FastQCFastQC Report
Wed 25 May 2016
SRR1295157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944955
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50900.034.5848431
TCGACGC753.2656698E-431.3514774
ATCAACG59000.029.1713263
GGTATCA33100.028.9954361
TCAACGC59600.028.8002224
CAACGCA59750.028.5705095
TATCAAC61000.027.9381432
AACGCAG62650.027.1360116
ACGCAGA71000.023.8732437
CGCAGAG72350.023.2979938
CCTATAC7500.020.0639213
GTACATG72800.019.4676651
GCAGAGT86550.019.0951659
TATACTG16600.017.8476775
AGAGTAC78500.017.52450810-11
TACATGG77350.017.3434982
ACATGGG77150.017.127643
GACTGCG3652.6775524E-916.7229587
GAGTACT38550.016.50299512-13
CGTATAC1750.002321571316.1227953
Produced by FastQC (version 0.11.5)