33.0	22.0	37.0	6.0	40.0
56-57	27.383099673923972	33.0	22.0	37.0	6.0	40.0
58-59	27.266127856731107	33.0	22.0	37.0	6.0	40.0
60-61	27.231762894405705	33.0	22.0	37.0	6.0	40.0
62-63	27.282350774034843	33.0	22.0	37.0	6.0	40.0
64-65	26.825735445295727	33.0	18.5	37.0	6.0	40.0
66-67	26.9933174069911	33.0	18.5	37.0	6.0	38.5
68-69	26.547462969862757	30.0	18.5	37.0	6.0	37.0
70-71	26.725791559734397	27.0	18.5	37.0	6.0	37.0
72-73	26.79633885467404	27.0	22.0	37.0	6.0	37.0
74-75	26.92993766183345	27.0	22.0	37.0	6.0	37.0
76-77	26.678561675264735	27.0	22.0	35.0	6.0	37.0
78-79	26.248893801076576	27.0	22.0	33.0	6.0	37.0
80-81	26.084231146957556	27.0	22.0	33.0	6.0	37.0
82-83	26.17945627091898	27.0	22.0	33.0	6.0	37.0
84-85	26.125027352744823	27.0	22.0	33.0	6.0	37.0
86-87	26.005267381995303	27.0	22.0	33.0	6.0	37.0
88-89	25.97063576302243	27.0	22.0	33.0	6.0	37.0
90-91	26.12106716233841	27.0	22.0	33.0	6.0	37.0
92-93	26.0042861620681	27.0	22.0	33.0	6.0	37.0
94-95	25.873916457324675	27.0	22.0	33.0	6.0	37.0
96-97	25.954288649471923	27.0	22.0	33.0	6.0	37.0
98-99	26.068976751645426	27.0	22.0	33.0	6.0	37.0
100	25.70499460250765	27.0	22.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.238344926043673
1101	2	11.236187017249236
1101	3	8.464700507327617
1101	4	12.771246003213452
1101	5	10.724463995911481
1101	6	9.941313639802747
1101	7	9.377475323366202
1101	8	8.917396455762596
1101	9	7.410694668592146
1101	10-11	7.780929734897889
1101	12-13	9.219380170959727
1101	14-15	8.183283858459667
1101	16-17	8.33133069016333
1101	18-19	8.17182077404134
1101	20-21	7.2772662355102185
1101	22-23	7.408653744067109
1101	24-25	7.441925028040341
1101	26-27	8.587747530744533
1101	28-29	8.193168662458493
1101	30-31	5.620839130626035
1101	32-33	7.028792333917888
1101	34-35	8.701335952490759
1101	36-37	8.255055619227804
1101	38-39	8.255404979866007
1101	40-41	8.973591040340857
1101	42-43	8.66733121338045
1101	44-45	8.57743528051343
1101	46-47	7.378295450016761
1101	48-49	5.512042111847517
1101	50-51	6.852369228840129
1101	52-53	6.065624856714834
1101	54-55	4.316956853466284
1101	56-57	6.219595972361258
1101	58-59	6.3330615973240825
1101	60-61	6.770565504611877
1101	62-63	6.216208944743823
1101	64-65	6.850549457459447
1101	66-67	5.986467875578057
1101	68-69	6.927486013499493
1101	70-71	6.510282669338842
1101	72-73	5.729911293642477
1101	74-75	5.188990570744483
1101	76-77	5.258074022725076
1101	78-79	5.258413758461128
1101	80-81	5.120090961972025
1101	82-83	4.712188122924008
1101	84-85	4.495687118468549
1101	86-87	4.277568323963742
1101	88-89	4.283089456008792
1101	90-91	3.9526964568153424
1101	92-93	3.8986905848345828
1101	94-95	3.9069632169815627
1101	96-97	4.066563047344001
1101	98-99	3.703175582029562
1101	100	3.943074111184135
1102	1	6.701059108618484
1102	2	10.425655271591186
1102	3	8.115861078675469
1102	4	12.364473999751556
1102	5	10.337799677910052
1102	6	9.343675645910398
1102	7	6.526473609232951
1102	8	7.700275414861064
1102	9	6.371824623210781
1102	10-11	7.266934660928953
1102	12-13	8.942987549804482
1102	14-15	6.2361007573022675
1102	16-17	7.192017453332678
1102	18-19	7.301410214464841
1102	20-21	6.780339007137002
1102	22-23	6.412793476756498
1102	24-25	6.830767018363172
1102	26-27	8.361662155922229
1102	28-29	7.559580297151921
1102	30-31	4.254539197666698
1102	32-33	4.460403238002659
1102	34-35	4.890856196976163
1102	36-37	5.095756880780549
1102	38-39	4.714530833569391
1102	40-41	6.184944874887201
1102	42-43	6.9269518282711005
1102	44-45	6.530809188580289
1102	46-47	5.535361807377104
1102	48-49	3.63580844600148
1102	50-51	4.932546171898167
1102	52-53	5.897340035370345
1102	54-55	5.423132946184186
1102	56-57	6.314491675368895
1102	58-59	6.0531317826163935
1102	60-61	6.0593080550269
1102	62-63	4.171631194395168
1102	64-65	5.980708645393026
1102	66-67	3.831599341974769
1102	68-69	6.1716674616225795
1102	70-71	5.36570791788256
1102	72-73	5.028085164377327
1102	74-75	4.347114976571415
1102	76-77	3.536625818360932
1102	78-79	4.33576685197276
1102	80-81	4.470675185443881
1102	82-83	3.949823409405113
1102	84-85	3.087600871445023
1102	86-87	3.3010284067443827
1102	88-89	3.4034951528953243
1102	90-91	3.3319181545583803
1102	92-93	3.326902090267339
1102	94-95	3.220810532440378
1102	96-97	3.4313476568041317
1102	98-99	3.154540372342524
1102	100	3.4444957823575493
1103	1	6.530581772801568
1103	2	10.72412245531891
1103	3	7.776033117140834
1103	4	11.604346776509576
1103	5	10.14024462243638
1103	6	8.951170921379916
1103	7	7.891815165660574
1103	8	7.865900047523166
1103	9	7.00073756299901
1103	10-11	7.258448251300855
1103	12-13	8.547107583396116
1103	14-15	8.405916574080912
1103	16-17	7.8525541735590885
1103	18-19	7.01365954873555
1103	20-21	8.128979291784312
1103	22-23	6.68920478895528
1103	24-25	5.075840169466769
1103	26-27	5.865775775229057
1103	28-29	6.903657256136672
1103	30-31	5.999219071166824
1103	32-33	7.786385895132856
1103	34-35	6.89408402333045
1103	36-37	4.085150112042925
1103	38-39	6.202500291401261
1103	40-41	6.746084763939855
1103	42-43	7.315658957112888
1103	44-45	5.554045316771042
1103	46-47	6.205596502384925
1103	48-49	5.560081399533804
1103	50-51	6.328069424952158
1103	52-53	6.042305612983757
1103	54-55	4.741079041325829
1103	56-57	5.686382047047029
1103	58-59	5.383423765382027
1103	60-61	5.072856832978488
1103	62-63	2.780592035045121
1103	64-65	4.929217310046301
1103	66-67	4.1002890137894425
1103	68-69	5.252938145571836
1103	70-71	3.7528203481448728
1103	72-73	4.385876118596851
1103	74-75	3.280030345310845
1103	76-77	3.5112543832339327
1103	78-79	2.9297860258806914
1103	80-81	4.129934643547017
1103	82-83	3.6222098285274775
1103	84-85	3.3947927823254567
1103	86-87	2.8744934994717717
1103	88-89	3.0673057014833915
1103	90-91	2.758650265767951
1103	92-93	2.4703115962939393
1103	94-95	2.9704582271407673
1103	96-97	3.004761237455714
1103	98-99	2.804490838240003
1103	100	3.029250524972362
1104	1	6.3575948896825665
1104	2	9.716781185240684
1104	3	7.185463109952028
1104	4	11.922820081682541
1104	5	9.224546448265311
1104	6	7.932996570608683
1104	7	3.765026430922564
1104	8	6.4595266472255055
1104	9	6.343787269819654
1104	10-11	7.012615011090432
1104	12-13	7.170025731854452
1104	14-15	7.179130992726371
1104	16-17	7.041390647281343
1104	18-19	6.557393025105672
1104	20-21	7.3041661361528085
1104	22-23	6.30544497259195
1104	24-25	5.864444563833171
1104	26-27	7.989668700202355
1104	28-29	6.923813575241329
1104	30-31	6.311983316313647
1104	32-33	7.596116309564788
1104	34-35	7.88830222791276
1104	36-37	6.558891654705416
1104	38-39	7.178209016469275
1104	40-41	7.066226746381339
1104	42-43	7.304550417426299
1104	44-45	6.548535582426961
1104	46-47	6.519370285194544
1104	48-49	5.417434529632285
1104	50-51	5.49214745950653
1104	52-53	5.904132381962217
1104	54-55	4.683797148121972
1104	56-57	6.005540566240899
1104	58-59	3.987738913332013
1104	60-61	4.297094898474118
1104	62-63	3.805766626997581
1104	64-65	2.7680800970333443
1104	66-67	3.2762560859829293
1104	68-69	4.235522000123346
1104	70-71	2.720446146677393
1104	72-73	2.6127231042039405
1104	74-75	3.1848258596242793
1104	76-77	3.7115052378747855
1104	78-79	4.254768636776429
1104	80-81	3.16450380179608
1104	82-83	3.088918046495035
1104	84-85	2.97703228398964
1104	86-87	0.7671553827770161
1104	88-89	0.5217256475409116
1104	90-91	2.338152750002884
1104	92-93	2.4557840382530607
1104	94-95	2.500414900959843
1104	96-97	2.8690171069146224
1104	98-99	2.681004867498057
1104	100	0.9618498109144085
1105	1	5.696256819312925
1105	2	-2.844936451183692
1105	3	0.49045427528135477
1105	4	8.1064166041083
1105	5	8.384107261461104
1105	6	7.821705036500521
1105	7	5.02035457870133
1105	8	5.781777250923966
1105	9	4.748039769710939
1105	10-11	4.821060991565169
1105	12-13	5.109077140505079
1105	14-15	5.764583517894156
1105	16-17	5.98376688621337
1105	18-19	5.907313702871161
1105	20-21	6.897495781099771
1105	22-23	2.174506160079794
1105	24-25	3.862246667722701
1105	26-27	6.722279568862053
1105	28-29	5.860760564682813
1105	30-31	5.225890079248561
1105	32-33	6.999263475355104
1105	34-35	6.036519715243969
1105	36-37	6.199208172476837
1105	38-39	4.072005630153544
1105	40-41	5.171488045004882
1105	42-43	5.47864814890066
1105	44-45	5.971604946958031
1105	46-47	5.435880831909259
1105	48-49	4.928027676666222
1105	50-51	5.822114869742435
1105	52-53	5.541526397452817
1105	54-55	5.247541546022088
1105	56-57	5.054549131572795
1105	58-59	1.9637787117955305
1105	60-61	4.362500223573395
1105	62-63	-0.6676547503282428
1105	64-65	-0.4102536972058992
1105	66-67	0.9283769921538472
1105	68-69	2.912827164003801
1105	70-71	3.0401414777617575
1105	72-73	2.732312297357179
1105	74-75	2.6838575015767354
1105	76-77	1.9072639096478952
1105	78-79	3.189552458235749
1105	80-81	1.702033175826621
1105	82-83	0.9682094229525866
1105	84-85	1.333729631298862
1105	86-87	-0.26716865193265704
1105	88-89	1.5032698899376093
1105	90-91	1.338439559633251
1105	92-93	1.6093392203946166
1105	94-95	1.0095604588620226
1105	96-97	1.932114413991041
1105	98-99	1.8311563086214058
1105	100	2.0195113187097817
1106	1	6.052218689950479
1106	2	10.090381685671048
1106	3	6.56096691063475
1106	4	11.525081390962395
1106	5	9.708763098226921
1106	6	9.112094528984453
1106	7	6.365702675832708
1106	8	7.269708707570221
1106	9	6.630233380110912
1106	10-11	5.451350658491499
1106	12-13	7.675692179168589
1106	14-15	7.732292912653904
1106	16-17	7.567946731417127
1106	18-19	6.9537573809283835
1106	20-21	8.075240971435512
1106	22-23	5.975580235663941
1106	24-25	4.854957820571215
1106	26-27	7.441732859915344
1106	28-29	6.056475907948393
1106	30-31	5.307989713832729
1106	32-33	7.094493047307989
1106	34-35	6.573345981190084
1106	36-37	5.875870374579389
1106	38-39	6.88950887859054
1106	40-41	6.522876357430754
1106	42-43	6.185885264359708
1106	44-45	4.554380098681367
1106	46-47	5.888139112201824
1106	48-49	5.791160980233325
1106	50-51	6.4979561027175805
1106	52-53	5.5631787603685545
1106	54-55	5.3120425454386115
1106	56-57	4.8088064559983295
1106	58-59	4.999167788781708
1106	60-61	4.73569032257296
1106	62-63	4.883378900323908
1106	64-65	5.153324513914065
1106	66-67	3.1452752775257053
1106	68-69	3.4495950908433137
1106	70-71	3.006033389242514
1106	72-73	2.876050968377612
1106	74-75	2.806362279245441
1106	76-77	3.4058689092635035
1106	78-79	4.087731150488782
1106	80-81	3.690919916248955
1106	82-83	2.1231001675956627
1106	84-85	2.743999627628259
1106	86-87	3.415108269100106
1106	88-89	3.316871111745421
1106	90-91	2.9947123064689123
1106	92-93	3.0288103811842504
1106	94-95	1.5426718665861934
1106	96-97	1.5033974005133608
1106	98-99	2.027634545759973
1106	100	2.870563511227001
1107	1	5.855812933599545
1107	2	10.062990947580658
1107	3	5.909363233799578
1107	4	10.909619535069591
1107	5	9.182735164275503
1107	6	7.803622501872351
1107	7	3.692153538000664
1107	8	6.056600138157922
1107	9	5.426236715888958
1107	10-11	4.434237348838291
1107	12-13	6.067993475555241
1107	14-15	6.091187166796022
1107	16-17	6.90474372455331
1107	18-19	6.165162397046334
1107	20-21	5.3923356405260705
1107	22-23	5.585263503384546
1107	24-25	4.4435613726776815
1107	26-27	6.928168131544556
1107	28-29	6.224515108289982
1107	30-31	4.904759669216368
1107	32-33	4.897912035571171
1107	34-35	6.558407738399822
1107	36-37	6.31455169514625
1107	38-39	6.240189502480469
1107	40-41	7.482251782267298
1107	42-43	6.1011124729927815
1107	44-45	4.03596446566447
1107	46-47	3.3958358029973716
1107	48-49	3.898301525621161
1107	50-51	4.6586508691273245
1107	52-53	3.7555565300828384
1107	54-55	5.111523573915914
1107	56-57	4.9706736062838885
1107	58-59	2.9410697278181885
1107	60-61	2.791108407377923
1107	62-63	0.7134137330708477
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2103	9	6.7580776286874205
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2103	78-79	3.820803474262263
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2104	4	12.091344889271571
2104	5	10.184371857828165
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2206	100	1.5370063756250616
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2207	3	7.569329091995176
2207	4	11.781243441944728
2207	5	10.221362567722945
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2207	9	5.913434836748959
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2207	12-13	8.53677487934392
2207	14-15	8.131959380756236
2207	16-17	7.1582279434838405
2207	18-19	3.9462971687267974
2207	20-21	7.310196912630495
2207	22-23	6.270986374673967
2207	24-25	4.370856080697994
2207	26-27	3.9113820192014117
2207	28-29	4.97834318079828
2207	30-31	5.365420703357525
2207	32-33	6.670462172426003
2207	34-35	6.111020755276314
2207	36-37	6.599783515792353
2207	38-39	6.770193251202151
2207	40-41	4.147075543691599
2207	42-43	5.447939618538555
2207	44-45	3.8745341921040186
2207	46-47	5.410713630027654
2207	48-49	2.4370846978073963
2207	50-51	5.262884956739068
2207	52-53	2.8957005087837864
2207	54-55	3.134163549799535
2207	56-57	5.131584970587191
2207	58-59	3.673326512874681
2207	60-61	3.5342547585752975
2207	62-63	4.099202417835034
2207	64-65	3.3047204587056846
2207	66-67	3.4165839476691175
2207	68-69	3.4475790071315835
2207	70-71	3.6224941858976436
2207	72-73	3.8622151548632466
2207	74-75	3.5283970205123545
2207	76-77	4.072199327822155
2207	78-79	4.285442228131185
2207	80-81	4.245748963589691
2207	82-83	3.8585941994259016
2207	84-85	3.3044385859522265
2207	86-87	3.4255104180907985
2207	88-89	3.3026798493257843
2207	90-91	1.5368365140847935
2207	92-93	-0.3812245174355411
2207	94-95	1.5540560661096414
2207	96-97	-0.6912157924012661
2207	98-99	0.8430835072309968
2207	100	2.1823554232892235
2208	1	1.125600571672262
2208	2	8.416622120947384
2208	3	6.490491067100539
2208	4	11.528671565535017
2208	5	9.852197438857285
2208	6	8.84102896848108
2208	7	7.278769149621191
2208	8	7.557362339976489
2208	9	6.373450471204965
2208	10-11	6.524019029551319
2208	12-13	6.848207160717113
2208	14-15	7.608080546320885
2208	16-17	7.101313134056074
2208	18-19	6.217515726372657
2208	20-21	6.414171201108463
2208	22-23	5.642477923923032
2208	24-25	5.995801699639198
2208	26-27	7.846226649170912
2208	28-29	6.982875701012549
2208	30-31	5.963640176966724
2208	32-33	8.177622904940566
2208	34-35	6.198163089281444
2208	36-37	6.632783752786189
2208	38-39	7.00849813547925
2208	40-41	7.299599155435661
2208	42-43	6.67145030960544
2208	44-45	5.713237255936807
2208	46-47	4.398110698294264
2208	48-49	4.5107116528228595
2208	50-51	5.687198540516182
2208	52-53	5.3274218948219705
2208	54-55	6.035880327682573
2208	56-57	6.236457838823448
2208	58-59	5.435404814987034
2208	60-61	5.045801503877453
2208	62-63	3.3228883090609536
2208	64-65	3.3819261072196873
2208	66-67	2.975613898211087
2208	68-69	5.2810071738593685
2208	70-71	5.167110072734239
2208	72-73	4.464742119996604
2208	74-75	2.181405666693994
2208	76-77	3.3645712383220285
2208	78-79	4.084284684327734
2208	80-81	3.530273103479452
2208	82-83	1.3255626982431359
2208	84-85	1.9082317704167657
2208	86-87	1.1069656714926275
2208	88-89	0.622208899648033
2208	90-91	1.9665231301465909
2208	92-93	0.7393249603278704
2208	94-95	-1.1927018388102795
2208	96-97	1.3282827887844455
2208	98-99	0.3294872979148522
2208	100	1.7628247225116773
2209	1	5.141340604171543
2209	2	9.303408164260212
2209	3	5.413588498587394
2209	4	-3.234649564707471
2209	5	0.5458403263860063
2209	6	4.297736176089142
2209	7	2.3276689939147595
2209	8	5.064628665875659
2209	9	4.83328250681323
2209	10-11	4.827211191453088
2209	12-13	7.051567065822095
2209	14-15	6.680011311226799
2209	16-17	6.171504328386643
2209	18-19	5.238801469537808
2209	20-21	5.848636218085968
2209	22-23	5.170019212875999
2209	24-25	4.99880734303289
2209	26-27	6.718236501833633
2209	28-29	6.026087129463466
2209	30-31	3.9088075035124774
2209	32-33	6.181347025240374
2209	34-35	5.4475093357379265
2209	36-37	6.1477496383144405
2209	38-39	6.607677474619386
2209	40-41	6.9618288874940575
2209	42-43	6.591433522425319
2209	44-45	6.304079253773274
2209	46-47	6.469184717747535
2209	48-49	4.934974644948031
2209	50-51	4.400354705271795
2209	52-53	4.800277227754421
2209	54-55	5.253191878918837
2209	56-57	5.011733655767824
2209	58-59	3.090583751358295
2209	60-61	4.084765701171445
2209	62-63	4.139244634512604
2209	64-65	4.800805679327919
2209	66-67	4.492005125154115
2209	68-69	3.705947208621975
2209	70-71	3.602960745790341
2209	72-73	0.807255788675743
2209	74-75	2.3516350417644
2209	76-77	3.262297986135735
2209	78-79	3.441404318512781
2209	80-81	3.6317995486249544
2209	82-83	3.0264947892514122
2209	84-85	3.0898502454196333
2209	86-87	1.6045600534070168
2209	88-89	1.9968636012158498
2209	90-91	2.334307574729351
2209	92-93	2.3284077892570636
2209	94-95	0.8625394418609815
2209	96-97	0.27814332427671573
2209	98-99	0.47519786090137117
2209	100	0.31964095548594784
2210	1	1.4386621004383429
2210	2	-16.863364998679085
2210	3	-7.423716457513512
2210	4	-8.065415089776089
2210	5	-1.727102621706102
2210	6	2.3118675782589584
2210	7	-8.513181016641553
2210	8	-8.617761598055466
2210	9	-3.7321447555326976
2210	10-11	-1.4633739198314366
2210	12-13	2.9877721242200757
2210	14-15	-5.5319156880861655
2210	16-17	-1.7731640342885662
2210	18-19	-0.8360560202529008
2210	20-21	1.908186268417996
2210	22-23	2.058722092941135
2210	24-25	1.4676943345073532
2210	26-27	3.494118887023536
2210	28-29	2.971713812786941
2210	30-31	2.303337030274772
2210	32-33	4.208825921365101
2210	34-35	1.8959870397576069
2210	36-37	3.5869001701542587
2210	38-39	2.7081731546966523
2210	40-41	3.757009114647225
2210	42-43	1.7718218488941346
2210	44-45	2.7884722425159296
2210	46-47	2.395620871890685
2210	48-49	2.3864812231049832
2210	50-51	1.8049492670798735
2210	52-53	1.9823066524529231
2210	54-55	2.480376539722961
2210	56-57	2.424266184955151
2210	58-59	1.3876089418676472
2210	60-61	2.4625712305792327
2210	62-63	1.3289261605003766
2210	64-65	2.2971289334247587
2210	66-67	2.441706632315494
2210	68-69	2.793652021483645
2210	70-71	2.031924874159756
2210	72-73	0.6356078842494419
2210	74-75	0.06524489797693533
2210	76-77	-0.49792513113730763
2210	78-79	0.0311798306688722
2210	80-81	0.5056003282756478
2210	82-83	0.485273995533408
2210	84-85	-1.41773965528105
2210	86-87	-1.2193605199698467
2210	88-89	-1.602461844627939
2210	90-91	-1.9695322107850544
2210	92-93	-1.4318334662091807
2210	94-95	-0.5405329989332763
2210	96-97	-0.1847938440888761
2210	98-99	-0.5334955243153594
2210	100	-0.4458348892231214
2211	1	1.0971110750857989
2211	2	-16.863364998679085
2211	3	-7.771237528615329
2211	4	-18.60174221646466
2211	5	-20.817098970738595
2211	6	-13.173921627097366
2211	7	-11.121476099949147
2211	8	-18.55439030867155
2211	9	-15.42179695409886
2211	10-11	-9.899051379155342
2211	12-13	-21.8858131258712
2211	14-15	-17.284979170846672
2211	16-17	-15.364530626809646
2211	18-19	-18.630971566909682
2211	20-21	-8.413051450359603
2211	22-23	-4.184284845493373
2211	24-25	-2.255809569154632
2211	26-27	-16.960829375565538
2211	28-29	-4.063881235944205
2211	30-31	-4.751972489208423
2211	32-33	-0.9727040495131796
2211	34-35	-5.725172760083886
2211	36-37	-6.671167075754319
2211	38-39	-2.703526750672612
2211	40-41	-5.510477574219664
2211	42-43	-1.9171209516985712
2211	44-45	-0.1727946680392698
2211	46-47	0.59289281345605
2211	48-49	0.3879480172584344
2211	50-51	-4.736352868172997
2211	52-53	-1.0117727853991774
2211	54-55	-0.4562599304184296
2211	56-57	-6.730959638071091
2211	58-59	-2.388063713644989
2211	60-61	-1.2618047993869048
2211	62-63	-0.6119483850020586
2211	64-65	-3.8380713062248546
2211	66-67	-2.6416173674174033
2211	68-69	0.08542465001087152
2211	70-71	-0.3368649671229207
2211	72-73	-1.1660192083947685
2211	74-75	-1.8593960333150825
2211	76-77	-1.8509919571447178
2211	78-79	-1.1874298965124304
2211	80-81	-1.0611760625128426
2211	82-83	-1.508578265587829
2211	84-85	-1.5438181295331255
2211	86-87	-2.0382787836133325
2211	88-89	-2.548948899123662
2211	90-91	-2.3659649840757595
2211	92-93	-2.1273374996991166
2211	94-95	-2.297221244174711
2211	96-97	-2.9568036267662983
2211	98-99	-2.805114736829072
2211	100	-2.2875149887365964
2212	1	-20.904346296786922
2212	2	-16.863364998679085
2212	3	-19.599510542946152
2212	4	-18.60174221646466
2212	5	-20.817098970738595
2212	6	-21.628621651386897
2212	7	-22.02504666103732
2212	8	-22.453102963517313
2212	9	-23.7378037551676
2212	10-11	-11.06085045935617
2212	12-13	-21.8858131258712
2212	14-15	-23.19219316161665
2212	16-17	-23.615077141261917
2212	18-19	-18.323812721383092
2212	20-21	-23.34601234421435
2212	22-23	-18.390127892414874
2212	24-25	-24.87531406271791
2212	26-27	-17.03665904989898
2212	28-29	-17.85956378511716
2212	30-31	-6.376301684424188
2212	32-33	-16.993163735564316
2212	34-35	-13.709281908161943
2212	36-37	-17.41512242572376
2212	38-39	-23.36165159886304
2212	40-41	-9.99211890592658
2212	42-43	-13.771479267659995
2212	44-45	-16.921830587184488
2212	46-47	-16.852464336074043
2212	48-49	-17.48005396861093
2212	50-51	-8.945252499025504
2212	52-53	-2.1774336875283815
2212	54-55	-23.21166435111311
2212	56-57	-23.15687656787435
2212	58-59	-12.353571885337274
2212	60-61	-6.4030738873550455
2212	62-63	-3.444242357728381
2212	64-65	-17.173270787336406
2212	66-67	-17.322568129067704
2212	68-69	-14.462760441555323
2212	70-71	-9.556488399313757
2212	72-73	-17.224987303695144
2212	74-75	-6.349824249742813
2212	76-77	-10.790526505666392
2212	78-79	-4.816600982895078
2212	80-81	-2.344605851087156
2212	82-83	-3.025059646727943
2212	84-85	-5.355046886202736
2212	86-87	-5.123912193196215
2212	88-89	-3.435907876507706
2212	90-91	-4.329492411227186
2212	92-93	-5.362369516504266
2212	94-95	-3.859836185706339
2212	96-97	-5.5971326298011554
2212	98-99	-3.880299804372651
2212	100	-3.962724666112898
2213	1	-20.904346296786922
2213	2	-16.863364998679085
2213	3	-19.599510542946152
2213	4	-18.60174221646466
2213	5	-20.817098970738595
2213	6	-21.628621651386897
2213	7	-10.415758207692624
2213	8	-10.25881237979341
2213	9	-4.675596682308633
2213	10-11	-17.352028161074987
2213	12-13	-15.989136517434897
2213	14-15	-23.19219316161665
2213	16-17	-17.70902686431262
2213	18-19	-18.17467559519441
2213	20-21	-17.4474183944913
2213	22-23	-19.510451335482244
2213	24-25	-7.671862848401762
2213	26-27	-22.964351357591287
2213	28-29	-23.812970378523755
2213	30-31	-19.188596734921198
2213	32-33	-23.00986703226761
2213	34-35	-17.633502961927096
2213	36-37	-23.394023524624856
2213	38-39	-11.312013763328316
2213	40-41	-16.79855555406753
2213	42-43	-22.949061685242413
2213	44-45	-16.940383802184233
2213	46-47	-16.799143894266166
2213	48-49	-6.338077351278542
2213	50-51	-23.11162612539913
2213	52-53	-17.572393069858872
2213	54-55	-17.426619613149754
2213	56-57	-17.320063615168767
2213	58-59	-17.867012752097274
2213	60-61	-19.002044563036414
2213	62-63	-17.394234444891328
2213	64-65	-17.496535090140547
2213	66-67	-8.602249180866352
2213	68-69	-22.40012307891796
2213	70-71	-17.259465967123024
2213	72-73	-9.292962694303682
2213	74-75	-4.476456049983476
2213	76-77	-3.417078990250406
2213	78-79	-4.826454759284854
2213	80-81	-3.5134095144029622
2213	82-83	-3.64673379703439
2213	84-85	-3.2144946731835446
2213	86-87	-3.371233768084899
2213	88-89	-2.9307653752084093
2213	90-91	-2.9751047196686358
2213	92-93	-3.817586417200502
2213	94-95	-4.081152151376802
2213	96-97	-3.7797625946381928
2213	98-99	-3.970851408712072
2213	100	-3.570952705648992
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	777.0
3	1113.0
4	9568.0
5	24315.0
6	24733.0
7	29034.0
8	27740.0
9	29006.0
10	32896.0
11	40551.0
12	50936.0
13	66944.0
14	56647.0
15	60172.0
16	65592.0
17	66551.0
18	65352.0
19	63441.0
20	62249.0
21	60106.0
22	61907.0
23	66945.0
24	71683.0
25	78540.0
26	83500.0
27	89571.0
28	95559.0
29	99276.0
30	102794.0
31	112374.0
32	125274.0
33	150909.0
34	180548.0
35	211642.0
36	233194.0
37	206527.0
38	66385.0
39	132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.245716998395906	20.751817524032663	14.189933654151687	23.81253182341974
2	15.234117659911096	20.89135749342278	40.45729664054778	23.417228206118345
3	18.061089624371995	26.645159525453888	29.493500453988297	25.800250396185824
4	12.751781535144232	17.900906451091835	38.69561011709109	30.65170189667284
5	13.238854285050003	38.178697258953235	35.31489764363303	13.267550812363732
6	30.956494171718603	37.97138376414892	18.172373055522968	12.89974900860951
7	26.5032165739347	32.45336918875319	23.26450839890666	17.778905838405443
8	24.4302518759513	36.372391940498574	21.376295350643705	17.82106083290643
9	24.324060116130912	17.80947969190242	22.129933166035503	35.73652702593117
10-11	23.39299784622503	27.741820774171146	28.771646871407587	20.093534508196242
12-13	24.28803507119896	25.272146094783604	28.704039656122827	21.735779177894614
14-15	21.484328077880942	27.042876570042452	26.265472691845908	25.207322660230698
16-17	20.991884656763844	30.419826957539954	27.187799302646397	21.4004890830498
18-19	20.970467232027584	29.43526015259475	28.84771013772674	20.74656247765093
20-21	22.401676695111377	28.714765109678886	27.96012959836242	20.92342859684732
22-23	21.436790207564677	28.663703286941296	28.458444345035	21.44106216045903
24-25	21.660302656656164	28.596659839527653	28.070415615672584	21.6726218881436
26-27	21.665182982303396	28.38492315819336	28.17031392152451	21.77957993797873
28-29	21.435798838903384	28.391768135233164	28.5376470009386	21.634786024924853
30-31	21.886262508765764	28.818593646716977	27.9861182132679	21.30902563124935
32-33	21.27637129168228	28.53145740486669	28.452250618633645	21.73992068481738
34-35	21.37132096632415	28.105828104634593	28.38442681337334	22.13842411566791
36-37	21.835331417664097	28.685608429045722	27.912239579828956	21.566820573461225
38-39	21.617408354125345	28.400930097794458	28.21132343650407	21.770338111576127
40-41	21.762236896702895	28.376285306345	27.971318072155317	21.89015972479679
42-43	21.45667056998999	28.48464781714753	28.32877213595612	21.72990947690636
44-45	21.93016734093417	28.194336002266983	28.142786191885875	21.73271046491297
46-47	21.82841883609937	28.46440175860634	28.20903213454079	21.498147270753503
48-49	21.726843098635232	28.267302706793256	28.26414272033188	21.741711474239626
50-51	21.923222466658693	28.270926576876192	28.05285788825753	21.752993068207584
52-53	21.537703791056057	28.28434215605026	28.11703429875374	22.060919754139938
54-55	21.90822787973569	28.454829428597556	27.82569917040331	21.81124352126344
56-57	21.990290876413695	28.301553825653762	27.800575466344497	21.90757983158804
58-59	21.739892524860434	27.896563969536135	28.20199335354467	22.161550152058755
60-61	22.054187710919507	28.321298233885408	27.815924794746987	21.808589260448098
62-63	21.677134862793906	28.39429691162252	28.08351961902093	21.84504860656265
64-65	21.65698052835015	28.00912913494333	28.18567243581352	22.148217900893002
66-67	21.789898043896212	28.12759657107296	27.89387480525436	22.188630579776472
68-69	22.274665155558587	28.08799336615642	27.79659451817854	21.84074696010645
70-71	22.211391031018994	28.162297044690014	27.70427242276697	21.922039501524022
72-73	21.977211526190167	28.272166648058782	27.974339936380566	21.776281889370487
74-75	22.21659838065008	27.961185530806944	27.693784463488154	22.12843162505483
76-77	22.143665383058313	28.26630244698914	27.74705635488537	21.842975815067174
78-79	22.22541622645407	28.09915445404483	27.850626555188217	21.824802764312885
80-81	22.24320731744975	27.9668191551854	27.815937093979016	21.97403643338583
82-83	21.879372835542725	28.165195412526206	28.035004347627755	21.920427404303314
84-85	22.00263863827569	27.92904074238789	27.74468660682165	22.323634012514766
86-87	22.198063680656944	28.130628835299582	27.716454938551298	21.954852545492177
88-89	22.03574208475752	27.745850808179895	28.044706168493793	22.173700938568796
90-91	22.24780793610902	27.793694674820586	27.7937993912485	22.164697997821897
92-93	21.973261569970504	28.003647644407497	27.957203677872343	22.065887107749653
94-95	21.847108313717474	27.761120828719683	27.928079841640475	22.463691015922368
96-97	22.062210890675907	27.816990071648263	27.92979312134613	22.1910059163297
98-99	22.025367600927073	27.74623730929648	27.915615625167117	22.312779464609335
100	22.18119721721061	27.59777320687694	27.709920180839188	22.511109395073255
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	352.0
1	409.0
2	414.0
3	342.0
4	415.5
5	670.0
6	1076.5
7	1623.0
8	2395.0
9	3445.5
10	4531.5
11	5676.0
12	6748.0
13	7719.5
14	8632.0
15	9376.5
16	10059.0
17	10770.0
18	11670.5
19	12608.0
20	13595.5
21	14753.5
22	16141.5
23	18044.0
24	20500.0
25	23385.0
26	27179.0
27	32362.0
28	38814.5
29	46200.0
30	53997.0
31	61846.5
32	69746.5
33	77392.5
34	84315.0
35	90428.0
36	95614.5
37	100064.0
38	103627.0
39	105195.5
40	105601.0
41	105186.5
42	103249.5
43	100616.5
44	97282.5
45	94193.5
46	91075.0
47	87835.0
48	84919.5
49	82607.5
50	80830.0
51	76497.0
52	69651.0
53	63115.5
54	57955.5
55	53325.5
56	48869.5
57	44598.0
58	39984.0
59	35085.5
60	30448.5
61	25952.0
62	21711.5
63	17950.5
64	14442.5
65	11478.0
66	8943.0
67	6960.5
68	5525.0
69	4234.5
70	3145.0
71	2372.0
72	1851.5
73	1424.5
74	1038.0
75	738.5
76	530.0
77	379.0
78	275.0
79	182.5
80	117.0
81	95.0
82	82.0
83	57.5
84	40.5
85	28.5
86	13.0
87	7.5
88	5.0
89	3.5
90	4.0
91	2.5
92	1.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	9.129050337051915
2	26.756463684078145
3	7.163444695967936
4	25.912798927668035
5	14.411461121878263
6	11.333864211407755
7	9.468833178001052
8	10.563777903713467
9	6.050131449725046
10-11	7.744957962875411
12-13	11.740285122576825
14-15	8.915742413505317
16-17	9.265561841903395
18-19	7.5760754194754325
20-21	9.279373021165894
22-23	6.756345401938366
24-25	6.103741090136905
26-27	11.094447244948048
28-29	7.375239303902649
30-31	4.924346395508341
32-33	10.136466279327449
34-35	8.182445330168939
36-37	8.224592039681571
38-39	7.18708372949153
40-41	9.494385599079905
42-43	7.590912870244841
44-45	7.679259192000789
46-47	6.968905364895182
48-49	4.770579613794898
50-51	7.790687925446072
52-53	5.317599721410772
54-55	6.036981258890729
56-57	5.868098715490751
58-59	4.533458712401499
60-61	5.031182998821005
62-63	3.4113090945397837
64-65	5.127530759444394
66-67	3.345975606743891
68-69	6.192661428159429
70-71	3.196453066516657
72-73	2.003021065005429
74-75	1.0589904341058898
76-77	1.478265830759827
78-79	2.154178681870792
80-81	2.54962370624561
82-83	1.2377356206316057
84-85	1.7498451025801858
86-87	0.49438455541396487
88-89	0.7339058884675957
90-91	0.3340774671480054
92-93	0.5635796071850138
94-95	0.764885372430451
96-97	0.8019876965701311
98-99	0.4672840298585867
100	0.8886119695263461
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2874483.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.21313528741968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.71035071808686	94.01019192620514
2	1.8162334680636252	3.494910327526899
3	0.2600238417781415	0.750531272008648
4	0.08439477899744204	0.324795451569311
5	0.044766789155720535	0.21535765707113658
6	0.02066952367641062	0.11932078066830114
7	0.014255369564563983	0.09600876603412915
8	0.010646630837876496	0.08194765865278605
9	0.00962577246714973	0.08335131737650167
>10	0.027363323593471822	0.4227502183903889
>50	4.284595215932873E-4	0.032265685376462835
>100	0.001059815557569541	0.20525009026376514
>500	1.0890521983704274E-4	0.059306117588405434
>1k	7.260347968119804E-5	0.1040127312681485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.478886464105023E-5	0.0
6	0.0	0.0	0.0	6.957772928210046E-5	0.0
7	0.0	0.0	0.0	1.043665939231507E-4	0.0
8	0.0	0.0	0.0	1.3915545856420093E-4	0.0
9	3.478886464105023E-5	0.0	0.0	1.3915545856420093E-4	0.0
10-11	3.478886464105023E-5	0.0	0.0	1.5654989088472604E-4	0.0
12-13	3.478886464105023E-5	0.0	0.0	2.087331878463014E-4	0.0
14-15	3.478886464105023E-5	0.0	0.0	2.087331878463014E-4	0.0
16-17	3.478886464105023E-5	0.0	0.0	2.087331878463014E-4	0.0
18-19	3.478886464105023E-5	0.0	0.0	2.4352205248735163E-4	0.0
20-21	3.478886464105023E-5	0.0	0.0	2.95705349448927E-4	0.0
22-23	3.478886464105023E-5	3.478886464105023E-5	0.0	3.1309978176945213E-4	0.0
24-25	3.478886464105023E-5	3.478886464105023E-5	0.0	3.6528307873102744E-4	0.0
26-27	3.478886464105023E-5	3.478886464105023E-5	0.0	4.8704410497470325E-4	0.0
28-29	3.478886464105023E-5	6.957772928210046E-5	0.0	7.4796058978258E-4	0.0
30-31	3.478886464105023E-5	6.957772928210046E-5	0.0	0.001791626529014087	0.0
32-33	3.478886464105023E-5	6.957772928210046E-5	0.0	0.004505157971016005	0.0
34-35	3.478886464105023E-5	6.957772928210046E-5	0.0	0.010140954042866144	0.0
36-37	3.478886464105023E-5	6.957772928210046E-5	0.0	0.018264153936551373	0.0
38-39	3.478886464105023E-5	6.957772928210046E-5	0.0	0.03002279018522635	1.7394432320525116E-5
40-41	3.478886464105023E-5	6.957772928210046E-5	0.0	0.04451235230822377	3.478886464105023E-5
42-43	3.478886464105023E-5	6.957772928210046E-5	0.0	0.05928022534834959	3.478886464105023E-5
44-45	3.478886464105023E-5	6.957772928210046E-5	0.0	0.0776313514465036	3.478886464105023E-5
46-47	3.478886464105023E-5	6.957772928210046E-5	0.0	0.09970488606124997	3.478886464105023E-5
48-49	6.957772928210046E-5	6.957772928210046E-5	0.0	0.12343089174644624	3.478886464105023E-5
50-51	6.957772928210046E-5	6.957772928210046E-5	0.0	0.14988782330596495	3.478886464105023E-5
52-53	6.957772928210046E-5	1.043665939231507E-4	0.0	0.1793539916569345	3.478886464105023E-5
54-55	6.957772928210046E-5	1.043665939231507E-4	0.0	0.2118467912316754	3.478886464105023E-5
56-57	6.957772928210046E-5	1.2176102624367581E-4	0.0	0.2522192686476142	3.478886464105023E-5
58-59	6.957772928210046E-5	1.3915545856420093E-4	0.0	0.2956009828550038	3.478886464105023E-5
60-61	6.957772928210046E-5	1.3915545856420093E-4	0.0	0.34230503363561376	3.478886464105023E-5
62-63	6.957772928210046E-5	1.3915545856420093E-4	0.0	0.393392481360996	3.478886464105023E-5
64-65	6.957772928210046E-5	1.3915545856420093E-4	0.0	0.4503070639137542	3.478886464105023E-5
66-67	6.957772928210046E-5	1.3915545856420093E-4	0.0	0.5188585216889438	3.478886464105023E-5
68-69	6.957772928210046E-5	1.3915545856420093E-4	0.0	0.5968029729172168	3.478886464105023E-5
70-71	6.957772928210046E-5	1.7394432320525118E-4	0.0	0.6885412089756662	3.478886464105023E-5
72-73	6.957772928210046E-5	1.7394432320525118E-4	0.0	0.7848541807344138	3.478886464105023E-5
74-75	6.957772928210046E-5	1.7394432320525118E-4	0.0	0.888142319853692	3.478886464105023E-5
76-77	6.957772928210046E-5	1.7394432320525118E-4	0.0	1.0030151508984397	3.478886464105023E-5
78-79	6.957772928210046E-5	1.7394432320525118E-4	0.0	1.1345170592416096	3.478886464105023E-5
80-81	8.697216160262559E-5	1.7394432320525118E-4	0.0	1.285413759622165	3.478886464105023E-5
82-83	1.043665939231507E-4	1.7394432320525118E-4	0.0	1.4489736067320629	3.478886464105023E-5
84-85	1.043665939231507E-4	1.913387555257763E-4	0.0	1.6206044704386842	3.478886464105023E-5
86-87	1.043665939231507E-4	2.087331878463014E-4	0.0	1.805402919411943	3.478886464105023E-5
88	1.043665939231507E-4	2.087331878463014E-4	0.0	1.9642140864983373	3.478886464105023E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8695	0.0	17.197247	1
GTATAAT	1400	0.0	16.15571	1
TACATGG	8805	0.0	16.051405	2
TAAGTCG	215	0.0026198882	15.833051	4
GTACTAG	565	1.0968506E-9	15.567941	1
GTATATA	2185	0.0	15.527227	1
TATACCG	230	5.0268066E-4	15.367258	5
GTATCAA	6270	0.0	14.830156	1
GTACAGG	1230	0.0	14.813048	1
GTATAAA	1895	0.0	14.5879755	1
ACATGGG	9015	0.0	14.537432	3
GTGTATA	1430	0.0	14.498714	1
GTGTTAG	1175	0.0	14.4370165	1
GTATTAA	1570	0.0	14.006187	1
TGTAGGA	1360	0.0	13.856139	2
GTATAGC	635	5.695256E-9	13.851789	1
TATACTG	1350	0.0	13.838663	5
GTATAAG	1100	0.0	13.707874	1
GTACTAC	655	8.80209E-9	13.428832	1
GTGTAGG	900	1.8189894E-12	13.263637	1
>>END_MODULE