##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295157_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944955 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.498803886646826 33.0 33.0 33.0 27.0 33.0 2 30.195484141523384 33.0 33.0 33.0 27.0 33.0 3 30.99107151043055 33.0 33.0 33.0 27.0 33.0 4 35.27491421770451 37.0 37.0 37.0 33.0 37.0 5 35.5551500786939 37.0 37.0 37.0 33.0 37.0 6 35.340283298047 37.0 37.0 37.0 33.0 37.0 7 35.255817830832726 37.0 37.0 37.0 33.0 37.0 8 35.24774809801848 37.0 37.0 37.0 33.0 37.0 9 35.246827540658515 37.0 37.0 37.0 33.0 37.0 10-11 35.20764222203735 37.0 37.0 37.0 33.0 37.0 12-13 35.12834202899535 37.0 37.0 37.0 33.0 37.0 14-15 36.60919131192158 40.0 37.0 40.0 33.0 40.0 16-17 36.6003426198363 40.0 37.0 40.0 33.0 40.0 18-19 36.62936734177602 40.0 37.0 40.0 33.0 40.0 20-21 36.629256134643825 40.0 37.0 40.0 33.0 40.0 22-23 36.58757281520431 40.0 37.0 40.0 33.0 40.0 24-25 36.560017385664636 40.0 37.0 40.0 33.0 40.0 26-27 36.53540818110973 40.0 37.0 40.0 33.0 40.0 28-29 36.4841596900462 40.0 37.0 40.0 33.0 40.0 30-31 36.40363723724131 37.0 37.0 40.0 33.0 40.0 32-33 36.36557451641876 38.5 37.0 40.0 33.0 40.0 34-35 36.26720459226033 37.0 37.0 40.0 33.0 40.0 36-37 36.25417909611522 37.0 37.0 40.0 33.0 40.0 38-39 36.19623389831084 37.0 37.0 40.0 27.0 40.0 40-41 36.138697535276435 37.0 37.0 40.0 27.0 40.0 42-43 36.04952944951621 37.0 37.0 40.0 27.0 40.0 44-45 35.9506296361065 37.0 37.0 40.0 27.0 40.0 46-47 35.84833656201877 37.0 37.0 40.0 27.0 40.0 48-49 35.73959415339114 37.0 37.0 40.0 27.0 40.0 50-51 35.63453040878383 37.0 37.0 40.0 27.0 40.0 52-53 35.5337723326842 37.0 37.0 40.0 27.0 40.0 54-55 35.412473365467385 37.0 35.0 40.0 27.0 40.0 56-57 35.28903871196674 37.0 33.0 40.0 27.0 40.0 58-59 35.15844333784388 37.0 33.0 40.0 27.0 40.0 60-61 35.004384786864314 37.0 33.0 40.0 27.0 40.0 62-63 34.82735763364805 37.0 33.0 40.0 27.0 40.0 64-65 34.640670570518054 37.0 33.0 40.0 27.0 40.0 66-67 34.419392826036386 37.0 33.0 40.0 27.0 40.0 68-69 34.19703526879019 37.0 33.0 37.0 27.0 40.0 70-71 33.97083503822639 37.0 33.0 37.0 27.0 40.0 72-73 33.76382423500529 37.0 33.0 37.0 27.0 40.0 74-75 33.53156177259075 37.0 33.0 37.0 27.0 40.0 76-77 33.29732287929697 37.0 33.0 37.0 27.0 38.5 78-79 33.07403457777793 37.0 33.0 37.0 24.5 37.0 80-81 32.85892534860464 37.0 33.0 37.0 22.0 37.0 82-83 32.64515977323932 37.0 33.0 37.0 22.0 37.0 84-85 32.48093553212188 35.0 33.0 37.0 22.0 37.0 86-87 32.51331463468882 35.0 33.0 37.0 22.0 37.0 88-89 32.52765746845028 37.0 33.0 37.0 22.0 37.0 90-91 32.539881424334155 37.0 33.0 37.0 22.0 37.0 92-93 32.51377372489563 33.0 33.0 37.0 22.0 37.0 94-95 32.54589238205678 35.0 33.0 37.0 22.0 37.0 96-97 32.5092543009995 33.0 33.0 37.0 22.0 37.0 98-99 32.44130979930084 33.0 33.0 37.0 22.0 37.0 100 31.911733116465278 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6557349857993877 1101 2 4.175069465412275 1101 3 2.3806665769140842 1101 4 1.2116730895635257 1101 5 0.6326462638767225 1101 6 0.6531636310311697 1101 7 0.6648909888885512 1101 8 0.6500967566766818 1101 9 0.6868220276608952 1101 10-11 0.7521659865004082 1101 12-13 0.7365001326935641 1101 14-15 1.0781050531677039 1101 16-17 1.156779129162956 1101 18-19 1.1139139308380521 1101 20-21 1.1279227044078297 1101 22-23 1.0779572922531528 1101 24-25 1.0576829284104647 1101 26-27 1.1025330194231273 1101 28-29 1.0634156148735983 1101 30-31 0.9846086146811999 1101 32-33 1.159750550573996 1101 34-35 1.248174669771842 1101 36-37 1.104135295575439 1101 38-39 1.099339024308037 1101 40-41 1.1124530951662166 1101 42-43 1.1628622872202001 1101 44-45 1.1907023189545 1101 46-47 1.2597690311258916 1101 48-49 1.1471523605560634 1101 50-51 1.1299684086738608 1101 52-53 1.131290894722703 1101 54-55 1.1655418882430482 1101 56-57 1.1278941733216996 1101 58-59 1.2074082548473584 1101 60-61 1.1984337976964312 1101 62-63 1.2169735199609804 1101 64-65 1.1770601030632264 1101 66-67 1.0797007472274487 1101 68-69 1.1313467569105953 1101 70-71 1.1242700550182434 1101 72-73 0.9717428327541384 1101 74-75 0.9809382358394814 1101 76-77 1.0343013959614566 1101 78-79 1.0867431004953048 1101 80-81 1.1103003627943622 1101 82-83 1.1068207808894428 1101 84-85 1.0634988701969235 1101 86-87 1.1562651506089168 1101 88-89 1.0349648030236622 1101 90-91 1.074301891465602 1101 92-93 1.1609898129171725 1101 94-95 1.1728143809054075 1101 96-97 1.0531803654817153 1101 98-99 1.0260595176126657 1101 100 1.1975244764027018 1102 1 0.6437038178625016 1102 2 4.139166971088191 1102 3 2.3618380934254795 1102 4 1.1809032414646623 1102 5 0.5674509011059214 1102 6 0.5808770530993996 1102 7 0.5752548557057722 1102 8 0.5620724645039559 1102 9 0.6573664403014305 1102 10-11 0.751908436429467 1102 12-13 0.7909697835474674 1102 14-15 1.0768309669676768 1102 16-17 1.0472591008800762 1102 18-19 1.0117906126116338 1102 20-21 0.9901074112549324 1102 22-23 0.966664556406478 1102 24-25 1.0194072254137652 1102 26-27 0.9671353795305677 1102 28-29 0.9979085506018421 1102 30-31 0.9538890809829965 1102 32-33 1.0773640092467645 1102 34-35 1.2044012735154013 1102 36-37 1.0343883258904256 1102 38-39 1.0430193479444938 1102 40-41 0.9935162871666421 1102 42-43 1.0908273895464333 1102 44-45 1.1072421431333979 1102 46-47 1.070285323032273 1102 48-49 0.9197935762270362 1102 50-51 0.9093657309229286 1102 52-53 0.9749259341957455 1102 54-55 0.9517852286515875 1102 56-57 0.9613570194265506 1102 58-59 0.9105750862857676 1102 60-61 0.9492692837756422 1102 62-63 0.9907774637824431 1102 64-65 0.9483862280417412 1102 66-67 0.9069393423252521 1102 68-69 0.9155792413406729 1102 70-71 0.8574643716151726 1102 72-73 0.9764672984423939 1102 74-75 0.9379135053553114 1102 76-77 0.876187447688558 1102 78-79 0.8616407059387896 1102 80-81 0.8271474428126169 1102 82-83 0.8906431722820258 1102 84-85 0.8779035042121563 1102 86-87 0.8618574055928292 1102 88-89 0.8310093718397056 1102 90-91 0.899651477316624 1102 92-93 0.9897170310665544 1102 94-95 0.9134230651108197 1102 96-97 0.9965764482379882 1102 98-99 0.9730452074898537 1102 100 1.010943835854075 1103 1 0.5150414025219092 1103 2 4.059270799680977 1103 3 2.338223915489653 1103 4 1.0604055538374482 1103 5 0.388534838370461 1103 6 0.4520759226005353 1103 7 0.4988363831407199 1103 8 0.4937606413370901 1103 9 0.5213811112598279 1103 10-11 0.5552911025259064 1103 12-13 0.6142411188564836 1103 14-15 0.8368633899044511 1103 16-17 0.837251672891135 1103 18-19 0.88658394359841 1103 20-21 0.812193409706019 1103 22-23 0.7492416431259628 1103 24-25 0.8180195049208479 1103 26-27 0.8212086531909861 1103 28-29 0.8212118438253455 1103 30-31 0.7702771217971076 1103 32-33 0.930890028356707 1103 34-35 0.9533365827317226 1103 36-37 0.8726417763928822 1103 38-39 0.8152259182641615 1103 40-41 0.8572937225538979 1103 42-43 0.8694960628996924 1103 44-45 0.9094403133696431 1103 46-47 0.8428109654246967 1103 48-49 0.7313300989041664 1103 50-51 0.7300340668876828 1103 52-53 0.8029529757363392 1103 54-55 0.8402881710306289 1103 56-57 0.8407404666614937 1103 58-59 0.9192145201260828 1103 60-61 0.808058588362023 1103 62-63 0.8054243671668928 1103 64-65 0.8050213910976112 1103 66-67 0.8054387426196499 1103 68-69 0.8446194378175349 1103 70-71 0.8017651648492006 1103 72-73 0.7109496531965149 1103 74-75 0.6959450214986305 1103 76-77 0.7024786575058641 1103 78-79 0.7392794909740772 1103 80-81 0.7247060387124549 1103 82-83 0.7666290628133012 1103 84-85 0.7534834278149276 1103 86-87 0.7385514780675351 1103 88-89 0.662034013815834 1103 90-91 0.6878775088189215 1103 92-93 0.7970381019930883 1103 94-95 0.6363461284104943 1103 96-97 0.7194220531713853 1103 98-99 0.7413850680375873 1103 100 0.841851245217395 1104 1 0.5014233986187833 1104 2 3.5976534926558053 1104 3 2.1862257656802484 1104 4 0.94870758115119 1104 5 0.3863498101412972 1104 6 0.3644186602203874 1104 7 0.32947731218906995 1104 8 0.2678341833568396 1104 9 0.3204418033187295 1104 10-11 0.43351089009019717 1104 12-13 0.42670247345886025 1104 14-15 0.5983183213093497 1104 16-17 0.6184834709024827 1104 18-19 0.5757679443401216 1104 20-21 0.5519698585932318 1104 22-23 0.54509901441876 1104 24-25 0.5853687859598082 1104 26-27 0.6700194523541185 1104 28-29 0.540513605734553 1104 30-31 0.5214479582697038 1104 32-33 0.6490453037686805 1104 34-35 0.7333857768137619 1104 36-37 0.6505593542733195 1104 38-39 0.6322211822448907 1104 40-41 0.6350660411147473 1104 42-43 0.7172078019820631 1104 44-45 0.743669476183868 1104 46-47 0.7215109518233405 1104 48-49 0.5385642830093431 1104 50-51 0.5321072533366618 1104 52-53 0.4834852977815771 1104 54-55 0.501992689683064 1104 56-57 0.5580902061727926 1104 58-59 0.5272242611350606 1104 60-61 0.6049957129559616 1104 62-63 0.6322227885956835 1104 64-65 0.6040660366246229 1104 66-67 0.6113383901562983 1104 68-69 0.6511998913078116 1104 70-71 0.5707705498615354 1104 72-73 0.5398240883203869 1104 74-75 0.5650763335802509 1104 76-77 0.610070781154846 1104 78-79 0.6106616984776423 1104 80-81 0.4949623909740737 1104 82-83 0.5923296212835609 1104 84-85 0.620501640883333 1104 86-87 0.6651366334463589 1104 88-89 0.6248331097083835 1104 90-91 0.6775068390264849 1104 92-93 0.6664241464007219 1104 94-95 0.6501731702297917 1104 96-97 0.6377073819036951 1104 98-99 0.6014158315315754 1104 100 0.5564454386142721 1105 1 0.5196178285668118 1105 2 3.764714732310818 1105 3 2.1849645521745735 1105 4 0.9520683489719701 1105 5 0.416692320796038 1105 6 0.3823222320593942 1105 7 0.33853534880402236 1105 8 0.38762593402241663 1105 9 0.41732412897452065 1105 10-11 0.4281181728120842 1105 12-13 0.43678736345363234 1105 14-15 0.5057013651589841 1105 16-17 0.5440040917027247 1105 18-19 0.5584389884340055 1105 20-21 0.5620698874102388 1105 22-23 0.480385164531576 1105 24-25 0.5277276822468551 1105 26-27 0.4929784576406817 1105 28-29 0.46458967352361213 1105 30-31 0.4228704648955386 1105 32-33 0.5419658275384549 1105 34-35 0.653996651874202 1105 36-37 0.5037885233807131 1105 38-39 0.5197397330077109 1105 40-41 0.48998832392944536 1105 42-43 0.5735808251992864 1105 44-45 0.5991150246550276 1105 46-47 0.5668140979901182 1105 48-49 0.39164538581346164 1105 50-51 0.3014790627094186 1105 52-53 0.31237375118630695 1105 54-55 0.42366779605294624 1105 56-57 0.3181856705207835 1105 58-59 0.40812874414351796 1105 60-61 0.384385365345274 1105 62-63 0.4682275281374473 1105 64-65 0.41371218478586513 1105 66-67 0.37626225126296475 1105 68-69 0.3975124850125553 1105 70-71 0.3318702988258764 1105 72-73 0.36424007211713416 1105 74-75 0.39539032137525965 1105 76-77 0.37527644750696254 1105 78-79 0.3450758372145728 1105 80-81 0.39972723003749167 1105 82-83 0.3902552273360058 1105 84-85 0.42195418678692675 1105 86-87 0.4919796468520019 1105 88-89 0.36155385214665614 1105 90-91 0.3477801462449648 1105 92-93 0.30554566260777705 1105 94-95 0.33489081572655266 1105 96-97 0.4088243652887229 1105 98-99 0.42269922024205897 1105 100 0.3376311334872284 1106 1 0.5828493414048488 1106 2 3.9017149804862754 1106 3 2.1911881835319633 1106 4 1.0792496535835454 1106 5 0.4334599323902779 1106 6 0.3657871747782693 1106 7 0.2326176079019504 1106 8 0.2985327557918893 1106 9 0.35312513338977425 1106 10-11 0.32712785087151275 1106 12-13 0.30364735038084234 1106 14-15 0.45345435868978257 1106 16-17 0.4103050962159145 1106 18-19 0.4310777957893279 1106 20-21 0.4937415469675699 1106 22-23 0.29065527031856675 1106 24-25 0.4312504026416448 1106 26-27 0.4233785607500522 1106 28-29 0.37489214299675666 1106 30-31 0.32734186389802034 1106 32-33 0.43985621860382906 1106 34-35 0.5160914789926778 1106 36-37 0.44030650904999646 1106 38-39 0.34781407752187476 1106 40-41 0.45814314722048977 1106 42-43 0.3692774539109749 1106 44-45 0.43424863393909874 1106 46-47 0.33060730123406756 1106 48-49 0.2990148258359966 1106 50-51 0.30774568894719323 1106 52-53 0.25859271382855553 1106 54-55 0.2851685054194988 1106 56-57 0.37363591800088614 1106 58-59 0.2763016174492634 1106 60-61 0.335103242734057 1106 62-63 0.4213928327772294 1106 64-65 0.325911374875929 1106 66-67 0.30509977449145964 1106 68-69 0.2254479041558355 1106 70-71 0.29591281850999707 1106 72-73 0.2704863565857565 1106 74-75 0.1734284924063445 1106 76-77 0.27078018198939446 1106 78-79 0.314033362190294 1106 80-81 0.30020316573438777 1106 82-83 0.39646585739414064 1106 84-85 0.37633784293443995 1106 86-87 0.39117139207002083 1106 88-89 0.3587818331364545 1106 90-91 0.3117304326065451 1106 92-93 0.36527656950202214 1106 94-95 0.32665834897021284 1106 96-97 0.2625566881738095 1106 98-99 0.3555646977819862 1106 100 0.36640283733222745 1107 1 0.46235677985688994 1107 2 3.510759283282553 1107 3 2.110333645044868 1107 4 0.9150809182093482 1107 5 0.33304633512784676 1107 6 0.17889710693042815 1107 7 0.11854284786480918 1107 8 0.1372603792520195 1107 9 0.15943905476974862 1107 10-11 0.13424045443310462 1107 12-13 0.14754157391472944 1107 14-15 0.10638749988934393 1107 16-17 0.0965608485691476 1107 18-19 0.11394924599056822 1107 20-21 0.18382936065744104 1107 22-23 0.08984254833217875 1107 24-25 0.14632763839641427 1107 26-27 0.14064634261956854 1107 28-29 0.092461480159308 1107 30-31 0.2077774440328497 1107 32-33 0.26089318319803567 1107 34-35 0.29714321182672876 1107 36-37 0.13830887802664904 1107 38-39 -0.00470746179847481 1107 40-41 0.14443915582900502 1107 42-43 0.1213021788726465 1107 44-45 0.18825217802817207 1107 46-47 0.15734631376459163 1107 48-49 0.09683497786015494 1107 50-51 0.1007581213866402 1107 52-53 0.04052787252883405 1107 54-55 0.06357139108019538 1107 56-57 0.15497315891960994 1107 58-59 0.16594220085123368 1107 60-61 0.12384408861306184 1107 62-63 0.15345044309668054 1107 64-65 0.11809777876745642 1107 66-67 0.01875628537879237 1107 68-69 -0.014464111464889129 1107 70-71 0.09422349823368137 1107 72-73 0.02646048584139038 1107 74-75 0.1262218005161202 1107 76-77 0.04625367680932868 1107 78-79 0.13335329953044806 1107 80-81 0.1736117875207981 1107 82-83 0.17172855079605398 1107 84-85 0.19863841399869386 1107 86-87 0.1858432861598942 1107 88-89 0.16871890163483272 1107 90-91 0.12792189755919736 1107 92-93 0.20555881285127242 1107 94-95 0.2600960071884799 1107 96-97 0.13023935348650184 1107 98-99 0.19743806691682408 1107 100 0.2808612879243739 1108 1 0.47953049531502856 1108 2 3.689380957967824 1108 3 2.07667999065254 1108 4 0.9546826047324046 1108 5 0.21671463549163406 1108 6 0.08526380385396948 1108 7 -0.0032387666420703454 1108 8 -0.005956602149787216 1108 9 0.0717969446536415 1108 10-11 0.041138179045894674 1108 12-13 0.07930787332679046 1108 14-15 -0.06083028398595047 1108 16-17 -0.06658994656651629 1108 18-19 -0.004030003829598172 1108 20-21 0.06926377891577573 1108 22-23 -0.0583459721921642 1108 24-25 -0.0026319637551708297 1108 26-27 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0.15725658706213608 2210 72-73 0.19822116676126456 2210 74-75 -0.3690343879128619 2210 76-77 0.03835603481322636 2210 78-79 0.28806032084371935 2210 80-81 0.23547668326167326 2210 82-83 0.2947889117301443 2210 84-85 0.2840299481098043 2210 86-87 0.331237415066731 2210 88-89 0.285024785250549 2210 90-91 0.3432472928015997 2210 92-93 0.4223743431049698 2210 94-95 0.3243843117318903 2210 96-97 0.31464946295778873 2210 98-99 0.2387172878360886 2210 100 0.18662810439925082 2211 1 0.5382576418579603 2211 2 3.9801657203936145 2211 3 2.239782976332883 2211 4 1.0431243750234884 2211 5 0.44851308219231356 2211 6 0.39869955937639645 2211 7 0.4091080357112915 2211 8 0.29687955456849835 2211 9 0.3041219260874968 2211 10-11 0.35876383634685993 2211 12-13 0.20959901872944187 2211 14-15 0.27107328387700846 2211 16-17 0.2800975483483157 2211 18-19 0.38824695125435227 2211 20-21 0.38294127267601397 2211 22-23 0.3058054164885533 2211 24-25 0.33221461961853294 2211 26-27 0.36935462877860203 2211 28-29 0.38265752716327484 2211 30-31 0.36584964342748805 2211 32-33 0.3950172115366044 2211 34-35 0.49056966953649805 2211 36-37 0.27410901802272036 2211 38-39 0.27228197604241444 2211 40-41 0.27691465168066287 2211 42-43 0.3054931240063965 2211 44-45 0.3274907607904183 2211 46-47 0.42400442296649743 2211 48-49 0.3814172819257635 2211 50-51 0.19453548120887376 2211 52-53 0.2598164902071929 2211 54-55 0.358662077638499 2211 56-57 0.18043019800548876 2211 58-59 0.29984114668026507 2211 60-61 0.24640006424279903 2211 62-63 0.37324781080577907 2211 64-65 0.29489764435354004 2211 66-67 0.33718345467539024 2211 68-69 0.15006871590027515 2211 70-71 0.1933120867853404 2211 72-73 0.3894287617812182 2211 74-75 0.35575240469063374 2211 76-77 0.1574921335585202 2211 78-79 0.34450973726328726 2211 80-81 0.3737927654649198 2211 82-83 -0.04864546476157727 2211 84-85 0.22595909204835607 2211 86-87 0.33785627545181995 2211 88-89 0.27312709215107844 2211 90-91 0.32661984567443625 2211 92-93 0.2941946746713313 2211 94-95 0.40458637700083955 2211 96-97 0.3376115863464406 2211 98-99 0.3975376338022514 2211 100 0.24564014470419337 2212 1 0.35569638687839245 2212 2 3.9178025339999962 2212 3 2.227838344762329 2212 4 0.9161586904877836 2212 5 0.41224351019260297 2212 6 0.24960837892810162 2212 7 0.119241979388093 2212 8 0.17782332439350057 2212 9 0.07163790109176205 2212 10-11 0.08832549102098852 2212 12-13 0.26261773486146467 2212 14-15 0.134651500830401 2212 16-17 0.1885097387255925 2212 18-19 0.2457661667490072 2212 20-21 0.27250587139187843 2212 22-23 0.23353205403510202 2212 24-25 0.1156268866635699 2212 26-27 0.16489266774014055 2212 28-29 0.0975842276796044 2212 30-31 0.1589054871970106 2212 32-33 0.2860319552567461 2212 34-35 0.25569514398211624 2212 36-37 0.1276070609505311 2212 38-39 -0.07861498556339797 2212 40-41 0.1918020983071287 2212 42-43 0.18288523458787154 2212 44-45 0.2952317300957148 2212 46-47 0.25775336367370727 2212 48-49 0.10035615932584108 2212 50-51 0.17313843117958072 2212 52-53 0.09438741145714147 2212 54-55 0.07410541659072578 2212 56-57 0.07718623201325414 2212 58-59 -0.007455067874950316 2212 60-61 0.08963147716816877 2212 62-63 0.32770287174642476 2212 64-65 0.2748745826932435 2212 66-67 0.06110900721989054 2212 68-69 0.13448952168859307 2212 70-71 0.3766129300284007 2212 72-73 0.19336692872678185 2212 74-75 0.1992426479523033 2212 76-77 0.3499965828438292 2212 78-79 0.13355882200364277 2212 80-81 0.19920710356154103 2212 82-83 0.1670361575689796 2212 84-85 0.2092669887247567 2212 86-87 0.2215620266578071 2212 88-89 0.3581249505651627 2212 90-91 0.11882897077269661 2212 92-93 0.28753142909020113 2212 94-95 0.3492916508222734 2212 96-97 0.16387734540320764 2212 98-99 0.3963088030468285 2212 100 0.27581289248670515 2213 1 -0.5275622519676411 2213 2 -25.972741878607454 2213 3 -14.82950130698993 2213 4 -6.107793749940303 2213 5 -1.9447165466241856 2213 6 -1.1451066799703398 2213 7 -1.1161791260903158 2213 8 -1.0107471945495092 2213 9 -0.9343694948869796 2213 10-11 -1.140733725788941 2213 12-13 -0.9615594012784214 2213 14-15 -1.0938370990765378 2213 16-17 -1.3610910743422622 2213 18-19 -1.2518711609026454 2213 20-21 -1.2417130201909075 2213 22-23 -1.1826711394603535 2213 24-25 -1.1407533695017875 2213 26-27 -1.3626095975844734 2213 28-29 -1.275260652686761 2213 30-31 -1.2115350878280964 2213 32-33 -1.0573839093625566 2213 34-35 -1.083729463565703 2213 36-37 -1.2500243891021299 2213 38-39 -1.3914281513851563 2213 40-41 -1.2832585144325677 2213 42-43 -1.1893137221383014 2213 44-45 -1.0803266418093571 2213 46-47 -1.1824555359907762 2213 48-49 -1.1748177196445937 2213 50-51 -1.274407058987677 2213 52-53 -1.3663881867110064 2213 54-55 -1.1245996748794056 2213 56-57 -1.116957597732295 2213 58-59 -1.1199835374951164 2213 60-61 -1.1232551586411077 2213 62-63 -1.171183445692904 2213 64-65 -1.1783048310162556 2213 66-67 -1.1840027896111067 2213 68-69 -1.3373929018413975 2213 70-71 -1.2883014774361499 2213 72-73 -1.356633683523114 2213 74-75 -1.2781806371357405 2213 76-77 -1.3030738503841945 2213 78-79 -1.157436818777093 2213 80-81 -1.363735859238158 2213 82-83 -1.4558105595470785 2213 84-85 -1.2936724230255479 2213 86-87 -1.228193073383828 2213 88-89 -1.131605169680718 2213 90-91 -1.1612796423588385 2213 92-93 -1.1930878004545242 2213 94-95 -1.285839860455372 2213 96-97 -1.5026393200389592 2213 98-99 -1.3451276576589706 2213 100 -1.349542824702823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 17.0 4 28.0 5 41.0 6 33.0 7 48.0 8 61.0 9 90.0 10 374.0 11 1658.0 12 4788.0 13 8791.0 14 11268.0 15 12949.0 16 13999.0 17 14688.0 18 15512.0 19 16535.0 20 18422.0 21 21002.0 22 23335.0 23 25215.0 24 25845.0 25 27459.0 26 30248.0 27 34797.0 28 40553.0 29 49269.0 30 59918.0 31 73827.0 32 94762.0 33 127626.0 34 178045.0 35 255277.0 36 396354.0 37 682876.0 38 674049.0 39 5194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.237509002312606 20.862420691466646 10.373951699318136 30.526118606902614 2 12.921834356195863 22.89425061908212 36.28052664233039 27.90338838239162 3 15.104780887993194 27.344424617693647 28.83375127973093 28.717043214582226 4 10.605036201112537 18.799313637365405 37.473525357757865 33.122124803764194 5 12.109433723539652 38.44577528932158 34.739846960397315 14.704944026741456 6 29.826381740014963 39.81172499128545 17.601076866730946 12.760816401968638 7 26.005381236597437 33.36648626174576 23.117611711951326 17.51052078970547 8 24.31160560023426 37.013262747245506 20.783875173504125 17.891256479016107 9 24.57953255767107 17.007127165605628 22.134807071251615 36.27853320547169 10-11 23.295232582392533 27.77832129776267 28.82418962868753 20.10225649115727 12-13 23.645101235355494 25.422492665538538 29.44017927796594 21.492226821140033 14-15 21.36646625261275 26.69044368403585 26.56084659679408 25.382243466557323 16-17 20.953244374637396 30.32198161133809 27.12432931112491 21.600444702899605 18-19 20.682094886114008 29.261428721120065 29.304514154422474 20.751962238343456 20-21 22.202945706682605 28.431724510364997 28.40511363539875 20.960216147553652 22-23 21.330489412991408 28.481596172934587 28.891270143846103 21.296644270227908 24-25 21.500499933432778 27.990991009349298 28.811839592010845 21.696669465207076 26-27 21.337724221052692 28.43363559458164 28.63551477407837 21.593125410287296 28-29 20.98166866453753 28.34101660296065 29.05417074434402 21.623143988157796 30-31 21.738925169000563 28.533931386264822 28.346003917504838 21.381139527229784 32-33 20.92990522979796 28.635701631932758 29.02318336738641 21.411209770882873 34-35 21.239984875334418 27.911290134992907 28.990034665502606 21.858690324170073 36-37 21.563104979538725 28.57113022784669 28.421206514058024 21.444558278556563 38-39 21.14348915783592 28.563254291115133 28.81941974204154 21.47383680900741 40-41 21.69723428651377 28.214838209827608 28.4003714400058 21.68755606365282 42-43 21.077646740009857 28.53198779315189 28.778138515091566 21.61222695174668 44-45 21.469902061995707 27.95788733733598 28.70885856719824 21.863352033470075 46-47 21.666860815990933 28.550903405673484 28.24247767594089 21.539758102394693 48-49 21.31616617001653 28.365660171070456 28.48988638947662 21.828287269436395 50-51 21.874649218843796 28.19500180262244 28.068453122391695 21.86189585614207 52-53 21.37381452641175 28.566236734006257 28.32256973244181 21.737379007140184 54-55 21.702293217383804 28.166477287490153 28.198182944869686 21.933046550256353 56-57 21.635030923682642 28.39984758059668 28.08276563784701 21.882355857873662 58-59 21.241731733400893 28.05551017968505 28.615767279178655 22.0869908077354 60-61 21.886803345774144 28.271451907043716 27.896206967985577 21.945537779196567 62-63 21.212700630494155 28.626988559610645 28.344082135919724 21.81622867397548 64-65 21.434645901414413 27.7964553801344 28.42279563348261 22.34610308496858 66-67 21.464164636246245 28.364610977787528 28.112622614864513 22.058601771101713 68-69 21.480637759053103 28.180456792102664 28.32878311953329 22.010122329310942 70-71 21.858189837077017 28.039260473832716 27.885159972250364 22.217389716839904 72-73 21.323747057216224 28.417131735330063 28.35801708296277 21.901104124490942 74-75 21.636656347169836 27.854456035716897 28.158555700658738 22.35033191645453 76-77 21.638147061415655 28.361742560928043 27.9552301433568 22.044880234299498 78-79 21.442073457538736 28.22023269580113 28.280700729589874 22.056993117070256 80-81 21.824362977587455 28.032178459497686 27.919293032941727 22.224165529973135 82-83 21.296457758542672 28.333330220429264 28.372739585935086 21.99747243509298 84-85 21.477521001233253 27.72259575293961 28.204673664785457 22.595209581041676 86-87 21.525513347057455 28.275128077824046 28.020687378722542 22.178671196395957 88-89 21.235977778045676 27.939001859413658 28.351091800632688 22.473928561907982 90-91 21.572344610776664 27.87877038484689 28.034098057809807 22.514786946566634 92-93 21.159425998692967 28.303167255635415 28.28216439180919 22.25524235386243 94-95 21.23460118737055 27.537763881109463 28.426568481453003 22.80106645006699 96-97 21.2714281809262 28.001396979786747 28.18578201240813 22.541392826878923 98-99 21.004937794116923 27.91815516690756 28.36780802043914 22.70909901853638 100 21.481378748648375 27.564945185423888 28.023809693771934 22.929866372155807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 8.0 2 8.0 3 7.0 4 13.0 5 35.5 6 65.5 7 121.5 8 204.5 9 241.0 10 229.5 11 217.0 12 232.0 13 284.5 14 348.0 15 411.0 16 505.0 17 623.0 18 756.0 19 972.5 20 1259.5 21 1761.0 22 2475.0 23 3649.5 24 5419.0 25 7998.5 26 12076.0 27 17985.0 28 26000.5 29 35416.0 30 45526.5 31 56232.0 32 67220.5 33 77854.0 34 87269.5 35 95803.0 36 103981.5 37 111648.0 38 117843.0 39 122063.5 40 124897.0 41 126198.0 42 124706.0 43 120998.0 44 116618.5 45 111204.5 46 104392.5 47 97906.0 48 92922.0 49 88995.5 50 85545.0 51 80756.0 52 74823.0 53 70584.5 54 66856.5 55 62192.5 56 58014.5 57 52775.5 58 47170.0 59 42731.5 60 37904.5 61 32126.5 62 27019.0 63 22115.0 64 17200.5 65 13609.0 66 10424.0 67 7795.5 68 5835.5 69 4391.5 70 3345.5 71 2446.0 72 1864.0 73 1414.0 74 1022.0 75 665.5 76 340.0 77 161.0 78 98.0 79 52.5 80 29.0 81 17.5 82 9.0 83 6.0 84 2.5 85 1.5 86 0.5 87 0.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.844562989926841 2 5.370812117672426 3 0.0 4 0.005297194693976648 5 0.16302456234475568 6 0.05436415836574752 7 0.034703416520795734 8 0.0414607353932403 9 0.03898191992746918 10-11 0.0401024803435027 12-13 0.03893098536310402 14-15 0.0412909535120231 16-17 0.03283581582740653 18-19 0.027317904687847526 20-21 0.022750772083104838 22-23 0.022496099261279035 24-25 0.01911743982505675 26-27 0.012631771962559699 28-29 0.01937211264688255 30-31 0.024923980162684996 32-33 0.0014261678022244823 34-35 0.003276790307491965 36-37 0.004210590654186567 38-39 0.00606121315945405 40-41 0.00677429706056629 42-43 0.007283642704217891 44-45 0.007979748417208412 46-47 0.016502798854311867 48-49 0.024771176469589515 50-51 0.021511364350219272 52-53 0.02624827883617916 54-55 0.023277095914878157 56-57 0.015212456557061143 58-59 0.014057939764784182 60-61 0.011714949803986817 62-63 0.017606381082223666 64-65 0.018642050557648587 66-67 0.01945700358749115 68-69 0.02618036608369228 70-71 0.02623130064805744 72-73 0.022971488528687193 74-75 0.023429899607973634 76-77 0.017606381082223666 78-79 0.014397503527218582 80-81 0.01278457565565518 82-83 0.007589250090408852 84-85 0.010407629318614376 86-87 0.008862614199537855 88-89 0.007113860823000691 90-91 0.007215729951731011 92-93 0.004023830584847647 94-95 0.0015110587428330822 96-97 0.0010356694754249216 98-99 5.60280208016761E-4 100 0.10621554488948047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2944955.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.85758434730583 #Duplication Level Percentage of deduplicated Percentage of total 1 87.91514407371153 71.96521321419385 2 8.809509459530526 14.422503272838172 3 1.809376348834333 4.443335312721801 4 0.5839637336218808 1.9120744232288298 5 0.28743881582205794 1.1764523555421906 6 0.1536292309412789 0.7545430637992478 7 0.0980018499258031 0.5615536287545405 8 0.06314065680969277 0.4134833312434974 9 0.04627205076919878 0.34089464688862564 >10 0.22206792893080127 3.0863565746517594 >50 0.009145243321929088 0.49807946687773563 >100 0.002102512433899726 0.260415413247018 >500 1.248572082263711E-4 0.06731908893387893 >1k 8.323813879308171E-5 0.0977762070788527 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.3956376243440054E-5 0.0 5 0.0 0.0 0.0 3.3956376243440054E-5 0.0 6 0.0 0.0 0.0 3.3956376243440054E-5 0.0 7 0.0 0.0 0.0 3.3956376243440054E-5 0.0 8 0.0 0.0 0.0 3.3956376243440054E-5 0.0 9 0.0 0.0 0.0 6.791275248688011E-5 0.0 10-11 0.0 0.0 0.0 8.489094060860013E-5 0.0 12-13 0.0 0.0 0.0 1.1884731685204018E-4 0.0 14-15 0.0 0.0 0.0 1.5280369309548023E-4 0.0 16-17 0.0 0.0 0.0 1.867600693389203E-4 0.0 18-19 0.0 0.0 3.3956376243440054E-5 2.037382574606403E-4 0.0 20-21 0.0 0.0 3.3956376243440054E-5 2.7165100994752043E-4 0.0 22-23 0.0 0.0 3.3956376243440054E-5 3.3956376243440053E-4 0.0 24-25 0.0 0.0 3.3956376243440054E-5 3.5654195055612056E-4 0.0 26-27 0.0 0.0 3.3956376243440054E-5 5.093456436516008E-4 0.0 28-29 1.6978188121720027E-5 0.0 3.3956376243440054E-5 8.828657823294413E-4 0.0 30-31 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.002631619158866604 0.0 32-33 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.007759031971626052 0.0 34-35 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.016180213279999186 0.0 36-37 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.029185505381236723 0.0 38-39 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.048625530780606156 0.0 40-41 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.07200449582421463 0.0 42-43 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.09679265048192587 0.0 44-45 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.12845697132893372 0.0 46-47 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.16635228721661283 0.0 48-49 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.20686224407503678 0.0 50-51 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.25030942747851836 0.0 52-53 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.2976615941499955 0.0 54-55 3.3956376243440054E-5 0.0 3.3956376243440054E-5 0.351482450495848 0.0 56-57 3.3956376243440054E-5 1.6978188121720027E-5 3.3956376243440054E-5 0.41723897309126967 0.0 58-59 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 0.49029611657903094 0.0 60-61 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 0.5720291141969911 0.0 62-63 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 0.6556976252608274 0.0 64-65 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 0.7492644199996266 0.0 66-67 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 0.8613374397911004 0.0 68-69 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 0.9860761879213774 0.0 70-71 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 1.1241088573509612 0.0 72-73 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 1.2707324899701353 0.0 74-75 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 1.430310480126182 0.0 76-77 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 1.6103471869689012 0.0 78-79 3.3956376243440054E-5 3.3956376243440054E-5 3.3956376243440054E-5 1.8059698705073592 0.0 80-81 5.0934564365160085E-5 3.3956376243440054E-5 3.3956376243440054E-5 2.0248696499606957 0.0 82-83 6.791275248688011E-5 3.3956376243440054E-5 3.3956376243440054E-5 2.255671139287357 0.0 84-85 6.791275248688011E-5 3.3956376243440054E-5 3.3956376243440054E-5 2.506133370458971 0.0 86-87 6.791275248688011E-5 3.3956376243440054E-5 3.3956376243440054E-5 2.7843549392095976 0.0 88 6.791275248688011E-5 3.3956376243440054E-5 3.3956376243440054E-5 3.010096928475987 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5090 0.0 34.584843 1 TCGACGC 75 3.2656698E-4 31.351477 4 ATCAACG 5900 0.0 29.171326 3 GGTATCA 3310 0.0 28.995436 1 TCAACGC 5960 0.0 28.800222 4 CAACGCA 5975 0.0 28.570509 5 TATCAAC 6100 0.0 27.938143 2 AACGCAG 6265 0.0 27.136011 6 ACGCAGA 7100 0.0 23.873243 7 CGCAGAG 7235 0.0 23.297993 8 CCTATAC 750 0.0 20.063921 3 GTACATG 7280 0.0 19.467665 1 GCAGAGT 8655 0.0 19.095165 9 TATACTG 1660 0.0 17.847677 5 AGAGTAC 7850 0.0 17.524508 10-11 TACATGG 7735 0.0 17.343498 2 ACATGGG 7715 0.0 17.12764 3 GACTGCG 365 2.6775524E-9 16.722958 7 GAGTACT 3855 0.0 16.502995 12-13 CGTATAC 175 0.0023215713 16.122795 3 >>END_MODULE