FastQCFastQC Report
Wed 25 May 2016
SRR1295156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561513
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG3351.6370905E-1120.94555
GTCGAGA1906.2897336E-419.5886461
GTATCAA45050.017.7624021
GTACATG69250.017.7358571
TACATGG71000.016.947282
TCAACGC48400.016.6352584
ATCAACG47550.016.4576953
GTATATA16800.016.246141
TATCAAC50050.016.2339362
CAACGCA47100.015.6424185
GTGTACG2804.6901856E-415.5076781
CTATACT10900.015.4964984
GTATAGC5701.4861143E-915.2356131
ACATGGG72600.015.0442263
AACGCAG48100.014.9464276
GTGTATA13850.014.7798811
TATACTG12300.014.66914755
GTATTAG9750.014.6328851
GTATAGG7301.6370905E-1114.4455091
GACTGCG2851.7022154E-414.0503077
Produced by FastQC (version 0.11.5)