##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295157_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944955 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.73649885991467 33.0 27.0 33.0 15.0 33.0 2 22.757458772714692 33.0 6.0 33.0 2.0 33.0 3 25.150677344815115 33.0 15.0 33.0 15.0 33.0 4 25.198984025222796 33.0 6.0 37.0 2.0 37.0 5 27.03318522693895 33.0 15.0 37.0 2.0 37.0 6 27.84973488559248 33.0 15.0 37.0 6.0 37.0 7 27.836320079593747 33.0 15.0 37.0 6.0 37.0 8 28.744689137864587 33.0 27.0 37.0 6.0 37.0 9 29.697982821469257 37.0 27.0 37.0 15.0 37.0 10-11 29.163007753938516 35.0 24.5 37.0 10.5 37.0 12-13 27.9904509576547 33.0 18.5 37.0 4.0 37.0 14-15 29.83884541529497 35.0 27.0 37.0 10.5 40.0 16-17 30.239067829559367 37.0 27.0 40.0 10.5 40.0 18-19 30.376923076923077 37.0 27.0 40.0 10.5 40.0 20-21 29.88312351122513 35.0 27.0 40.0 6.0 40.0 22-23 30.63539341008606 37.0 27.0 40.0 15.0 40.0 24-25 30.7407271078845 37.0 27.0 40.0 15.0 40.0 26-27 29.318034910550416 33.0 24.5 40.0 4.0 40.0 28-29 30.020693864592157 35.0 27.0 40.0 10.5 40.0 30-31 30.720413384924388 37.0 27.0 40.0 15.0 40.0 32-33 29.522409001156213 33.0 27.0 40.0 6.0 40.0 34-35 29.15988784208927 33.0 22.0 37.0 6.0 40.0 36-37 29.47578910373843 33.0 27.0 37.0 6.0 40.0 38-39 29.586265494718933 33.0 24.5 37.0 6.0 40.0 40-41 28.64140216064422 33.0 22.0 37.0 6.0 40.0 42-43 29.271599735819393 33.0 22.0 37.0 6.0 40.0 44-45 29.13158248598026 33.0 22.0 37.0 6.0 40.0 46-47 28.810705766302032 33.0 22.0 37.0 6.0 40.0 48-49 28.74283427081229 33.0 22.0 37.0 6.0 40.0 50-51 28.684217925231454 33.0 22.0 37.0 6.0 40.0 52-53 28.880435524481697 33.0 22.0 37.0 6.0 40.0 54-55 28.841690280496646 33.0 22.0 37.0 6.0 40.0 56-57 28.85418928302809 33.0 22.0 37.0 6.0 40.0 58-59 28.688757043825795 33.0 22.0 37.0 6.0 40.0 60-61 28.654576895062913 33.0 22.0 37.0 10.5 40.0 62-63 28.61709941238491 33.0 22.0 37.0 10.5 40.0 64-65 28.170411941778397 33.0 22.0 37.0 6.0 40.0 66-67 28.37198887589114 33.0 22.0 37.0 10.5 40.0 68-69 27.953400985753603 33.0 22.0 37.0 6.0 38.5 70-71 28.095686861089558 33.0 22.0 37.0 10.5 37.0 72-73 28.143653977734804 33.0 22.0 37.0 10.5 37.0 74-75 28.255815284104514 33.0 22.0 37.0 15.0 37.0 76-77 28.01271988875891 33.0 22.0 37.0 15.0 37.0 78-79 27.548533169437224 33.0 22.0 37.0 10.5 37.0 80-81 27.36947882055923 33.0 22.0 35.0 10.5 37.0 82-83 27.464154800328018 30.0 22.0 33.0 15.0 37.0 84-85 27.41870062530667 30.0 22.0 33.0 15.0 37.0 86-87 27.231325945557742 27.0 22.0 33.0 15.0 37.0 88-89 27.174154953131712 27.0 22.0 33.0 15.0 37.0 90-91 27.3220665511018 27.0 22.0 33.0 15.0 37.0 92-93 27.25082284788732 27.0 22.0 33.0 15.0 37.0 94-95 27.101610041579583 27.0 22.0 33.0 15.0 37.0 96-97 27.19757992906513 27.0 22.0 33.0 15.0 37.0 98-99 27.271688361961388 27.0 22.0 33.0 15.0 37.0 100 26.95098872478527 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.401173936709988 1101 2 11.427592624415805 1101 3 8.591251869797333 1101 4 12.95698449795374 1101 5 10.902869193820425 1101 6 10.039696045877022 1101 7 9.555970864817766 1101 8 9.186056759972896 1101 9 7.536567157383882 1101 10-11 7.94766131435485 1101 12-13 9.317133045899329 1101 14-15 8.232456188931696 1101 16-17 8.347651927099367 1101 18-19 8.317811209279913 1101 20-21 7.303114071640941 1101 22-23 7.518679729195927 1101 24-25 7.495209576731842 1101 26-27 8.826154363437613 1101 28-29 8.442867957790373 1101 30-31 5.599818681778402 1101 32-33 7.2646663076527815 1101 34-35 8.916822350392586 1101 36-37 8.442908105648232 1101 38-39 8.432116028454772 1101 40-41 9.31301206032219 1101 42-43 8.829765595952143 1101 44-45 8.763558253317374 1101 46-47 7.511678483925312 1101 48-49 5.711739815848247 1101 50-51 7.110818352692451 1101 52-53 6.087723051651967 1101 54-55 4.278354895336776 1101 56-57 6.3864359509243585 1101 58-59 6.462301665938956 1101 60-61 7.008916538142291 1101 62-63 6.537712928604233 1101 64-65 6.959656296419613 1101 66-67 6.113756636733722 1101 68-69 6.992368147602978 1101 70-71 6.506426328529283 1101 72-73 5.772419762894714 1101 74-75 5.153502519672902 1101 76-77 5.308538094734285 1101 78-79 5.356943533605513 1101 80-81 5.147504164811377 1101 82-83 4.766015318323571 1101 84-85 4.450884237867079 1101 86-87 4.43734386408839 1101 88-89 4.2926769762658985 1101 90-91 4.046184772158256 1101 92-93 4.02199944980233 1101 94-95 4.0213009886531985 1101 96-97 4.181734761850215 1101 98-99 3.815024667381561 1101 100 4.016762914924136 1102 1 6.854201167638244 1102 2 10.712980138881044 1102 3 8.300454504785229 1102 4 12.766097820369865 1102 5 10.656599315602687 1102 6 9.728856276758897 1102 7 6.835763605571991 1102 8 7.958384325557471 1102 9 6.578545894419538 1102 10-11 7.585076185227624 1102 12-13 9.18469109738702 1102 14-15 6.391787497917303 1102 16-17 7.529864440501303 1102 18-19 7.593422502184158 1102 20-21 6.9876223073734 1102 22-23 6.66831512961436 1102 24-25 7.0922909541825625 1102 26-27 8.637307421792986 1102 28-29 7.859576021874101 1102 30-31 4.394786633080891 1102 32-33 4.58538795496478 1102 34-35 5.282034882366226 1102 36-37 5.315780322514922 1102 38-39 4.691893200251876 1102 40-41 6.475375332171321 1102 42-43 7.214637394426266 1102 44-45 6.759109921431605 1102 46-47 5.705343976661467 1102 48-49 3.8205975295618764 1102 50-51 5.19341648393646 1102 52-53 6.005787655967875 1102 54-55 5.493810456811325 1102 56-57 6.4755337452795985 1102 58-59 6.262634265417372 1102 60-61 6.1679271003414335 1102 62-63 4.445956469837359 1102 64-65 6.312532249759027 1102 66-67 3.976376756200775 1102 68-69 6.272037369691102 1102 70-71 5.393536762997442 1102 72-73 5.0810623816434415 1102 74-75 4.366213053504481 1102 76-77 3.506279077979915 1102 78-79 4.547693413274349 1102 80-81 4.56735147221271 1102 82-83 4.100425477661087 1102 84-85 3.0403495210027955 1102 86-87 3.3772989975477294 1102 88-89 3.5673285427265142 1102 90-91 3.539832131021491 1102 92-93 3.5313161179348462 1102 94-95 3.3078426190024004 1102 96-97 3.5471113599067863 1102 98-99 3.46671271011072 1102 100 3.521183268972006 1103 1 6.736327021962101 1103 2 11.068560418671101 1103 3 7.9791944032273285 1103 4 12.046798925564588 1103 5 10.357302686544283 1103 6 9.077091183260976 1103 7 8.217639609358457 1103 8 8.251694064781379 1103 9 7.2089399983327205 1103 10-11 7.482067293160544 1103 12-13 8.8204260935543 1103 14-15 8.574288189885305 1103 16-17 8.07882140540968 1103 18-19 7.369084509640839 1103 20-21 8.396886800423665 1103 22-23 6.8837647282481775 1103 24-25 5.293852860150611 1103 26-27 5.999275141331118 1103 28-29 7.048957732696035 1103 30-31 6.094537079366241 1103 32-33 8.024737955224502 1103 34-35 7.334676963370633 1103 36-37 4.10776034535543 1103 38-39 6.3596137030596935 1103 40-41 6.851234838055458 1103 42-43 7.5723914555178204 1103 44-45 5.757468405132446 1103 46-47 6.504588105198575 1103 48-49 5.558044611633612 1103 50-51 6.4842598983180615 1103 52-53 6.092111746323685 1103 54-55 4.987474132411776 1103 56-57 5.972373857013572 1103 58-59 5.50770698327722 1103 60-61 5.096262627277223 1103 62-63 2.7256073419891287 1103 64-65 4.934546182123508 1103 66-67 4.071676976319569 1103 68-69 5.252458237120919 1103 70-71 3.7178818792864057 1103 72-73 4.472613928498021 1103 74-75 3.1886610520164105 1103 76-77 3.595878223823025 1103 78-79 3.0537347082426827 1103 80-81 4.156466388866125 1103 82-83 3.5633353249284774 1103 84-85 3.3822245056978772 1103 86-87 2.8270076784007117 1103 88-89 3.0270781430670723 1103 90-91 2.7494837798594176 1103 92-93 2.2353403292706524 1103 94-95 2.7784623749794832 1103 96-97 3.04307826862415 1103 98-99 2.8372567144522485 1103 100 2.963078140790344 1104 1 6.484716790693369 1104 2 9.880347080284384 1104 3 7.607322088792735 1104 4 12.34759187894661 1104 5 9.687255550493635 1104 6 8.32807855438567 1104 7 4.174564655753315 1104 8 6.786311375372453 1104 9 6.81362222351294 1104 10-11 7.269595317282299 1104 12-13 7.63908347259019 1104 14-15 7.411746908271727 1104 16-17 7.387410018688772 1104 18-19 7.0089020682145 1104 20-21 7.75300985134313 1104 22-23 6.624892702651163 1104 24-25 6.237350372428754 1104 26-27 8.426550475875832 1104 28-29 7.288365849999753 1104 30-31 6.476814627313537 1104 32-33 7.752144463799716 1104 34-35 8.174693346115568 1104 36-37 6.8668874343973805 1104 38-39 7.552616090681759 1104 40-41 7.427538083886798 1104 42-43 7.521776185638078 1104 44-45 6.858942368233574 1104 46-47 6.83572624749922 1104 48-49 5.641260244929093 1104 50-51 5.81468149929329 1104 52-53 6.133458055443054 1104 54-55 5.060517206906912 1104 56-57 6.345476125381886 1104 58-59 4.04937310232415 1104 60-61 4.638241908402787 1104 62-63 4.217190477542591 1104 64-65 3.042743743967609 1104 66-67 3.501610151429002 1104 68-69 4.2871041258211555 1104 70-71 2.7631311379621017 1104 72-73 2.7659520452261006 1104 74-75 3.4630214703673268 1104 76-77 4.103349107008857 1104 78-79 4.647510997173988 1104 80-81 3.263504958106399 1104 82-83 3.2253700710188546 1104 84-85 3.272031188288814 1104 86-87 0.8531817623857094 1104 88-89 0.5892207575106809 1104 90-91 2.4897758827752483 1104 92-93 2.810221139197701 1104 94-95 2.759750004733526 1104 96-97 3.0845565923066403 1104 98-99 2.7640489719751393 1104 100 1.031643290473923 1105 1 5.989852883380085 1105 2 -2.7525442907289452 1105 3 0.48081899646640736 1105 4 8.690049332262994 1105 5 8.825899268227278 1105 6 8.276892497398173 1105 7 5.537765592098346 1105 8 6.194565903148206 1105 9 5.0917655983535255 1105 10-11 5.043177798606774 1105 12-13 5.221830297013025 1105 14-15 5.910896547693891 1105 16-17 6.208040054375818 1105 18-19 6.352864781979999 1105 20-21 7.277964259905097 1105 22-23 2.066543294779443 1105 24-25 4.218591875542881 1105 26-27 7.170747717131054 1105 28-29 6.1776857431019145 1105 30-31 5.4617828672309585 1105 32-33 7.27127067938121 1105 34-35 6.405318164107371 1105 36-37 6.403394032235912 1105 38-39 4.292894735286012 1105 40-41 5.640031765900936 1105 42-43 5.911586480219782 1105 44-45 6.377307636113645 1105 46-47 5.651337218413509 1105 48-49 5.179997524134318 1105 50-51 6.222787377528068 1105 52-53 5.867286040283741 1105 54-55 5.555327354918443 1105 56-57 5.388036984208032 1105 58-59 2.037156148851608 1105 60-61 4.613482960547383 1105 62-63 -0.7704762510994208 1105 64-65 -0.4985481163973695 1105 66-67 0.7950783252630522 1105 68-69 2.9959680406404523 1105 70-71 3.026135944145114 1105 72-73 2.7590855116187747 1105 74-75 2.776142092344191 1105 76-77 1.9672680596883971 1105 78-79 3.3549959859032974 1105 80-81 1.7604068501193808 1105 82-83 0.9464805576429818 1105 84-85 1.523524885416414 1105 86-87 -0.23902251504920713 1105 88-89 1.6048804363810163 1105 90-91 1.4878355523543512 1105 92-93 1.925819410744861 1105 94-95 1.1525587243705502 1105 96-97 2.096113016396121 1105 98-99 1.98519906804707 1105 100 2.157688667990506 1106 1 6.290294643533684 1106 2 10.460144722698189 1106 3 6.92428038598651 1106 4 11.95315380943056 1106 5 9.946861789423554 1106 6 9.458950596703225 1106 7 6.6410702432433695 1106 8 7.5047728387806565 1106 9 6.926322278044015 1106 10-11 5.80791671782713 1106 12-13 8.061732396005457 1106 14-15 7.985682983602427 1106 16-17 7.851507873540331 1106 18-19 7.387832376180409 1106 20-21 8.366587506051872 1106 22-23 6.348457101698937 1106 24-25 5.257714303024034 1106 26-27 7.874437456476304 1106 28-29 6.4661269122962715 1106 30-31 5.626044293536644 1106 32-33 7.555327154282871 1106 34-35 6.937757385114573 1106 36-37 6.148043250816205 1106 38-39 7.322497861199899 1106 40-41 6.84322583989405 1106 42-43 6.361983218171762 1106 44-45 4.62314910566494 1106 46-47 6.224160286743636 1106 48-49 6.059139943745151 1106 50-51 6.578115089599084 1106 52-53 5.757560926435456 1106 54-55 5.371276335175857 1106 56-57 5.041648534572079 1106 58-59 5.019195747626124 1106 60-61 5.156806717481164 1106 62-63 5.1662507592246385 1106 64-65 5.407973717264738 1106 66-67 3.264691399741743 1106 68-69 3.7259714057978357 1106 70-71 3.111674062779251 1106 72-73 2.9023881579683533 1106 74-75 3.0118573126689547 1106 76-77 3.674746808562844 1106 78-79 4.284252537823583 1106 80-81 3.7899006035215095 1106 82-83 2.170384966802242 1106 84-85 2.7649298023853675 1106 86-87 3.4246626672304785 1106 88-89 3.492865061646846 1106 90-91 3.3271986728150367 1106 92-93 3.077410250700286 1106 94-95 1.6855965682879699 1106 96-97 1.6599144562447172 1106 98-99 2.07802474545149 1106 100 3.0963203808967457 1107 1 6.04228476181904 1107 2 10.434691662525605 1107 3 6.138002732499469 1107 4 11.364689576554433 1107 5 9.411872480350237 1107 6 7.953136362552939 1107 7 3.566665720887624 1107 8 6.253385157940041 1107 9 5.676565065695339 1107 10-11 4.470839372446488 1107 12-13 6.210126641416434 1107 14-15 6.185278822338926 1107 16-17 6.949667541220077 1107 18-19 6.238769737495264 1107 20-21 5.45716397226186 1107 22-23 5.729669510963088 1107 24-25 4.570296384194297 1107 26-27 7.213641147117858 1107 28-29 6.3843335700486215 1107 30-31 5.0579129314496 1107 32-33 4.878127015904251 1107 34-35 6.683581300479553 1107 36-37 6.535362506360361 1107 38-39 6.378547538548837 1107 40-41 7.60143548350899 1107 42-43 6.070696311395583 1107 44-45 4.222005594819432 1107 46-47 3.4360664859425007 1107 48-49 4.04490942361366 1107 50-51 4.755000402184283 1107 52-53 3.896662083228822 1107 54-55 5.244945029921173 1107 56-57 5.198541670021093 1107 58-59 3.155887118579404 1107 60-61 2.75725166389671 1107 62-63 0.32020298830809324 1107 64-65 1.8698759857990872 1107 66-67 4.023472780748868 1107 68-69 5.0062921672553 1107 70-71 4.334724128626899 1107 72-73 2.405903458230256 1107 74-75 2.5135805544598746 1107 76-77 3.041556950494691 1107 78-79 3.8061587802958208 1107 80-81 2.091435534026882 1107 82-83 1.552334994713565 1107 84-85 2.2202346969401816 1107 86-87 2.329553279361157 1107 88-89 2.6146187294256364 1107 90-91 1.354107341000116 1107 92-93 2.2664301857806137 1107 94-95 2.2483405760079407 1107 96-97 2.1984725562744813 1107 98-99 1.7887976150673772 1107 100 0.021875421980002585 1108 1 4.465083740433073 1108 2 -3.0920275129245915 1108 3 0.08100922477566286 1108 4 6.838649110488657 1108 5 7.235430842796564 1108 6 7.006874893582761 1108 7 4.311144996393118 1108 8 5.305004869507535 1108 9 5.018560564772979 1108 10-11 3.4760350085757814 1108 12-13 2.634069975209691 1108 14-15 4.207319971290172 1108 16-17 4.8347609912972445 1108 18-19 4.094122587954853 1108 20-21 4.865321025005983 1108 22-23 4.225860277084426 1108 24-25 3.0876962491120565 1108 26-27 4.802855910684183 1108 28-29 6.169889676220468 1108 30-31 4.6919881928793075 1108 32-33 4.708639147976868 1108 34-35 3.1273302942734276 1108 36-37 1.3041564316693517 1108 38-39 2.568380659894242 1108 40-41 5.493766451855066 1108 42-43 3.104490861338352 1108 44-45 3.808616519397244 1108 46-47 4.98629334130354 1108 48-49 4.99241065716156 1108 50-51 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2213 42-43 -23.934106167303096 2213 44-45 -17.76264686434891 2213 46-47 -17.582616440563818 2213 48-49 -6.51759810715491 2213 50-51 -24.076912843361807 2213 52-53 -18.39619808612244 2213 54-55 -18.251449635315474 2213 56-57 -18.147153213028552 2213 58-59 -18.620677957191713 2213 60-61 -20.01192746577816 2213 62-63 -18.196890863274525 2213 64-65 -18.194466238806502 2213 66-67 -9.078116459686228 2213 68-69 -23.413072666207132 2213 70-71 -18.065857809445358 2213 72-73 -9.819249143277887 2213 74-75 -4.5038555746919755 2213 76-77 -3.393973050101149 2213 78-79 -5.010766084150344 2213 80-81 -3.4213012300266925 2213 82-83 -3.491422080898662 2213 84-85 -3.2268013249602845 2213 86-87 -3.437255396084236 2213 88-89 -3.1106265584648867 2213 90-91 -3.099049954411246 2213 92-93 -3.9716801374962465 2213 94-95 -3.945545101959844 2213 96-97 -3.4944354458705504 2213 98-99 -3.9399836583111068 2213 100 -3.3301275969478823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 750.0 3 760.0 4 4205.0 5 14223.0 6 20627.0 7 25574.0 8 23398.0 9 22607.0 10 26869.0 11 33328.0 12 40468.0 13 53031.0 14 38348.0 15 42090.0 16 48920.0 17 53284.0 18 53903.0 19 54866.0 20 54711.0 21 53520.0 22 56430.0 23 62654.0 24 69775.0 25 78200.0 26 86758.0 27 94926.0 28 103252.0 29 108766.0 30 114725.0 31 125901.0 32 144529.0 33 176043.0 34 215704.0 35 253883.0 36 278610.0 37 236822.0 38 72334.0 39 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.74471576216446 21.05875909680038 14.081123257570708 24.115401883464457 2 14.824100701617004 21.634286074320098 40.46259917822152 23.07901404584138 3 17.32333176855136 26.786133423742957 30.53708243503257 25.353452372673114 4 12.419434050200694 17.814188194902282 39.40474454611571 30.36163320878132 5 13.42031332801209 37.48752227576633 35.572959652224746 13.519204743996834 6 30.1293092744639 38.24214185040789 18.575378111485605 13.053170763642601 7 25.77182254046081 31.849899655868008 24.869685583462164 17.50859222020902 8 23.503562080379798 36.871768079062875 22.098944530240896 17.525725310316435 9 24.17411262045346 17.39008498725442 22.483668267108524 35.9521341251836 10-11 23.106746046932034 27.46068799874624 29.36395560099207 20.068610353329653 12-13 23.61975643525616 25.223976354459943 29.51397121658354 21.64229599370035 14-15 21.20080663900597 26.61938017413456 26.707932481246267 25.471880705613202 16-17 21.107588011579953 30.248123179801045 27.323839953571046 21.32044885504796 18-19 20.872505169781576 29.326760951447273 28.93219601475037 20.868537864020777 20-21 22.451092987665664 28.625216434342793 27.823037193036832 21.10065338495471 22-23 21.542059802866255 28.578827833853833 28.56200711836831 21.3171052449116 24-25 21.7936899518269 28.24736034734869 28.073811595633025 21.885138105191395 26-27 21.618205661316324 28.550509687240883 28.132102403831123 21.69918224761167 28-29 21.37179341076353 28.3928637533925 28.50136208136776 21.733980754476214 30-31 22.02989665734178 28.72575283712344 27.789046977064892 21.455303528469887 32-33 21.249955534597063 28.56688425380908 28.461526857196496 21.721633354397362 34-35 21.502461306131167 27.96770669776712 28.154682554431886 22.375149441669826 36-37 21.92331066341963 28.669348778640767 27.829677986059536 21.577662571880065 38-39 21.478819767349368 28.42847287405828 28.1861826985796 21.906524660012757 40-41 21.949937738677683 28.331166376955363 27.660864025281732 22.058031859085215 42-43 21.441369916297866 28.531944841176955 28.345919918122885 21.680765324402294 44-45 21.85028142077744 28.01335556231914 28.18634189330995 21.950021123593462 46-47 21.85617546435022 28.487111228069352 28.157840848070947 21.498872459509485 48-49 21.567433221298426 28.148876702635768 28.3226074650628 21.961082611003008 50-51 22.03239966182416 28.18725813712863 27.942979594079816 21.837362606967396 52-53 21.461209961753582 28.30613930008538 28.336971994555082 21.895678743605952 54-55 21.893544149653927 28.062472186011373 28.09788806863956 21.946095595695144 56-57 21.8906614739491 28.315686262968192 27.97933329801601 21.814318965066704 58-59 21.558086750216575 27.796624595061235 28.398457268520527 22.246831386201656 60-61 22.006309490777046 28.2157574759766 27.816597558664924 21.961335474581432 62-63 21.576287018448816 28.353993767251236 28.313713502144687 21.756005712155265 64-65 21.744570819780083 27.721321105713848 28.210066186344235 22.324041888161837 66-67 21.694016908055907 28.199776009751805 28.013216261468866 22.092990820723422 68-69 21.968912306789782 28.006089179511534 28.053865708557442 21.97113280514124 70-71 22.234126076576374 28.06827753358026 27.696356653576686 22.00123973626668 72-73 21.73591951235761 28.25916167565457 28.29994466767988 21.704974144307933 74-75 21.966705356161846 27.7844199878763 27.90074518637532 22.34812946958653 76-77 22.107682041556608 28.23860586405581 27.793184760035157 21.860527334352426 78-79 21.938161189581567 27.903391012791822 28.087138941543472 22.07130885608314 80-81 22.248333450697995 27.902182393011127 27.774491952657755 22.074992203633123 82-83 21.72841796935178 28.075691419909088 28.40110879152405 21.794781819215082 84-85 21.949242769545663 27.633347380008427 27.9365491619295 22.48086068851641 86-87 22.072962827389656 28.080784119654727 27.992850818086453 21.85340223486917 88-89 21.812501164179864 27.66453642400516 28.247130478203218 22.275831933611755 90-91 22.165736585299438 27.703064928699433 27.820495147068584 22.31070333893254 92-93 21.799148526785093 27.941651907093558 28.237371409454916 22.02182815666644 94-95 21.866107167611165 27.418368014294614 28.079531558994574 22.635993259099642 96-97 21.94157280342946 27.812865596953106 28.05332058102696 22.192241018590472 98-99 21.734494990107812 27.661789887144796 28.171837135601947 22.431877987145445 100 22.226942631008107 27.30824295890983 27.74327446877501 22.72153994130706 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 316.0 1 373.5 2 356.5 3 289.5 4 351.0 5 496.5 6 738.5 7 1069.5 8 1565.5 9 2305.0 10 3198.5 11 4138.5 12 5087.5 13 6109.0 14 7030.5 15 7828.5 16 8625.0 17 9452.5 18 10375.5 19 11323.0 20 12307.5 21 13399.0 22 14618.5 23 16132.5 24 18033.0 25 20755.0 26 24806.0 27 30157.0 28 36816.0 29 44868.0 30 53801.0 31 62877.5 32 72142.0 33 80876.0 34 88458.5 35 95397.0 36 101748.5 37 107316.0 38 111372.5 39 114042.5 40 115956.5 41 115868.5 42 113208.0 43 109542.5 44 105174.5 45 100000.5 46 94377.0 47 88627.0 48 83664.0 49 79104.5 50 75305.0 51 71208.0 52 66508.5 53 62499.0 54 58682.5 55 54780.0 56 50710.0 57 46515.0 58 41800.0 59 36985.5 60 32546.5 61 28157.5 62 23701.0 63 19479.5 64 15848.0 65 12737.0 66 9912.0 67 7575.0 68 5799.0 69 4457.0 70 3442.0 71 2627.0 72 2042.5 73 1552.0 74 1140.5 75 834.0 76 577.0 77 389.5 78 267.0 79 164.5 80 108.0 81 87.5 82 68.5 83 43.5 84 23.0 85 16.5 86 11.5 87 9.0 88 6.5 89 4.5 90 4.0 91 3.5 92 1.5 93 0.5 94 0.5 95 1.0 96 0.5 97 0.5 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.804940992307182 2 24.54859242331377 3 6.254323071150493 4 24.082167639233877 5 12.40623371155077 6 9.75026783091762 7 8.056931260409751 8 8.61918093824863 9 5.075697251740689 10-11 6.832022900180139 12-13 9.918929151718787 14-15 7.2790755716131486 16-17 7.906895011978111 18-19 6.706333373515045 20-21 7.974230506068854 22-23 5.725605314852009 24-25 5.369521775375175 26-27 9.863783996699441 28-29 6.438570368647399 30-31 4.314089688976572 32-33 8.838284455959428 34-35 7.1799738875466685 36-37 6.912940944768256 38-39 6.087121874527794 40-41 8.538975977561627 42-43 6.5047004113814975 44-45 6.563512855035136 46-47 6.062418610810692 48-49 4.022302547916691 50-51 6.698642254295906 52-53 4.571428086337482 54-55 5.079839929642389 56-57 4.814963216755435 58-59 3.8490910726989034 60-61 4.30094857137036 62-63 3.1389783545079637 64-65 4.653704385975337 66-67 2.613724148586311 68-69 5.188160769858962 70-71 2.601160289376238 72-73 1.6269858113281868 74-75 0.7947150296014709 76-77 1.1278949933021047 78-79 1.7887370095638133 80-81 2.112154515094458 82-83 0.8128137781392245 84-85 1.2896631697258532 86-87 0.37056593394466125 88-89 0.6477348550317407 90-91 0.23905288875381794 92-93 0.3250304334022082 94-95 0.5724875252762776 96-97 0.5901618191109881 98-99 0.4704316364766185 100 0.4096497230008608 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2944955.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.70566343175982 #Duplication Level Percentage of deduplicated Percentage of total 1 96.54001851231212 91.42886500922891 2 2.677128847110804 5.070785271158619 3 0.4365039603517529 1.2401819146711002 4 0.13524960886265533 0.5123561574489518 5 0.06436408180219064 0.30478215341262616 6 0.04138783045792964 0.2351797164911653 7 0.02776230339004909 0.18404731526638754 8 0.018487844104993687 0.14007228331091012 9 0.012296940568281617 0.10481309232300288 >10 0.04585648117263538 0.6837902814130242 >50 7.27605373367908E-4 0.04574982624868058 >100 2.1598449321922546E-4 0.04937697902653818 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.6978188121720027E-5 0.0 0.0 0.0 0.0 14-15 5.0934564365160085E-5 0.0 0.0 5.0934564365160085E-5 0.0 16-17 1.3582550497376022E-4 0.0 0.0 6.791275248688011E-5 0.0 18-19 1.6978188121720026E-4 0.0 0.0 1.0186912873032016E-4 0.0 20-21 1.6978188121720026E-4 0.0 0.0 1.6978188121720026E-4 0.0 22-23 1.6978188121720026E-4 0.0 0.0 1.6978188121720026E-4 0.0 24-25 1.6978188121720026E-4 0.0 0.0 2.037382574606403E-4 0.0 26-27 1.6978188121720026E-4 0.0 0.0 3.2258557431268054E-4 0.0 28-29 2.037382574606403E-4 0.0 0.0 5.093456436516008E-4 0.0 30-31 2.037382574606403E-4 0.0 0.0 0.0016808406240502827 0.0 32-33 2.037382574606403E-4 0.0 0.0 0.004940652743420528 0.0 34-35 2.037382574606403E-4 0.0 0.0 0.010662302140440175 0.0 36-37 2.037382574606403E-4 0.0 0.0 0.01964376365683007 0.0 38-39 2.037382574606403E-4 0.0 0.0 0.03332818328293641 0.0 40-41 2.037382574606403E-4 0.0 0.0 0.04955933112730075 0.0 42-43 2.037382574606403E-4 0.0 0.0 0.06691103938769863 0.0 44-45 2.037382574606403E-4 0.0 0.0 0.08966181147080346 0.0 46-47 2.037382574606403E-4 0.0 0.0 0.11709856347550301 0.0 48-49 2.3769463370408036E-4 0.0 0.0 0.14613126516364427 0.0 50-51 2.3769463370408036E-4 3.3956376243440054E-5 0.0 0.17759184775319148 0.0 52-53 2.3769463370408036E-4 3.3956376243440054E-5 0.0 0.2117689404422139 3.3956376243440054E-5 54-55 2.3769463370408036E-4 5.0934564365160085E-5 0.0 0.25117531507272606 3.3956376243440054E-5 56-57 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.29845956899171633 3.3956376243440054E-5 58-59 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.3513126686146308 3.3956376243440054E-5 60-61 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.4105665451594337 5.0934564365160085E-5 62-63 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.4712296113183393 6.791275248688011E-5 64-65 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.5416042010828688 6.791275248688011E-5 66-67 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.6277005930481111 6.791275248688011E-5 68-69 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.7231519666684211 6.791275248688011E-5 70-71 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.8304201592214482 6.791275248688011E-5 72-73 2.3769463370408036E-4 6.791275248688011E-5 0.0 0.9469075079245692 6.791275248688011E-5 74-75 2.3769463370408036E-4 6.791275248688011E-5 0.0 1.073004511104584 6.791275248688011E-5 76-77 2.3769463370408036E-4 6.791275248688011E-5 0.0 1.2153156839408412 6.791275248688011E-5 78-79 2.3769463370408036E-4 6.791275248688011E-5 0.0 1.3723129895023862 6.791275248688011E-5 80-81 2.3769463370408036E-4 1.0186912873032016E-4 0.0 1.5526043691669313 6.791275248688011E-5 82-83 2.3769463370408036E-4 1.0186912873032016E-4 0.0 1.7431505744569953 6.791275248688011E-5 84-85 2.3769463370408036E-4 1.0186912873032016E-4 0.0 1.947279330244435 8.489094060860013E-5 86-87 2.3769463370408036E-4 1.0186912873032016E-4 0.0 2.1797616601951475 1.0186912873032016E-4 88 2.3769463370408036E-4 1.0186912873032016E-4 0.0 2.370868145693228 1.0186912873032016E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 2745 0.0 27.826317 4 GTATCAA 2990 0.0 26.982265 1 ATCAACG 2845 0.0 26.847023 3 CAACGCA 2870 0.0 26.289032 5 AACGCAG 2950 0.0 24.762157 6 TATCAAC 3290 0.0 23.38132 2 ACGCAGA 3145 0.0 22.183655 7 GTACATG 5570 0.0 21.837713 1 CGCAGAG 3165 0.0 21.831253 8 TACATGG 5745 0.0 20.948597 2 GGTATCA 2155 0.0 20.158445 1 ACATGGG 6185 0.0 18.796202 3 GTATAGA 1100 0.0 18.617765 1 GTACTAC 715 0.0 17.359222 1 GTATTAC 1045 0.0 17.222174 1 GCAGAGT 4290 0.0 15.877338 9 TCGTACT 250 4.2179332E-4 15.725961 4 GTCTCGC 485 5.164111E-8 15.354857 1 GTATAAG 1095 0.0 15.302272 1 GTACTAG 530 9.293217E-9 15.222072 1 >>END_MODULE