FastQCFastQC Report
Wed 25 May 2016
SRR1295157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944955
Sequences flagged as poor quality0
Sequence length100
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC27450.027.8263174
GTATCAA29900.026.9822651
ATCAACG28450.026.8470233
CAACGCA28700.026.2890325
AACGCAG29500.024.7621576
TATCAAC32900.023.381322
ACGCAGA31450.022.1836557
GTACATG55700.021.8377131
CGCAGAG31650.021.8312538
TACATGG57450.020.9485972
GGTATCA21550.020.1584451
ACATGGG61850.018.7962023
GTATAGA11000.018.6177651
GTACTAC7150.017.3592221
GTATTAC10450.017.2221741
GCAGAGT42900.015.8773389
TCGTACT2504.2179332E-415.7259614
GTCTCGC4855.164111E-815.3548571
GTATAAG10950.015.3022721
GTACTAG5309.293217E-915.2220721
Produced by FastQC (version 0.11.5)