FastQCFastQC Report
Wed 25 May 2016
SRR1295158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874483
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47750.16611682866101488No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34870.12130877100334217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89900.019.4768031
GTATACG2553.2729404E-617.7073781
ATCAACG100600.017.103353
TCAACGC101250.017.0405754
CAACGCA102400.016.7573815
TATCAAC103850.016.7034682
AACGCAG103650.016.5804186
GAGTACT39600.016.47644212-13
GAACCGT2355.6716337E-515.9847486
CCTATAC10200.015.6702833
TAGGACC7650.015.3635594
ACGCAGA113650.015.075747
AGTACTT45000.014.91651612-13
CGCAGAG114300.014.8668028
GTACATG113050.014.7783381
CGTACTG2551.1269013E-414.7490165
ACATGGG115500.014.4085213
CATACCG2952.4171746E-514.3427935
TACATGG116700.014.1339022
ATACTCG2000.00570037314.086566
Produced by FastQC (version 0.11.5)